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PHM821

Integration plasmid of histone H3 (HHT1) and Venus with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Catalog number RDB18146
Resource name PHM821
Alternative name pTy1-H3-V
Clone info. Expression vector of S. cerevisiae histone H3 (HHT1) with a FLAG-tag and Venus gene with a FLAG-tag at the N-terminus under a synthetic promoter (Psyn) and synthetic terminator (Tguo1). Integration vector harboring S. cerevisiae short Ty1 HR sequence recognized by the gRNA #4, LEU2 marker in pRS405, see Fig.3 and Table S1 of Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019. The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration).
Vector backbone pRS405 (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), LEU2 (S. cerevisiae)
Growth remarks not specify
Gene/insert name S. cerevisiae Ty1 HR genomic DNA S. cerevisiae HHT1 genomic DNA Aequorea victoria GFP cDNA
Depositor|Developer Masumoto, Hiroshi |
Other clones in our bank

External Database
S. cerevisiae Ty1 HR
    S. cerevisiae HHT1 Aequorea victoria GFP

          Reference sequence
            
           
          Sequence (full) RDB18146zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)
          Additional terms and conditions:
          The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)。
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          RDB18146 PHM821 Under QC test. Please contact us. DNA solution

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          How to cite this biological resource

          Materials & Methods section:

          The PHM821 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18146).

          Reference section:

          Hanasaki, M., Masumoto, H., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          Featured content

          Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950. [link to RRC of NBRP]

          2023.11.23

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