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PHM820

Integration plasmid expressing Venus with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Catalog number RDB18144
Resource name PHM820
Alternative name pTy1-V
Clone info. Expression vector of Venus with a FLAG-tag at the N-terminus under a synthetic promoter (Psyn) and synthetic terminator (Tguo1). Integration vector harboring S. cerevisiae short Ty1 HR sequence recognized by the gRNA #4, LEU2 marker in pRS405, see Fig.2 and Table S1 of Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019. The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration).
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pRS405 (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), LEU2 (S. cerevisiae)
Growth remarks not specify
Gene/insert name S. cerevisiae Ty1 HR genomic DNA Aequorea victoria GFP cDNA
Depositor|Developer Masumoto, Hiroshi |
Other clones in our bank
External Database
S. cerevisiae Ty1 HR
    Aequorea victoria GFP
            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)
            Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form             [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)。
            付加的提供条件 利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB18144 PHM820 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Sequence (full) RDB18144zzk01.seq provided by the depositor

            Original reference

            original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

            2022.05.16

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