Resource data sheet
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PHM820 (#RDB18144)

Integration plasmid expressing Venus with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Alternative name pTy1-V
Clone info. Expression vector of Venus with a FLAG-tag at the N-terminus under a synthetic promoter (Psyn) and synthetic terminator (Tguo1). Integration vector harboring S. cerevisiae short Ty1 HR sequence recognized by the gRNA #4, LEU2 marker in pRS405, see Fig.2 and Table S1 of Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019. The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration).
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pRS405 (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), LEU2 (S. cerevisiae)
Growth conditions LB+Ampicillin, 37oC
Growth remarks not specify
Gene/insert name S. cerevisiae Ty1 HR genomic DNA Aequorea victoria GFP cDNA
Depositor|Developer Masumoto, Hiroshi |
 
Sequence (full) RDB18144zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)
Additional terms and conditions:
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)。
付加的使用条件：
本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18144 PHM820 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The PHM820 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18144).

Reference section:

Hanasaki, M., Masumoto, H., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950. [link to RRC of NBRP]