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PHM829

Integration plasmid of histone H3 (HHT1) with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Catalog number RDB18143
Resource name PHM829
Alternative name pTy1-H3 ∆p
Clone info. Integration vector of S. cerevisiae histone H3 (HHT1) with a FLAG-tag at the N-terminus without promoter harboring S. cerevisiae short Ty1 HR sequence recognized by the gRNA #4, HIS3 marker in pRS403, see Fig.2 and Table S1 of Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019. The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration).
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pRS403 (plasmid)
Size of vector backbone 4.5 kb
Selectable markers Ampicillin (E. coli), HIS3 (S. cerevisiae)
Growth remarks not specify
Gene/insert name S. cerevisiae Ty1 HR genomic DNA S. cerevisiae HHT1 genomic DNA
Depositor|Developer Masumoto, Hiroshi |
Other clones in our bank

External Database
S. cerevisiae Ty1 HR
    S. cerevisiae HHT1

          Reference sequence
            
           
          Sequence (full) RDB18143zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)。
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          RDB18143 PHM829 Under QC test. Please contact us. DNA solution

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          References and tips

          Original reference

          original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

          2022.07.27

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