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PHM829

Integration plasmid of histone H3 (HHT1) with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Catalog number RDB18143
Resource name PHM829
Alternative name pTy1-H3 ∆p
Clone info. Integration vector of S. cerevisiae histone H3 (HHT1) with a FLAG-tag at the N-terminus without promoter harboring S. cerevisiae short Ty1 HR sequence recognized by the gRNA #4, HIS3 marker in pRS403, see Fig.2 and Table S1 of Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019. The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration).
Expression was confirmed by the depositor with western blotting.
Vector backbone pRS403 (plasmid)
Size of vector backbone 4.5 kb
Selectable markers Ampicillin (E. coli), HIS3 (S. cerevisiae)
Growth remarks not specify
Gene/insert name S. cerevisiae Ty1 HR genomic DNA S. cerevisiae HHT1 genomic DNA
Depositor|Developer Masumoto, Hiroshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)
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RDB18143 PHM829 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB18143zzk01.seq

Original reference

original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

2020.10.26

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