Resource data sheet
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PHM757 (#RDB18142)

Integration plasmid expressing histone H3 (HHT1) with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Alternative name pTy1-H3
Clone info. Expression vector of S. cerevisiae histone H3 (HHT1) with a FLAG-tag at the N-terminus driven by the TDH3 promoter. Integration vector harboring S. cerevisiae short Ty1 HR sequence recognized by the gRNA #4, HIS3 marker in pRS403, see Table S1 of Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019. The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration).
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pRS403 (plasmid)
Size of vector backbone 4.5 kb
Selectable markers Ampicillin (E. coli), HIS3 (S. cerevisiae)
Growth remarks not specify
Gene/insert name S. cerevisiae Ty1 HR genomic DNA S. cerevisiae HHT1 genomic DNA
Depositor|Developer Masumoto, Hiroshi |
 
Sequence (full) RDB18142zzk01.seq provided by the depositor

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18142 PHM757 Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The PHM757 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18142).

Reference section:

Hanasaki, M., Masumoto, H., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950. [link to RRC of NBRP]