Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

PHM757

Integration plasmid expressing histone H3 (HHT1) with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Catalog number RDB18142
Resource name PHM757
Alternative name pTy1-H3
Clone info. Expression vector of S. cerevisiae histone H3 (HHT1) with a FLAG-tag at the N-terminus driven by the TDH3 promoter. Integration vector harboring S. cerevisiae short Ty1 HR sequence recognized by the gRNA #4, HIS3 marker in pRS403, see Table S1 of Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019. The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration).
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pRS403 (plasmid)
Size of vector backbone 4.5 kb
Selectable markers Ampicillin (E. coli), HIS3 (S. cerevisiae)
Growth remarks not specify
Gene/insert name S. cerevisiae Ty1 HR genomic DNA S. cerevisiae HHT1 genomic DNA
Depositor|Developer Masumoto, Hiroshi |
Other clones in our bank

External Database
S. cerevisiae Ty1 HR
    S. cerevisiae HHT1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB18142 PHM757 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Sequence (full) RDB18142zzk01.seq provided by the depositor

          Original reference

          original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

          2022.05.16

          GNP_filter3_RDBDEP_html_220516.pl