RDB18138 - PHM664

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PHM664

Cas9 and sgRNA #4 plasmid helping pTy1 integration into Ty1 loci. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Catalog number	RDB18138
Resource name	PHM664
Clone info.	The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration). Expression vector of sgRNA (gTy1 #4) recognizing short Ty1 HR sequence and Cas9 in pML104, see Table S1 of Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019.
Vector backbone	pML104 (plasmid)
Size of vector backbone	11.2 kb
Selectable markers	Ampicillin (E. coli), LEU2 (S. cerevisiae)
Growth remarks	not specify
Depositor\|Developer	Masumoto, Hiroshi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)

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Exclusive MTA (genome editing, for use for not-for-profit academic purpose) [Word]

Remarks
The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (genome editing).

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA (genome editing, for use for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (genome editing).

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 専用MTA（ゲノム編集、非営利機関による非営利学術研究用）をお使いください [Word]
備考	本件リソースは商業目的には利用できません。専用MTA（ゲノム編集）をご参照ください。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB18138	PHM664	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB18138zzk01.seq

Original reference

original	Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

Further references such as user reports and related articles (go to bottom)

Featured content	Expression Vector Backbone of Saccharomyces cerevisiae (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

2020.10.26

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