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PHM664

Cas9 and sgRNA #4 plasmid helping pTy1 integration into Ty1 loci. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Catalog number RDB18138
Resource name PHM664
Clone info. The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration). Expression vector of sgRNA (gTy1 #4) recognizing short Ty1 HR sequence and Cas9 in pML104, see Table S1 of Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019.
Vector backbone pML104 (plasmid)
Size of vector backbone 11.2 kb
Selectable markers Ampicillin (E. coli), LEU2 (S. cerevisiae)
Growth remarks not specify
Depositor|Developer Masumoto, Hiroshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)
Additional terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18138 PHM664 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB18138zzk01.seq

Original reference

original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

2021.03.11

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