Cas9 and sgRNA #4 plasmid helping pTy1 integration into Ty1 loci. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.
Catalog number | RDB18138 |
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Resource name | PHM664 |
Clone info. | The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration). Expression vector of sgRNA (gTy1 #4) recognizing short Ty1 HR sequence and Cas9 in pML104, see Table S1 of Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019. |
Vector backbone | pML104 (plasmid) |
Size of vector backbone | 11.2 kb |
Selectable markers | Ampicillin (E. coli), LEU2 (S. cerevisiae) |
Growth remarks | not specify |
Depositor|Developer | Masumoto, Hiroshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019) |
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Additional terms and conditions for distribution | The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. |
Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word ![]() |
Remarks | The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose). |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB18138 | PHM664 | Under QC test. Please contact us. | DNA solution |
Electronic file
Electronic file | Nucleotide sequence provided by the depositor RDB18138zzk01.seq |
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Original reference
original | Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950. |
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Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Expression Vector Backbone of Saccharomyces cerevisiae (English text) |
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Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950. |
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2021.03.11
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