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PHM663

Cas9 and sgRNA plasmid helping pTy1 integration into Ty1 loci. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Catalog number RDB18137
Resource name PHM663
Clone info. The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration). Expression vector of sgRNA (gTy1 #3) recognizing Ty1 HR sequence and Cas9 in pML104, see Fig. 1 and Table S1 of Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019.
Vector backbone pML104 (plasmid)
Size of vector backbone 11.2 kb
Selectable markers Ampicillin (E. coli), LEU2 (S. cerevisiae)
Growth remarks not specify
Depositor|Developer Masumoto, Hiroshi |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB18137zzk01.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)
            Additional terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
            Remarks
            The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)。
            付加的提供条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
            備考
            本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB18137 PHM663 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Original reference

            original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

            2022.07.27

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