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PHM688

Integration plasmid to Ty1 loci, pRS403 back bone. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Catalog number RDB18136
Resource name PHM688
Clone info. The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration). Integration vector harboring S. cerevisiae Ty1 HR sequence (YPRWRTy1-3, the region from 1681 bp to 2541 bp), HIS3 marker in pRS403, see Table S1 of Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019.
Comment Genomic DNA was obtained from BY4742 strain by a PCR.
Vector backbone pRS403 (plasmid)
Size of vector backbone 4.5 kb
Selectable markers Ampicillin (E. coli), HIS3 (S. cerevisiae)
Growth remarks not specify
Gene/insert name S. cerevisiae Ty1 HR genomic DNA
Depositor|Developer Masumoto, Hiroshi |
Other clones in our bank

External Database
S. cerevisiae Ty1 HR

            Reference sequence
              
             
            Sequence (full) RDB18136zzk01.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)。
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            RDB18136 PHM688 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Original reference

            original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

            2022.07.27

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