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PHM661

Integration plasmid to Ty1 loci, YIplac128 back bone (pTy1). CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Catalog number RDB18135
Resource name PHM661
Alternative name pTy1
Clone info. The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration). Integration vector harboring S. cerevisiae Ty1 HR sequence (YPRWRTy1-3, the region from 1681 bp to 2541 bp), LEU2 marker in YIplac128. See Fig. 1 and Table S1 of Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019.
Genomic DNA was obtained from BY4742 strain by a PCR.
Vector backbone YIplac128 (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Ampicillin (E. coli), LEU2 (S. cerevisiae)
Growth remarks not specify
Gene/insert name S. cerevisiae Ty1 HR genomic DNA
Depositor|Developer Masumoto, Hiroshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)
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RDB18135 PHM661 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB18135zzk01.seq

Original reference

original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

2020.10.26

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