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pUC18Z_ePAG2 (#RDB17948)

Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS).

Clone info. Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS) when 4-chloro-phenylalanine (4CP) is present. Zeocin resistance. See Fig. S2 of Miyazaki, K. Biotechniques, 58 (2): 86-88, 2015 for constructs.
Comment Causing host cell death in the presence of 1 to 10% (w/v) of 4-chlorophenylalanine.
Vector backbone pUC18 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Zeocin
Gene/insert name Escherichia coli pheS genomic DNA Streptoalloteichus hindustanus zeocin resistance gene genomic DNA
Depositor|Developer Miyazaki, Kentaro |
 
Sequence (full) RDB17948hts01.seq checkAssembled from experimentally sequenced data.
Publication Miyazaki, K., Biotechniques 58 (2): 86-88 (2015). [link to RRC of NBRP]
Test sheet RDB20283_B4D8p1-1.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Miyazaki K. et al. Biotechniques, 58 (2): 86-88, 2015)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyazaki K. et al. Biotechniques, 58 (2): 86-88, 2015)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17948 pUC18Z_ePAG2 DNA solution

How to cite this biological resource

Materials & Methods section:

The pUC18Z_ePAG2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17948).

Reference section:

Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032. [link to RRC of NBRP]