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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pUC18Z_ePAG2

Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS).

Catalog number RDB17948
Resource name pUC18Z_ePAG2
Clone info. Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS) when 4-chloro-phenylalanine (4CP) is present. Zeocin resistance. See Fig. S2 of Miyazaki, K. Biotechniques, 58 (2): 86-88, 2015 for constructs.
Comment Causing host cell death in the presence of 1 to 10% (w/v) of 4-chlorophenylalanine.
Vector backbone pUC18 (plasmid)
Selectable markers Ampicillin
Gene/insert name Escherichia coli pheS genomic DNA Streptoalloteichus hindustanus zeocin resistance gene genomic DNA
Depositor|Developer Miyazaki, Kentaro |
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External Database
Escherichia coli pheS Streptoalloteichus hindustanus zeocin resistance gene

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Miyazaki K. et al. Biotechniques, 58 (2): 86-88, 2015)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyazaki K. et al. Biotechniques, 58 (2): 86-88, 2015)。
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          RDB17948 pUC18Z_ePAG2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB17948zzk01.seq provided by the depositor

          Original reference

          original Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17948_B0Bcp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: ZeoR-C_F
          Region: insert mid (ZeoR 3', pheS 5')
          Sequence file: RDB17948_B0Bca.seq check
          >D06895A6_B0Bc_2_ZeoR-C_F_D03_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGNNNNNNGC CGNNNNNNGC GTGCNCTTCG TGGCCGANGA
             61 NCAGGACTAA AGGAGGTAAA ACACATGTCA CATCTCGCAG AACTGGTTGC CAGTGCGAAG
            121 GCGGCCATTA GCCAGGCGTC AGATGTTGCC GCGTTAGATA ATGTGCGCGT CGAATATTTG
            181 GGTAAAAAAG GGCACTTAAC CCTTCAGATG ACGACCCTGC GTGAGCTGCC GCCAGAAGAG
            241 CGTCCGGCAG CTGGTGCGGT TATCAACGAA GCGAAAGAGC AGGTTCAGCA GGCGCTGAAT
            301 GCGCGTAAAG CGGAACTGGA AAGCGCTGCA CTGAATGCGC GTCTGGCGGC GGAAACGATT
            361 GATGTCTCTC TGCCAGGTCG TCGCATTGAA AACGGCGGTC TGCATCCGGT TACCCGTACC
            421 ATCGACCGTA TCGAAAGTTT CTTCGGTGAG CTTGGCTTTA CCGTGGCAAC CGGGCCGGAA
            481 ATCGAAGACG ATTATCATAA CTTCGATGCT CTGAACATTC CTGGTCACCA CCCGGCGCGC
            541 GCTGACCACG ACACTTTCTG GTTTGACACT ACCCGCCTGC TGCGTACCCA GACCTCTGGC
            601 GTACAGATCC GCACCATGAA AGCCCAGCAG CCACCGATTC GTATCATCGC GCCTGGCCGT
            661 GTTTATCGTA ACGACTACGA CCAGACTCAC ACGCCGATGT TCCATCAGAT GGAAGGTCTG
            721 ATTGTTGATA CCNACATCAG CTTTACCAAC CTGAAAGGNA CGCTGCACGA CTTCCTGCGT
            781 AACTTCTTTG ANGAAGANTT GCAGANTCGC TTCCGTCCTT CCTACTTCNC GTTTGCTGAN
            841 CCTTCTGCAG AAGTGGANGT CNTGGGGTAA AAACGGTAAA TGGCTGGAAN TGCTGGGCTG
            901 CGGGNNGGNG NATNCNNAAC GNGTTGCGNN NCGTNNNCNT CNANCCNGNA NNTTTACTCT
            961 GGNTTCGGNT NCNGGANNGG GANGGANCGN CNGANTATGN NGCGNNNNNG CGNNNNNNAN
           1021 NTGNGNNCNT TCNNNNNAAA CNNNNNNNNN TNNNNNNNNA GNTNNANNNN NNNNCNNGNN
          1081 NNNNNNNN
          //
          Primer: Zeo-M_R
          Region: insert 5' (ZeoR 5')
          Sequence file: RDB17948_B0Bcb.seq check
          >D06895A6_B0Bc_2_Zeo-M_R_F03_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGCGNNNCC NCNCCCNGGC NNGGGTGTTG TCCGGCACCA
             61 CCTGGTCCTG GACCGCGCTG ATGAACAGGG TCACGTCGTC CCGGACCACA CCGGCGAAGT
            121 CGTCCTCCAC GAAGTCCCGA GAGAACCCGA GCCGGTCGGT CCAGAACTCG ACCGCTCCGG
            181 CGACGTCGCG CGCGGTGAGC ACCGGAACGG CACTGGTCAA CTTGGCCATA CTCTTCCTTT
            241 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT
            301 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG
            361 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
            421 CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG
            481 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT
            541 CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC
            601 TGAGAGTGCA CCATGTGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
            661 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
            721 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GANGTGCTGC AAGGCGATTA AGTTGGGTAA
            781 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCNAG CTTGCNTGNC
            841 TGCANGNCGA NTCTAGAGGN NCCCCNGNTA NCGANCTCGA ATTCNTAATC NTGGNCNTAT
            901 GTGTTTCCTG NGTGAAATTG NNATCCGCTC NNNATTCCNC NCNNNNTACN ANCCNNNNNN
            961 NTAAAGNGNA AANNNTGGGN NGNNTNNNGA NNGANCTNNC TCNNNNTNNN NNGNNNNNNN
          1021 NNN
          //
          Primer: ePAG_R1
          Region: insert 3' (pheS 3')
          Sequence file: RDB17948_B0Bcc.seq check
          >D06895A6_B0Bc_2_ePAG_R1_E03_15_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTNNCCTAGN TCCTTTTAAA TTAAAAATGA AGTTTTAAAT
             61 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGGAA TTATTATTTA AACTGTTTGA
            121 GGAAACGCAG ATCGTTTTCG AAGAATGAAC GCAGGTCGGT GACGCCGTAA CGCAACATAG
            181 TCAGACGCTC CATCCCCATC CCGAACCCGA AACCAGAGTA AACTTCCGGG TCGATGCCAA
            241 CGTTACGCAA CACGTTCGGA TGCACCATCC CGCAGCCCAG CACTTCCAGC CATTTACCGT
            301 TTTTACCCAT GACGTCCACT TCTGCAGAAG GTTCAGCAAA CGGGAAGTAG GAAGGACGGA
            361 AGCGAATCTG CAAATCTTCC TCAAAGAAGT TACGCAGGAA GTCGTGCAGC GTGCCTTTCA
            421 GGTTGGTAAA GCTGATGTTG GTATCAACAA TCAGACCTTC CATCTGATGG AACATCGGCG
            481 TGTGAGTCTG GTCGTAGTCG TTACGATAAA CACGGCCAGG CGCGATGATA CGAATCGGTG
            541 GCTGCTGGGC TTTCATGGTG CGGATCTGTA CGCCAGAGGT CTGGGTACGC AGCAGGCGGG
            601 TAGTGTCAAA CCAGAAAGTG TCGTGGTCAG CGCGCGCCGG GTGGTGACCA GGAATGTTCA
            661 GAGCATCGAA GTTATGATAA TCGTCTTCGA TTTCCGGCCC GGTTGCCACG GTAAAGCCNA
            721 GCTCACCGAA GAAACTTTCG ATACGGTCGA TGGTACGGGT AACCGGANGC AGACCGCCGT
            781 TTTCAATGCG ACGACCTGGC AGANAGACAT CNATCGTTTC CGCCGCCAGA NGCGCATTCA
            841 GTGCAGCGCT TTCCAGTTCC GCTTTACGCG CATTCNGCGN CNGNTGAACC NGCTCTTTNG
            901 CTNCNTTGAN AANCGCACCA GCTGCNNGAN GCTCNNCTGG NGGNNGCTCA CGNNNNNNGN
          961 CATNNGNNNN NNNNAGNGNC NNTTTTTANC NNNNANTNNA NNNNCANANN NNNNNNNN
          //
          Primer: SV40pA3'Element Reverse
          Region: ColE1 ori, MCS
          Sequence file: RDB17948_B0Bcd.seq check
          >17948_17948_B0Bc_1_SV40pA3'elementReverse_H01_08_ABI08.ab1
              1 NNNNNNNNNN GNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NNNCGCGGCC TTTNNNNNNT
             61 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG
            121 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG
            181 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC
            241 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG
            301 GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC
            361 ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG
            421 GAAACACATA TGACCATGAT TACGAATTCG AGCTCGGTAC CCGGGGATCC TCTAGAGTCG
            481 ACCTGCAGGC ATGCAAGCTT GGCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC
            541 NNNNNNNNNA CCCAACTTAA TCGCCTTGCA NCACATCCCC CTTTCGCCAG CTGGCGNNAA
            601 TANCGAAGAN GGNNNNNNCC NATCGCCCNT TCCNNNANAG NTNGCNCNNC CNGAATGGGN
            661 NANNGGGCGC CTGATGCNNN NNTTTTTCNC CTTTNNNCNN CTGNGNNGNA TTNNANACNN
          721 CNNNNNGNNG NNCNNNNNNN ANNNNCTGCN NNNNNANGNC NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl