Please review the QC test results indicated by icon below as well as clone information before placing your order.

pUC18K_ePAG2

Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Miyazaki K. et al. Biotechniques, 58 (2): 86-88, 2015)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyazaki K. et al. Biotechniques, 58 (2): 86-88, 2015)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17947_B0Bcp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: KanR-C_F Region: insert mid (KmR 3', pheS 5') Sequence file: RDB17947_B0Bca.seq
>D06895A5_B0Bc_1_kanR-C_F_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGTA NTGNNAATCC TGATATGAAT AAATTGCAGT    61 TTCATTTGAT GCTCGATGAG TTTTTCTAAA GGAGGTAAAA CACATGTCAC ATCTCGCAGA   121 ACTGGTTGCC AGTGCGAAGG CGGCCATTAG CCAGGCGTCA GATGTTGCCG CGTTAGATAA   181 TGTGCGCGTC GAATATTTGG GTAAAAAAGG GCACTTAACC CTTCAGATGA CGACCCTGCG   241 TGAGCTGCCG CCAGAAGAGC GTCCGGCAGC TGGTGCGGTT ATCAACGAAG CGAAAGAGCA   301 GGTTCAGCAG GCGCTGAATG CGCGTAAAGC GGAACTGGAA AGCGCTGCAC TGAATGCGCG   361 TCTGGCGGCG GAAACGATTG ATGTCTCTCT GCCAGGTCGT CGCATTGAAA ACGGCGGTCT   421 GCATCCGGTT ACCCGTACCA TCGACCGTAT CGAAAGTTTC TTCGGTGAGC TTGGCTTTAC   481 CGTGGCAACC GGGCCGGAAA TCGAAGACGA TTATCATAAC TTCGATGCTC TGAACATTCC   541 TGGTCACCAC CCGGCGCGCG CTGACCACGA CACTTTCTGG TTTGACACTA CCCGCCTGCT   601 GCGTACCCAG ACCTCTGGCG TACAGATCCG CACCATGAAA GCCCAGCAGC CACCGATTCG   661 TATCATCGCG CCTGGCCGTG TTTATCGTAA CGACTACGAC CAGACTCACA CGCCGATGTT   721 CCATCAGATG GAAGGTCTGA TTGTTGATAC CAACATCAGC TTTACCAACC TGAAAGGCAC   781 GCTGCACGAC TTCCTGCGTA ACTTCTTTGA GGAAGATTTG CAGANTCGCT TCCGTCCTTC   841 CTACTTCCCG TTTGCTGANN CTTCTGCANA ANNGGACGTC NTGGGTAAAA ACNGTAAATG   901 GCTGGAAGTG CTGGGCTGCG GGATNNNGNA TNCNANNGTG NTGCGTANCG TTGNNATCNA   961 NCNNNAAGTT TACTCNGGNN TTNNGGNTCG GGATNGGGAT GGNNCGTCNN NNTATGNNGC 1021 GNNNNNNNNN N //
Primer: KanR-N_R Region: insert 5' (KmR 5', AmpR pro, MCS) Sequence file: RDB17947_B0Bcb.seq
>D06895A5_B0Bc_1_kanR-N_R_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCATCN NNGTTGGAAT TTAATCGCGG    61 CCTCGAGCAA GACGTTTCCC GTTGAATATG GCTCATACTC TTCCTTTTTC AATATTATTG   121 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA   181 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC   241 CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC   301 GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC   361 TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG   421 CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA   481 TGTGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC   541 GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG   601 CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC   661 CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGCCAAGCTT GCATGCCTGC AGGTCGACTC   721 TAGAGGATCC CCGGGTACCG AGCTCGANTT CGTAATCATG GTCATATGTG TTTCCTGTGT   781 GAAATTGTTA TCCGCTCNCN ATTCCACACN ACATACNAGC CGGAANCATA AAGTGTAAAG   841 CCTGGGGGNG CCTAATGAGT GAGCTNNCTC ACATTNNTTG CGTNGCGCNC NCTGCCNGCT   901 TTCCANNNNG GNAANCNGTC NNGNCNNCTG CNTTAATGNA NCGGNNNACG CNNGGGGNNA   961 NGNNGNTTGN GNANNNNGCG CTCTTCCGNT NNNNCGCNCN NNNANNNNNT NCNNNNNNNC 1021 NTNNNNNNNC NNCNNNNNGN NANNNNNNCN NNNNANNNNN NNNNN //
Primer: ePAG_R1 Region: insert 3' (pheS 3') Sequence file: RDB17947_B0Bcc.seq
>D06895A5_B0Bc_1_ePAG_R1_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTNNCCTNN NNNNTTTTNN TTANNNNNGA AGTTTTAAAT    61 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGGAA TTATTATTTA AACTGTTTGA   121 GGAAACGCAG ATCGTTTTCG AAGAATGAAC GCAGGTCGGT GACGCCGTAA CGCAACATAG   181 TCAGACGCTC CATCCCCATC CCGAACCCGA AACCAGAGTA AACTTCCGGG TCGATGCCAA   241 CGTTACGCAA CACGTTCGGA TGCACCATCC CGCAGCCCAG CACTTCCAGC CATTTACCGT   301 TTTTACCCAT GACGTCCACT TCTGCAGAAG GTTCAGCAAA CGGGAAGTAG GAAGGACGGA   361 AGCGAATCTG CAAATCTTCC TCAAAGAAGT TACGCAGGAA GTCGTGCAGC GTGCCTTTCA   421 GGTTGGTAAA GCTGATGTTG GTATCAACAA TCAGACCTTC CATCTGATGG AACATCGGCG   481 TGTGAGTCTG GTCGTAGTCG TTACGATAAA CACGGCCAGG CGCGATGATA CGAATCGGTG   541 GCTGCTGGGC TTTCATGGTG CGGATCTGTA CGCCAGAGGT CTGGGTACGC AGCAGGCGGG   601 TAGTGTCAAA CCAGAAAGTG TCGTGGTCAG CGCGCGCCGG GTGGTGACCA GGAATGTTCA   661 GAGCATCGAA GTTATGATAA TCGTCTTCGA TTTCCGGCCC GGTTGCCACG GTAAAGCCAA   721 GCTCACCGAA GAAACTTTCG ATACGGTCGA TGGTACGGGT AACCGGATGC AGACCGCCGT   781 TTTCNATGCG ACGANCTGGC AGAGAGACAT CNANNCGTTT CCNCCGCCAG ANGCGCATTC   841 AGTGCAGCGC TTTCCAGTTC CGCTTTACNC GCATTCAGCG NCNGCTGANN CTGCTCTTTC   901 NCTNCNTTGA TAACCGCACN NGCTGCNNNA NGCTCNNCTN NNNNNAGCTC ACGCANGNNN   961 GTCNTCTGNN NGGNNNANTG NCCTTTTTTA NNNNNNNNTN NNNNNGNNNA TNNTCTNNNG 1021 NNGCANCNTC NNNNNNNNNN //
Primer: SV40pA3'Element Reverse Region: ColE1 ori, MCS Sequence file: RDB17947_B0Bcd.seq
>17947_17947_B0Bc_1_SV40pA3'elementReverse_G01_07_ABI08.ab1     1 NNNNNNNNNN NGNNNNNNNN NNNNNNNNNA NGCNNNNNNN NCGGCCTTTT TACGGTTCCT    61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA   121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG   181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC   241 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG   301 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT   361 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA   421 CACATATGAC CATGATTACG AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCGACCT   481 GCAGGCATGC AAGCTTGGCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG   541 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG   601 AAGANGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAN CCTGNATGGC NANNGGNGCC   661 TGATGCGGNA TTTTCTCNTT ANNCATCTGN GCNNNATTTC ACANCGCANN TGGNGCNNTC 721 TCNNNNCAAT CTGCTCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.01.06

GNP_filter3_RDBDEP_html_201222.pl