Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS).
Drawn by SnapGene® software |
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Clone info. | Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS) when 4-chloro-phenylalanine (4CP) is present. Kanamycin resistance. See Fig. S2 of Miyazaki, K. Biotechniques, 58 (2): 86-88, 2015 for constructs. |
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Comment | Causing host cell death in the presence of 1 to 10% (w/v) of 4-chlorophenylalanine. |
Vector backbone | pUC18 (plasmid) |
Size of vector backbone | 2.7 kb |
Selectable markers | Kanamycin |
Gene/insert name | Escherichia coli pheS genomic DNA E. coli kanamycin resistance gene genomic DNA |
Depositor|Developer | Miyazaki, Kentaro | |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Miyazaki K. et al. Biotechniques, 58 (2): 86-88, 2015) |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyazaki K. et al. Biotechniques, 58 (2): 86-88, 2015)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB17947 | pUC18K_ePAG2 | DNA solution |
Materials & Methods section:
The pUC18K_ePAG2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17947). |
Reference section:
Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032. [link to RRC of NBRP] |
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