Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS).
Catalog number | RDB17947 |
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Resource name | pUC18K_ePAG2 |
Clone info. | Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS) when 4-chloro-phenylalanine (4CP) is present. Kanamycin resistance. See Fig. S2 of Miyazaki, K. Biotechniques, 58 (2): 86-88, 2015 for constructs. Causing host cell death in the presence of 1 to 10% (w/v) of 4-chlorophenylalanine. |
Vector backbone | pUC18 (plasmid) |
Selectable markers | Ampicillin |
Gene/insert name | Escherichia coli pheS genomic DNA E. coli kanamycin resistance gene genomic DNA |
Depositor|Developer | Miyazaki, Kentaro | |
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Other clones in our bank | Escherichia coli (KEGG orthology K01889) | E. coli (KEGG orthology K19272) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Miyazaki K. et al. Biotechniques, 58 (2): 86-88, 2015) |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB17947 | pUC18K_ePAG2 | DNA solution |
Electronic file
Electronic file | Nucleotide sequence provided by the depositor RDB17947zzk01.seq |
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Original reference
original | Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032. |
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Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Empty Backbone (English text) |
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Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB17947_B0Bcp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: KanR-C_F Region: insert mid (KmR 3', pheS 5') Sequence file: RDB17947_B0Bca.seq ![]() |
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>D06895A5_B0Bc_1_kanR-C_F_B03_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGTA NTGNNAATCC TGATATGAAT AAATTGCAGT 61 TTCATTTGAT GCTCGATGAG TTTTTCTAAA GGAGGTAAAA CACATGTCAC ATCTCGCAGA 121 ACTGGTTGCC AGTGCGAAGG CGGCCATTAG CCAGGCGTCA GATGTTGCCG CGTTAGATAA 181 TGTGCGCGTC GAATATTTGG GTAAAAAAGG GCACTTAACC CTTCAGATGA CGACCCTGCG 241 TGAGCTGCCG CCAGAAGAGC GTCCGGCAGC TGGTGCGGTT ATCAACGAAG CGAAAGAGCA 301 GGTTCAGCAG GCGCTGAATG CGCGTAAAGC GGAACTGGAA AGCGCTGCAC TGAATGCGCG 361 TCTGGCGGCG GAAACGATTG ATGTCTCTCT GCCAGGTCGT CGCATTGAAA ACGGCGGTCT 421 GCATCCGGTT ACCCGTACCA TCGACCGTAT CGAAAGTTTC TTCGGTGAGC TTGGCTTTAC 481 CGTGGCAACC GGGCCGGAAA TCGAAGACGA TTATCATAAC TTCGATGCTC TGAACATTCC 541 TGGTCACCAC CCGGCGCGCG CTGACCACGA CACTTTCTGG TTTGACACTA CCCGCCTGCT 601 GCGTACCCAG ACCTCTGGCG TACAGATCCG CACCATGAAA GCCCAGCAGC CACCGATTCG 661 TATCATCGCG CCTGGCCGTG TTTATCGTAA CGACTACGAC CAGACTCACA CGCCGATGTT 721 CCATCAGATG GAAGGTCTGA TTGTTGATAC CAACATCAGC TTTACCAACC TGAAAGGCAC 781 GCTGCACGAC TTCCTGCGTA ACTTCTTTGA GGAAGATTTG CAGANTCGCT TCCGTCCTTC 841 CTACTTCCCG TTTGCTGANN CTTCTGCANA ANNGGACGTC NTGGGTAAAA ACNGTAAATG 901 GCTGGAAGTG CTGGGCTGCG GGATNNNGNA TNCNANNGTG NTGCGTANCG TTGNNATCNA 961 NCNNNAAGTT TACTCNGGNN TTNNGGNTCG GGATNGGGAT GGNNCGTCNN NNTATGNNGC 1021 GNNNNNNNNN N // |
Primer: KanR-N_R Region: insert 5' (KmR 5', AmpR pro, MCS) Sequence file: RDB17947_B0Bcb.seq ![]() |
>D06895A5_B0Bc_1_kanR-N_R_A03_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCATCN NNGTTGGAAT TTAATCGCGG 61 CCTCGAGCAA GACGTTTCCC GTTGAATATG GCTCATACTC TTCCTTTTTC AATATTATTG 121 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 181 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC 241 CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC 301 GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC 361 TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG 421 CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA 481 TGTGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC 541 GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG 601 CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC 661 CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGCCAAGCTT GCATGCCTGC AGGTCGACTC 721 TAGAGGATCC CCGGGTACCG AGCTCGANTT CGTAATCATG GTCATATGTG TTTCCTGTGT 781 GAAATTGTTA TCCGCTCNCN ATTCCACACN ACATACNAGC CGGAANCATA AAGTGTAAAG 841 CCTGGGGGNG CCTAATGAGT GAGCTNNCTC ACATTNNTTG CGTNGCGCNC NCTGCCNGCT 901 TTCCANNNNG GNAANCNGTC NNGNCNNCTG CNTTAATGNA NCGGNNNACG CNNGGGGNNA 961 NGNNGNTTGN GNANNNNGCG CTCTTCCGNT NNNNCGCNCN NNNANNNNNT NCNNNNNNNC 1021 NTNNNNNNNC NNCNNNNNGN NANNNNNNCN NNNNANNNNN NNNNN // |
Primer: ePAG_R1 Region: insert 3' (pheS 3') Sequence file: RDB17947_B0Bcc.seq ![]() |
>D06895A5_B0Bc_1_ePAG_R1_C03_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNTNNCCTNN NNNNTTTTNN TTANNNNNGA AGTTTTAAAT 61 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGGAA TTATTATTTA AACTGTTTGA 121 GGAAACGCAG ATCGTTTTCG AAGAATGAAC GCAGGTCGGT GACGCCGTAA CGCAACATAG 181 TCAGACGCTC CATCCCCATC CCGAACCCGA AACCAGAGTA AACTTCCGGG TCGATGCCAA 241 CGTTACGCAA CACGTTCGGA TGCACCATCC CGCAGCCCAG CACTTCCAGC CATTTACCGT 301 TTTTACCCAT GACGTCCACT TCTGCAGAAG GTTCAGCAAA CGGGAAGTAG GAAGGACGGA 361 AGCGAATCTG CAAATCTTCC TCAAAGAAGT TACGCAGGAA GTCGTGCAGC GTGCCTTTCA 421 GGTTGGTAAA GCTGATGTTG GTATCAACAA TCAGACCTTC CATCTGATGG AACATCGGCG 481 TGTGAGTCTG GTCGTAGTCG TTACGATAAA CACGGCCAGG CGCGATGATA CGAATCGGTG 541 GCTGCTGGGC TTTCATGGTG CGGATCTGTA CGCCAGAGGT CTGGGTACGC AGCAGGCGGG 601 TAGTGTCAAA CCAGAAAGTG TCGTGGTCAG CGCGCGCCGG GTGGTGACCA GGAATGTTCA 661 GAGCATCGAA GTTATGATAA TCGTCTTCGA TTTCCGGCCC GGTTGCCACG GTAAAGCCAA 721 GCTCACCGAA GAAACTTTCG ATACGGTCGA TGGTACGGGT AACCGGATGC AGACCGCCGT 781 TTTCNATGCG ACGANCTGGC AGAGAGACAT CNANNCGTTT CCNCCGCCAG ANGCGCATTC 841 AGTGCAGCGC TTTCCAGTTC CGCTTTACNC GCATTCAGCG NCNGCTGANN CTGCTCTTTC 901 NCTNCNTTGA TAACCGCACN NGCTGCNNNA NGCTCNNCTN NNNNNAGCTC ACGCANGNNN 961 GTCNTCTGNN NGGNNNANTG NCCTTTTTTA NNNNNNNNTN NNNNNGNNNA TNNTCTNNNG 1021 NNGCANCNTC NNNNNNNNNN // |
Primer: SV40pA3'Element Reverse Region: ColE1 ori, MCS Sequence file: RDB17947_B0Bcd.seq ![]() |
>17947_17947_B0Bc_1_SV40pA3'elementReverse_G01_07_ABI08.ab1 1 NNNNNNNNNN NGNNNNNNNN NNNNNNNNNA NGCNNNNNNN NCGGCCTTTT TACGGTTCCT 61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 241 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 301 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 361 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 421 CACATATGAC CATGATTACG AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCGACCT 481 GCAGGCATGC AAGCTTGGCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG 541 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG 601 AAGANGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAN CCTGNATGGC NANNGGNGCC 661 TGATGCGGNA TTTTCTCNTT ANNCATCTGN GCNNNATTTC ACANCGCANN TGGNGCNNTC 721 TCNNNNCAAT CTGCTCNNNN NNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032. |
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2021.01.06
GNP_filter3_RDBDEP_html_201222.pl