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pSAL7A35VS

Bacterial expression vector for the salicylate-inducible high expression system in E. coli.

Catalog number RDB17946
Resource name pSAL7A35VS
Clone info. Bacterial expression vector for the salicylate-inducible high expression system in E. coli, see Fig. 1 of Miyazaki, K. PLoS One, 13 (4): e0194090, 2018 for constructs.
GFP fluorecence upon induction with 1 mM salicylic acid.
Vector backbone p18GFP (plasmid)
Selectable markers Ampicillin
Gene/insert name Uncultured bacterium (environmental sample) salicylate-responsive transcription factor genomic DNA
Depositor|Developer Miyazaki, Kentaro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Miyazaki K. et al. PLoS One, 13 (4): e0194090, 2018)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17946 pSAL7A35VS Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB17946zzk01.seq

Original reference

original Miyazaki, K., Molecular engineering of the salicylate-inducible transcription factor Sal7AR for orthogonal and high gene expression in Escherichia coli. PLoS One 13 (4): e0194090 (2018). PMID 29641575.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Bacteria host strains (en) (English text)
Featured content Bacteria host strains (ja) (Japanese text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Miyazaki, K., Molecular engineering of the salicylate-inducible transcription factor Sal7AR for orthogonal and high gene expression in Escherichia coli. PLoS One 13 (4): e0194090 (2018). PMID 29641575.

2020.10.26

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