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pSAL7A35VS

Bacterial expression vector for the salicylate-inducible high expression system in E. coli.

Catalog number RDB17946
Resource name pSAL7A35VS
Clone info. Bacterial expression vector for the salicylate-inducible high expression system in E. coli, see Fig. 1 of Miyazaki, K. PLoS One, 13 (4): e0194090, 2018 for constructs.
GFP fluorecence upon induction with 1 mM salicylic acid.
Vector backbone p18GFP (plasmid)
Selectable markers Ampicillin
Gene/insert name Uncultured bacterium (environmental sample) salicylate-responsive transcription factor genomic DNA
Depositor|Developer Miyazaki, Kentaro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Miyazaki K. et al. PLoS One, 13 (4): e0194090, 2018)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
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RDB17946 pSAL7A35VS DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB17946zzk01.seq

Original reference

original Miyazaki, K., Molecular engineering of the salicylate-inducible transcription factor Sal7AR for orthogonal and high gene expression in Escherichia coli. PLoS One 13 (4): e0194090 (2018). PMID 29641575.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Bacteria host strains (en) (English text)
Featured content Bacteria host strains (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17946_B0Bcp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: insert 5' (LTTR 5')
Sequence file: RDB17946_B0Bca.seq check
>17946_B1AkF_1_lacIpro-B_F_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNCGCNNC GNNTCNGTGA GCGAGGAAGC GGAANANCGC CCAATACGCA
   61 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG
  121 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC
  181 CCCAGGCTCA CACTGTGGTA GGGGCATCGG CGAACAGCTC AGATAGCAAC TGCCTGAGCC
  241 ACAGGTTGGC TGGATCACGG TTGTACTTGG CATGCCAGAA CAGGTTGATG GCGATTTCTG
  301 GCAGCGGGAT CGGATGAGGC GAGACGCTCA GTCCGAAGGG GTCCCTGCAG CGTTCGGCAA
  361 ACCGCTCCGG CAGCGTCGCA ATCAGGTCGG TGGATTGCAG GATATGCCCA ACAGCGATGA
  421 AGTGGGGTAC GACCAGCCGC ATGCGGCGCT TGACGCCGGC CCGTTCCAGC AGCTCGTCTA
  481 TTTCGGCGTG GCCGGTGTTG GCGGCCATCA CCCCGACATG CTCGAGCTCG CAGAATTGCT
  541 CTAGGCTCAT GGGCGTGGTG GCCGTCGCGT GCTGCTGGCG AAATACACAG ACATAGCGGT
  601 GCCGGAACAG GCGACGCTGG AAGAAGCCAG CCTGCAGGTT GGGCTGGTGG CCCAATGTCA
  661 GGTCGATTGA CCCCGACTCC ATGTCATCCC TGAGATTGCC GCTGTTGGGT CGCAGCGTGG
  721 TGATCCGGAN GCTGGGCGCG AGCCGGGCCA AGGCTTCCAT GAGCGGGGGC ATGAAGTACA
  781 TCTCGCCNAT GTCGGTCATG GCGAGGTTGA AGTTCCGGCT GCTGGTCAGC GGATCGAANG
  841 TGTCGCGCCG CGCCNGGANG GTCTGTANCG CATTGAGGGC ATACATCACC GGCTCCNANA
  901 GGNGCAGGGC ATANGGGGNC GGNNNCNTGC CACGCGAGGN GCGCANGNNN AGCTCGTCCT
  961 TGANTANCGN GCNCANCCGC TTGANGNNAT TGNNNANNNC GGGCTGGNNC NNNCCCNNCN
1021 NGNCANCNGN NNNNNNNNN
//
Primer: GFP-N_R
Region: insert mid (GFP 5', ORFx, LTTR 3')
Sequence file: RDB17946_B0Bcb.seq check
>17946_B1AkF_1_GFP-N_R_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNTCCNNNNN NNANTTCTTC TCCTTTGCTA GCCNTATGTA TATCTCCTTC
   61 TTAATTAATT AGGGGGTTCT ACGACGAGTT CCATATTTTT TTACCGTTGT CTCCGTTGGG
  121 TTGCGATTCT AAGAAGCCTT GCGATATAAG TCGATAAACA GCAACTGATA CACACAATCA
  181 CCAATGTGAA TAAAGGATGG CTTCATGGAT CTGCGCGACA TCGACCTCAA CCTGCTGGTG
  241 GTCTTCAATC AACTGCTGCT CGACCGCAGC GTCTCGACGG CCGCTGACAA GCTGGGCCTG
  301 ACCCAGCCCG CGGTCAGCAA TGCCCTCAAG CGGCTGCGCA CGGTACTCAA GGACGAGCTG
  361 TTCCTGCGCA CCTCGCGTGG CATGGAGCCG ACCCCCTATG CCCTGCACCT GTCGGAGCCG
  421 GTGATGTATG CCCTCAATGC GCTACAGACC GTCCTGGCGC GGCGCGACAC CTTCGATCCG
  481 CTGACCAGCA GCCGGAACTT CAACCTCGCC ATGACCGACA TTGGCGAGAT GTACTTCATG
  541 CCCCCGCTCA TGGAAGCCTT GGCCCGGCTC GCGCCCAGCA TCCGGATCAC CACGCTGCGA
  601 CCCAACAGCG GCAATCTCAG GGATGACATG GAGTCGGGGT CAATCGACCT GACATTGGGC
  661 CACCAGCCCA ACCTGCAGGC TGGCTTCTTC CAGCGTCGCC TGTTCCGGCA CCGCTATGTC
  721 TGTGTATTTC GCCAGCAGCA CGCGACGGCC ACCACGCCCA TGAGCCTAGA GCAATTCTGC
  781 GAGCTCGAGC ATGTCGGGGT GANGGNCGCC AACACCGGCC ACGCCGAAAT AGACGANCTG
  841 CTGGAACGGG CCGGCGTCNA GCGCCGCATG CGGCTGGTCG NACCCNACTT CATCGCTGTT
  901 GGNCATATNC TGCAATCCAC CNANCTGNNT GCGACGCTGC CNGANCGGTT TGNCNAACGC
  961 TGCNNNNNCC CTTNNNANTG ANCGTNNCGN CNNNNTCCNA TNCNNNNGCC NNNNNCNNCN
1021 NTNNNNCNNG NTNNNNNNNN NN
//
Primer: HB-NOS_R
Region: insert 3' (GFP 3')
Sequence file: RDB17946_B0Bcc.seq check
>17946_B1AkF_1_HB-NOS_R_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNNNN NTGGNNNNNN CNGGGTTTTC CCNGTCACGA CGTTGTAAAA
   61 CGACGGCCAG TGCCAAGCTT GCATGCTTAG TATAGTTCAT CCATGCCATG TGTAATCCCA
  121 GCAGCTGTTA CAAACTCAAG AAGGACCATG TGGTCTCTCT TTTCGTTGGG ATCTTTCGAA
  181 AGGGCAGATT GTGTGGACAG GTAATGGTTG TCTGGTAAAA GGACAGGGCC ATCGCCAATT
  241 GGAGTATTTT GTTGATAATG GTCTGCTAGT TGAACGCTTC CATCTTCAAT GTTGTGTCTA
  301 ATTTTGAAGT TAGCTTTGAT TCCATTCTTT TGTTTGTCTG CAGTGATGTA TACATTGTGT
  361 GAGTTATAGT TGTATTCCAA TTTGTGTCCA AGAATGTTTC CATCTTCTTT AAAATCAATA
  421 CCTTTTAACT CGATTCTATT AACAAGGGTA TCACCTTCAA ACTTGACTTC AGCACGTGTC
  481 TTGTAGTTCC CGTCATCTTT GAAGCTTATA GTTCTTTCCT GTACATAACC TTCGGGCATG
  541 GCACTCTTGA AAAAGTCATG CCGTTTCATA TGATCCGGGT ATCTTGAAAA GCATTGAACA
  601 CCATAAGTCA GAGTAGTGAC AAGTGTTGGC CATGGAACAG GTAGTTTTCC AGTAGTGCAA
  661 ATAAATTTAA GGGTAAGTTT TCCGTATGTT GCATCACCTT CACCCTCTCC ACTGACAGAA
  721 AATTTGTGCC CATTAACATC ACCATCTAAT TCAACAAGAA NTGGGACAAC TCCNGTGAAG
  781 AGTTCTTCTC CTTTGCTAGC CATATGTATA TCTCCTTCTT AATTAATTAN GGGGTTCTAC
  841 GACGAGTTCC ATATTTTTTT ACNGTTGTCT CCGTTGGGTT GCNANTCTAA NAANCCTTGC
  901 NATATAANTC GATAAACAGC AACTGANACN CACNATCACN NATGTGAATA AAGNNNGGNT
  961 TCNTGGNNCT GNNCGANNTC NANCNNNNNC NGCTGGNGGN CNTCNNTCNN CTGCTGNNNG
1021 ANNNNANCGT CNCNANNNNN NNTGANNNNN NGGNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Miyazaki, K., Molecular engineering of the salicylate-inducible transcription factor Sal7AR for orthogonal and high gene expression in Escherichia coli. PLoS One 13 (4): e0194090 (2018). PMID 29641575.

2021.02.01

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