Resource data sheet
DNA Bank Top

pSAL7A35VS (#RDB17946)

Bacterial expression vector for the salicylate-inducible high expression system in E. coli.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Miyazaki, K., PLoS One 13 (4): e0194090 (2018). PMID 29641575. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Bacterial expression vector for the salicylate-inducible high expression system in E. coli, see Fig. 1 of Miyazaki, K. PLoS One, 13 (4): e0194090, 2018 for constructs.
Comment GFP fluorecence upon induction with 1 mM salicylic acid.
Vector backbone p18GFP (plasmid)
Selectable markers Ampicillin
Growth conditions LB+Ampicillin, 37oC
Gene/insert name Uncultured bacterium (environmental sample) salicylate-responsive transcription factor genomic DNA
Depositor|Developer Miyazaki, Kentaro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Miyazaki K. et al. PLoS One, 13 (4): e0194090, 2018)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyazaki K. et al. PLoS One, 13 (4): e0194090, 2018)。

Catalog # Resource name Shipping form Fee
RDB17946 pSAL7A35VS DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pSAL7A35VS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17946).

Reference section:

Miyazaki, K., Molecular engineering of the salicylate-inducible transcription factor Sal7AR for orthogonal and high gene expression in Escherichia coli. PLoS One 13 (4): e0194090 (2018). PMID 29641575. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Miyazaki, K., Molecular engineering of the salicylate-inducible transcription factor Sal7AR for orthogonal and high gene expression in Escherichia coli. PLoS One 13 (4): e0194090 (2018). PMID 29641575. [PubMed] [Article] [RRC of NBRP]