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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSAL7A35VS

Bacterial expression vector for the salicylate-inducible high expression system in E. coli.

Catalog number RDB17946
Resource name pSAL7A35VS
Clone info. Bacterial expression vector for the salicylate-inducible high expression system in E. coli, see Fig. 1 of Miyazaki, K. PLoS One, 13 (4): e0194090, 2018 for constructs.
Comment GFP fluorecence upon induction with 1 mM salicylic acid.
Vector backbone p18GFP (plasmid)
Selectable markers Ampicillin
Gene/insert name Uncultured bacterium (environmental sample) salicylate-responsive transcription factor genomic DNA
Depositor|Developer Miyazaki, Kentaro |
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External Database
Uncultured bacterium (environmental sample) salicylate-responsive transcription factor

            Reference sequence
              
             
            Sequence (full) RDB17946zzk01.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Miyazaki K. et al. PLoS One, 13 (4): e0194090, 2018)
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyazaki K. et al. PLoS One, 13 (4): e0194090, 2018)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB17946 pSAL7A35VS DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Miyazaki, K., Molecular engineering of the salicylate-inducible transcription factor Sal7AR for orthogonal and high gene expression in Escherichia coli. PLoS One 13 (4): e0194090 (2018). PMID 29641575.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Bacteria host strains (en) (English text)
            Featured content Bacteria host strains (ja) (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17946_B0Bcp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: insert 5' (LTTR 5')
            Sequence file: RDB17946_B0Bca.seq check
            >17946_B1AkF_1_lacIpro-B_F_E01_13_ABI24.ab1
                1 NNNNNNNNNN NNNNCGCNNC GNNTCNGTGA GCGAGGAAGC GGAANANCGC CCAATACGCA
               61 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG
              121 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC
              181 CCCAGGCTCA CACTGTGGTA GGGGCATCGG CGAACAGCTC AGATAGCAAC TGCCTGAGCC
              241 ACAGGTTGGC TGGATCACGG TTGTACTTGG CATGCCAGAA CAGGTTGATG GCGATTTCTG
              301 GCAGCGGGAT CGGATGAGGC GAGACGCTCA GTCCGAAGGG GTCCCTGCAG CGTTCGGCAA
              361 ACCGCTCCGG CAGCGTCGCA ATCAGGTCGG TGGATTGCAG GATATGCCCA ACAGCGATGA
              421 AGTGGGGTAC GACCAGCCGC ATGCGGCGCT TGACGCCGGC CCGTTCCAGC AGCTCGTCTA
              481 TTTCGGCGTG GCCGGTGTTG GCGGCCATCA CCCCGACATG CTCGAGCTCG CAGAATTGCT
              541 CTAGGCTCAT GGGCGTGGTG GCCGTCGCGT GCTGCTGGCG AAATACACAG ACATAGCGGT
              601 GCCGGAACAG GCGACGCTGG AAGAAGCCAG CCTGCAGGTT GGGCTGGTGG CCCAATGTCA
              661 GGTCGATTGA CCCCGACTCC ATGTCATCCC TGAGATTGCC GCTGTTGGGT CGCAGCGTGG
              721 TGATCCGGAN GCTGGGCGCG AGCCGGGCCA AGGCTTCCAT GAGCGGGGGC ATGAAGTACA
              781 TCTCGCCNAT GTCGGTCATG GCGAGGTTGA AGTTCCGGCT GCTGGTCAGC GGATCGAANG
              841 TGTCGCGCCG CGCCNGGANG GTCTGTANCG CATTGAGGGC ATACATCACC GGCTCCNANA
              901 GGNGCAGGGC ATANGGGGNC GGNNNCNTGC CACGCGAGGN GCGCANGNNN AGCTCGTCCT
              961 TGANTANCGN GCNCANCCGC TTGANGNNAT TGNNNANNNC GGGCTGGNNC NNNCCCNNCN
            1021 NGNCANCNGN NNNNNNNNN
            //
            Primer: GFP-N_R
            Region: insert mid (GFP 5', ORFx, LTTR 3')
            Sequence file: RDB17946_B0Bcb.seq check
            >17946_B1AkF_1_GFP-N_R_F01_16_ABI24.ab1
                1 NNNNNNNNNN NNTCCNNNNN NNANTTCTTC TCCTTTGCTA GCCNTATGTA TATCTCCTTC
               61 TTAATTAATT AGGGGGTTCT ACGACGAGTT CCATATTTTT TTACCGTTGT CTCCGTTGGG
              121 TTGCGATTCT AAGAAGCCTT GCGATATAAG TCGATAAACA GCAACTGATA CACACAATCA
              181 CCAATGTGAA TAAAGGATGG CTTCATGGAT CTGCGCGACA TCGACCTCAA CCTGCTGGTG
              241 GTCTTCAATC AACTGCTGCT CGACCGCAGC GTCTCGACGG CCGCTGACAA GCTGGGCCTG
              301 ACCCAGCCCG CGGTCAGCAA TGCCCTCAAG CGGCTGCGCA CGGTACTCAA GGACGAGCTG
              361 TTCCTGCGCA CCTCGCGTGG CATGGAGCCG ACCCCCTATG CCCTGCACCT GTCGGAGCCG
              421 GTGATGTATG CCCTCAATGC GCTACAGACC GTCCTGGCGC GGCGCGACAC CTTCGATCCG
              481 CTGACCAGCA GCCGGAACTT CAACCTCGCC ATGACCGACA TTGGCGAGAT GTACTTCATG
              541 CCCCCGCTCA TGGAAGCCTT GGCCCGGCTC GCGCCCAGCA TCCGGATCAC CACGCTGCGA
              601 CCCAACAGCG GCAATCTCAG GGATGACATG GAGTCGGGGT CAATCGACCT GACATTGGGC
              661 CACCAGCCCA ACCTGCAGGC TGGCTTCTTC CAGCGTCGCC TGTTCCGGCA CCGCTATGTC
              721 TGTGTATTTC GCCAGCAGCA CGCGACGGCC ACCACGCCCA TGAGCCTAGA GCAATTCTGC
              781 GAGCTCGAGC ATGTCGGGGT GANGGNCGCC AACACCGGCC ACGCCGAAAT AGACGANCTG
              841 CTGGAACGGG CCGGCGTCNA GCGCCGCATG CGGCTGGTCG NACCCNACTT CATCGCTGTT
              901 GGNCATATNC TGCAATCCAC CNANCTGNNT GCGACGCTGC CNGANCGGTT TGNCNAACGC
              961 TGCNNNNNCC CTTNNNANTG ANCGTNNCGN CNNNNTCCNA TNCNNNNGCC NNNNNCNNCN
            1021 NTNNNNCNNG NTNNNNNNNN NN
            //
            Primer: HB-NOS_R
            Region: insert 3' (GFP 3')
            Sequence file: RDB17946_B0Bcc.seq check
            >17946_B1AkF_1_HB-NOS_R_G01_19_ABI24.ab1
                1 NNNNNNNNNN NNNNGNNNNN NTGGNNNNNN CNGGGTTTTC CCNGTCACGA CGTTGTAAAA
               61 CGACGGCCAG TGCCAAGCTT GCATGCTTAG TATAGTTCAT CCATGCCATG TGTAATCCCA
              121 GCAGCTGTTA CAAACTCAAG AAGGACCATG TGGTCTCTCT TTTCGTTGGG ATCTTTCGAA
              181 AGGGCAGATT GTGTGGACAG GTAATGGTTG TCTGGTAAAA GGACAGGGCC ATCGCCAATT
              241 GGAGTATTTT GTTGATAATG GTCTGCTAGT TGAACGCTTC CATCTTCAAT GTTGTGTCTA
              301 ATTTTGAAGT TAGCTTTGAT TCCATTCTTT TGTTTGTCTG CAGTGATGTA TACATTGTGT
              361 GAGTTATAGT TGTATTCCAA TTTGTGTCCA AGAATGTTTC CATCTTCTTT AAAATCAATA
              421 CCTTTTAACT CGATTCTATT AACAAGGGTA TCACCTTCAA ACTTGACTTC AGCACGTGTC
              481 TTGTAGTTCC CGTCATCTTT GAAGCTTATA GTTCTTTCCT GTACATAACC TTCGGGCATG
              541 GCACTCTTGA AAAAGTCATG CCGTTTCATA TGATCCGGGT ATCTTGAAAA GCATTGAACA
              601 CCATAAGTCA GAGTAGTGAC AAGTGTTGGC CATGGAACAG GTAGTTTTCC AGTAGTGCAA
              661 ATAAATTTAA GGGTAAGTTT TCCGTATGTT GCATCACCTT CACCCTCTCC ACTGACAGAA
              721 AATTTGTGCC CATTAACATC ACCATCTAAT TCAACAAGAA NTGGGACAAC TCCNGTGAAG
              781 AGTTCTTCTC CTTTGCTAGC CATATGTATA TCTCCTTCTT AATTAATTAN GGGGTTCTAC
              841 GACGAGTTCC ATATTTTTTT ACNGTTGTCT CCGTTGGGTT GCNANTCTAA NAANCCTTGC
              901 NATATAANTC GATAAACAGC AACTGANACN CACNATCACN NATGTGAATA AAGNNNGGNT
              961 TCNTGGNNCT GNNCGANNTC NANCNNNNNC NGCTGGNGGN CNTCNNTCNN CTGCTGNNNG
            1021 ANNNNANCGT CNCNANNNNN NNTGANNNNN NGGNNNNNNN NNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Miyazaki, K., Molecular engineering of the salicylate-inducible transcription factor Sal7AR for orthogonal and high gene expression in Escherichia coli. PLoS One 13 (4): e0194090 (2018). PMID 29641575.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl