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pUC4KrA1Hg

Plasmid clone of T. thermophilus strain HB27 16S ribosomal RNA gene (rrsA) and thermostable hygromycin resistant gene for gene displacement.

Catalog number RDB17945
Resource name pUC4KrA1Hg
Clone info. Plasmid clone of T. thermophilus strain HB27 16S ribosomal RNA gene (rrsA) and thermostable hygromycin resistant gene (HgR) for gene displacement experiments.
Vector backbone pUC18 (plasmid)
Selectable markers Ampicillin
Gene/insert name Thermus thermophilus HB27 rrsA genomic DNA E. coli hygromycin phosphotransferase genomic DNA
Depositor|Developer Miyazaki, Kentaro |
 
Other clones in our bank Thermus thermophilus HB27 (KEGG orthology K01977) | E. coli (KEGG orthology K18817) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Miyazaki K. et al. Sci. Rep. 9 (1): 11233, 2019)
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RDB17945 pUC4KrA1Hg Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB17945zzk01.seq

Original reference

original Miyazaki, K., Occurrence of randomly recombined functional 16S rRNA genes in Thermus thermophilus suggests genetic interoperability and promiscuity of bacterial 16S rRNAs. Sci. Rep. 9 (1): 11233 (2019). PMID 31375780.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Miyazaki, K., Occurrence of randomly recombined functional 16S rRNA genes in Thermus thermophilus suggests genetic interoperability and promiscuity of bacterial 16S rRNAs. Sci. Rep. 9 (1): 11233 (2019). PMID 31375780.

2020.10.26

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