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rabbit MACS1-luc2 (#RDB17899)

Luciferase reporter of the rabbit Shh enhancer, MACS1.

Clone info. Luciferase reporter of the rabbit Sonic hedgehog (Shh) enhancer, MACS1.
Comment Expression was confirmed by the depositor with luciferase assay.
Vector backbone pGL4.23 (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Ampicillin
Gene/insert name rabbit SHH genomic
Depositor|Developer Amano, Takanori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17899 rabbit MACS1-luc2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The rabbit MACS1-luc2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17899).

Reference section:

Tsukiji, N., Amano, T., Shiroishi, T., A novel regulatory element for Shh expression in the lung and gut of mouse embryos. Mech. Dev. 131: 127-136 (2014). PMID 24157522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17899_B0Afp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665)
Region: Pause site , rabbit MACS1 5'
Sequence file: RDB17899_B0Afa.seq check
>D06932A4_B0Af_1_pGL4ins_F_B11_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN NNAGGCTGTC CCCNGTGCAA GTGCAGGTGC CAGAACATTT
   61 CTCTGGCCTA ACTGGCCGGT ACCTGAGCTC GCTAGCCTCG AGATTCCCTC CCTTTTTATG
  121 TGATGGACAC CAGATGACCT GAGTAACGGC AGGGCAAGAC CCAGGAGAAA CAAATGACAA
  181 CCGAGCTCTC GTTTTTAAAC TAATGGTTAA TACCACGAAT CCTACAGAAA CAGACGGAAA
  241 TAAAATTTCC AATTTGAAAA AAGAGCAAGT GTCACTGTAT TTCTCTCTTA AGTCTCCTGT
  301 TTCAGACACC TGATGAATTA AAGTAACTTG AGAGTCTGGG CTATTGATAT GTAAAGCGAT
  361 TACTGGTCAT TTACAAGCAA CATACTGGCT AACCATTAAA AAAAAATACT AACAGAAGTA
  421 CCCAGGTGAA CTTTGTCTAC CCAAGAGGCC TCTGTGAGTG ATCCATCTGG ATGTCAGCAA
  481 ATTGCTTACT AATGTGTTGA CTTATGGCTG CCCTCAAAAG CAAAATGAAA AGCCTGGAAT
  541 TTACAGCATA TCAATGAATC AGTTGATAAG CTTTTACTGG TTTAATATCC TTCCAGTCTT
  601 GCTGCTTGGA GCCACTTCAA GTTAGCAACA GAATGTTTGC CATCTCCACG TGATAAAGGA
  661 AGTCAAAGGA CTAAACAAAC TTTCAGACCA TTATGCTGTT TGGTGAGATG TAAATGAAAA
  721 AAGTAAGAGA AACCCCAGGC CTGANGGTGT GGGANCGGGC AGCCCCTANG CTGACAAATC
  781 ACTAACCTCA CTTTTNAAAC AACGGCCAGG ANTTTTAGCT GGGGTGGAGA TAATATAGTG
  841 CATTTGAAAA GTTTTNATTG CAGCAATAAA AGANCATACC TCAATTATAT GACATATGCT
  901 AGCAGAATTT TNANATAANG ANNAAAGGNN NTCNAGGANC NAGCACNGCA TTCTGGGNNA
  961 CACTNNGGCT TTCTGNACNC NCNNGNNNNN GNNANNANCA TNNNAGCNNN GNNGNNNNTT
1021 CNNNNNNNNT NNNNNNNNNT TNNNNNAANC NNNNNNNNNN
//
Primer: rabbit MACS1_F1 (Pr0812)
Region: rabbit MACS1 3'
Sequence file: RDB17899_B0Afb.seq check
>D06932A4_B0Af_1_rabbit MACS_F1_H08_08_ABI08.ab1
    1 NNNNNNNNNN NNNNGNNNNG NAANNGAAAA NNNNAAGAGA AACCCCAGGC CTGAGGGTGT
   61 GGGAGCGGGC AGCCCCTAGG CTGACAAATC ACTAACCTCA CTTTTTAAAC AACGGCCAGG
  121 ACTTTTAGCT GGGGTGGAGA TAATATAGTG CATTTGAAAA GTTTTAATTG CAGCAATAAA
  181 AGACCATACC TCAATTATAT GACATATGCT AGCAGAATTT TTAGATAAGG ATCAAAGGCA
  241 TTCAAGGACC AAGCACTGCA TTCTGGGCAA CACTTGGGCT TTCTGTACAC ACTGGTGCCG
  301 GGTATTAGCA TAACAGCTCT GCTGACCTTT CAGGGCCTCT TTGATCTGAG TTTGTCAAAA
  361 CCAGAATGAA TGTGCACTGG GACTACTGTA TCATCCCCAG ACAAGTCAAA TACCCCCTGT
  421 GACAAAGGCC TTGCTGACTG CATTTTCATT CTCTGACACT CACTCAAGGG GACACAACCC
  481 AACATCTGAC CTCTCAGTTC TTGCCACAAG ACTGGCATAT GGAAAAGAAC CTCGTTTGTA
  541 ACCTGACTTC GCTTTGTGAG GATACGTACG CCAGGACTTT AAAGCAAGGC ACCTTTGTGA
  601 AAAGAAACCC TCCATGTATG GAAGTAGTCC TATTTGTAGA AAGATGATCC CTGCGTTTCA
  661 TTAATTAGTT AACCCCCTAG TAGATGCGTG AAAAACAAAG ATCTGGCCTC GGCGGCCAAG
  721 CTTAGACACT AGAGGGTATA TAATGGAAGC TCGACTTCCA GCTTGGCAAT CCGGTACTGT
  781 TGGTAAAGCC ACCATGGAAG ANGCCNAAAA CATTAAGAAN GGCCCAGCGN CNTTCTACCN
  841 ACTCGAANAN NGGNNCNCCN GCGANCAGCT GCNCNAAGCC NTGAAGCGNT ACGCCCNNNN
  901 NCCNGGNNCC NTCGCCNTNN NNGACGNNCA TATCGAGGNN NNNNTNACNN ACNNNNNNNN
961 CTTNNNNATN ANCNNNNNNN NNGNNNNNNN NNN
//
Primer: Luc_R (Pr0146)
Region: luc2 5', mini P, rabbit MACS1 3'
Sequence file: RDB17899_B0Afc.seq check
>D06932A4_B0Af_1_Luc_R_C11_08_ABI24.ab1
    1 NNNNNNNNNN GNNNNNNNNN NNGNANCGCT TCNTGGCTTT GTGCNGCTGC TCGCCGGCGG
   61 TCCCGTCTTC GAGTGGGTAG AATGGCGCTG GGCCCTTCTT AATGTTTTTG GCATCTTCCA
  121 TGGTGGCTTT ACCAACAGTA CCGGATTGCC AAGCTGGAAG TCGAGCTTCC ATTATATACC
  181 CTCTAGTGTC TAAGCTTGGC CGCCGAGGCC AGATCTTTGT TTTTCACGCA TCTACTAGGG
  241 GGTTAACTAA TTAATGAAAC GCAGGGATCA TCTTTCTACA AATAGGACTA CTTCCATACA
  301 TGGAGGGTTT CTTTTCACAA AGGTGCCTTG CTTTAAAGTC CTGGCGTACG TATCCTCACA
  361 AAGCGAAGTC AGGTTACAAA CGAGGTTCTT TTCCATATGC CAGTCTTGTG GCAAGAACTG
  421 AGAGGTCAGA TGTTGGGTTG TGTCCCCTTG AGTGAGTGTC AGAGAATGAA AATGCAGTCA
  481 GCAAGGCCTT TGTCACAGGG GGTATTTGAC TTGTCTGGGG ATGATACAGT AGTCCCAGTG
  541 CACATTCATT CTGGTTTTGA CAAACTCAGA TCAAAGAGGC CCTGAAAGGT CAGCANANCT
  601 GTTATGCTAA TACCCGGCAC CAGTGTGTAC AGAAAGCCCA AGTGTTGNCN ANAANGCAGN
  661 GNTNGGTCCT TGAATGNCTT TGATCCNTAT CTNAAAATTC TGNNNNNNNA TGTNNNATAA
721 TTGAGGNNNN GNNNNTTNNN NGNNNNNNTT NAAANTTTTC NAANGNNNNN NNNNN
//
Primer: pGL4pA_R (Pr0664)
Region: SV40 late p(A), luc2 3'
Sequence file: RDB17899_B0Afd.seq check
>D06932A4_B0Af_1_pGL4pA_R_D11_11_ABI24.ab1
    1 NNNNNNNNNN NNGGNNNNTT ATCGANTTTN CNNNNTTTGT AGAGGTTTTA CTTGCTTTAA
   61 AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA
  121 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA
  181 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT
  241 ATCATGTCTG CTCGAAGCGG CCGGCCGCCC CGACTCTAGA ATTATTACAC GGCGATCTTG
  301 CCGCCCTTCT TGGCCTTAAT GAGAATCTCG CGGATCTTGC GGGCGTCCAA CTTGCCGGTC
  361 AGTCCTTTAG GCACCTCGTC CACGAACACA ACACCACCGC GCAGCTTCTT GGCGGTTGTA
  421 ACCTGGCTGG CCACATAGTC CACGATCTCC TTCTCGGTCA TGGTTTTACC GTGTTCCAGC
  481 ACGACGACNG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC GACCCCGGCG
  541 TCGAAGATGT TGGGGTGTTG CAGCAGGATG CTCTCCAGTT CGGCTGGGGC TACCTGGTAG
  601 CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC GTCCTCGTCC
  661 CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC GTTTGTAGCC
  721 TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG CTCGCCGCGC
  781 TGGTTCACAC CCAGTGTCTT ACCGGTGTCC NAGTCCACCA CCTTANCCTC NAAGAANGGC
  841 ACCACCTTGC CTACTGCGCC AGGCTNGTCG TCCCCTTCNG GGGTGANCAG AANGGCGCTG
  901 GTTGNTTCTG TCNNGNCGTA NCCCNGGCGG ANGNCNGGNN NNGGAANCGN TNNNNCNCGG
  961 NCTCNCCNNC CNNCTTGCTG ANCGGNGCCN NNNNNCTGGN NATNNNGNGN NANTNGNNNN
1021 NNNNNGNANN NNNNNATNAN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsukiji, N., A novel regulatory element for Shh expression in the lung and gut of mouse embryos. Mech. Dev. 131: 127-136 (2014). PMID 24157522. [link to RRC of NBRP]