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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

rat MACS1-luc2 (#RDB17898)

Luciferase reporter of the ACI/N rat Shh enhancer, MACS1.

Clone info. Luciferase reporter of the ACI/N rat Sonic hedgehog (Shh) enhancer, MACS1.
Comment Expression was confirmed by the depositor with luciferase assay.
Vector backbone pGL4.23 (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Ampicillin
Gene/insert name rat Shh genomic
Depositor|Developer Amano, Takanori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17898 rat MACS1-luc2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The rat MACS1-luc2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17898).

Reference section:

Tsukiji, N., Amano, T., Shiroishi, T., A novel regulatory element for Shh expression in the lung and gut of mouse embryos. Mech. Dev. 131: 127-136 (2014). PMID 24157522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17898_B0Afp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665)
Region: Pause site, rat MACS1 5'
Sequence file: RDB17898_B0Afa.seq check
>D06932A3_B0Af_2_pGL4ins_F_G10_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNNGC NNATAGGCTG TCCCCAGTGC AAGTGCAGGT GCCAGAACAT
   61 TTCTCTGGCC TAACTGGCCG GTACCTGAGC TCGCTAGCCT CGAGATAACC AGGAGTCCAG
  121 GTACTTGATA TGCAAAGCAA TACTAGTCAT TTGAAAACAG TACACTGGCT AAATGTTAAA
  181 AAAAAAAAAC AACAAAAGCA TTTAGGTGAA TTCTGTCTGC TCAGGAAAGT CTTAAGTGAT
  241 CCATCTCATG TCAGCAAATT GCTTACTAAT GTGTTGACTT AGGGCCACTC TCAAAAGCAA
  301 AAAGAAAAGC CTGGAATTTA TAGCATCTCA ATGCATCAGC TGATAAGCTT TTACTGGTTT
  361 AATATGCTTC CAGGCTTGCA GCCTGGAGCC ACTTCAAGTT AGCAACAGAA TGTTTGCCAT
  421 CTCCATGTGA TAAAGGAAGT CAAAGGGATG AACAAACTTC AGGACCATTA TGCTGTTTGG
  481 TGAGATGGAA ATGAAAAACT AGAGAAGTCA CAGGTTTGGG GGTGTGTGAG AGCTGGGCCT
  541 TCAAGTCCAG CATCTGACGG ATTGTTAGCC TCACTTCTCA AACAATGGTC TGGACTTTAG
  601 CTGGGGCAGA GATAACATAG TGCATTTGAA AAGTTTTAAT TGCAGCAATA AAAGACCATG
  661 CCTCAATTAT ATGACAGATG CTAGCAAAGT TTCCAGATAA GGATCAAAGC CATCCNAGGA
  721 GTGNNCACTG CTCTTTGGGC CACACTAGGC TTTCTGTACA CACTGCTGGG GAATTAGCAC
  781 AGCAGCTTTT CCNANCTTTT TTCTTCCTTT GATGTGAGTT CCTAAGCNAG TTAAGTGTAT
  841 CAAGACTACN GTATCAGCCA GANNGGTCCN ACACTCCNNT GCTGAATTTT AATCCTGANA
  901 CTCNACAGTA CAAAANCNAA TACNNGANNN TTGCAGTTCT TANCNCAAAA GTNCANNGNG
  961 NTANNTNNNG NNNNGGCGGN NNAGCTNNNA NNNNANANGG NANANNNNGN NNNNNNGANT
1021 NNNNNCNNNN NNNNNN
//
Primer: Luc_R (Pr0146)
Region: luc2 5', rat MACS1 3'
Sequence file: RDB17898_B0Afb.seq check
>D06932A3_B0Af_2_Luc_R_H10_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNANCGCTTN NTGGCTTTGT GCNGCTGCTC GCCGGCGGTC
   61 CCGTCTTCGA GTGGGTAGAA TGGCGCTGGG CCCTTCTTAA TGTTTTTGGC ATCTTCCATG
  121 GTGGCTTTAC CAACAGTACC GGATTGCCAA GCTGGAAGTC GAGCTTCCAT TATATACCCT
  181 CTAGTGTCTA AGCTTGGCCG CCGAGGCCAG ATCTAGCACA CTGAACTTTT GTGGTAAGAA
  241 CTGCAAAGGT CAGGTATTAG GTTTTGTACT GTTGAGTGTC AGGATTAAAA TTCAGCAAGG
  301 GAGTGTTGGA CCTGTCTGGC TGATACAGTA GTCTTGATAC ACTTAACTTG CTTAGGAACT
  361 CACATCAAAG GAAGAAAAAA GGTCGGAAAA GCTGCTGTGC TAATTCCCCA GCAGTGTGTA
  421 CAGAAAGCCT AGTGTGGCCC AAAGAGCAGT GGCCACTCCT TGGATGGCTT TGATCCTTAT
  481 CTGGAAACTT TGCTAGCATC TGTCATATAA TTGAGGCATG GTCTTTTATT GCTGCAATTA
  541 AAACTTTTCA AATGCACTAT GTTATCTCTG CCCCAGCTAA AGTCCAGACC ATTGTTTGAG
  601 AAGTGAGGCT AACAATCCGT CAGATGCTGG ACTTGAAGGC CCAGCTCTCA CACACCCCCA
  661 AACCTGTGAC TTCTCTAGTT TTTCATTTCC ATCTCACCAA ACAGCATAAT GGTCCTGAAG
  721 TTTGTTCATC CCTTTGACTT CCTTTATCAC ATGGAGATGG CAAACATTCT GTTGCTAACT
  781 TGAAGTGGCT CCNGGCTGCA AGCCTGGAAG CATATTANAC CAGTAAAAGC TTATCAGCTG
  841 ANGCATTGAG ATGCTATAAA TTCCAGGCTT TTCTTTTTGC TTTTGANANT GGNCCTAANN
  901 CNACACATTA NTAAGCAATT TGCTGANNTN AGANGNNTCA CTNNANANTT TCCTGANCAN
  961 ANAGANNNCA CCNAAANGCT TTNGNNGNTT TTTTTTTTTN NNNTTTANCN NGNNNNNNNN
1021 TTTNNAANNN NNNNNNN
//
Primer: pGL4pA_R (Pr0664)
Region: SV40 late p(A), luc2 3'
Sequence file: RDB17898_B0Afc.seq check
>D06932A3_B0Af_2_pGL4pA_R_A11_02_ABI24.ab1
    1 NNNNNNNNNN NNNGNNCCTT ATCGANTTNA CNNCNTTTGT AGAGGTTTTA CTTGCTTTAA
   61 AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA
  121 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA
  181 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT
  241 ATCATGTCTG CTCGAAGCGG CCGGCCGCCC CGACTCTAGA ATTATTACAC GGCGATCTTG
  301 CCGCCCTTCT TGGCCTTAAT GAGAATCTCG CGGATCTTGC GGGCGTCCAA CTTGCCGGTC
  361 AGTCCTTTAG GCACCTCGTC CACGAACACA ACACCACCGC GCAGCTTCTT GGCGGTTGTA
  421 ACCTGGCTGG CCACATAGTC CACGATCTCC TTCTCGGTCA TGGTTTTACC GTGTTCCAGC
  481 ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC GACCCCGGCG
  541 TCGAAGATGT TGGGGTGTTG CAGCAGGATG CTCTCCAGTT CGGCTGGGGC TACCTGGTAG
  601 CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC GTCCTCGTCC
  661 CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC GTTTGTAGCC
  721 TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG CTCGCCGCGC
  781 TGGTTCACAC CCAGTGTCTT ACCGGTGTCC AAGTCCACCA CCTTAGCCTC GAANAANGGC
  841 ACCACCTTGC CNACTGCGCC NGGCTNGTCG TCCCCTTCNG GGGTGANCAN ANNGGCGCTG
  901 GTTGTTNCTG TCNNGNCGNA NCCCNGGCGG ANGNCNNNNN NNNGGAAGCG NTNGGNCNCG
  961 GCCTCNCNNA CCTCCTTGCT GANCGGNGCC CNGNNNCNNG NGATNNNNNG NNNNTNGCTN
1021 NNNNNGNANN NNNNNNNGAN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsukiji, N., A novel regulatory element for Shh expression in the lung and gut of mouse embryos. Mech. Dev. 131: 127-136 (2014). PMID 24157522. [link to RRC of NBRP]