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pMal-c2X MBP-GFP1-10

Bacterial expression vector of GFP1-10 fused with soluble maltose-binding protein (MBP).

Catalog number RDB17883
Resource name pMal-c2X MBP-GFP1-10
Clone info. Bacterial expression vector of GFP1-10 fused with soluble maltose-binding protein (MBP) at N-terminus. GFP1-10 is expressed as a soluble fraction of E. coli by fusing with soluble MBP.
Comment MBP-GFP1-10 contains a TEV protease cleavage site between MBP and GFP1-10.Expression was confirmed by the depositor with Westernblotting using anti-His-tag antibody, Ni-affinity chromatography and SDS-PAGE.
Vector backbone pMal-c2X (Plasmid)
Size of vector backbone 6.6 kb
Selectable markers Ampicillin
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Inaba, Hiroshi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             
            Sequence (full) RDB17883zzk01.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Inaba H. et al., Chem. Commun(Camb). 55(62), 9072-9075, 2019) The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written approval from the DEPOSITOR using an Approval Form. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for an academic research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Approval form by depositor [Word]
            Remarks
            Please obtain written approval of the depositor.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inaba H. et al., Chem. Commun(Camb). 55(62), 9072-9075, 2019)。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            寄託者による提供承諾書 [Word]
            備考
            寄託者の書面による提供承諾が必要です。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB17883 pMal-c2X MBP-GFP1-10 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Original reference

            original Inaba, H., Stabilization of microtubules by encapsulation of the GFP using a Tau-derived peptide. Chem. Commun. (Camb) 55 (62): 9072-9075 (2019). PMID 31268098.

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Inaba, H., Stabilization of microtubules by encapsulation of the GFP using a Tau-derived peptide. Chem. Commun. (Camb) 55 (62): 9072-9075 (2019). PMID 31268098.

            2022.08.12

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