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FLAG-MAP1B-LC1 delta MAGD (#RDB17876)

Expression vector of human MAP1B-LC1 mutant, deletion of the mitochondrial aggregation and genome destruction domain (MAGD domain).

Clone info. Expression vector of human microtubule-associated protein 1B-light chain 1 (MAP1B-LC1) mutant, deletion of the mitochondrial aggregation and genome destruction domain (MAGD domain), tagged with FLAG at N-terminus.
Comment Expression was confirmed by the depositor with western blotting and immunoprecipitation.
Vector backbone pCMV5 (plasmid)
Selectable markers Ampicillin
Gene/insert name human MAP1B cDNA
Depositor|Developer Tagaya, Mitsuo |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Arasaki K. et al., EMBO Rep. 19 (8), e45584, 2018)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Arasaki K. et al., EMBO Rep. 19 (8), e45584, 2018)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17876 FLAG-MAP1B-LC1 delta MAGD Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The FLAG-MAP1B-LC1 delta MAGD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17876).

Reference section:

Yonashiro, R., Kimijima, Y., Shimura, T., Kawaguchi, K., Fukuda, T., Inatome, R., Yanagi, S., Mitochondrial ubiquitin ligase MITOL blocks S-nitrosylated MAP1B-light chain 1-mediated mitochondrial dysfunction and neuronal cell death. Proc. Natl. Acad. Sci. U.S.A. 109 (7): 2382-2387 (2012). PMID 22308378. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Yonashiro, R., Mitochondrial ubiquitin ligase MITOL blocks S-nitrosylated MAP1B-light chain 1-mediated mitochondrial dysfunction and neuronal cell death. Proc. Natl. Acad. Sci. U.S.A. 109 (7): 2382-2387 (2012). PMID 22308378. [link to RRC of NBRP]