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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

FLAG-MAP1B-LC1 Full (#RDB17873)

Expression vector of human MAP1B-LC1, full length.

Clone info. Expression vector of human microtubule-associated protein 1B-light chain 1 (MAP1B-LC1), full length (262 amino acid), tagged with FLAG at N-terminus.
Comment Expression was confirmed by the depositor with western blotting and immunoprecipitation.
Vector backbone pCMV5 (plasmid)
Selectable markers Ampicillin
Gene/insert name human MAP1B cDNA
Depositor|Developer Tagaya, Mitsuo |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Arasaki K. et al., EMBO Rep. 19 (8), e45584, 2018)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Arasaki K. et al., EMBO Rep. 19 (8), e45584, 2018)。

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RDB17873 FLAG-MAP1B-LC1 Full DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The FLAG-MAP1B-LC1 Full was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17873).

Reference section:

Yonashiro, R., Kimijima, Y., Shimura, T., Kawaguchi, K., Fukuda, T., Inatome, R., Yanagi, S., Mitochondrial ubiquitin ligase MITOL blocks S-nitrosylated MAP1B-light chain 1-mediated mitochondrial dysfunction and neuronal cell death. Proc. Natl. Acad. Sci. U.S.A. 109 (7): 2382-2387 (2012). PMID 22308378. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17873_A9L9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, FLAG, insert 5'
Sequence file: RDB17873_A9L9a.seq check
>D06426A1_A9L9_1_CMV_Forward_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNGCNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG AATTGATCTA CCATGGACTA CAAAGACGAT
  121 GACGACAAGC TTGCGGCCGC GAATTCGGAC CGCAGCCCTT CGCCACGCCA CCCTGATGTG
  181 TCCATGGTGG ACCCAGAGGC CTTGGCCATT GAGCAGAACC TGGGCAAAGC TCTAAAGAAA
  241 GATCTGAAAG AGAAGACCAA AACCAAAAAG CCAGGTACAA AGACCAAGTC ATCTTCACCT
  301 GTCAAAAAGA GTGATGGGAA GTCTAAGCCC TTGGCAGCTT CACCAAAACC AGCGGGCTTG
  361 AAAGAATCCT CGGATAAAGT GTCCAGGGTG GCTTCTCCTA AGAAGAAAGA ATCTGTGGAA
  421 AAGGCAGCAA AACCCACCAC CACTCCTGAG GTCAAAGCTG CACGTGGGGA AGAGAAAGAC
  481 AAGGAGACCA AGAATGCTGC CAATGCCTCT GCATCCAAGT CGGCCAAGAC CGCCACTGCA
  541 GGACCAGGAA CTACCAAGAC GACCAAGTCA TCTGCTGTGC CCCCAGGCCT CCCTGTGTAT
  601 TTGGACCTGT GCTACATTCC TAACCACAGC AATAGTAAGA ATGTTGATGT GGAATTTTTC
  661 AAGAGAGTGC GGTCTTCCTA CTACGTGGTG AGTGGGAATG ACCCTGCTGC TGAGGAGCCC
  721 AGCCGGGCTG TCCTGGACGC TTTGTTGGAA GGAAAGGNTC AGTGNGGCAG CAACATGCAG
  781 GTGACACTGA TCCCAACTCA TGACTCANAA GTGATGAGGG ANTGGTACCA GGAGANCCAT
  841 GANAAACAGC AAGATCTCAA CATCATGGTT TTANCAAGCA GCANCACAGN GGTTATGCAA
  901 NANGAATNCN TCCNNGCATG CNANANTGAN NTGNAAGTCN ANTCTNNANG ANCCNGGNNG
961 GNNTCCCNGN NNNCCNNNCC CANNGNNNNT NNNNNNNNNN
//
Primer: hGHpolyA-R (Pr0350)
Region: hGH p(A), insert 3'
Sequence file: RDB17873_A9L9b.seq check
>D06426A1_A9L9_1_hGHpolyA-R_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNANTA TAGAAGGANA CCTAGTCNGA CNAAATGATG
   61 CAACTTAATT TTATTAGGAC AAGGCTGGTG GGCACTGGAG TGGCAACTTC CAGGGCCAGG
  121 AGAGGCACTG GGGAGGGGTC ACAGGGATGC CACCCGGGAT CCTCTAGAGT CGACTTACAG
  181 TTCAATCTTG CATGCAGGGA AGGATTCATC TTGCATAACC ACTGTGCTGC TGCTTGCTAA
  241 AACCATGATG TTGAGATCTT GCTGTTTCTC ATGGGTCTCC TGGTACCATT CCCTCATCAC
  301 TTCTGAGTCA TGAGTTGGGA TCAGTGTCAC CTGCATGTTG CTGCCCCACT GAGCCTTTCC
  361 TTCCAACAAA GCGTCCAGGA CAGCCCGGCT GGGCTCCTCA GCAGCAGGGT CATTCCCACT
  421 CACCACGTAG TAGGAAGACC GCACTCTCTT GAAAAATTCC ACATCAACAT TCTTACTATT
  481 GCTGTGGTTA GGAATGTAGC ACAGGTCCAA ATACACAGGG AGGCCTGGGG GCACAGCAGA
  541 TGACTTGGTC GTCTTGGTAG TTCCTGGTCC TGCAGTGGCG GTCTTGGCCG ACTTGGATGC
  601 AGAGGCATTG GCAGCATTCT TGGTCTCCTT GTCTTTCTCT TCCCCACGTG CAGCTTTGAC
  661 CTCAGGAGTG GTGGTGGGTT TTGCTGCCTT TTCCACAGAT TCTTTCTTCT TAGGAGAAGC
  721 CACCCTGGAC ACTTTATCCG ANGANTCTTT CAAGCCCGCT GGTTTTGGTG AAGCTGCCNA
  781 GGGCTTANAC TTCCCATCAC TCTTTTTGAC ANGTGAAGAT GANTTGGTCT TTGTACCTGG
  841 CTTTTTGGTT TTGGNCTTCT CTTTCANANC TTTCNTTANA GCTTTGCCCA GGTTCTGNTC
  901 NANGGNNCAA NGNCTCTGGG NCCACCATGG ANNNNTCNNN NGGCGNNNNN AANGNNTGCN
  961 NTNNNAANTN NNCGGNNNCN AGCNNGNCGN CNTCGNCNTN NNANNNNNNN GNNNNNNNAT
1021 TNNNANNGNN NNNNNNANCN ANNNCTGNNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, ori
Sequence file: RDB17873_A9L9c.seq check
>D06426A1_A9L9_1_SV40pro_ori_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGCC TCTGAGCNNT TCNNGAAGTA GTGAGGAGGC
   61 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCTCGAGG AACTGAAAAA CCAGAAAGTT
  121 AATTCCCTAT AGTGAGTCGT ATTAAATTCG TAATCATGTC ATAGCTGTTT CCTGTGTGAA
  181 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT
  241 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC
  301 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
  361 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
  421 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
  481 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA
  541 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGNNNNNNNT CACAAAAATC
  601 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
  661 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
  721 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
  781 CGGTGTAGGT CGTTCGCTCC NAGCTGGGCT GTGTGCNCGA NNCCCCCGTT CAGCCCGACC
  841 GCNGCGCCTT ATCCGGTAAC TATCGTCTNG AGTCCAACCC GGTAAGANNC GACTTATCGC
  901 CNCTGNCANC AGCCACNNGT AACNNNANTA NCANANCGAN GNATGNNNNC GGNGCTNCNN
  961 AGNTCNNGAA NTGGTGGCCT NACNNNNNNT NCNCTANNNN NNNGNNNTTN GNNTCTGNNN
1021 NNNNNTNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yonashiro, R., Mitochondrial ubiquitin ligase MITOL blocks S-nitrosylated MAP1B-light chain 1-mediated mitochondrial dysfunction and neuronal cell death. Proc. Natl. Acad. Sci. U.S.A. 109 (7): 2382-2387 (2012). PMID 22308378. [link to RRC of NBRP]