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CSII-EF-IRES2-Bsd-RfA

Lentivirus expression vector with Gateway (R) cloning, EF-1 alpha promoter, IRES-Bsd-RfA.

Catalog number RDB17869
Resource name CSII-EF-IRES2-Bsd-RfA
Alternative name CS-EF-IB-RfA
Clone info. Lentivirus expression vector, backbone vector. Gateway (R) cloning. EF-1 alpha promoter, IRES-Bsd-RfA.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB. Lentivirus transfection: Bsd.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name Aspergillus terreus blasticidin S deaminase (BSD) cDNA
Depositor|Developer Fujii, Masahiro | Higuchi, Masaya |
Other clones in our bank

External Database
Aspergillus terreus blasticidin S deaminase (BSD)

            Reference sequence
              
             
            Sequence (full) RDB17869zzk02.seq provided by the depositor
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Niigata University (ip@adm.niigata-u.ac.jp). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
            Additional terms and conditions:
            The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
            Ordering Please visit Ordering instruction.[link] 
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            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007)。営利機関の利用希望者は、事前に利用条件等につき寄託者(連絡先:新潟大学 ip@adm.niigata-u.ac.jp)と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
            付加的提供条件:
            レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB17869 CSII-EF-IRES2-Bsd-RfA DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17869_A9Lhp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F
            Region: EF-1alpha pro, RfA 5' (attR1, CmR)
            Sequence file: RDB17869_A9Lha.seq check
            >D6420A4_B0Cb_1_EF1a-C_F_A03_03_ABI24.ab1
                1 NNNNNNNNNN NNTTTTNANT TTGGNNCTTG GTTNNTTCTC AAGCCTCAGA CAGTGGTTCA
               61 AAGTTTTTTT CTTCCNTTTC AGGTGTCGTG AACACGCTAC CGGTCTCGAG AATTATCACA
              121 AGTTTGTACA AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT
              181 TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA
              241 TGGCGGCCGC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT
              301 GGATTTTGAG TTAGGATCCG TCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA
              361 AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG
              421 CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT
              481 TTTTAAAGAC CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG
              541 CCCGCCTGAT GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA
              601 TATGGGATAG TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT
              661 CGCTCTGGAG TGAATACCAC GACGATTTCC GGCAGTTTCT ACNCATATAT TCGCAAGATG
              721 TGGCGTGTTA CGGTGAAAAC CTGNCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT
              781 TCGTCTCANC CAATCCCTGG GTGAGTTTCA CCNNNTTTTG ATTTAAACGT GGCCNANNAT
              841 GGACAACTTC TTCGCCNCCG TTTTCNCCAT GGGCANATAT TATACGCAAG GCGANAAGGN
              901 GCTGANGCCG CTNGCGATTC NNGTTCATCA TGCCGTTTGT GANGNNTTCC ATGTCGGCAN
              961 ANNGNTNNAN NGAATTACAN NAGTNNTGCN ANGANTGGNN NNGNCGGGGC NNAAACNCGT
            1021 GNNNNCNGNN TTNNTAAAAN CNNNNNANNN NNNNNNN
            //
            Primer: IRES-R
            Region: IRES2, RfA 3' (attR2, ccdB)
            Sequence file: RDB17869_A9Lhb.seq check
            >D6420A4_B0Cb_1_IRES-R_B06_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGTT AGGGGGGGGG GNNGGANAGG GGCGGATCCG
               61 CGGCCGCTGC AGAATTATCA CCACTTTGTA CAAGAAAGCT GAACGAGAAA CGTAAAATGA
              121 TATAAATATC AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA
              181 AACACAACAT ATCCAGTCAC TATGGTCGAC CTGCAGACTG GCTGTGTATA AGGGAGCCTG
              241 ACATTTATAT TCCCCAGAAC ATCAGGTTAA TGGCGTTTTT GATGTCATTT TCGCGGTGGC
              301 TGAGATCAGC CACTTCTTCC CCGATAACGG AGACCGGCAC ACTGGCCATA TCGGTGGTCA
              361 TCATGCGCCA GCTTTCATCC CCGATATGCA CCACCGGGTA AAGTTCACGG GAGACTTTAT
              421 CTGACAGCAG ACGTGCACTG GCCAGGGGGA TCACCATCCG TCGCCCGGGC GTGTCAATAA
              481 TATCACTCTG TACATCCACA AACAGACGAT AACGGCTCTC TCTTTTATAG GTGTAAACCT
              541 TAAACTGCAT TTCACCAGCC CCTGTTCTCG TCAGCAAAAG AGCCGTTCAT TTCAATAAAC
              601 CGGGCGACCT CAGCCATCCC TTCCTGATTT TCCGCTTTCC AGCGTTCGGC ACGCAGACGA
              661 CGGGCTTCAT TCTGCATGGT TGTGCTTACC AGACCGGAGA TATTGACATC ATATATGCCT
              721 TGAGCAACTG ATAGCTGTCG CTGTCAACTG TCACTGTAAT ACGCTGCTTC ATAGCATACC
              781 TCTTTTTGAC ATACTTCNGG TATACNTATC AGTATATATT CTTATACCGC AAAAATCAGC
              841 GCGCAAATAC GCATACTGTT ATCTGGCTTT TANNAANNCC GGANCCACGC GTTTACNCCC
              901 CGCCCTGCCA CTCNTCGCAN TACTGTTGTA ATTCATNNAN CATTCTGCCN ANNNGGNNNN
              961 NCNTCNCAAA CGGCATGATG AACCTGAATC GNNAGCNGCN TCAGCNNNNT NGTCGCNNNG
            1021 NGNANANNAT TTNNNNNNNN NN
            //
            Primer: PRE_rev
            Region: PRE, BSD, IRES2 3'
            Sequence file: RDB17869_A9Lhc.seq check
            >D6420A4_B0Cb_1_PRE_rev_C06_09_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNCNN NNNNGGANCN NCATAGTTAA GAATACCNGT CAATCTTTCA
               61 CAAATTTTGT AATCCAGAGG TTGATTATCG ATAAGCTTGA TCCCTCGATG TTAACTCTAG
              121 AGGCTCGACT TAGCCCTCCC ACACATAACC AGAGGGCAGC AATTCACGAA TCCCAACTGC
              181 CGTCGGCTGT CCATCACTGT CCTTCACTAT GGCTTTGATC CCAGGATGCA GATCGAGAAG
              241 CACCTGTCGG CACCGTCCGC AGGGGCTCAA GATGCCCCTG TTCTCATTTC CGATCGCGAC
              301 GATACAAGTC AGGTTGCCAG CTGCCGCAGC AGCAGCAGTG CCCAGCACCA CGAGTTCTGC
              361 ACAAGGTCCC CCAGTAAAAT GATATACATT GACACCAGTG AAGATGCGGC CGTCGCTAGA
              421 GAGAGCTGCG CTGGCGACGC TGTAGTCTTC AGAGATGGGG ATGCTGTTGA TTGTAGCCGT
              481 TGCTCTTTCA ATGAGGGTGG ATTCTTCTTG AGACAAAGGC TTGGCCATGG TTGTGGCCAT
              541 ATTATCATCG TGTTTTTCAA AGGAAAACCA CGTCCCCGTG GTTCGGGGGG CCTAGACGTT
              601 TTTTTAACCT CGACTAAACA CATGTAAAGC ATGTGTACCG AGGCCCCAGA TCAGATCCCA
              661 TACAATGGGG TACCTTCTGG GCATCCTTCA GCCCCTTGTT GAATACGCTT GAGGAGAGCC
              721 ATTTGACTCT TTCCACAACT ATCCAACTCA CAACGTGGCA CTGGGGTTGT GCCGCCTTTG
              781 CAGGTGTATC TTATACACGT GGCTTTTGGC CGCAGANGCA CCTGTCGCCA GGTGGGGGGG
              841 TTCCGCTGCC TGCAAAGGGT CNCTACAGAC GTTGTTTGTC TTCAAGAANC TTCCNGANGA
              901 NNTGNTTCCT TCNCGACATT CNACAGANCT TGNATTCCTT TGGCGANAGG GGAAAGACCC
              961 CTANGAATGN TCGNCAANAA NANAGGGNNN NGNTTNNCNG GNNNCNCNCA TTGNCAANNN
            1021 NNNNNNN
            //
            Primer: CMV_Forward
            Region: CMV pro, 5'LTR, HIV-1 psi
            Sequence file: RDB17869_A9Lhd.seq check
            >D6420A4_B0Cb_1_CMV_Forward_D06_12_ABI24.ab1
                1 NNNNNNNNNN NNNNNTGNNG CNNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
               61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
              121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
              181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
              241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
              301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
              361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
              421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
              481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
              541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
              601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
              661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
              721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA
              781 TCTTCAGACC TGGAGGANGA GATATGAGGG ANAATTGGAG AAGTGAATTA TATANATATA
              841 AAGTAGTAAA AATTGNACCN TTAGGANTAG CACCCACCNA NGCAAAGANA AGAGTGGTGC
              901 AGANAGAAAA AAGANCAGTG GGAATAGGAN CTTTGNNCCT NGGGNTCTTG GGANCAGCNN
              961 NAAGCNCTAT GGGCNNANCG NNNNNGACNC NNNNNNNNCN NNNNNGNNNN TNNNNGNCTN
            1021 NNNTNNNNNN NNNN
            //
            Primer: RRE_check
            Region: RRE
            Sequence file: RDB17869_A9Lhe.seq check
            >D6420A4_B0Cb_1_RRE_check_E06_15_ABI24.ab1
                1 NNNNNNNNNN NNNTNNNNNN TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT
               61 GTATTAAGCT TGTGTAATTG TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC
              121 AAATCTGTTC CAGAGATTTA TTACTCCAAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA
              181 TGAGTTTTCC AGAGCAACCC CAAATCCCCA GGAGCTGTTG ATCCTTTAGG TATCTTTCCA
              241 CAGCCAGGAT TCTTGCCTGG AGCTGCTTGA TGCCCCAGAC TGTGAGTTGC AACAGATGCT
              301 GTTGCGCCTC AATAGCCCTC AGCAAATTGT TCTGCTGCTG CACTATACCA GACAATAATT
              361 GTCTGGCCTG TACCGTCAGC GTCATTGACG CTGCGCCCAT AGTGCTTCCT GCTGCTCCCA
              421 AGAACCCAAG GAACAAAGCT CCTATTCCCA CTGCTCTTTT TTCTCTCTGC ACCACTCTTC
              481 TCTTTGCCTT GGTGGGTGCT ACTCCTAATG GTTCAATTTT TACTACTTTA TATTTATATA
              541 ATTCACTTCT CCAATTGTCC CTCATATCTC CTCCTCCAGG TCTGAAGATC AGCGGCCCTT
              601 GCTGTGCGGT GGTCTTACTT TTGTTTTGCT CTTCCTCTAT CTTGTCTAAA GCTTCCTTGG
              661 TGTCTTTTAT CTCTATCCTT TGATGCACAC AATAGAGGGT TGCTACTGTA TTATATAATG
              721 ATCTAAGTTC TTCTGATCCT GTCTGAAGGG ATGNNTGTAG CTGTCCCAGT ATTTGTCTAC
              781 AGCCTTCTGA NGTTTCTAAC AGGNCAGGAN TAACTGCGAA TCGTTCTAGC TCCCTGCTTG
              841 CCCATACTAT ATGTTTTAAT TTATATTTTT TCTTTCCCCC TGGCCTTAAC CNAATTTTTT
              901 CCNNTCGCGA TCNAATTCTC CCCCGCTNAA TACTGACGCT CTCGCACCCN TCTCTCTNNT
              961 TCNANCNNNC GCNAGTCNAA ATTTTNGGNN NANTCACCAN NCGNCNNCCC NNNGCNNNNN
            1021 GNNNNGNNNN NNNCANCNNN NCNNANNNNN NNNNN
            //
            Primer: EF1a-N_R
            Region: EF-1alpha pro, cPPT_CTS, RRE
            Sequence file: RDB17869_A9Lhf.seq check
            >D6420A4_B0Cb_1_EF1a-N_R_F03_18_ABI24.ab1
                1 NNNNNNNNNN NNNNNGNNNN NNNNNGCGAN GTGCGCTCTG CCCNCTGACG GGCACCGGAN
               61 CCTCACGAAG CTTGAATTAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT AAACCCGAAA
              121 ATTTTGAATT TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG TTGCTATTAT
              181 GTCTACTATT CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG
              241 GATGAATACT GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT
              301 CTCCACCTTC TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG
              361 TCTGAAACGA TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA
              421 AAACTATTCT TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA
              481 GCCAATTTGT TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT
              541 CTTGTTCATT CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG
              601 TGTAATTGTT AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA
              661 GAGATTTATT ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG
              721 AGCAACCCCA AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCNCA GCCAGGATTC
              781 TTGCCTGGAG CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA
              841 TANCCCTCAN CAAATTGTTC TGCTGCTGCA CTATACCANA NAATAATTGT CTGGNCTGNA
              901 CCGNCAGCGT CATTGACGCT GCGCCNNTAG TGCTTCCTGC TGCTCCCAAN AACCCNAGGA
              961 NNAAAGCTCC TATTCCNNCT GNTCTTTTTT NTCTCTGCAN CANNCNTCNN TTTNNCTNNN
            1021 NNGGNNNTAN NNNNNNNNN
            //
            Primer: BGH_rev2
            Region: BGH pA, 3'LTR, PRE
            Sequence file: RDB17869_A9Lhg.seq check
            >D6420A4_B0Cb_1_BGH_rev2_G06_21_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGGGGGNNG GGCANCAACA GATGGCTGGC AACTAGAAGG
               61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
              121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
              181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
              241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
              301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
              361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
              421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
              481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT
              541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
              601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
              661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
              721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT
              781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC NAAGGGAGAT CCGACTCGTC TGANGGCGAA
              841 NGCGAAGANG CGGAANANGC CGCANANCNG GCAGCANGCC GCGGGAANGA ANGTCCGCTG
              901 GANTGANGGN CGAANGGNNG TANCANAANG NNGTCCCGCG CANAATCNNN GNGGNANCNC
              961 ANGCNANCAG CCNTGGAAAG GANGTCAGCT TNCCNNANNN NCNNCGGNAN TNNNCANTGN
            1021 CNNNCAGNCN ANCCCNNNNN NNGNNNCNNN NNNNNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40pro_ori, Zeo, SV40 pA
            Sequence file: RDB17869_A9Lhh.seq check
            >D6420A4_B0Cb_1_SV40pro_ori_F_H06_24_ABI24.ab1
                1 NNNNNNNNNN NNNGCCGCCT CTGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
               61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
              121 TCAGCACGTG TTGACAATTA ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG
              181 GTGAGGAACT AAACCATGGC CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC
              241 GTCGCCGGAG CGGTCGAGTT CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG
              301 GACGACTTCG CCGGTGTGGT CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC
              361 CAGGTGGTGC CGGACAACAC CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC
              421 GCCGAGTGGT CGGAGGTCGT GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC
              481 GAGATCGGCG AGCAGCCGTG GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC
              541 GTGCACTTCG TGGCCGAGGA GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC
              601 GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC
              661 CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT
              721 AATGGTTACN AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCNCTG
              781 CATTCTAGTT GTGGTTTGTC CNAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG
              841 ACNTCTANCT AGAGCTTGNC GTAATCATGG TCATAGCTGN TTCCTGNGTG AAANTGTTAT
              901 CCGCTCACAA TTNCNCACAA CATACNANCN NNAANCATAA AGTGTAAAGC CTGGGGNGNC
              961 TNNNGAGTGA NCTNNCTCNC ANTNNNTNGN NNNGNNNTCN CNGCCNNNTT TCCANNNNGN
            1021 NNANNNNNNN NNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223.
            reference Takachi, T., Human T-cell leukemia virus type 1 Tax oncoprotein represses the expression of the BCL11B tumor suppressor in T-cells. Cancer Sci. 106 (4): 461-465 (2015). PMID 25613934.
            reference Higuchi, M., Downregulation of proapoptotic Bim augments IL-2-independent T-cell transformation by human T-cell leukemia virus type-1 Tax. Cancer Med. 3 (6): 1605-1614 (2014). PMID 25175936.

            2022.10.11

            GNP_filter3_RDBDEP_html_221010.pl