RDB17868 - CSII-EF-IRES-GFP-RfA

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CSII-EF-IRES-GFP-RfA (#RDB17868)

Lentivirus expression vector with Gateway (R) cloning, EF-1 alpha promoter, IRES-GFP-RfA.

Alternative name	CS-EF-IG-RfA
Clone info.	Lentivirus expression vector, backbone vector. Gateway (R) cloning. EF-1 alpha promoter, IRES-GFP-RfA.
Vector backbone	CSII-EF-MCS (RDB04378) (plasmid)
Size of vector backbone	9.1 kb
Selectable markers	Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions	LB+Ampicillin, Chloramphenicol, 30oC
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name	Aequorea victoria GFP cDNA
Depositor\|Developer	Fujii, Masahiro \| Higuchi, Masaya \|

Sequence (full)	RDB17868zzk03.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Niigata University (ip@adm.niigata-u.ac.jp). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Niigata University (ip@adm.niigata-u.ac.jp). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007)。営利機関の利用希望者は、事前に利用条件等につき寄託者（連絡先：新潟大学 ip@adm.niigata-u.ac.jp）と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。付加的使用条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB17868	CSII-EF-IRES-GFP-RfA	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-EF-IRES-GFP-RfA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17868).

Reference section:

Higuchi, M., Tsubata, C., Kondo, R., Yoshida, S., Takahashi, M., Oie, M., Tanaka, Y., Mahieux, R., Matsuoka, M., Fujii, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17868_A9Lhp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF1a pro, RfA 5' (attR1, CmR) Sequence file: RDB17868_A9Lha.seq
>D6420A3_B0Cb_1_EF1a-C_F_A05_02_ABI24.ab1 1 NNNNNNNNTN NNNNTTTTGA NTTTGGNNCT TGGTTNNTTC TCNAGCCTCA GACAGTGGTT 61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGCT ACCGGTCTCG AGAATTATCA 121 CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA 181 ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC 241 TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT 301 GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA 361 AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA 421 GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC 481 CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT 541 TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT 601 GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC 661 ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA 721 TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA GGNTTTATTG AGAATATGTT 781 TTTCGTCTCA GCCAATCCCT GNGTGAGTTT CACCAGTTTT GANTTAAACG TGGCCNATAT 841 GGACAACTTC TTCNCCCCCG TTTTCNCCAT GGGCAAATAT TATACGCAAG GNGACAAGGT 901 GCTGANGNCG CTGGNNANTC NNNNCATCAT GNCGTTTGNG ANGGCTTNCN TGTCNGCANA 961 ATGCTTANTG NATTACNNCA GTNNTGNGAT GANTGNNNNG GNNGGGCGNA AACNCNNNNN 1021 NCCNNNTTNN TAAAANNNAN ANANNGTATG CGNATTTGNN NNNNNNNTTN NNNGNNNNNN 1081 NNNN //
Primer: ccdB-M_F (Pr0951) Region: RfA 3' (ccdB, attR2), IRES Sequence file: RDB17868_A9Lhb.seq
>D06420A3_A9Lh_1_ccdb-M_F_A05_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN ANNGGCTGNN CTCNGCCNCC GCGAAANTGA NATCAAAAAC 61 GCCATTAACC TGATGTTCTG GGGAATATAA ATGTCAGGCT CCCTTATACA CAGCCAGTCT 121 GCAGGTCGAC CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT 181 ATGCAAAATC TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT 241 CTTGTACAAA GTGGTGATAA TTCTGCAGCG GCCGCTACGT AAATTCCGCC CCCCCCCCCC 301 CTCTCNNNNN NCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC 361 GTTTGTCTAT ATGTTATTTT CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA 421 ACCTGGCCCT GTCTTCTTGA CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT 481 GCAAGGTCTG TTGAATGTCG TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC 541 AACGTCTGTA NCGACCCTTT GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG 601 CGGCCAAAAG CCACGTGTAT AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCNCGT 661 TGTGAGTTGG ANAGTTGTGG AAAGAGTCAA ATGGCTCTCC TCNAGCGTAT TCAACNAGGG 721 NNTGANNGAN GCCCANANNG TACCCCATTG TATGGGNTCT GATCTGGGGC CTCGGTGNAC 781 ATGCTTTACA TGTGTTTAGT CNAGGTTAAA AAAANGTCTA NGCCCCCCCN ANNCANNGGN 841 NNGTGNTTTT CCTTTGAAAA ANNCNANGAN NANNATNGNN CNCNNNCNAT GGNNNNNCNN 901 NNNNNNNGAN CTGTNNNNCN GGNNNNNNNN NN //
Primer: IRES-F (Pr0036) Region: IRES, EGFP 5' Sequence file: RDB17868_A9Lhc.seq
>D6420A3_B0Cb_1_IRES-F_C05_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNCGNNG TTANNAAACG TCTAGGCCCC CCGAACCACG 61 GGGANGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG 121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC 181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC 241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC 301 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC 361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC 421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC 481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC 541 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG 601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG 661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC 721 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC 781 GTGANCGCCG CCNGGATCAC TCTCGGCATG GACNANCTGT ACAAGTAAGT CAACATCGAG 841 GGATCAAGCT TATCGATAAT CNACCTCTGG ANTACAAAAT TTGTGAAANA NTGACTGGNA 901 TTCTTANNTA TGTTGCTCCT TTTANNCTAT GTGNANACGC TGCTTTAATG NNTTTGTATC 961 ATGCTATNGC TNCCCGTATN NNTTTCNNTT TCTCCNCCTT GNNTAANTCC NNNNNGNTGT 1021 CNCNTNATNA NNANNNGNNG NNCNGNTNNN CNNNNANNNN NNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE, EGFP 3' Sequence file: RDB17868_A9Lhd.seq
>D6420A3_B0Cb_1_PRE_rev_D02_11_ABI24.ab1 1 NNNNNNNNNN NNNNCGNAAA NGGANCNNNN TAGTTAAGAA TACCAGTCAA TCTTTCNCAA 61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTG ACTTACTTGT 121 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT 181 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT 241 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT 301 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT 361 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA 421 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT 481 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG 541 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT 601 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC 661 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTANGTGG 721 CATCGCCCTC GCCCTCGCCG GANACGCTGA ACTTGTGGNC GTTTACGTCG CCGTCCAGCT 781 CGACCAGGAN GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTTGTGG 841 NCATATTATC ATCGTGTTTT TCAAAGGAAA ACCACGTCCC CGNGGTTCGG GGGGNCTANA 901 NGTTTTTTNA ACCTCNANTA AACACNTGTA AAGCATGNGC ACCGAGGNCC CANATCANAT 961 CCCATACANT GGGGTACCTT CTGGGNANNC TNCNNCCCNN NGNTNNNNNN NCTTGANNAN 1021 ANNNNTTNNN NNNNTTCNNN NNCTATNNNN CNNNNNNNNN N //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5' LTR, HIV-1 psi Sequence file: RDB17868_A9Lhe.seq
>D6420A3_B0Cb_1_CMV_Forward_E05_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGGCGGTA GGCGTGTACG GTGGGAGGTC 61 TATATAAGCA GCGCGTTTTG CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG 121 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT 181 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC 241 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA 301 GGGAAACCAG AGGAGCTCTC TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA 361 GGCGAGGGGC GGCGACTGGT GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG 421 AGAGATGGGT GCGAGAGCGT CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA 481 ATTCGGTTAA GGCCAGGGGG AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC 541 AGGGAGCTAG AACGATTCGC AGTTAATCCT GGCCTGTTAG AAACATCAGA AGGCTGTAGA 601 CAAATACTGG GACAGCTACA ACCATCCCTT CAGACAGGAT CAGAAGAACT TAGATCATTA 661 TATAATACAG TAGCAACCCT CTATTGTGTG CATCAAAGGA TAGAGATAAA AGACACCAAG 721 GAAGCTTTAG ACAAGATAGA GGAAGAGCAA AACAAAAGTA AGACCACCGC ACAGCAAGGG 781 CCGCTGATCT TCANACCTGG AGGANGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT 841 AAATATAAAG TAGTAAAAAT TGAACCNTTA GGAGTAGCNC CCACCAAGGC AAAGAGAANA 901 GTGGTGNANA NAGAAAAAAG ANCANTGGGA ATAGGAGCTT TGTNNCTTGG GTTCTTGGGA 961 NCAGCNNGAN NCNCTATNGG CGCNGCGTCN NTGANNCTGA NGGNANNNNN CNANANNNNT 1021 ATTGNCNGGN NNNNNGNANC NNCNNNNNNA TTTGNTNNNN GNNNNNNNNN N //
Primer: RRE_check (Pr0837) Region: RRE Sequence file: RDB17868_A9Lhf.seq
>D6420A3_B0Cb_1_RRE_check_F05_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNTCTTTTC TTGCTGGTTT TGCGATTCTT CNATTAAGGA 61 GTGTATTAAG CTTGTGTAAT TGTTAATTTC TCTGTCCCAC TCCATCCAGG TCGTGTGATT 121 CCAAATCTGT TCCAGAGATT TATTACTCCA ACTAGCATTC CAAGGCACAG CAGTGGTGCA 181 AATGAGTTTT CCAGAGCAAC CCCAAATCCC CAGGAGCTGT TGATCCTTTA GGTATCTTTC 241 CACAGCCAGG ATTCTTGCCT GGAGCTGCTT GATGCCCCAG ACTGTGAGTT GCAACAGATG 301 CTGTTGCGCC TCAATAGCCC TCAGCAAATT GTTCTGCTGC TGCACTATAC CAGACAATAA 361 TTGTCTGGCC TGTACCGTCA GCGTCATTGA CGCTGCGCCC ATAGTGCTTC CTGCTGCTCC 421 CAAGAACCCA AGGAACAAAG CTCCTATTCC CACTGCTCTT TTTTCTCTCT GCACCACTCT 481 TCTCTTTGCC TTGGTGGGTG CTACTCCTAA TGGTTCAATT TTTACTACTT TATATTTATA 541 TAATTCACTT CTCCAATTGT CCCTCATATC TCCTCCTCCA GGTCTGAAGA TCAGCGGCCC 601 TTGCTGTGCG GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT ATCTTGTCTA AAGCTTCCTT 661 GGTGTCTTTT ATCTCTATCC TTTGATGCAC ACAATAGAGG GTTGCTACTG TATTATATAA 721 TGATCTAAGT TCTTCTGATC CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT 781 ACAGCCTTCT GATGTTTCTA ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT 841 TGCCCATACT ATATGTTTNA ATTTATATTT TTTCTTTCCC CCTGNCCTTA NCCNAANTTT 901 TTCCCATCGC GATCTAATTC TCCCCCGCTN NANACTGACG CTCTCNCACC CNNTCTCTCT 961 CCNTCTANCC TCNGCTAGTC NAANTTTTTN GNNGNANTCN CCAGTCNNCN NCCCNNCNNC 1021 NTNNNGNCNN GNNNNNNTCA NNNNNNNNAN NCCNGNGNNN NNANNNNNNC NNNNNGNNTN 1081 NCNNTTNNNN NTNNNNNNNC NNNNNNNGNN NNNNNNNN //
Primer: EF1a-N_R (Pr0024) Region: EF1a pro, cPPT_CTS, RRE Sequence file: RDB17868_A9Lhg.seq
>D6420A3_B0Cb_1_EF1a-N_R_G05_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGGCGNNGT GCGCTCTGCC NNCTGACGGG CACCGGANCC 61 TCACGAAGCT TGAATTAATT CAAAGTGAAT TTCTGCTGTC CCTGTAATAA ACCCGAAAAT 121 TTTGAATTTT TGTAATTTGT TTTTGTAATT CTTTAGTTTG TATGTCTGTT GCTATTATGT 181 CTACTATTCT TTCCCCTGCA CTGTACCCCC CAATCCCCCC TTTTCTTTTA AAATTGTGGA 241 TGAATACTGC CATTTGTAGA TCCGTTCACT AATCGAATGG ATCTGTCTCT GTCTCTCTCT 301 CCACCTTCTT CTTCTATTCC TTCGGGCCTG TCGGGTCCCC TCGGGGTTGG GAGGTGGGTC 361 TGAAACGATA ATGGTGAATA TCCCTGCCTA ACTCTATTCA CTATAGAAAG TACAGCAAAA 421 ACTATTCTTA AACCTACCAA GCCTCCTACT ATCATTATGA ATAATTTTAT ATACCACAGC 481 CAATTTGTTA TGTTAAACCA ATTCCACAAA CTTGCCCATT TATCTAATTC CAATAATTCT 541 TGTTCATTCT TTTCTTGCTG GTTTTGCGAT TCTTCAATTA AGGAGTGTAT TAAGCTTGTG 601 TAATTGTTAA TTTCTCTGTC CCACTCCATC CAGGTCGTGT GATTCCAAAT CTGTTCCAGA 661 GATTTATTAC TCCAACTAGC ATTCCAAGGC ACAGCAGTGG TGCAAATGAG TTTTCCAGAG 721 CAACCCCAAA TCCCCAGGAG CTGTTGATCC TTTANGTATC TTTCCACAGC CAGGANTCTT 781 GCCTGGAGCT GCTTGANGCC NCAGACTGTG ANTTGCAACA GATGCTGTTG CGCCTCAATA 841 GCCCTCANCA AATTGNTCTG CTGCTGCACT ATACCAGANA ATAATTGTCT GGNCTGNANC 901 GTCAGCGTCA TTGANGCTGC NCCCATANNG CTTCCTGCNG NTCCCNAGAA CCCNANGAAN 961 AAANCTNCNA TTCCCACNGN NNTTTTTTCT CTCTGCANNA NNNNNNNNNT TGNNNTNNNN 1021 GNNNNNANNC CTNNNNNNNN NN //
Primer: BGH_rev2 (Pr0606) Region: BGH p(A), 3'LTR Sequence file: RDB17868_A9Lhh.seq
>D6420A3_B0Cb_1_BGH_rev2_H05_23_ABI24.ab1 1 NNNNNNNNNN NNNNNGGGGG NNGGGNAACN ACAGATGGCT GGCAACTAGA AGGCACAGTC 61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG ACTAAAAGGG 121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC 181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA 241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT 301 GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC 361 CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC 421 TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT 481 GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT 541 CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA GTCCCCCCTT 601 TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC 661 CTGANGTGTG ACTGGAANAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC 721 AGCATTGGTA GCTGCTGTAT TGCTACTTGT GANTGCTCCN TGTTTTTCTA GGTCTCGACN 781 GNATCNATGC GGGGANGCGG CCCAAAGGGA NATCCNANTC NTCTGANGNC GANNNNAANA 841 NNCNNNANNN NNNNNANANN NNNNANCNNN NCNNNGGNNN NNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, Zeo, SV40 p(A) Sequence file: RDB17868_A9Lhi.seq
>D06420A3_B0Cb_1_SV40pro_ori_F_F07_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNGC CTCTGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT 61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA 181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG 241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG 301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG 361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT 421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA 481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT 541 GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG 601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 661 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 721 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 781 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 841 CNACCTCTAG CTAGAGCTNG NCGNAATCAT GGNCATAGCT GTTTCCTGTG TGAANTTGTT 901 ATCCGCTCAC NATTCCNCAC NNCATACGAN NCNGGANGCA TAAAGNGNAA ANCCNNNNNN 961 NGNCNNANTG NNNGANCNNN NNCNCNNTNA NTTGNNNTNN CNNNCANNNN CNNNNTTNNN 1021 NNNNNNGNNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223. [link to RRC of NBRP]
reference	Higuchi, M., Downregulation of proapoptotic Bim augments IL-2-independent T-cell transformation by human T-cell leukemia virus type-1 Tax. Cancer Med. 3 (6): 1605-1614 (2014). PMID 25175936. [link to RRC of NBRP]

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Aequorea victoria GFP

Reference sequence