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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-IRES-GFP-RfA

Lentivirus expression vector with Gateway (R) cloning, EF-1 alpha promoter, IRES-GFP-RfA.

Catalog number RDB17868
Resource name CSII-EF-IRES-GFP-RfA
Alternative name CS-EF-IG-RfA
Clone info. Lentivirus expression vector, backbone vector. Gateway (R) cloning. EF-1 alpha promoter, IRES-GFP-RfA.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Fujii, Masahiro | Higuchi, Masaya |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             
            Sequence (full) RDB17868zzk03.seq provided by the depositor
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Niigata University (ip@adm.niigata-u.ac.jp). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
            Additional terms and conditions:
            The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
            Ordering Please visit Ordering instruction.[link] 
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            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007)。営利機関の利用希望者は、事前に利用条件等につき寄託者(連絡先:新潟大学 ip@adm.niigata-u.ac.jp)と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
            付加的提供条件:
            レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB17868 CSII-EF-IRES-GFP-RfA DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17868_A9Lhp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F
            Region: EF1a pro, RfA 5' (attR1, CmR)
            Sequence file: RDB17868_A9Lha.seq check
            >D6420A3_B0Cb_1_EF1a-C_F_A05_02_ABI24.ab1
                1 NNNNNNNNTN NNNNTTTTGA NTTTGGNNCT TGGTTNNTTC TCNAGCCTCA GACAGTGGTT
               61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGCT ACCGGTCTCG AGAATTATCA
              121 CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA
              181 ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC
              241 TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT
              301 GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA
              361 AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA
              421 GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC
              481 CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT
              541 TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT
              601 GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC
              661 ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA
              721 TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA GGNTTTATTG AGAATATGTT
              781 TTTCGTCTCA GCCAATCCCT GNGTGAGTTT CACCAGTTTT GANTTAAACG TGGCCNATAT
              841 GGACAACTTC TTCNCCCCCG TTTTCNCCAT GGGCAAATAT TATACGCAAG GNGACAAGGT
              901 GCTGANGNCG CTGGNNANTC NNNNCATCAT GNCGTTTGNG ANGGCTTNCN TGTCNGCANA
              961 ATGCTTANTG NATTACNNCA GTNNTGNGAT GANTGNNNNG GNNGGGCGNA AACNCNNNNN
             1021 NCCNNNTTNN TAAAANNNAN ANANNGTATG CGNATTTGNN NNNNNNNTTN NNNGNNNNNN
            1081 NNNN
            //
            Primer: ccdB-M_F
            Region: RfA 3' (ccdB, attR2), IRES
            Sequence file: RDB17868_A9Lhb.seq check
            >D06420A3_A9Lh_1_ccdb-M_F_A05_02_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN ANNGGCTGNN CTCNGCCNCC GCGAAANTGA NATCAAAAAC
               61 GCCATTAACC TGATGTTCTG GGGAATATAA ATGTCAGGCT CCCTTATACA CAGCCAGTCT
              121 GCAGGTCGAC CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT
              181 ATGCAAAATC TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT
              241 CTTGTACAAA GTGGTGATAA TTCTGCAGCG GCCGCTACGT AAATTCCGCC CCCCCCCCCC
              301 CTCTCNNNNN NCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC
              361 GTTTGTCTAT ATGTTATTTT CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA
              421 ACCTGGCCCT GTCTTCTTGA CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT
              481 GCAAGGTCTG TTGAATGTCG TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC
              541 AACGTCTGTA NCGACCCTTT GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG
              601 CGGCCAAAAG CCACGTGTAT AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCNCGT
              661 TGTGAGTTGG ANAGTTGTGG AAAGAGTCAA ATGGCTCTCC TCNAGCGTAT TCAACNAGGG
              721 NNTGANNGAN GCCCANANNG TACCCCATTG TATGGGNTCT GATCTGGGGC CTCGGTGNAC
              781 ATGCTTTACA TGTGTTTAGT CNAGGTTAAA AAAANGTCTA NGCCCCCCCN ANNCANNGGN
              841 NNGTGNTTTT CCTTTGAAAA ANNCNANGAN NANNATNGNN CNCNNNCNAT GGNNNNNCNN
            901 NNNNNNNGAN CTGTNNNNCN GGNNNNNNNN NN
            //
            Primer: IRES-F
            Region: IRES, EGFP 5'
            Sequence file: RDB17868_A9Lhc.seq check
            >D6420A3_B0Cb_1_IRES-F_C05_08_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNCGNNG TTANNAAACG TCTAGGCCCC CCGAACCACG
               61 GGGANGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG
              121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
              181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
              241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
              301 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
              361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
              421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
              481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
              541 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG
              601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
              661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC
              721 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC
              781 GTGANCGCCG CCNGGATCAC TCTCGGCATG GACNANCTGT ACAAGTAAGT CAACATCGAG
              841 GGATCAAGCT TATCGATAAT CNACCTCTGG ANTACAAAAT TTGTGAAANA NTGACTGGNA
              901 TTCTTANNTA TGTTGCTCCT TTTANNCTAT GTGNANACGC TGCTTTAATG NNTTTGTATC
              961 ATGCTATNGC TNCCCGTATN NNTTTCNNTT TCTCCNCCTT GNNTAANTCC NNNNNGNTGT
            1021 CNCNTNATNA NNANNNGNNG NNCNGNTNNN CNNNNANNNN NNNNNN
            //
            Primer: PRE_rev
            Region: PRE, EGFP 3'
            Sequence file: RDB17868_A9Lhd.seq check
            >D6420A3_B0Cb_1_PRE_rev_D02_11_ABI24.ab1
                1 NNNNNNNNNN NNNNCGNAAA NGGANCNNNN TAGTTAAGAA TACCAGTCAA TCTTTCNCAA
               61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTG ACTTACTTGT
              121 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT
              181 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT
              241 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT
              301 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT
              361 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA
              421 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT
              481 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG
              541 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT
              601 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC
              661 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTANGTGG
              721 CATCGCCCTC GCCCTCGCCG GANACGCTGA ACTTGTGGNC GTTTACGTCG CCGTCCAGCT
              781 CGACCAGGAN GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTTGTGG
              841 NCATATTATC ATCGTGTTTT TCAAAGGAAA ACCACGTCCC CGNGGTTCGG GGGGNCTANA
              901 NGTTTTTTNA ACCTCNANTA AACACNTGTA AAGCATGNGC ACCGAGGNCC CANATCANAT
              961 CCCATACANT GGGGTACCTT CTGGGNANNC TNCNNCCCNN NGNTNNNNNN NCTTGANNAN
            1021 ANNNNTTNNN NNNNTTCNNN NNCTATNNNN CNNNNNNNNN N
            //
            Primer: CMV_Forward
            Region: CMV pro, 5' LTR, HIV-1 psi
            Sequence file: RDB17868_A9Lhe.seq check
            >D6420A3_B0Cb_1_CMV_Forward_E05_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGGCGGTA GGCGTGTACG GTGGGAGGTC
               61 TATATAAGCA GCGCGTTTTG CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG
              121 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT
              181 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC
              241 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA
              301 GGGAAACCAG AGGAGCTCTC TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA
              361 GGCGAGGGGC GGCGACTGGT GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG
              421 AGAGATGGGT GCGAGAGCGT CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA
              481 ATTCGGTTAA GGCCAGGGGG AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC
              541 AGGGAGCTAG AACGATTCGC AGTTAATCCT GGCCTGTTAG AAACATCAGA AGGCTGTAGA
              601 CAAATACTGG GACAGCTACA ACCATCCCTT CAGACAGGAT CAGAAGAACT TAGATCATTA
              661 TATAATACAG TAGCAACCCT CTATTGTGTG CATCAAAGGA TAGAGATAAA AGACACCAAG
              721 GAAGCTTTAG ACAAGATAGA GGAAGAGCAA AACAAAAGTA AGACCACCGC ACAGCAAGGG
              781 CCGCTGATCT TCANACCTGG AGGANGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT
              841 AAATATAAAG TAGTAAAAAT TGAACCNTTA GGAGTAGCNC CCACCAAGGC AAAGAGAANA
              901 GTGGTGNANA NAGAAAAAAG ANCANTGGGA ATAGGAGCTT TGTNNCTTGG GTTCTTGGGA
              961 NCAGCNNGAN NCNCTATNGG CGCNGCGTCN NTGANNCTGA NGGNANNNNN CNANANNNNT
            1021 ATTGNCNGGN NNNNNGNANC NNCNNNNNNA TTTGNTNNNN GNNNNNNNNN N
            //
            Primer: RRE_check
            Region: RRE
            Sequence file: RDB17868_A9Lhf.seq check
            >D6420A3_B0Cb_1_RRE_check_F05_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNTCTTTTC TTGCTGGTTT TGCGATTCTT CNATTAAGGA
               61 GTGTATTAAG CTTGTGTAAT TGTTAATTTC TCTGTCCCAC TCCATCCAGG TCGTGTGATT
              121 CCAAATCTGT TCCAGAGATT TATTACTCCA ACTAGCATTC CAAGGCACAG CAGTGGTGCA
              181 AATGAGTTTT CCAGAGCAAC CCCAAATCCC CAGGAGCTGT TGATCCTTTA GGTATCTTTC
              241 CACAGCCAGG ATTCTTGCCT GGAGCTGCTT GATGCCCCAG ACTGTGAGTT GCAACAGATG
              301 CTGTTGCGCC TCAATAGCCC TCAGCAAATT GTTCTGCTGC TGCACTATAC CAGACAATAA
              361 TTGTCTGGCC TGTACCGTCA GCGTCATTGA CGCTGCGCCC ATAGTGCTTC CTGCTGCTCC
              421 CAAGAACCCA AGGAACAAAG CTCCTATTCC CACTGCTCTT TTTTCTCTCT GCACCACTCT
              481 TCTCTTTGCC TTGGTGGGTG CTACTCCTAA TGGTTCAATT TTTACTACTT TATATTTATA
              541 TAATTCACTT CTCCAATTGT CCCTCATATC TCCTCCTCCA GGTCTGAAGA TCAGCGGCCC
              601 TTGCTGTGCG GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT ATCTTGTCTA AAGCTTCCTT
              661 GGTGTCTTTT ATCTCTATCC TTTGATGCAC ACAATAGAGG GTTGCTACTG TATTATATAA
              721 TGATCTAAGT TCTTCTGATC CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT
              781 ACAGCCTTCT GATGTTTCTA ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT
              841 TGCCCATACT ATATGTTTNA ATTTATATTT TTTCTTTCCC CCTGNCCTTA NCCNAANTTT
              901 TTCCCATCGC GATCTAATTC TCCCCCGCTN NANACTGACG CTCTCNCACC CNNTCTCTCT
              961 CCNTCTANCC TCNGCTAGTC NAANTTTTTN GNNGNANTCN CCAGTCNNCN NCCCNNCNNC
             1021 NTNNNGNCNN GNNNNNNTCA NNNNNNNNAN NCCNGNGNNN NNANNNNNNC NNNNNGNNTN
            1081 NCNNTTNNNN NTNNNNNNNC NNNNNNNGNN NNNNNNNN
            //
            Primer: EF1a-N_R
            Region: EF1a pro, cPPT_CTS, RRE
            Sequence file: RDB17868_A9Lhg.seq check
            >D6420A3_B0Cb_1_EF1a-N_R_G05_20_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGGCGNNGT GCGCTCTGCC NNCTGACGGG CACCGGANCC
               61 TCACGAAGCT TGAATTAATT CAAAGTGAAT TTCTGCTGTC CCTGTAATAA ACCCGAAAAT
              121 TTTGAATTTT TGTAATTTGT TTTTGTAATT CTTTAGTTTG TATGTCTGTT GCTATTATGT
              181 CTACTATTCT TTCCCCTGCA CTGTACCCCC CAATCCCCCC TTTTCTTTTA AAATTGTGGA
              241 TGAATACTGC CATTTGTAGA TCCGTTCACT AATCGAATGG ATCTGTCTCT GTCTCTCTCT
              301 CCACCTTCTT CTTCTATTCC TTCGGGCCTG TCGGGTCCCC TCGGGGTTGG GAGGTGGGTC
              361 TGAAACGATA ATGGTGAATA TCCCTGCCTA ACTCTATTCA CTATAGAAAG TACAGCAAAA
              421 ACTATTCTTA AACCTACCAA GCCTCCTACT ATCATTATGA ATAATTTTAT ATACCACAGC
              481 CAATTTGTTA TGTTAAACCA ATTCCACAAA CTTGCCCATT TATCTAATTC CAATAATTCT
              541 TGTTCATTCT TTTCTTGCTG GTTTTGCGAT TCTTCAATTA AGGAGTGTAT TAAGCTTGTG
              601 TAATTGTTAA TTTCTCTGTC CCACTCCATC CAGGTCGTGT GATTCCAAAT CTGTTCCAGA
              661 GATTTATTAC TCCAACTAGC ATTCCAAGGC ACAGCAGTGG TGCAAATGAG TTTTCCAGAG
              721 CAACCCCAAA TCCCCAGGAG CTGTTGATCC TTTANGTATC TTTCCACAGC CAGGANTCTT
              781 GCCTGGAGCT GCTTGANGCC NCAGACTGTG ANTTGCAACA GATGCTGTTG CGCCTCAATA
              841 GCCCTCANCA AATTGNTCTG CTGCTGCACT ATACCAGANA ATAATTGTCT GGNCTGNANC
              901 GTCAGCGTCA TTGANGCTGC NCCCATANNG CTTCCTGCNG NTCCCNAGAA CCCNANGAAN
              961 AAANCTNCNA TTCCCACNGN NNTTTTTTCT CTCTGCANNA NNNNNNNNNT TGNNNTNNNN
            1021 GNNNNNANNC CTNNNNNNNN NN
            //
            Primer: BGH_rev2
            Region: BGH p(A), 3'LTR
            Sequence file: RDB17868_A9Lhh.seq check
            >D6420A3_B0Cb_1_BGH_rev2_H05_23_ABI24.ab1
                1 NNNNNNNNNN NNNNNGGGGG NNGGGNAACN ACAGATGGCT GGCAACTAGA AGGCACAGTC
               61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG ACTAAAAGGG
              121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC
              181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA
              241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT
              301 GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC
              361 CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC
              421 TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT
              481 GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT
              541 CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA GTCCCCCCTT
              601 TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC
              661 CTGANGTGTG ACTGGAANAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC
              721 AGCATTGGTA GCTGCTGTAT TGCTACTTGT GANTGCTCCN TGTTTTTCTA GGTCTCGACN
              781 GNATCNATGC GGGGANGCGG CCCAAAGGGA NATCCNANTC NTCTGANGNC GANNNNAANA
            841 NNCNNNANNN NNNNNANANN NNNNANCNNN NCNNNGGNNN NNNNNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40pro_ori, Zeo, SV40 p(A)
            Sequence file: RDB17868_A9Lhi.seq check
            >D06420A3_B0Cb_1_SV40pro_ori_F_F07_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNGC CTCTGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT
               61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
              121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA
              181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG
              241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG
              301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG
              361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT
              421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA
              481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT
              541 GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG
              601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC
              661 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT
              721 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
              781 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT
              841 CNACCTCTAG CTAGAGCTNG NCGNAATCAT GGNCATAGCT GTTTCCTGTG TGAANTTGTT
              901 ATCCGCTCAC NATTCCNCAC NNCATACGAN NCNGGANGCA TAAAGNGNAA ANCCNNNNNN
              961 NGNCNNANTG NNNGANCNNN NNCNCNNTNA NTTGNNNTNN CNNNCANNNN CNNNNTTNNN
            1021 NNNNNNGNNN NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223.
            reference Higuchi, M., Downregulation of proapoptotic Bim augments IL-2-independent T-cell transformation by human T-cell leukemia virus type-1 Tax. Cancer Med. 3 (6): 1605-1614 (2014). PMID 25175936.

            2022.10.12

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