RDB17868 - CSII-EF-IRES-GFP-RfA

Lentivirus expression vector with Gateway (R) cloning, EF-1 alpha promoter, IRES-GFP-RfA.

Distribution information

Catalog number	RDB17868
Resource name	CSII-EF-IRES-GFP-RfA
Alternative name	CS-EF-IG-RfA
Clone info.	Lentivirus expression vector, backbone vector. Gateway (R) cloning. EF-1 alpha promoter, IRES-GFP-RfA. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone	CSII-EF-MCS (plasmid)
Size of vector backbone	9.1 kb
Selectable markers	Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name	Aequorea victoria GFP cDNA
Depositor\|Developer	Fujii, Masahiro \| Higuchi, Masaya \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Niigata University (ip@adm.niigata-u.ac.jp). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution	The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Schedule A (GFP-A) [Link]
Remarks	Remember that you will be working with samples containing infectious virus. Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users)

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007)。営利機関の利用希望者は、事前に利用条件等につき寄託者（連絡先：新潟大学ip@adm.niigata-u.ac.jp）と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
付加的提供条件	レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link] 遺伝子組換え生物の受入れ確認書 [Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Schedule A（GFP-A, 英語版のみ）が追加で必要です。

References and tips

Sequence information

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB17868	CSII-EF-IRES-GFP-RfA	Under QC test. Please contact us.	DNA solution

Electronic file	Nucleotide sequence provided by the depositor RDB17868zzk01.seq

original	Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223.

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

original	Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223.
reference	Higuchi, M., Downregulation of proapoptotic Bim augments IL-2-independent T-cell transformation by human T-cell leukemia virus type-1 Tax. Cancer Med. 3 (6): 1605-1614 (2014). PMID 25175936.