Lentivirus expression vector with Gateway (R) cloning, EF-1 alpha promoter, IRES-GFP-RfA.
Catalog number | RDB17868 |
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Resource name | CSII-EF-IRES-GFP-RfA |
Alternative name | CS-EF-IG-RfA |
Clone info. | Lentivirus expression vector, backbone vector. Gateway (R) cloning. EF-1 alpha promoter, IRES-GFP-RfA. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
Vector backbone | CSII-EF-MCS (plasmid) |
Size of vector backbone | 9.1 kb |
Selectable markers | Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB. |
Growth remarks | Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine. |
Gene/insert name | Aequorea victoria GFP cDNA |
Depositor|Developer | Fujii, Masahiro | Higuchi, Masaya | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Niigata University (ip@adm.niigata-u.ac.jp). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
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Additional terms and conditions for distribution | The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. |
Ordering | Please visit Ordering instruction.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word ![]() Schedule A (GFP-A) [Link] |
Remarks | Remember that you will be working with samples containing infectious virus. Please use Schedule A (GFP-A) additionally. |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB17868 | CSII-EF-IRES-GFP-RfA | Under QC test. Please contact us. | DNA solution |
Electronic file
Electronic file | Nucleotide sequence provided by the depositor RDB17868zzk01.seq |
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Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223. |
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reference | Higuchi, M., Downregulation of proapoptotic Bim augments IL-2-independent T-cell transformation by human T-cell leukemia virus type-1 Tax. Cancer Med. 3 (6): 1605-1614 (2014). PMID 25175936. |
2020.12.24
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