Resource data sheet
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CSII-EF-IRES-GFP-RfA

Lentivirus expression vector with Gateway (R) cloning, EF-1 alpha promoter, IRES-GFP-RfA.

Catalog number RDB17868
Resource name CSII-EF-IRES-GFP-RfA
Alternative name CS-EF-IG-RfA
Clone info. Lentivirus expression vector, backbone vector. Gateway (R) cloning. EF-1 alpha promoter, IRES-GFP-RfA.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name Aequorea victoria GFP cDNA
Originator Fujii, Masahiro | Higuchi, Masaya |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Niigata University (ip@adm.niigata-u.ac.jp). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17868 CSII-EF-IRES-GFP-RfA DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223.

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223.
reference Higuchi, M., Downregulation of proapoptotic Bim augments IL-2-independent T-cell transformation by human T-cell leukemia virus type-1 Tax. Cancer Med. 3 (6): 1605-1614 (2014). PMID 25175936.

2020.10.02

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