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CSII-EF-IRES2-Bsd

Lentivirus expression vector with EF-1 alpha promoter, IRES2-Bsd.

Catalog number RDB17867
Resource name CSII-EF-IRES2-Bsd
Alternative name CSII-EF-IB
Clone info. Lentivirus expression vector with EF-1 alpha promoter, IRES2-Bsd.
Comment Expression was confirmed by the depositor with blasticidin resistance. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r. Lentivirus transfection: Bsd.
Gene/insert name Aspergillus terreus blasticidin S deaminase (BSD) cDNA
Depositor|Developer Fujii, Masahiro | Higuchi, Masaya |
Other clones in our bank

External Database
Aspergillus terreus blasticidin S deaminase (BSD)

            Reference sequence
              
             
            Sequence (full) RDB17867zzk02.seq provided by the depositor
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Niigata University (ip@adm.niigata-u.ac.jp). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
            Additional terms and conditions:
            The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
            Ordering Please visit Ordering instruction.[link] 
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            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007)。営利機関の利用希望者は、事前に利用条件等につき寄託者(連絡先:新潟大学 ip@adm.niigata-u.ac.jp)と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
            付加的提供条件:
            レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB17867 CSII-EF-IRES2-Bsd DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17867_A9Lhp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F
            Region: EF1a pro, IRES2
            Sequence file: RDB17867_A9Lha.seq check
            >D6420A2_B0Cb_3_EF1a-C_F_A01_01_ABI24.ab1
                1 NNNNNNNNNN NTTTTGANTT NGGNNCTTGG TTNNTTCTCN AGCCTCAGAC AGTGGTTCAA
               61 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA ACACGCTACC GGTCTCGAGA ATTCTGCAGC
              121 GGCCGCGGAT CCGCCCCTCT CCCTCCCCCC CCCCTAACGT TACTGGCCGA AGCCGCTTGG
              181 AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG TCTTTTGGCA
              241 ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG GGTCTTTCCC
              301 CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG
              361 CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC CCCCCACCTG
              421 GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA AAGGCGGCAC
              481 AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG CTCTCCTCAA
              541 GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG GGATCTGATC
              601 TGGGGCCTCG GTACACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC
              661 CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC
              721 ATGGCCAAGC CTTTGTCTCA AGAAGAATCC ACCCTCATTG AAAGAGCAAC GGCTACAATC
              781 AACAGCATCC CCATCTCTGA AGACTACAGC GTCGCCAGCG CAGCTCTCTC TAGCGACGGC
              841 CGCATCTTCA CTGGTGTCNA TGTATATCAT TTTACTGGGG GANCTTGTGC ANAACTCGTG
              901 GTGCTGGGCA CTGCTGCTGC TGCGGCANCT GGNAACCTGA CTTGTATCGT CNCGATCNNN
              961 ANTGANAACN GGGGCATCTT GAGCCCCTGN GNANNGNGNC NANNNNNGNT NNTCGATCTG
            1021 NNTNNNNGNN NNNNNNNATN NNGNNNNANA NNNNANGNNN NNNNNNN
            //
            Primer: PRE_rev
            Region: PRE, BSD, IRES2
            Sequence file: RDB17867_A9Lhb.seq check
            >D6420A2_B0Cb_3_PRE_rev_B01_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNGNNNN GGANCNNNNT AGTTAAGAAT ACCNGTCAAT CTTTCNCAAA
               61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGAGGC
              121 TCGACTTAGC CCTCCCACAC ATAACCAGAG GGCAGCAATT CACGAATCCC AACTGCCGTC
              181 GGCTGTCCAT CACTGTCCTT CACTATGGCT TTGATCCCAG GATGCAGATC GAGAAGCACC
              241 TGTCGGCACC GTCCGCAGGG GCTCAAGATG CCCCTGTTCT CATTTCCGAT CGCGACGATA
              301 CAAGTCAGGT TGCCAGCTGC CGCAGCAGCA GCAGTGCCCA GCACCACGAG TTCTGCACAA
              361 GGTCCCCCAG TAAAATGATA TACATTGACA CCAGTGAAGA TGCGGCCGTC GCTAGAGAGA
              421 GCTGCGCTGG CGACGCTGTA GTCTTCAGAG ATGGGGATGC TGTTGATTGT AGCCGTTGCT
              481 CTTTCAATGA GGGTGGATTC TTCTTGAGAC AAAGGCTTGG CCATGGTTGT GGCCATATTA
              541 TCATCGTGTT TTTCAAAGGA AAACCACGTC CCCGTGGTTC GGGGGGCCTA GACGTTTTTT
              601 TAACCTCGAC TAAACACATG TAAAGCATGT GTACCGAGGC CCCAGATCAG ATCCCATACA
              661 ATGGGGTACC TTCTGGGCAT CCTTCAGCCC CTTGTTGAAT ACGCTTGAGG AGAGCCATTT
              721 GACTCTTTCC ACAACTATCC AACTCACAAC GTGGCACTGG GGTTGTGCCG CCTTTGCAGG
              781 TGTATCTTAT ACACGTGGCT TTTGGCCGCA NANGCACCTG TCGCCAGGNG GGGGGTTCCG
              841 CTGCCTGCAA AGGGTCGCTA CAGANGTTGT TTGTCTTCNA GANNCTTCCA GAGGANNTGC
              901 TTNCTTCACG ANATTCNACA NANCTTGCAT TCCTTTGNNG ANANGGGAAA GANCCNTNNN
              961 AATGNTCGTC NNNANNANNN GNNNNGGNTT NCNNGNNNNN CNCNTTGCCN AAANNNNNNA
            1021 NNNNNNTGNA ANANANNTNN NNNNNANNNN NNNNNN
            //
            Primer: CMV_Forward
            Region: CMV pro, 5'LTR, HIV-1 psi
            Sequence file: RDB17867_A9Lhc.seq check
            >D6420A2_B0Cb_3_CMV_Forward_C01_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNTG NNNNNNNGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
               61 AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC
              121 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC
              181 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT
              241 AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA
              301 ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA
              361 GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA
              421 TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG
              481 GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA
              541 GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT
              601 ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA
              661 TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC
              721 TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGGGCCGCT
              781 GATCTTCAGA CCTGGAGGAN GAGATATGAN GGACAATTGG AGAAGTGAAT TATATAAATA
              841 TNAAGTAGTA AAAATTGAAC CATTANGAGT AGCACCCACC NNGGCAAAGA GAANANTGNT
              901 GCAGANAGAA AAAAGANCAG TGGGAATANG ANCTTTGNTC CTNNGNNNCT TGGGANCAGC
              961 NNNAGCACTA TGGGCGCAGC GNNNNTGNNN CTGNNGGTAC NNNNNANNNN NTTANTGNCT
            1021 NNNNTNNNGN AGCANCNNNN NNNTNNNNNN NGNNNNNNGN NNNNNNNNN
            //
            Primer: RRE_check
            Region: RRE
            Sequence file: RDB17867_A9Lhd.seq check
            >D6420A2_B0Cb_3_RRE_check_F04_16_ABI24.ab1
                1 NNNNNNNNNN NNTNGNTNNT TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAAGGAGTG
               61 TATTAAGCTT GTGTAATTGT TAATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGATTCCA
              121 AATCTGTTCC AGAGATTTAT TACTCCAACT AGCATTCCAA GGCACAGCAG TGGTGCAAAT
              181 GAGTTTTCCA GAGCAACCCC AAATCCCCAG GAGCTGTTGA TCCTTTAGGT ATCTTTCCAC
              241 AGCCAGGATT CTTGCCTGGA GCTGCTTGAT GCCCCAGACT GTGAGTTGCA ACAGATGCTG
              301 TTGCGCCTCA ATAGCCCTCA GCAAATTGTT CTGCTGCTGC ACTATACCAG ACAATAATTG
              361 TCTGGCCTGT ACCGTCAGCG TCATTGACGC TGCGCCCATA GTGCTTCCTG CTGCTCCCAA
              421 GAACCCAAGG AACAAAGCTC CTATTCCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT
              481 CTTTGCCTTG GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA
              541 TTCACTTCTC CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCCTTG
              601 CTGTGCGGTG GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT
              661 GTCTTTTATC TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA
              721 TCTAAGTTCT TCTGATCCTG TCTGAAGGGA NGNTTGTAGC TGTCCCAGTA TTTGTCTACA
              781 GCCTTCTGAT GTTTCTAACA GGCCAGGANT AACTGCGAAT CGTTCTANCT CCCTGCTTGC
              841 CCATACTATA TGTTTTAATT TATATTTTTT CTTTCCCCCT GNCCTTAACC GAATTTTTTC
              901 CCNTCGCGAT CTAATTCTCC CCCGCTTAAT ACTGANGCTC TCGCACCNNT CTCTCTCCNT
              961 CTANCCNNCN CNAGNCAAAA TTTTNGGNGN ANNCNCCANN CGNNNNCCNN CNNNNNNNGN
            1021 NNNGNNNNNN NCANNNNNNN NNN
            //
            Primer: EF1a-N_R
            Region: cPPT_CTS
            Sequence file: RDB17867_A9Lhe.seq check
            >D6420A2_B0Cb_3_EF1a-N_R_E01_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNGTGCGC TCTGCCCNCT GACGGGCACC GGANCCTCAC
               61 GAAGCTTGAA TTAATTCAAA GTGAATTTCT GCTGTCCCTG TAATAAACCC GAAAATTTTG
              121 AATTTTTGTA ATTTGTTTTT GTAATTCTTT AGTTTGTATG TCTGTTGCTA TTATGTCTAC
              181 TATTCTTTCC CCTGCACTGT ACCCCCCAAT CCCCCCTTTT CTTTTAAAAT TGTGGATGAA
              241 TACTGCCATT TGTAGATCCG TTCACTAATC GAATGGATCT GTCTCTGTCT CTCTCTCCAC
              301 CTTCTTCTTC TATTCCTTCG GGCCTGTCGG GTCCCCTCGG GGTTGGGAGG TGGGTCTGAA
              361 ACGATAATGG TGAATATCCC TGCCTAACTC TATTCACTAT AGAAAGTACA GCAAAAACTA
              421 TTCTTAAACC TACCAAGCCT CCTACTATCA TTATGAATAA TTTTATATAC CACAGCCAAT
              481 TTGTTATGTT AAACCAATTC CACAAACTTG CCCATTTATC TAATTCCAAT AATTCTTGTT
              541 CATTCTTTTC TTGCTGGTTT TGCGATTCTT CAATTAAGGA GTGTATTAAG CTTGTGTAAT
              601 TGTTAATTTC TCTGTCCCAC TCCATCCAGG TCGTGTGATT CCAAATCTGT TCCAGAGATT
              661 TATTACTCCA ACTAGCATTC CNAGGCACAG CAGTGGTGCA AATGAGTTTT CCAGAGCAAC
              721 CCCAAATCCC CAGGAGCTGT TGATCCTTTA GGTATCTTTC CACAGCCAGG ANTCTTGCCT
              781 GGAGCTGCTT GATGCCCCAG ACTGTGANTT GCAACAGATG CTGTTGCNCC TCAATANCCC
              841 TCANCAAATT GTTCTGCTGC TGCACTATAC CANANAATAN NTGNCTGGNC TGTANCGTCA
              901 GCGTCATTGA NGCTGCGCCC ATANTGCTTC CTGNTGNTCC NNANANNCNN NGNAANAANN
              961 TCCTATTCCC NCTGCTCTTT TTTCTCTCTG NANNNCTNNN CNCNTNNNNT NNGNNGGGNG
            1021 CNNNNCCNNN NGNNNCNATT TTTANNNNTT NNNATTNNNN NNNNNN
            //
            Primer: BGH_rev2
            Region: BGH p(A), 3'LTR, PRE
            Sequence file: RDB17867_A9Lhf.seq check
            >D6420A2_B0Cb_3_BGH_rev2_F01_16_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NGGGGGNNGG GCANCNACAG ATGGCTGGCA ACTAGAAGGC
               61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA
              121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG
              181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG
              241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG
              301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG
              361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC
              421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG
              481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG
              541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC
              601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA
              661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC
              721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC
              781 TCGACGGTAT CGATGCGGGG ANGCGGCCCA NAGGGAGATC CGACTCGTCT GANGGCGAAN
              841 GCGAAGANGC GGAAGANGCC GCANANCCGG CAGCANGCCG CGGGAANGAA GGTCCNCTGG
              901 ANTGANGGNC GAAGGGANGT ANCAGAANGA CGTCCCGCGC ANAANCCANG NGGNAACACA
              961 NGCGANCAGC CATNGNAAAN NNNGTCANCT TCCCCNNNNN CNNCACNGNA NTNGNCAGTG
            1021 NCCNNNANNC NNANNCCCNG NNNNNCNNNN NNNNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40pro_ori, Zeo, SV40 p(A)
            Sequence file: RDB17867_A9Lhg.seq check
            >D6420A2_B0Cb_3_SV40pro_ori_F_G01_19_ABI24.ab1
                1 NNNNNNNNNN NNNGNNNCCT CTGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
               61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
              121 TCAGCACGTG TTGACAATTA ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG
              181 GTGAGGAACT AAACCATGGC CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC
              241 GTCGCCGGAG CGGTCGAGTT CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG
              301 GACGACTTCG CCGGTGTGGT CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC
              361 CAGGTGGTGC CGGACAACAC CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC
              421 GCCGAGTGGT CGGAGGTCGT GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC
              481 GAGATCGGCG AGCAGCCGTG GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC
              541 GTGCACTTCG TGGCCGAGGA GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC
              601 GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC
              661 CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT
              721 AATGNNTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG
              781 CATTCTAGTT GTGGTTTGTC CNAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG
              841 ACCTCTANCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAANTGTTNT
              901 CCGCTCACNA TTCCNCNCNA CATACGANNC GGAANCATAA AGTGNAAAGC CTGGGGNGNC
              961 TAATGAGTGA NCTNACTCAC NTNNANTNNN NTTGCGCTCA CTGNCCNNNT NNCAGNNNGG
             1021 NAANCNNNNC NNGNNNNNNG NANNNNNGNN NCNGNCNNNN NNNNNGGNNN NNNNNTNNNN
            1081 NNNNGGNNNN NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223.

            2022.10.11

            GNP_filter3_RDBDEP_html_221010.pl