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CSII-EF-IRES2-Bsd (#RDB17867)

Lentivirus expression vector with EF-1 alpha promoter, IRES2-Bsd.

Alternative name CSII-EF-IB
Clone info. Lentivirus expression vector with EF-1 alpha promoter, IRES2-Bsd.
Comment Expression was confirmed by the depositor with blasticidin resistance.
Vector backbone CSII-EF-MCS (RDB04378) (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Ampicillin (E. coli), Bsd (lentivirus transfection). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions LB+Ampicillin, 37oC
Gene/insert name Aspergillus terreus blasticidin S deaminase (BSD) cDNA
Depositor|Developer Fujii, Masahiro | Higuchi, Masaya |
 
Sequence (full) RDB17867hts01.seq checkAssembled from experimentally sequenced data.
Publication Higuchi, M., J. Virol. 81 (21): 11900-11907 (2007). [link to RRC of NBRP]
Test sheet RDB17867_B3Jrp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Niigata University (ip@adm.niigata-u.ac.jp). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007)。営利機関の利用希望者は、事前に利用条件等につき寄託者(連絡先:新潟大学 ip@adm.niigata-u.ac.jp)と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
MTAに書く付加的使用条件:
レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17867 CSII-EF-IRES2-Bsd DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSII-EF-IRES2-Bsd was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17867).

Reference section:

Higuchi, M., Tsubata, C., Kondo, R., Yoshida, S., Takahashi, M., Oie, M., Tanaka, Y., Mahieux, R., Matsuoka, M., Fujii, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223. [link to RRC of NBRP]