RDB17867 - CSII-EF-IRES2-Bsd

Sequence (full)	RDB17867zzk02.seq provided by the depositor

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Niigata University (ip@adm.niigata-u.ac.jp). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007)。営利機関の利用希望者は、事前に利用条件等につき寄託者（連絡先：新潟大学 ip@adm.niigata-u.ac.jp）と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。付加的提供条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB17867	CSII-EF-IRES2-Bsd	DNA solution

Featured content	Lentivirus Vector (English text)
Featured content	Lentivirus Vector (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
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Primer: EF1a-C_F (Pr0168) Region: EF1a pro, IRES2 Sequence file: RDB17867_A9Lha.seq
>D6420A2_B0Cb_3_EF1a-C_F_A01_01_ABI24.ab1 1 NNNNNNNNNN NTTTTGANTT NGGNNCTTGG TTNNTTCTCN AGCCTCAGAC AGTGGTTCAA 61 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA ACACGCTACC GGTCTCGAGA ATTCTGCAGC 121 GGCCGCGGAT CCGCCCCTCT CCCTCCCCCC CCCCTAACGT TACTGGCCGA AGCCGCTTGG 181 AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG TCTTTTGGCA 241 ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG GGTCTTTCCC 301 CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG 361 CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC CCCCCACCTG 421 GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA AAGGCGGCAC 481 AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG CTCTCCTCAA 541 GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG GGATCTGATC 601 TGGGGCCTCG GTACACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC 661 CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC 721 ATGGCCAAGC CTTTGTCTCA AGAAGAATCC ACCCTCATTG AAAGAGCAAC GGCTACAATC 781 AACAGCATCC CCATCTCTGA AGACTACAGC GTCGCCAGCG CAGCTCTCTC TAGCGACGGC 841 CGCATCTTCA CTGGTGTCNA TGTATATCAT TTTACTGGGG GANCTTGTGC ANAACTCGTG 901 GTGCTGGGCA CTGCTGCTGC TGCGGCANCT GGNAACCTGA CTTGTATCGT CNCGATCNNN 961 ANTGANAACN GGGGCATCTT GAGCCCCTGN GNANNGNGNC NANNNNNGNT NNTCGATCTG 1021 NNTNNNNGNN NNNNNNNATN NNGNNNNANA NNNNANGNNN NNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE, BSD, IRES2 Sequence file: RDB17867_A9Lhb.seq
>D6420A2_B0Cb_3_PRE_rev_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNGNNNN GGANCNNNNT AGTTAAGAAT ACCNGTCAAT CTTTCNCAAA 61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGAGGC 121 TCGACTTAGC CCTCCCACAC ATAACCAGAG GGCAGCAATT CACGAATCCC AACTGCCGTC 181 GGCTGTCCAT CACTGTCCTT CACTATGGCT TTGATCCCAG GATGCAGATC GAGAAGCACC 241 TGTCGGCACC GTCCGCAGGG GCTCAAGATG CCCCTGTTCT CATTTCCGAT CGCGACGATA 301 CAAGTCAGGT TGCCAGCTGC CGCAGCAGCA GCAGTGCCCA GCACCACGAG TTCTGCACAA 361 GGTCCCCCAG TAAAATGATA TACATTGACA CCAGTGAAGA TGCGGCCGTC GCTAGAGAGA 421 GCTGCGCTGG CGACGCTGTA GTCTTCAGAG ATGGGGATGC TGTTGATTGT AGCCGTTGCT 481 CTTTCAATGA GGGTGGATTC TTCTTGAGAC AAAGGCTTGG CCATGGTTGT GGCCATATTA 541 TCATCGTGTT TTTCAAAGGA AAACCACGTC CCCGTGGTTC GGGGGGCCTA GACGTTTTTT 601 TAACCTCGAC TAAACACATG TAAAGCATGT GTACCGAGGC CCCAGATCAG ATCCCATACA 661 ATGGGGTACC TTCTGGGCAT CCTTCAGCCC CTTGTTGAAT ACGCTTGAGG AGAGCCATTT 721 GACTCTTTCC ACAACTATCC AACTCACAAC GTGGCACTGG GGTTGTGCCG CCTTTGCAGG 781 TGTATCTTAT ACACGTGGCT TTTGGCCGCA NANGCACCTG TCGCCAGGNG GGGGGTTCCG 841 CTGCCTGCAA AGGGTCGCTA CAGANGTTGT TTGTCTTCNA GANNCTTCCA GAGGANNTGC 901 TTNCTTCACG ANATTCNACA NANCTTGCAT TCCTTTGNNG ANANGGGAAA GANCCNTNNN 961 AATGNTCGTC NNNANNANNN GNNNNGGNTT NCNNGNNNNN CNCNTTGCCN AAANNNNNNA 1021 NNNNNNTGNA ANANANNTNN NNNNNANNNN NNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5'LTR, HIV-1 psi Sequence file: RDB17867_A9Lhc.seq
>D6420A2_B0Cb_3_CMV_Forward_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNTG NNNNNNNGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT 61 AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC 121 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC 181 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT 241 AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA 301 ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA 361 GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA 421 TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG 481 GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA 541 GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT 601 ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA 661 TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC 721 TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGGGCCGCT 781 GATCTTCAGA CCTGGAGGAN GAGATATGAN GGACAATTGG AGAAGTGAAT TATATAAATA 841 TNAAGTAGTA AAAATTGAAC CATTANGAGT AGCACCCACC NNGGCAAAGA GAANANTGNT 901 GCAGANAGAA AAAAGANCAG TGGGAATANG ANCTTTGNTC CTNNGNNNCT TGGGANCAGC 961 NNNAGCACTA TGGGCGCAGC GNNNNTGNNN CTGNNGGTAC NNNNNANNNN NTTANTGNCT 1021 NNNNTNNNGN AGCANCNNNN NNNTNNNNNN NGNNNNNNGN NNNNNNNNN //
Primer: RRE_check (Pr0837) Region: RRE Sequence file: RDB17867_A9Lhd.seq
>D6420A2_B0Cb_3_RRE_check_F04_16_ABI24.ab1 1 NNNNNNNNNN NNTNGNTNNT TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAAGGAGTG 61 TATTAAGCTT GTGTAATTGT TAATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGATTCCA 121 AATCTGTTCC AGAGATTTAT TACTCCAACT AGCATTCCAA GGCACAGCAG TGGTGCAAAT 181 GAGTTTTCCA GAGCAACCCC AAATCCCCAG GAGCTGTTGA TCCTTTAGGT ATCTTTCCAC 241 AGCCAGGATT CTTGCCTGGA GCTGCTTGAT GCCCCAGACT GTGAGTTGCA ACAGATGCTG 301 TTGCGCCTCA ATAGCCCTCA GCAAATTGTT CTGCTGCTGC ACTATACCAG ACAATAATTG 361 TCTGGCCTGT ACCGTCAGCG TCATTGACGC TGCGCCCATA GTGCTTCCTG CTGCTCCCAA 421 GAACCCAAGG AACAAAGCTC CTATTCCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT 481 CTTTGCCTTG GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA 541 TTCACTTCTC CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCCTTG 601 CTGTGCGGTG GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT 661 GTCTTTTATC TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA 721 TCTAAGTTCT TCTGATCCTG TCTGAAGGGA NGNTTGTAGC TGTCCCAGTA TTTGTCTACA 781 GCCTTCTGAT GTTTCTAACA GGCCAGGANT AACTGCGAAT CGTTCTANCT CCCTGCTTGC 841 CCATACTATA TGTTTTAATT TATATTTTTT CTTTCCCCCT GNCCTTAACC GAATTTTTTC 901 CCNTCGCGAT CTAATTCTCC CCCGCTTAAT ACTGANGCTC TCGCACCNNT CTCTCTCCNT 961 CTANCCNNCN CNAGNCAAAA TTTTNGGNGN ANNCNCCANN CGNNNNCCNN CNNNNNNNGN 1021 NNNGNNNNNN NCANNNNNNN NNN //
Primer: EF1a-N_R (Pr0024) Region: cPPT_CTS Sequence file: RDB17867_A9Lhe.seq
>D6420A2_B0Cb_3_EF1a-N_R_E01_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGTGCGC TCTGCCCNCT GACGGGCACC GGANCCTCAC 61 GAAGCTTGAA TTAATTCAAA GTGAATTTCT GCTGTCCCTG TAATAAACCC GAAAATTTTG 121 AATTTTTGTA ATTTGTTTTT GTAATTCTTT AGTTTGTATG TCTGTTGCTA TTATGTCTAC 181 TATTCTTTCC CCTGCACTGT ACCCCCCAAT CCCCCCTTTT CTTTTAAAAT TGTGGATGAA 241 TACTGCCATT TGTAGATCCG TTCACTAATC GAATGGATCT GTCTCTGTCT CTCTCTCCAC 301 CTTCTTCTTC TATTCCTTCG GGCCTGTCGG GTCCCCTCGG GGTTGGGAGG TGGGTCTGAA 361 ACGATAATGG TGAATATCCC TGCCTAACTC TATTCACTAT AGAAAGTACA GCAAAAACTA 421 TTCTTAAACC TACCAAGCCT CCTACTATCA TTATGAATAA TTTTATATAC CACAGCCAAT 481 TTGTTATGTT AAACCAATTC CACAAACTTG CCCATTTATC TAATTCCAAT AATTCTTGTT 541 CATTCTTTTC TTGCTGGTTT TGCGATTCTT CAATTAAGGA GTGTATTAAG CTTGTGTAAT 601 TGTTAATTTC TCTGTCCCAC TCCATCCAGG TCGTGTGATT CCAAATCTGT TCCAGAGATT 661 TATTACTCCA ACTAGCATTC CNAGGCACAG CAGTGGTGCA AATGAGTTTT CCAGAGCAAC 721 CCCAAATCCC CAGGAGCTGT TGATCCTTTA GGTATCTTTC CACAGCCAGG ANTCTTGCCT 781 GGAGCTGCTT GATGCCCCAG ACTGTGANTT GCAACAGATG CTGTTGCNCC TCAATANCCC 841 TCANCAAATT GTTCTGCTGC TGCACTATAC CANANAATAN NTGNCTGGNC TGTANCGTCA 901 GCGTCATTGA NGCTGCGCCC ATANTGCTTC CTGNTGNTCC NNANANNCNN NGNAANAANN 961 TCCTATTCCC NCTGCTCTTT TTTCTCTCTG NANNNCTNNN CNCNTNNNNT NNGNNGGGNG 1021 CNNNNCCNNN NGNNNCNATT TTTANNNNTT NNNATTNNNN NNNNNN //
Primer: BGH_rev2 (Pr0606) Region: BGH p(A), 3'LTR, PRE Sequence file: RDB17867_A9Lhf.seq
>D6420A2_B0Cb_3_BGH_rev2_F01_16_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NGGGGGNNGG GCANCNACAG ATGGCTGGCA ACTAGAAGGC 61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA 121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG 181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG 241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG 301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG 361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC 421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG 481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG 541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC 601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA 661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC 721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC 781 TCGACGGTAT CGATGCGGGG ANGCGGCCCA NAGGGAGATC CGACTCGTCT GANGGCGAAN 841 GCGAAGANGC GGAAGANGCC GCANANCCGG CAGCANGCCG CGGGAANGAA GGTCCNCTGG 901 ANTGANGGNC GAAGGGANGT ANCAGAANGA CGTCCCGCGC ANAANCCANG NGGNAACACA 961 NGCGANCAGC CATNGNAAAN NNNGTCANCT TCCCCNNNNN CNNCACNGNA NTNGNCAGTG 1021 NCCNNNANNC NNANNCCCNG NNNNNCNNNN NNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, Zeo, SV40 p(A) Sequence file: RDB17867_A9Lhg.seq
>D6420A2_B0Cb_3_SV40pro_ori_F_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNGNNNCCT CTGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT 61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA 121 TCAGCACGTG TTGACAATTA ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG 181 GTGAGGAACT AAACCATGGC CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC 241 GTCGCCGGAG CGGTCGAGTT CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG 301 GACGACTTCG CCGGTGTGGT CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC 361 CAGGTGGTGC CGGACAACAC CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC 421 GCCGAGTGGT CGGAGGTCGT GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC 481 GAGATCGGCG AGCAGCCGTG GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC 541 GTGCACTTCG TGGCCGAGGA GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC 601 GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC 661 CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT 721 AATGNNTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG 781 CATTCTAGTT GTGGTTTGTC CNAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG 841 ACCTCTANCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAANTGTTNT 901 CCGCTCACNA TTCCNCNCNA CATACGANNC GGAANCATAA AGTGNAAAGC CTGGGGNGNC 961 TAATGAGTGA NCTNACTCAC NTNNANTNNN NTTGCGCTCA CTGNCCNNNT NNCAGNNNGG 1021 NAANCNNNNC NNGNNNNNNG NANNNNNGNN NCNGNCNNNN NNNNNGGNNN NNNNNTNNNN 1081 NNNNGGNNNN NNNNNN //

CSII-EF-IRES2-Bsd

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