Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-IRES-GFP

Lentivirus expression vector with EF-1 alpha promoter, IRES-GFP.

Sequence (full)	RDB17866zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Niigata University (ip@adm.niigata-u.ac.jp). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007)。営利機関の利用希望者は、事前に利用条件等につき寄託者（連絡先：新潟大学 ip@adm.niigata-u.ac.jp）と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 付加的提供条件： レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17866_A9Lhp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF1a pro, IRES Sequence file: RDB17866_A9Lha.seq
>D06420A1_B0Cb_3_EF1a-C_F_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNTTTTT NANTTTGGNN CTTGGTTNNT TCTCAAGCCT CAGACAGTGG    61 TTCAAAGTTT TTTTCTTCCN TTTCAGGTGT CGTGAACACG CTACCGGTCT CGAGAATTCT   121 GCAGCGGCCG CTACGTAAAT TCCGCCCCCC CCCCCCCTCT CCCTCCCCCC CCCCTAACGT   181 TACTGGCCGA AGCCGCTTGG AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC   241 CATATTGCCG TCTTTTGGCA ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG   301 CATTCCTAGG GGTCTTTCCC CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA   361 GGAAGCAGTT CCTCTGGAAG CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG   421 GCAGCGGAAC CCCCCACCTG GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA   481 TACACCTGCA AAGGCGGCAC AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG   541 AGTCAAATGG CTCTCCTCAA GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC   601 CCATTGTATG GGATCTGATC TGGGGCCTCG GTGCACATGC TTTACATGTG TTTAGTCGAG   661 GTTAAAAAAA CGTCTAGGCC CCCCNAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACN   721 NNNNNNAANN TGGCCACAAC CATGNNNAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG   781 CCCATCCTGN TCNAGCTGGA CGGNGACNTN AACGGCCACA AGTTCAGCGT GNNNNGNNAG   841 GGCNANGGCN ATGCCNNCTA CGGNAAGNTG NNCNTGAANN TCATCTGCNN CACCGGNNAG   901 CTNNCCGTGN CCTGGCCNNC CNTCNNGANC ANCCTGANCT ANNGCGNGNA NTNNTTNNNN   961 CCGNNNCCCC NANNNCATGA NNCANNNCNN NTTCNTCNAG TNNNNCNTGC CNNNNGCTNN  1021 GTNCNNNANC GCANCNNNNN NNTCNNNNNN NNNGNANNNN NANACNNNNN NNNANNTGNA 1081 NNNNNNNNGG NNNNNNNNNN //
Primer: IRES-F (Pr0036) Region: IRES, EGFP 5' Sequence file: RDB17866_A9Lhb.seq
>D06420A1_B0Cb_3_IRES-F_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTNA GTCNANGNNN NNNNNACGTC TAGGCCCCCC GAACCNCGGG    61 GNNGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG TGAGCAAGGG   121 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG   181 CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT   241 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT   301 GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT   361 CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG   421 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA   481 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA   541 CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA   601 CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA   661 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGANCACCCA   721 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT   781 GACCGCCGCC GGGATCACTC TCGGCATGGA CGANCTGTAC AAGTAAGTCA ACATCGAGGG   841 ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT GTGNANGANT GACTGGNATT   901 CTTANCTATG TTGCTCCTTT TACNCTATGN GNANACNCTG CTTNNATGNC TTTGNATCAT   961 GNTATTGNTT CCCGTATGGN TTTCATTTTC TCNNNCNNNG NATAAATCCN NNNNGNTGNC 1021 NCNTTNNNNN NNANNNNNNN NNN //
Primer: PRE_rev (Pr0250) Region: PRE, EGFP 3' Sequence file: RDB17866_A9Lhc.seq
>D06420A1_B0Cb_3_PRE_rev_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNN NGGAGCNNCN TAGTTAAGAA TACCNGTCAA TCTTTCACAA    61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTG ACTTACTTGT   121 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT   181 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT   241 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT   301 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT   361 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA   421 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT   481 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG   541 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT   601 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC   661 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTAGGTGG   721 CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GNTTACGTCG CCGTCCAGCT   781 CGACCAGNAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGNTGNGG   841 CCATATTATC ATCGTGTTTT TCAAAGGAAA ACCACGTCCC CGNGGTTCNG GGGGGCCTAN   901 NCNTTTTTTT NANCNTCGAC TAAACNCNTG TNANNCATGT GCNCCNAGGN CCCCNNATCA   961 NATCCNNTAN NATGGGGNNN NCTTCTGGGN NNCCTNNNNN CCNNNGNTNN AANNCNCNNG 1021 NNGNNNNNNC ATTNNNNNNN TTNNCNNCNN NTANNCANNT NNNNNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5'LTR, HIV-1 psi Sequence file: RDB17866_A9Lhd.seq
>D06420A1_B0Cb_3_CMV_Forward_D01_10_ABI24.ab1     1 NNNNNNNNNN NNCNNNNCCN TTGNNGCNAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT    61 ATATAAGCAG CGCGTTTTGC CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG   121 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG   181 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC   241 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG   301 GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG   361 GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA   421 GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA   481 TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA   541 GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC   601 AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT   661 ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG   721 AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GANCACCGCA CAGCAAGGGC   781 CGCTGATCTT CAGANCTGGA NGANGAGATA TGAGGGACAA TTGGAGAAGT GAATTATATA   841 AATATAAAGT AGTAAAAATT GAACCNTTAN GAGTANCACC CNCCAAGGCA AAGAGAANAG   901 TGGTGNAGAN AGAAAAAAGA NCAGNGGGNA TNNNANCTTT GTNNCTTGGG TTCTTGGGNN   961 CAGCNNNAGC ACTNNNGGNG CNNCGNCANT GNNNCNNANN GNACNNNCNN NNNNNTTATT 1021 GNCNNNNNTN NNGCNNCNNC NNANNNNNTN NNNNNNNNN //
Primer: EF1a-N_R (Pr0837) Region: EF1a pro, cPPT_CTS, RRE Sequence file: RDB17866_A9Lhe.seq
>D06420A1_B0Cb_3_EF1a-N_R_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGG CGNNGTGCGC TCTGCCNNCT GACGGGCACC GGAGCCTCAC    61 GAAGCTTGAA TTAATTCAAA GTGAATTTCT GCTGTCCCTG TAATAAACCC GAAAATTTTG   121 AATTTTTGTA ATTTGTTTTT GTAATTCTTT AGTTTGTATG TCTGTTGCTA TTATGTCTAC   181 TATTCTTTCC CCTGCACTGT ACCCCCCAAT CCCCCCTTTT CTTTTAAAAT TGTGGATGAA   241 TACTGCCATT TGTAGATCCG TTCACTAATC GAATGGATCT GTCTCTGTCT CTCTCTCCAC   301 CTTCTTCTTC TATTCCTTCG GGCCTGTCGG GTCCCCTCGG GGTTGGGAGG TGGGTCTGAA   361 ACGATAATGG TGAATATCCC TGCCTAACTC TATTCACTAT AGAAAGTACA GCAAAAACTA   421 TTCTTAAACC TACCAAGCCT CCTACTATCA TTATGAATAA TTTTATATAC CACAGCCAAT   481 TTGTTATGTT AAACCAATTC CACAAACTTG CCCATTTATC TAATTCCAAT AATTCTTGTT   541 CATTCTTTTC TTGCTGGTTT TGCGATTCTT CAATTAAGGA GTGTATTAAG CTTGTGTAAT   601 TGTTAATTTC TCTGTCCCAC TCCATCCAGG TCGTGTGATT CCAAATCTGT TCCAGAGATT   661 TATTACTCCA ACTAGCATTC CAAGGCACAG CAGTGGTGCA AATGAGTTTT CCAGAGCAAC   721 CCCAAATCCC CAGGAGCTGT TGATCCTTTA GGTATCTTTC CACAGCCAGG ATTCTTGCCT   781 GGAGCTGCTT GATGCCCCAG ACTGTGAGTT GCAACAGATG CTGTTGCGCC TCAATAGCCC   841 TCANCAAATT GNTCTGCTGC TGCACTATAC CAGANAATAA TTGTCTGGNC TGTANCGTCA   901 GCGTCNTTGA NNCTGCGCCC ATAGTGCTTC CTGNTGNTCC CAANAACCNN AGGAANAAAG   961 CTCCTATTCC CACTGCTCTT TTTTCTCTCN NNANNNCTNT NCNCNTTGNN TTNNNNGGNN 1021 NTNNNNCNNN NNNNNNCNNT TTTTNNNANT TNANNNTNNN NNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: BGH p(A), 3'LTR, PRE Sequence file: RDB17866_A9Lhf.seq
>D06420A1_B0Cb_3_BGH_rev2_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNGGGGGNN GGGCANCAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA    61 GGCTGATCAG CGGGTTTAAA CGGGCCCTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC   121 TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC   181 AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG   241 CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGTCCGG ATGCAGCTCT CGGGCCATGT   301 GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAATAC TTCTCTCTCC GGGTCATCCA   361 TCCCATGCAG GCTCACAGGG TGTAACAAGC GGGTGTTCTC TCCTTCATTG GCTTCTTCTA   421 CCTTCTCTTG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT GGATATCTGA   481 TCCCTGGCCC TGGTGTGTAG TTCTGCCAAT CAGGGAAGTA GCCTTGTGTG TGGTAGATCC   541 ACAGATCAAG GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT CCCCCCTTTT   601 CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT AAAGGTACCT   661 GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG GCACAATCAG   721 CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG TCTCGACGGT   781 ATCGATGCGG GGANGCGGCC CAAAGGGAGA TCCGACTCGT CTGANGGCGA NNNGAAGANG   841 CGGAAGANGN CGCAGANCCG GCAGCANGCC GCGGGAANGA ANGNCNNNTG GNTTGANGGN   901 CNAANNGNAN GTANCANANN NANGTCCCGC GCANAANNCA NGTGGNANCN CNNGNNANCA   961 NCCANNGAAA NGNANGNCNG CTNNCCCGAN ANCNNNNCNG NNNNGNCNGN GNNNNNNANN 1021 NNANCCNCNN NTCNNNNNCN NNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, Zeo, SV40 p(A) Sequence file: RDB17866_A9Lhg.seq
>D06420A1_B0Cb_3_SV40pro_ori_F_H01_22_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNNC CTCTGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT    61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT   121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA   181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG   241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG   301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG   361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT   421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA   481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT   541 GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG   601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC   661 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT   721 ATAATGGTTA CNAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC   781 TGCATTCTAG TTGTGGTTTG TCCNAACTCA TCAATGTATC TTATCATGTC TGTATACCGT   841 CGANCTCTAG CTAGAGCTTG NCGTANTCAT GNNCATAGCT GTTTCCTGTG TGAAATTGTT   901 ATCCGCTCAC NATTCCNCAC AACATACGAN CCGGAANCNT AAAGNGNAAA GNCNGGGGNG   961 NCTAATGAGT GANCTNNCTC ACATTNANTN GNNTTGNNCT CNNTGNCCNN TTTNCAGNNN  1021 NNAAANNNGN CNNGNNNNNN GNNNNAATGN NNNNNNCNAN NNNNNGGNNN ANNNNNNNNN 1081 N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

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