ATP measurement, tubulin
Alternative name | pN1-Tub beta5-BPDD (stock no. 1088) |
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Clone info. | ATP measurement, tubulin (Tub beta5). CMV promoter. |
Comment | Expression was confirmed by the depositor in mammalian cell. This probe may not localize correctly. |
Vector backbone | pN1 (plasmid) |
Gene/insert name | Aequorea victoria GFP cDNA Mouse Tubb5 cDNA |
Depositor|Developer | Okada, Yasushi | Yaginuma, Hideyuki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | The RECIPIENT acknowledges that the BIOLOGICAL RESOURCE contains the modification of genetically-encoded ratiometric fluorescent ATP indicator QUEEN made by the collaboration of Dr. Hiromi Imamura of the Kyoto University and Dr. Hideyuki Yaginuma of the University of Tokyo and modified by Dr. Hideyuki Yaginuma who moved to RIKEN and Dr. Yasushi Okada of RIKEN. The Kyoto University, the University of Tokyo, and RIKEN are the co-owner of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (Yaginuma, H. and Okada, Y., (2021) bioRxiv https://doi.org/10.1101/2021.10.08.463131) designated by the DEPOSITOR and an acknowledgment to Dr. Yasushi Okada are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research. For for-profit-use of the BIOLOGICAL RESOURCE, the RECIPIENT shall contact the Kyoto University, Accounts Division Administration Office (Science), Main Campus (A20gaibushikin@mail2.adm.kyoto-u.ac.jp), the University of Tokyo Engineering and Information Science and Engineering Office, Finance Department, External Funding Team (t-pat@t-adm.t.u-tokyo.ac.jp) and the RIKEN Kobe Branch, General Affairs Section (kobe-ippr@riken.jp) in advance and should obtain a commercial license from all of them. Neither the Kyoto University, the University of Tokyo nor the RIKEN has obligation to grant a commercial license. RECIPIENT must comply with the terms and conditions of the AUTM Standard Material Transfer Agreement (for Biological Materials). |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 本件リソースは、京都大学の今村博臣博士並びに東京大学の柳沼秀幸博士が共同で開発したアデノシン3リン酸測定のための蛍光標識融合タンパク質QUEENをコードするプラスミドを理化学研究所に移籍した柳沼秀幸博士が岡田康志博士とともに改変した変異体であり、京都大学、東京大学、及び理化学研究所が共有するものである。利用者は、研究成果の公表にあたって寄託者が指定する文献 (Yaginuma, H. and Okada, Y., (2021) bioRxiv https://doi.org/10.1101/2021.10.08.463131) を引用し、謝辞に岡田康志博士を含めること。 本件リソースは非営利機関の学術研究目的にのみ利用することができる。利用者が営利目的利用を希望するときは、京都大学本部構内(理系)共通事務部 経理課 外部資金掛 (A20gaibushikin@mail2.adm.kyoto-u.ac.jp)、 東京大学工学系・情報理工学系等事務部 財務課 外部資金チーム (t-pat@t-adm.t.u-tokyo.ac.jp) 並びに理化学研究所神戸事業所総務課 (kobe-ippr@riken.jp) に連絡し、それぞれから別途の許諾を事前に受けるものとする。尚、京都大学、東京大学並びに理化学研究所はかかる許諾を行う義務を負うものではない。利用者は、AUTM Standard Material Transfer Agreement (for Biological Materials)に定める諸条件を遵守すること。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB17813 | pN1-Tub beta5-QUE37C | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pN1-Tub beta5-QUE37C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17813). |
Reference section:
Yaginuma, H., Okada, Y., Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP indicator protein, QUEEN-37C. bioRxiv https://doi.org/10.1101/2021.10.08.463131 (2021) [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Yaginuma, H., Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP indicator protein, QUEEN-37C. bioRxiv https://doi.org/10.1101/2021.10.08.463131 (2021) [link to RRC of NBRP] |
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