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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pN1-QUE37C (#RDB17794)

ATP measurement

Alternative name pN1-BPDD (stock no. 1042)
Clone info. ATP measurement. CMV promoter.
Comment Expression was confirmed by the depositor in mammalian cell.
Vector backbone pN1 (plasmid)
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Okada, Yasushi | Yaginuma, Hideyuki |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The RECIPIENT acknowledges that the BIOLOGICAL RESOURCE contains the modification of genetically-encoded ratiometric fluorescent ATP indicator QUEEN made by the collaboration of Dr. Hiromi Imamura of the Kyoto University and Dr. Hideyuki Yaginuma of the University of Tokyo and modified by Dr. Hideyuki Yaginuma who moved to RIKEN and Dr. Yasushi Okada of RIKEN. The Kyoto University, the University of Tokyo, and RIKEN are the co-owner of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (Yaginuma, H. and Okada, Y., (2021) bioRxiv https://doi.org/10.1101/2021.10.08.463131) designated by the DEPOSITOR and an acknowledgment to Dr. Yasushi Okada are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research. For for-profit-use of the BIOLOGICAL RESOURCE, the RECIPIENT shall contact the Kyoto University, Accounts Division Administration Office (Science), Main Campus (A20gaibushikin@mail2.adm.kyoto-u.ac.jp), the University of Tokyo Engineering and Information Science and Engineering Office, Finance Department, External Funding Team (t-pat@t-adm.t.u-tokyo.ac.jp) and the RIKEN Kobe Branch, General Affairs Section (kobe-ippr@riken.jp) in advance and should obtain a commercial license from all of them. Neither the Kyoto University, the University of Tokyo nor the RIKEN has obligation to grant a commercial license. RECIPIENT must comply with the terms and conditions of the AUTM Standard Material Transfer Agreement (for Biological Materials).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは、京都大学の今村博臣博士並びに東京大学の柳沼秀幸博士が共同で開発したアデノシン3リン酸測定のための蛍光標識融合タンパク質QUEENをコードするプラスミドを理化学研究所に移籍した柳沼秀幸博士が岡田康志博士とともに改変した変異体であり、京都大学、東京大学、及び理化学研究所が共有するものである。利用者は、研究成果の公表にあたって寄託者が指定する文献 (Yaginuma, H. and Okada, Y., (2021) bioRxiv https://doi.org/10.1101/2021.10.08.463131) を引用し、謝辞に岡田康志博士を含めること。 本件リソースは非営利機関の学術研究目的にのみ利用することができる。利用者が営利目的利用を希望するときは、京都大学本部構内(理系)共通事務部 経理課 外部資金掛 (A20gaibushikin@mail2.adm.kyoto-u.ac.jp)、 東京大学工学系・情報理工学系等事務部 財務課 外部資金チーム (t-pat@t-adm.t.u-tokyo.ac.jp) 並びに理化学研究所神戸事業所総務課 (kobe-ippr@riken.jp) に連絡し、それぞれから別途の許諾を事前に受けるものとする。尚、京都大学、東京大学並びに理化学研究所はかかる許諾を行う義務を負うものではない。利用者は、AUTM Standard Material Transfer Agreement (for Biological Materials)に定める諸条件を遵守すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17794 pN1-QUE37C DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pN1-QUE37C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17794).

Reference section:

Yaginuma, H., Okada, Y., Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP indicator protein, QUEEN-37C. bioRxiv https://doi.org/10.1101/2021.10.08.463131 (2021) [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17794_A9Lap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'
Sequence file: RDB17794_A9Laa.seq check
>D06362H1_A9La_1_CMV_Forward_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTGN NGCNNTGGGC GGTANGCGTG TACGGTGGGA NGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGAGC
  121 TCAAGCTTCG AATTCTGCAG TCGACGGTAC CGCGGGCCCG GGATCCACCG GTCGCCACCA
  181 TGGTGAAAAC TGTGAAAGTG AATATAACAA CCCCTGATGG GCCAGTCTAC GACGCTGATA
  241 TCGAGATGGT GTCCGTGCGG GCCGAGAGTG GTGATCTCGG CATCCTCCCC GGTCACATTC
  301 CCACAAAGGC CCCACTGAAG ATCGGAGCTG TGCGGCTGAA GAAGGACGGC CAAACAGAGG
  361 ATGTCGCAGT CTCAGGCGGC ACTGTTGAAG TGCGGCCTGA CCACGTTACC ATTAATGCTC
  421 AAGCCGCTGA ACGGGCGGAG GACATTGACG TCCTCCGCGC CAAAGCGGCG AAAGAGCGGG
  481 CGGAGCGCCG CCTGCAAAGC CAGACCCGTT ACAACAGCCA CAACGTCTAT ATCATGGCCG
  541 ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA
  601 GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC
  661 TGCCCGACAA CCACTACCTG AGCACCCAGT CCAAGCTGAN CAAAGACCCC AACGAGAAGC
  721 GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG
  781 ANCTGTACAA GGGTGGCACC GGANGGNNCA TGGNGAGCAA GGGCGNNGAN CTGTTCACCG
  841 GGGGNGGNGC CCATCCTGGN CGANCTGGAC GGCGANNNAA ACGGNCNCAA NTNCAGCGNG
  901 NCCNGNNANG NNGANGGCGA TGCCNNNNAC NGNNNNCNNN NCNNNAANTN NNTNNNNNNN
961 NNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17794_A9Lab.seq check
>D06362H1_A9La_1_SV40polyA-R2_C02_08_ABI24.ab1
    1 NNNNNNNNNN CNNCANANNN ANGTTAACAN NNACNATTGC ATTCNTTTTA TGTTTCAGGT
   61 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
  121 TGATTATGAT CTAGAGTCGC GGCCGCTCAC TTATCTTCCG CAACGCTCAA ACGGTTCATG
  181 GCGCGTTTTA ACGCCAGTTC GGCCCGTTTG AAGTCGATGT CACCCAGGTT GTACTCCAGC
  241 TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC
  301 ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG
  361 AAGAAGATGG TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC
  421 TGCTTCATGT GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG
  481 AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG
  541 CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG
  601 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC
  661 ATGCTCCCTC CGGTGCCACC CTTGTACAGC TCGTCCATGC CGANAGTGAT CCCGGCGGCG
  721 GTCACGAACT CCAGCAGGAN CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG
  781 NACTNNNTGC TCANGTAGNG GTTGTCGGGC AGCANCACGG GGCCGTCGCC NANGNNGGTG
  841 TTCTGCTGGT ANNGGTCGGC GANCTGCACG CTGCCNNNCT CNATGNTGTG GCGGATCTNN
901 AAGTTCACCT NNNTGNCGTT CTNNNGCTNN NCNGNNNNNN NNNN
//
Primer: QUEEN-M_R (Pr0808)
Region: insert mid
Sequence file: RDB17794_A9Lac.seq check
>D06362H1_A9La_1_QUEEN-M_R_C07_03_ABI08.ab1
    1 NNNNNNNNNN NNCNNNNNNN NNNNCNNGNN NNGCTGNACT TGTGGCCGTT TACGTCGCCG
   61 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG
  121 CTCCCTCCGG TGCCACCCTT GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGCGGTC
  181 ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGCTTGGAC
  241 TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC
  301 TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG
  361 TTCACCTTGA TGCCGTTCTT CTGCTTGTCG GCCATGATAT AGACGTTGTG GCTGTTGTAA
  421 CGGGTCTGGC TTTGCAGGCG GCGCTCCGCC CGCTCTTTCG CCGCTTTGGC GCGGAGGACG
  481 TCAATGTCCT CCGCCCGTTC AGCGGCTTGA GCATTAATGG TAACGTGGTC AGGCCGCACT
  541 TCAACAGTGC CGCCTGAGAC TGCGACATCC TCTGTTTGGC CGTCCTTCTT CAGCCGCACA
  601 GCTCCGATCT TCAGTGGGGC CTTTGTGGGA ATGTGACCGG GGAGGATGCC GAGATCACCA
  661 CTCTCGGCCC GCACGGACAC CATCTCGATA TCAGCGTCGT AGACTGGCCC ATCAGGGGTT
  721 GTTATATTNN NTTTCACAGT TTTCACCATG GTGGCGACCG GTGGATCCCG GGCCCGCGGT
  781 ACCGTCGACT GCAGAATTCG AAGCTTGAGC TCGAGATCTG AGTCCGGTAN CGCTAGCGGA
  841 TCTGACNGTT CANTANNCCA GCTCTGCTTA TATAGACCTC CCNNCGTANA CGCCTACCGC
  901 CCNTTTGCGT CNATGGNNNG GANTTGTTAC NACATTTTGG AAAGNNCCGT TGANTTTGGT
  961 GNCNAAACAA ACTNNNANNG NNGTNNATGG GGNGGANNNN NNGNAAANNC CCNNGANNNA
1021 NNNNCTNTNC NNNNCCNNNG NNNNANNNNN NAANNNNNNN NNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB17794_A9Lad.seq check
>D06362H1_A9La_1_SV40pro_ori_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNGNNGCCTC NGCCTCTGNN CTATTCNNGA AGTAGTGAGG AGGCTTTTTT
   61 GGAGGCCTAG GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT
  121 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA
  181 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA
  241 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA
  301 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA
  361 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT
  421 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG
  481 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA
  541 GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA
  601 TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC CCGACGGCGA
  661 GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG
  721 CTTTTCTGGA TTCATCGACT GNNNCCGGCT GGGTGTGGCG GANCGCTATC ANGACATAGC
  781 GTTGGCTACC CGTGATATTG CTGANNANCT TGGCGGCGAA TNNNCTGANC GCTTCCTCGN
  841 GCNNNACNGT ATCGCCGCNC CCGATTCNCA NCGCATCGCC TTCTATCGCC NTCNNNANGA
  901 NTNCTTCTNN NCGGGNNNCN NGGGNNNCNA AANGANCNAN CNANNNNCNN CCNANCNNNN
961 NNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yaginuma, H., Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP indicator protein, QUEEN-37C. bioRxiv https://doi.org/10.1101/2021.10.08.463131 (2021) [link to RRC of NBRP]