Resource data sheet
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pH measurement

Catalog number RDB17790
Resource name pN1-pHlu
Alternative name pN1-pHlu (stock no. 1033)
Clone info. pH measurement. CMV promoter.
Comment Expression was confirmed by the depositor in mammalian cell.
Vector backbone pN1 (plasmid)
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Okada, Yasushi | Yaginuma, Hideyuki |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The RECIPIENT acknowledges that the BIOLOGICAL RESOURCE contains the modification of genetically-encoded ratiometric fluorescent ATP indicator QUEEN made by the collaboration of Dr. Hiromi Imamura of the Kyoto University and Dr. Hideyuki Yaginuma of the University of Tokyo and modified by Dr. Hideyuki Yaginuma who moved to RIKEN and Dr. Yasushi Okada of RIKEN. The Kyoto University, the University of Tokyo, and RIKEN are the co-owner of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (Manuscript is in preparation and announced after publication) designated by the DEPOSITOR and an acknowledgment to Dr. Yasushi Okada are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research. For for-profit-use of the BIOLOGICAL RESOURCE, the RECIPIENT shall contact the Kyoto University, Accounts Division Administration Office (Science), Main Campus (, the University of Tokyo Engineering and Information Science and Engineering Office, Finance Department, External Funding Team ( and the RIKEN Kobe Branch, General Affairs Section ( in advance and should obtain a commercial license from all of them. Neither the Kyoto University, the University of Tokyo nor the RIKEN has obligation to grant a commercial license. RECIPIENT must comply with the terms and conditions of the AUTM Standard Material Transfer Agreement (for Biological Materials).
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            提供条件 本件リソースは、京都大学の今村博臣博士並びに東京大学の柳沼秀幸博士が共同で開発したアデノシン3リン酸測定のための蛍光標識融合タンパク質QUEENをコードするプラスミドを理化学研究所に移籍した柳沼秀幸博士が岡田康志博士とともに改変した変異体であり、京都大学、東京大学、及び理化学研究所が共有するものである。利用者は、研究成果の公表にあたって寄託者が指定する文献(投稿準備中につき、出版後に追加する) を引用し、謝辞に岡田康志博士を含めること。 本件リソースは非営利機関の学術研究目的にのみ利用することができる。利用者が営利目的利用を希望するときは、京都大学本部構内(理系)共通事務部 経理課 外部資金掛 (、 東京大学工学系・情報理工学系等事務部 財務課 外部資金チーム ( 並びに理化学研究所神戸事業所総務課 ( に連絡し、それぞれから別途の許諾を事前に受けるものとする。尚、京都大学、東京大学並びに理化学研究所はかかる許諾を行う義務を負うものではない。利用者は、AUTM Standard Material Transfer Agreement (for Biological Materials)に定める諸条件を遵守すること。
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            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB17790 pN1-pHlu Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pN1-pHlu was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17790).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            Original, user report and related articles

            reference Miesenböck, G., Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394 (6689): 192-195 (1998). PMID 9671304.