RDB17788 - pRSAN-QUE37C

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pRSAN-QUE37C (#RDB17788)

ATP measurement

Alternative name	pRSAN-BPDD (stock no. 1029)
Clone info.	ATP measurement. T7 promoter.
Comment	Expression was confirmed by the depositor in E coli.
Vector backbone	pRSAN (plasmid)
Gene/insert name	Aequorea victoria GFP cDNA
Depositor\|Developer	Okada, Yasushi \| Yaginuma, Hideyuki \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution The RECIPIENT acknowledges that the BIOLOGICAL RESOURCE contains the modification of genetically-encoded ratiometric fluorescent ATP indicator QUEEN made by the collaboration of Dr. Hiromi Imamura of the Kyoto University and Dr. Hideyuki Yaginuma of the University of Tokyo and modified by Dr. Hideyuki Yaginuma who moved to RIKEN and Dr. Yasushi Okada of RIKEN. The Kyoto University, the University of Tokyo, and RIKEN are the co-owner of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (Yaginuma, H. and Okada, Y., (2021) bioRxiv https://doi.org/10.1101/2021.10.08.463131) designated by the DEPOSITOR and an acknowledgment to Dr. Yasushi Okada are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research. For for-profit-use of the BIOLOGICAL RESOURCE, the RECIPIENT shall contact the Kyoto University, Accounts Division Administration Office (Science), Main Campus (A20gaibushikin@mail2.adm.kyoto-u.ac.jp), the University of Tokyo Engineering and Information Science and Engineering Office, Finance Department, External Funding Team (t-pat@t-adm.t.u-tokyo.ac.jp) and the RIKEN Kobe Branch, General Affairs Section (kobe-ippr@riken.jp) in advance and should obtain a commercial license from all of them. Neither the Kyoto University, the University of Tokyo nor the RIKEN has obligation to grant a commercial license. RECIPIENT must comply with the terms and conditions of the AUTM Standard Material Transfer Agreement (for Biological Materials).

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The RECIPIENT acknowledges that the BIOLOGICAL RESOURCE contains the modification of genetically-encoded ratiometric fluorescent ATP indicator QUEEN made by the collaboration of Dr. Hiromi Imamura of the Kyoto University and Dr. Hideyuki Yaginuma of the University of Tokyo and modified by Dr. Hideyuki Yaginuma who moved to RIKEN and Dr. Yasushi Okada of RIKEN. The Kyoto University, the University of Tokyo, and RIKEN are the co-owner of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (Yaginuma, H. and Okada, Y., (2021) bioRxiv https://doi.org/10.1101/2021.10.08.463131) designated by the DEPOSITOR and an acknowledgment to Dr. Yasushi Okada are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research. For for-profit-use of the BIOLOGICAL RESOURCE, the RECIPIENT shall contact the Kyoto University, Accounts Division Administration Office (Science), Main Campus (A20gaibushikin@mail2.adm.kyoto-u.ac.jp), the University of Tokyo Engineering and Information Science and Engineering Office, Finance Department, External Funding Team (t-pat@t-adm.t.u-tokyo.ac.jp) and the RIKEN Kobe Branch, General Affairs Section (kobe-ippr@riken.jp) in advance and should obtain a commercial license from all of them. Neither the Kyoto University, the University of Tokyo nor the RIKEN has obligation to grant a commercial license. RECIPIENT must comply with the terms and conditions of the AUTM Standard Material Transfer Agreement (for Biological Materials).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースは、京都大学の今村博臣博士並びに東京大学の柳沼秀幸博士が共同で開発したアデノシン3リン酸測定のための蛍光標識融合タンパク質QUEENをコードするプラスミドを理化学研究所に移籍した柳沼秀幸博士が岡田康志博士とともに改変した変異体であり、京都大学、東京大学、及び理化学研究所が共有するものである。利用者は、研究成果の公表にあたって寄託者が指定する文献 (Yaginuma, H. and Okada, Y., (2021) bioRxiv https://doi.org/10.1101/2021.10.08.463131) を引用し、謝辞に岡田康志博士を含めること。本件リソースは非営利機関の学術研究目的にのみ利用することができる。利用者が営利目的利用を希望するときは、京都大学本部構内（理系）共通事務部経理課外部資金掛 (A20gaibushikin@mail2.adm.kyoto-u.ac.jp)、東京大学工学系・情報理工学系等事務部財務課外部資金チーム (t-pat@t-adm.t.u-tokyo.ac.jp) 並びに理化学研究所神戸事業所総務課 (kobe-ippr@riken.jp) に連絡し、それぞれから別途の許諾を事前に受けるものとする。尚、京都大学、東京大学並びに理化学研究所はかかる許諾を行う義務を負うものではない。利用者は、AUTM Standard Material Transfer Agreement (for Biological Materials)に定める諸条件を遵守すること。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

本件リソースは、京都大学の今村博臣博士並びに東京大学の柳沼秀幸博士が共同で開発したアデノシン3リン酸測定のための蛍光標識融合タンパク質QUEENをコードするプラスミドを理化学研究所に移籍した柳沼秀幸博士が岡田康志博士とともに改変した変異体であり、京都大学、東京大学、及び理化学研究所が共有するものである。利用者は、研究成果の公表にあたって寄託者が指定する文献 (Yaginuma, H. and Okada, Y., (2021) bioRxiv https://doi.org/10.1101/2021.10.08.463131) を引用し、謝辞に岡田康志博士を含めること。本件リソースは非営利機関の学術研究目的にのみ利用することができる。利用者が営利目的利用を希望するときは、京都大学本部構内（理系）共通事務部経理課外部資金掛 (A20gaibushikin@mail2.adm.kyoto-u.ac.jp)、東京大学工学系・情報理工学系等事務部財務課外部資金チーム (t-pat@t-adm.t.u-tokyo.ac.jp) 並びに理化学研究所神戸事業所総務課 (kobe-ippr@riken.jp) に連絡し、それぞれから別途の許諾を事前に受けるものとする。尚、京都大学、東京大学並びに理化学研究所はかかる許諾を行う義務を負うものではない。利用者は、AUTM Standard Material Transfer Agreement (for Biological Materials)に定める諸条件を遵守すること。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB17788	pRSAN-QUE37C	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pRSAN-QUE37C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17788).

Reference section:

Yaginuma, H., Okada, Y., Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP indicator protein, QUEEN-37C. bioRxiv https://doi.org/10.1101/2021.10.08.463131 (2021) [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17788_A9Lap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Qeen-M_R (Pr1290) Region: insert 5',Xpress tag,T7 tag Sequence file: RDB17788_A9Laa.seq
>17788_17788_B3B3F_1_Qeen-M_R_G02_20_ABI24.ab1 1 NNNNNNNNNN NNNCCCCNGT GANNGCTCCT CGCCCTTGCT CNCCNTGCTC CCTCCGGTGC 61 CACCCTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG AACTCCAGCA 121 GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG CTTGGACTGG GTGCTCAGGT 181 AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC TGGTAGTGGT 241 CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC ACCTTGATGC 301 CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAACGG GTCTGGCTTT 361 GCAGGCGGCG CTCCGCCCGC TCTTTCGCCG CTTTGGCGCG GAGGACGTCA ATGTCCTCCG 421 CCCGTTCAGC GGCTTGAGCA TTAATGGTAA CGTGGTCAGG CCGCACTTCA ACAGTGCCGC 481 CTGAGACTGC GACATCCTCT GTTTGGCCGT CCTTCTTCAG CCGCACAGCT CCGATCTTCA 541 GTGGGGCCTT TGTGGGAATG TGACCGGGGA GGATGCCGAG ATCACCACTC TCGGCCCGCA 601 CGGACACCAT CTCGATATCA GCGTCGTAGA CTGGCCCATC AGGGGTTGTT ATATTCACTT 661 TCACAGTTTT CACCATGGTG GCGACCGGTG GATCCTTATC GTCATCGTCG TACAGATCCC 721 GACCCATTTG CTGTCCACCA GTCATGCTAG CCNTACCATG ATGATGATGA TGATGAGAAC 781 CCCGCATATG TATATCTCCT TCTTAAAGTT AAACNAAATT ATTTCTAGAG GGAAACCGTT 841 GTGNTCTNCN NATAGTGAGT NGNANTNATT TCGCGGNNNN NANNTNNNNN NTTNATGNNT 901 CNNNNNNNNC NNNNGNNNAN NNNGNNTNNN NNNNNNN //
Primer: T7 terminator (Pr0155) Region: insert 3' Sequence file: RDB17788_A9Lab.seq
>17788_17788_B3B3F_1_T7terminator_F02_17_ABI24.ab1 1 NNNNNNNNCN GNNNAAGCTT ATCTAGAGTC GCGGCCGCTC ACTTATCTTC CGCAACGCTC 61 AAACGGTTCA TGGCGCGTTT TAACGCCAGT TCGGCCCGTT TGAAGTCGAT GTCACCCAGG 121 TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC 181 TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG 241 CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG 301 AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA AGCACTGCAC GCCGTAGGTC 361 AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATGAACTTC 421 AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG 481 CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG 541 CCCTTGCTCA CCATGCTCCC TCCGGTGCCA CCCTTGTACA GCTCGTCCAT GCCGAGAGTG 601 ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT 661 TTGCTCAGCT TGGACTGGGT GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG 721 CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCNATGTNG 781 TGGCGGANCT TGAAGTTCAC CTTNNTGCCG TTCTTCTGCT TGTCNGCCAT GANATAGACG 841 TTGTGNCTGN TGNNACNGGT CTGGNTTNNC NNNNGGNGCT NCNNCCGCNN TTNNGNNGCT 901 TNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Yaginuma, H., Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP indicator protein, QUEEN-37C. bioRxiv https://doi.org/10.1101/2021.10.08.463131 (2021) [link to RRC of NBRP]

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External Database
Aequorea victoria GFP

Reference sequence