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pT2MP.EF-pHlu

pH measurement, Tol2 transposon

Catalog number RDB17787
Resource name pT2MP.EF-pHlu
Alternative name pT2MPEF-pHlu (stock no. 1019)
Clone info. pH measurement, Tol2 transposon. EF promoter.
Comment Expression was confirmed by the depositor in mammalian cell.
Vector backbone pT2+MCS (plasmid)
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Okada, Yasushi | Yaginuma, Hideyuki |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The RECIPIENT acknowledges that the BIOLOGICAL RESOURCE contains the modification of genetically-encoded ratiometric fluorescent ATP indicator QUEEN made by the collaboration of Dr. Hiromi Imamura of the Kyoto University and Dr. Hideyuki Yaginuma of the University of Tokyo and modified by Dr. Hideyuki Yaginuma who moved to RIKEN and Dr. Yasushi Okada of RIKEN. The Kyoto University, the University of Tokyo, and RIKEN are the co-owner of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (Manuscript is in preparation and announced after publication) designated by the DEPOSITOR and an acknowledgment to Dr. Yasushi Okada are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research. For for-profit-use of the BIOLOGICAL RESOURCE, the RECIPIENT shall contact the Kyoto University, Accounts Division Administration Office (Science), Main Campus (A20gaibushikin@mail2.adm.kyoto-u.ac.jp), the University of Tokyo Engineering and Information Science and Engineering Office, Finance Department, External Funding Team (t-pat@t-adm.t.u-tokyo.ac.jp) and the RIKEN Kobe Branch, General Affairs Section (kobe-ippr@riken.jp) in advance and should obtain a commercial license from all of them. Neither the Kyoto University, the University of Tokyo nor the RIKEN has obligation to grant a commercial license. RECIPIENT must comply with the terms and conditions of the AUTM Standard Material Transfer Agreement (for Biological Materials).
            Additional terms and conditions for distribution The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 本件リソースは、京都大学の今村博臣博士並びに東京大学の柳沼秀幸博士が共同で開発したアデノシン3リン酸測定のための蛍光標識融合タンパク質QUEENをコードするプラスミドを理化学研究所に移籍した柳沼秀幸博士が岡田康志博士とともに改変した変異体であり、京都大学、東京大学、及び理化学研究所が共有するものである。利用者は、研究成果の公表にあたって寄託者が指定する文献(投稿準備中につき、出版後に追加する) を引用し、謝辞に岡田康志博士を含めること。 本件リソースは非営利機関の学術研究目的にのみ利用することができる。利用者が営利目的利用を希望するときは、京都大学本部構内(理系)共通事務部 経理課 外部資金掛 (A20gaibushikin@mail2.adm.kyoto-u.ac.jp)、 東京大学工学系・情報理工学系等事務部 財務課 外部資金チーム (t-pat@t-adm.t.u-tokyo.ac.jp) 並びに理化学研究所神戸事業所総務課 (kobe-ippr@riken.jp) に連絡し、それぞれから別途の許諾を事前に受けるものとする。尚、京都大学、東京大学並びに理化学研究所はかかる許諾を行う義務を負うものではない。利用者は、AUTM Standard Material Transfer Agreement (for Biological Materials)に定める諸条件を遵守すること。
            付加的提供条件 Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所 川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB17787 pT2MP.EF-pHlu Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Original reference

            original Yaginuma, H., Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP indicator protein, QUEEN-37C. bioRxiv https://doi.org/10.1101/2021.10.08.463131 (2021)

            Further references such as user reports and related articles (go to bottom)

            Featured content


            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Yaginuma, H., Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP indicator protein, QUEEN-37C. bioRxiv https://doi.org/10.1101/2021.10.08.463131 (2021)

            2022.05.17

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