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pT2MP.EF-NLS-Q2m

QUEEN ATP sensor for 25degC, nucleus, Tol2 transposon

Catalog number RDB17785
Resource name pT2MP.EF-NLS-Q2m
Alternative name pT2MPEF-NLS-Q2m (stock no. 1113)
Clone info. QUEEN ATP sensor for 25degC, nucleus (NLS), Tol2 transposon. EF promoter.
Comment Expression was confirmed by the depositor in mammalian cell.
Vector backbone pT2+MCS (plasmid)
Gene/insert name Aequorea victoria GFP cDNA Bacillus sp. PS3 F0F1-ATP synthase epsilon subunit genomic DNA Bacillus subtilis F0F1-ATP synthase epsilon subunit genomic DNA Synthetic NLS
Depositor|Developer Yaginuma, Hideyuki | Imamura, Hiromi |
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External Database
Aequorea victoria GFP
    Bacillus sp. PS3 F0F1-ATP synthase epsilon subunit Bacillus subtilis F0F1-ATP synthase epsilon subunit Synthetic NLS

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR The RECIPIENT acknowledges that the BIOLOGICAL RESOURCE contains the genetically-encoded ratiometric fluorescent ATP indicator QUEEN made by the collaboration of Dr. Hiromi Imamura of the Kyoto University and Dr. Hideyuki Yaginuma of the University of Tokyo. Both the Kyoto University and the University of Tokyo are the co-owner of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (Yaginuma, H. et al., Sci. Rep. 4: 6522, 2014) designated by the DEPOSITORs and an acknowledgment to Dr. Hiromi Imamura are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research. For for-profit-use of the BIOLOGICAL RESOURCE, the RECIPIENT shall contact both the Kyoto University, Accounts Division Administration Office (Science), Main Campus (A20gaibushikin@mail2.adm.kyoto-u.ac.jp) and the University of Tokyo Engineering and Information Science and Engineering Office, Finance Department, External Funding Team (t-pat@t-adm.t.u-tokyo.ac.jp) in advance and should obtain a commercial license from both Universities. Neither the Kyoto University nor the University of Tokyo has obligation to grant a commercial license. RECIPIENT must comply with the terms and conditions of the AUTM Standard Material Transfer Agreement (for Biological Materials)
        Additional terms and conditions for distribution The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        提供案内 (日本国内) [open/close]

        提供条件 本件リソースは、京都大学の今村博臣博士並びに東京大学の柳沼秀幸博士が共同で開発したアデノシン3リン酸測定のための蛍光標識融合タンパク質QUEENをコードするプラスミドであり、京都大学並びに東京大学が共有するものである。利用者は、研究成果の公表にあたって寄託者が指定する文献 (Yaginuma, H. et al., Sci. Rep. 4: 6522, 2014) を引用し、謝辞に今村博臣博士を含めること。本件リソースは非営利機関の学術研究目的にのみ利用することができる。 利用者が営利目的利用を希望するときは、京都大学本部構内(理系)共通事務部 経理課 外部資金掛 (A20gaibushikin@mail2.adm.kyoto-u.ac.jp) 並びに東京大学工学系・情報理工学系等事務部 財務課 外部資金チーム (t-pat@t-adm.t.u-tokyo.ac.jp) に連絡し、両大学から別途の許諾を事前に受けるものとする。尚、京都大学並びに東京大学はかかる許諾を行う義務を負うものではない。利用者は、AUTM Standard Material Transfer Agreement (for Biological Materials)に定める諸条件を遵守すること。
        付加的提供条件 Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所 川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

        Catalog # Resource name Availability Shipping form Fee (non-profit org.)
        RDB17785 pT2MP.EF-NLS-Q2m Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References and tips

        Electronic file

        Original reference

        original Yaginuma, H., Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging. Sci. Rep. 4: 6522 (2014). PMID 25283467.

        Further references such as user reports and related articles (go to bottom)

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        Sequence information

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


        References

        Original, user report and related articles

        original Yaginuma, H., Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging. Sci. Rep. 4: 6522 (2014). PMID 25283467.

        2022.07.13

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