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pN1-QUE2m (#RDB17767)

QUEEN ATP sensor for 25degC

Alternative name pN1-QUEEN2 (stock no. 1025)
Clone info. QUEEN ATP sensor for 25degC. CMV promoter.
Comment Expression was confirmed by the depositor in mammalian cell.
Vector backbone pN1 (plasmid)
Gene/insert name Aequorea victoria GFP cDNA Bacillus sp. PS3 F0F1-ATP synthase epsilon subunit genomic DNA Bacillus subtilis F0F1-ATP synthase epsilon subunit genomic DNA
Depositor|Developer Okada, Yasushi | Yaginuma, Hideyuki | Imamura, Hiromi |
 

Distribution information

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Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The RECIPIENT acknowledges that the BIOLOGICAL RESOURCE contains the genetically-encoded ratiometric fluorescent ATP indicator QUEEN made by the collaboration of Dr. Hiromi Imamura of the Kyoto University and Dr. Hideyuki Yaginuma of the University of Tokyo. Both the Kyoto University and the University of Tokyo are the co-owner of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (Yaginuma, H. et al., Sci. Rep. 4: 6522, 2014) designated by the DEPOSITORs and an acknowledgment to Dr. Hiromi Imamura are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research. For for-profit-use of the BIOLOGICAL RESOURCE, the RECIPIENT shall contact both the Kyoto University, Accounts Division Administration Office (Science), Main Campus (A20gaibushikin@mail2.adm.kyoto-u.ac.jp) and the University of Tokyo Engineering and Information Science and Engineering Office, Finance Department, External Funding Team (t-pat@t-adm.t.u-tokyo.ac.jp) in advance and should obtain a commercial license from both Universities. Neither the Kyoto University nor the University of Tokyo has obligation to grant a commercial license. RECIPIENT must comply with the terms and conditions of the AUTM Standard Material Transfer Agreement (for Biological Materials)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは、京都大学の今村博臣博士並びに東京大学の柳沼秀幸博士が共同で開発したアデノシン3リン酸測定のための蛍光標識融合タンパク質QUEENをコードするプラスミドであり、京都大学並びに東京大学が共有するものである。利用者は、研究成果の公表にあたって寄託者が指定する文献 (Yaginuma, H. et al., Sci. Rep. 4: 6522, 2014) を引用し、謝辞に今村博臣博士を含めること。本件リソースは非営利機関の学術研究目的にのみ利用することができる。 利用者が営利目的利用を希望するときは、京都大学本部構内（理系）共通事務部 経理課 外部資金掛 (A20gaibushikin@mail2.adm.kyoto-u.ac.jp) 並びに東京大学工学系・情報理工学系等事務部 財務課 外部資金チーム (t-pat@t-adm.t.u-tokyo.ac.jp) に連絡し、両大学から別途の許諾を事前に受けるものとする。尚、京都大学並びに東京大学はかかる許諾を行う義務を負うものではない。利用者は、AUTM Standard Material Transfer Agreement (for Biological Materials)に定める諸条件を遵守すること。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17767 pN1-QUE2m Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pN1-QUE2m was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17767).

Reference section:

Yaginuma, H., Kawai, S., Tabata, K.V., Tomiyama, K., Kakizuka, A., Komatsuzaki, T., Noji, H., Imamura, H., Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging. Sci. Rep. 4: 6522 (2014). PMID 25283467. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Yaginuma, H., Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging. Sci. Rep. 4: 6522 (2014). PMID 25283467. [link to RRC of NBRP]