RDB17767 - pN1-QUE2m

Catalog number	RDB17767
Resource name	pN1-QUE2m
Alternative name	pN1-QUEEN2 (stock no. 1025)
Clone info.	QUEEN ATP sensor for 25degC. CMV promoter. Expression was confirmed by the depositor in mammalian cell.
Vector backbone	pN1 (plasmid)
Gene/insert name	Aequorea victoria GFP cDNA Bacillus sp. PS3 F0F1-ATP synthase epsilon subunit genomic DNA Bacillus subtilis F0F1-ATP synthase epsilon subunit genomic DNA
Depositor\|Developer	Okada, Yasushi \| Yaginuma, Hideyuki \| Imamura, Hiromi \|

Other clones in our bank	Bacillus sp. PS3 (KEGG orthology K02114) \| Bacillus subtilis (KEGG orthology K02114) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The RECIPIENT acknowledges that the BIOLOGICAL RESOURCE contains the genetically-encoded ratiometric fluorescent ATP indicator QUEEN made by the collaboration of Dr. Hiromi Imamura of the Kyoto University and Dr. Hideyuki Yaginuma of the University of Tokyo. Both the Kyoto University and the University of Tokyo are the co-owner of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (Yaginuma, H. et al., Sci. Rep. 4: 6522, 2014) designated by the DEPOSITORs and an acknowledgment to Dr. Hiromi Imamura are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research. For for-profit-use of the BIOLOGICAL RESOURCE, the RECIPIENT shall contact both the Kyoto University, Accounts Division Administration Office (Science), Main Campus (A20gaibushikin@mail2.adm.kyoto-u.ac.jp) and the University of Tokyo Engineering and Information Science and Engineering Office, Finance Department, External Funding Team (t-pat@t-adm.t.u-tokyo.ac.jp) in advance and should obtain a commercial license from both Universities. Neither the Kyoto University nor the University of Tokyo has obligation to grant a commercial license. RECIPIENT must comply with the terms and conditions of the AUTM Standard Material Transfer Agreement (for Biological Materials)
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users)

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提供条件	本件リソースは、京都大学の今村博臣博士並びに東京大学の柳沼秀幸博士が共同で開発したアデノシン3リン酸測定のための蛍光標識融合タンパク質QUEENをコードするプラスミドであり、京都大学並びに東京大学が共有するものである。利用者は、研究成果の公表にあたって寄託者が指定する文献 (Yaginuma, H. et al., Sci. Rep. 4: 6522, 2014) を引用し、謝辞に今村博臣博士を含めること。本件リソースは非営利機関の学術研究目的にのみ利用することができる。利用者が営利目的利用を希望するときは、京都大学本部構内（理系）共通事務部経理課外部資金掛 (A20gaibushikin@mail2.adm.kyoto-u.ac.jp) 並びに東京大学工学系・情報理工学系等事務部財務課外部資金チーム (t-pat@t-adm.t.u-tokyo.ac.jp) に連絡し、両大学から別途の許諾を事前に受けるものとする。尚、京都大学並びに東京大学はかかる許諾を行う義務を負うものではない。利用者は、AUTM Standard Material Transfer Agreement (for Biological Materials)に定める諸条件を遵守すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

References and tips

Sequence information

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB17767	pN1-QUE2m	Under QC test. Please contact us.	DNA solution

original	Yaginuma, H., Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging. Sci. Rep. 4: 6522 (2014). PMID 25283467.

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.