Resource data sheet
DNA Bank Top

CSIV-TRE-HPV-E6/E7-UbC-KT (#RDB17719)

Lentivirus vector HPV16 E6 and E7 with Tet-responsive promoter.


Drawn by SnapGene® software
Sequence (full) RDB17719hts02.seq
Publication Kurita, R., PLoS One 8 (3): e59890 (2013). [link to RRC of NBRP]
Test sheet RDB17719_B0G1p1-2.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Lentivirus vector construct of transforming protein E6 and E7 region of human papillomavirus type 16 (HPV16) driven by Tet-responsive promoter and hKO1-2A-rtTA marker driven by UbC promoter.
Comment The HPV16-E6/E7 genomic DNA subcloned into pENTR201 was a kind gift of Dr. Kiyono (National Cancer Center Research Institute, Japan). Expression was confirmed by the depositor with flowcytometer or fluorescence microscopy. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSIV-TRE-RfA-UbC-KT (RDB12878) (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions LB+Ampicillin, 37oC
Growth remarks Use host strain suitable for plasmid clones containing repeat elements and unstable inserts.
Gene/insert name Hum_Papillomavirus E6 (HPV16gp1) genomic DNA Hum_Papillomavirus E7 (HPV16gp2) genomic DNA
Depositor|Developer Nakamura, Yukio |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kurita R. et al. PLos One, 8(3): e59890, 2013)
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kurita R. et al. PLos One, 8(3): e59890, 2013)。
付加的使用条件：
本件研究材料は、非営利機関の非営利学術研究に限って提供する。
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17719 CSIV-TRE-HPV-E6/E7-UbC-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSIV-TRE-HPV-E6/E7-UbC-KT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17719).

Reference section:

Kurita, R., Suda, N., Sudo, K., Miharada, K., Hiroyama, T., Miyoshi, H., Tani, K., Nakamura, Y., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656. [link to RRC of NBRP]
reference Trakarnsanga, K., An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells. Nat Commun. 2017 Mar 14;8:14750 (2017). PMID 28290447.