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CSIV-TRE-HPV-E6/E7-UbC-KT

Lentivirus vector HPV16 E6 and E7 with Tet-responsive promoter.

Catalog number RDB17719
Resource name CSIV-TRE-HPV-E6/E7-UbC-KT
Clone info. Lentivirus vector construct of transforming protein E6 and E7 region of human papillomavirus type 16 (HPV16) driven by Tet-responsive promoter and hKO1-2A-rtTA marker driven by UbC promoter.
The HPV16-E6/E7 genomic DNA subcloned into pENTR201 was a kind gift of Dr. Kiyono (National Cancer Center Research Institute, Japan). Expression was confirmed by the depositor with flowcytometer or fluorescence microscopy. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-TRE-RfA-UbC-KT (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Growth remarks Use host strain suitable for plasmid clones containing repeat elements and unstable inserts.
Gene/insert name Hum_Papillomavirus E6 (HPV16gp1) genomic DNA Hum_Papillomavirus E7 (HPV16gp2) genomic DNA
Depositor|Developer Nakamura, Yukio |
 
Other clones in our bank Hum_Papillomavirus E6 (KEGG orthology K21807) | Hum_Papillomavirus E7 (KEGG orthology K21809) |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kurita R. et al. PLos One, 8(3): e59890, 2013)
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering Please visit Ordering instruction.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17719 CSIV-TRE-HPV-E6/E7-UbC-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) provided by the depositor RDB17719pka01.seq

Original reference

original Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17719_A9L9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_v2_F
Region: tight TRE pro (7xtetO), attB1, insert 5'
Sequence file: RDB17719_A9L9h.seq check
>D06794A2-1_A9L9_cPPT+CTS_v2_F_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGA NTACNAAAAC NAATTACAAA AATTCNAAAT TTTCGGGTTT
   61 ATTACAGGGN CAGCAGAAAT TCACTTTGAA TTCGGCCCTT TCGTCTTCAA TCGAGTTTAC
  121 TCCCTATCAG TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGAT
  181 GTCGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA
  241 TAGAGAACGT ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGTTTATC
  301 CCTATCAGTG ATAGAGAACG TATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG
  361 TCGAGGTAGG CGTGTACGGT GGGAGGCCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA
  421 GATCGCCGCT AGCATCACAA GTTTGTACAA AAAAGCAGGC TCCACCATGT TTCAGGACCC
  481 ACAGGAGCGA CCCAGAAAGT TACCACAGTT ATGCACAGAG CTGCAAACAA CTATACATGA
  541 TATAATATTA GAATGTGTGT ACTGCAAGCA ACAGTTACTG CGACGTGAGG TATATGACTT
  601 TGCTTTTCGG GATTTATGCA TAGTATATAG AGATGGGAAT CCATATGCTG TATGTGATAA
  661 ATGTTTAAAG TTTTATTCTA AAATTAGTGA GTATAGACAT TATTGTTATA GTTTGTATGG
  721 AACAACATTA GAACAGCAAT ACAACAAACC GTTGTGTGAT TTGTTAATTA NGTGTATTAA
  781 CTGTCAAAAG CCACTGTNNC CTGAAGAAAA GCAAANNNAT CTGGACNAAA AGCNANNNTT
  841 CCATAATATA AGGNGTCGGN GGANNNNNCN ATGTATGTCT TGNTGCANNT CATCAAGAAC
901 NCNNNNAGAA ACCNANCNNN NNNCANNNNN GNNNNTACAC NNACNTNNNN NNNNNN
//
Primer: UbC-N_R
Region: UbC pro, attB2, insert 3'
Sequence file: RDB17719_A9L9i.seq check
>D06794A2-1_A9L9_UbC-N_R_B07_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNNN NTCCGGGCGG ANGNNCAGGN NGCTCGCTGC
   61 GCCCTTCGTC TGACGTGGCA GCGCTCGCCG TGNGGAGGGG GGCGCCCGCG GGGGGCGCCA
  121 AAACCCGGCG CGGANGCCAG ATCTCCCGAT CTCGAGACCG GTAGCATCAC CACTTTGTAC
  181 AAGAAAGCTG GGTTATGGTT TCTGAGAACA GATGGGGCAC ACAATTCCTA GTGTGCCCAT
  241 TAACAGGTCT TCCAAAGTAC GAATGTCTAC GTGTGTGCTT TGTACGCACA ACCGAAGCGT
  301 AGAGTCACAC TTGCAACAAA AGGTTACAAT ATTGTAATGG GCTCTGTCCG GTTCTGCTTG
  361 TCCAGCTGGA CCATCTATTT CATCCTCCTC CTCTGAGCTG TCATTTAATT GCTCATAACA
  421 GTAGAGATCA GTTGTCTCTG GTTGCAAATC TAACATATAT TCATGCAATG TAGGTGTATC
  481 TCCATGCATG ATTACAGCTG GGTTTCTCTA CGTGTTCTTG ATGATCTGCA ACAAGACATA
  541 CATCGACCGG TCCACCGACC CCTTATATTA TGGAATCTTT GCTTTTTGTC CAGATGTCTT
  601 TGCTTTTCTT CAGGACACAG TGGCTTTTGA CAGTTAATAC ACCTAATTAA CAAATCACAC
  661 AACGGTTTGT TGTATTGCTG TTCTAATGTT GTTCCATACN AACTATNACA ATAATGTCTA
  721 TACTCACTAA TTTTAGAATA AAACTTTANA CATTTATCAC ATACAGCATA TGGATTCCCA
  781 TCTCTATATA CTATGNATAA ATCNCGAAAA GCAAANTCAT ATACNNCACG TCNCANNAAC
  841 TGNTGCNNGC ANTACACACN TNCTAANATT ATATCANGNA TAGTNGTTNG CANCTCTNNN
901 NATAACNNNN NNNNNN
//
Primer: UbCpro_F
Region: hKO 5'
Sequence file: RDB17719_A9L9j.seq check
>D06794A2-1_A9L9_UbCpro_F_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG NNNNNGTCCG CTAAATTCTG GCCGTTTTTG
   61 GCTTTTTTGT TAGANCGGAC CGTGTTTAGC GCTACCGGTC CACCATGGTG AGCGTGATCA
  121 AGCCCGAGAT GAAGATGAAG TACTTCATGG ACGGCAGCGT GAACGGCCAC GAGTTCACCG
  181 TGGAGGGCGA GGGCACCGGC AAGCCCTACG AGGGCCACCA GGAGATGACC CTGAGGGTGA
  241 CAATGGCCAA GGGCGGCCCC ATGCCCTTCA GCTTCGACCT GGTGAGCCAC ACCTTCTGCT
  301 ACGGCCACAG GCCCTTCACC AAGTACCCCG AGGAGATCCC CGACTACTTC AAGCAGGCCT
  361 TCCCCGAGGG CCTGAGCTGG GAGAGGAGCC TCCAGTTCGA GGACGGCGGC TTCGCCGCCG
  421 TGAGCGCCCA CATCAGCCTG AGGGGCAACT GCTTCGAGCA CAAGAGCAAG TTCGTGGGCG
  481 TGAACTTCCC CGCCGACGGC CCCGTGATGC AGAACCAGAG CAGCGACTGG GAGCCCAGCA
  541 CCGAGAAGAT CACCACCTGC GACGGCGTGC TGAAGGGCGA CGTGACCATG TACCTGAAGC
  601 TGGCCGGCGG CGGCAACCAC AAGTGCCAGT TCAAGACCAC CTACAAGGCC GCCAAGAAGA
  661 TCCTGAAGAT GCCCCAGAGC CACTTCATCG GCCACAGGCT GGTGANGAAG ANCGANGGCA
  721 ACATCACCGA GCTGGTGGAN GANGCCGTGG CCCACTGCGN NGGCAGANGA AGTCTTCTAA
  781 CATGCGGTGA NGTGNANGAN AATCNNGGCC CTATGTCTAG ACTGGNCAAN ANCAAAGNCA
  841 TAAACGGNGC TCNGGAATTA CTCANNNGAN NNNGNATCNN ANNNCCNNNN GANNNNGAAA
901 NNCGCNCAAA ANNNGGNANT NNANCANNNN ANCNNNNNNN NNN
//
Primer: mKO2-M_F
Region: hKO 3', T2A, rtTA-Advenced
Sequence file: RDB17719_A9L9k.seq check
>D06794A2-1_A9L9_mKO2-M_F_D07_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG TNNNNNNNNN CCTACNAGGC CGCCAAGAAG
   61 ANCCTGAAGA TGCCCCANNG CCACTTCATC GGCCACAGGC TGGTGAGGAA GACCGAGGGC
  121 AACATCACCG AGCTGGTGGA GGACGCCGTG GCCCACTGCG AGGGCAGAGG AAGTCTTCTA
  181 ACATGCGGTG ACGTGGAGGA GAATCCCGGC CCTATGTCTA GACTGGACAA GAGCAAAGTC
  241 ATAAACGGCG CTCTGGAATT ACTCAATGGA GTCGGTATCG AAGGCCTGAC GACAAGGAAA
  301 CTCGCTCAAA AGCTGGGAGT TGAGCAGCCT ACCCTGTACT GGCACGTGAA GAACAAGCGG
  361 GCCCTGCTCG ATGCCCTGCC AATCGAGATG CTGGACAGGC ATCATACCCA CTTCTGCCCC
  421 CTGGAAGGCG AGTCATGGCA AGACTTTCTG CGGAACAACG CCAAGTCATT CCGCTGTGCT
  481 CTCCTCTCAC ATCGCGACGG GGCTAAAGTG CATCTCGGCA CCCGCCCAAC AGAGAAACAG
  541 TACGAAACCC TGGAAAATCA GCTCGCGTTC CTGTGTCAGC AAGGCTTCTC CCTGGAGAAC
  601 GCACTGTACG CTCTGTCCGC CGTGGGCCAC TTTACACTGG GCTGCGTATT GGANGAACAG
  661 GAGCATCAAG TAGCAAAAGA GGAAAGAGAG ACACCTACCA CCGATTCTAT GCCCCCACTT
  721 CTGANACAAG CAATTGAGCT GTTCGACCGG CAGGGAGCCG AACCTGCCTT CCTTTTCGGC
  781 CTGNAACTAA TCATATGTGG CCNGGANAAN CAGCNAAAGT GCGAAAGCGG CGGGCCGGCC
  841 NANGCCCTTG ACGATTTTGA CTTANACATG CTCCCAGCCN ATGCCCTNNN CGACTTNGNN
901 CNTGNNATGN TGNNNGNNNN CGCNNNNGNN NANTTNNNNN NNNNN
//
Primer: CMV_Forward
Region: CMV pro, 5' LTR, HIV-1 psi
Sequence file: RDB17719_A9L9l.seq check
>D06794A2-1_A9L9_CMV-Forward_E07_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTN NNGCNNNTGG GCGGTANGCG TGTANGGTGG GNNGTCTATA
   61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG
  121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT
  181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT
  241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA
  301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG
  361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG
  421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC
  481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG
  541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA
  601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA
  661 ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG
  721 CTTTAGACAA GATAGAGGAA GAGCNAAACA AAAGTNAGAN CACCGCACAG CANGCGGCCG
  781 CNNATCTTCA GANCTGGANG ANGANATATG AGGGANNNTT GGAGAAGTGA ATTATATAAA
  841 TATAAAGTAN TAAANTTGAA NNNTTANGNN TANCNCCCAC CNAGGCAAAN ANAAGANNGN
901 NGCNNANNNN AAAAANNNAN CNNNNNNNNN N
//
Primer: BGH_rev2
Region: 3' LTR, loxP, PRE
Sequence file: RDB17719_A9L9m.seq check
>D06794A2-1_A9L9_BGH_rev2_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGGGNNGG GCNNNNNNNN GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGNTCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
  121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
  181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
  241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA ATAACTTCGT
  301 ATAATGTATG CTATACGAAG TTATGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC
  361 CAGTCCCCCC TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG
  421 TCTTAAAGGT ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG
  481 CCAGGCACAA TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC
  541 TAGGTCTCGA CGGTATCGAT GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG
  601 GCGAAGGCGA AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGGCCGCGGG AAGGAAGGTC
  661 CGCTGGATTG ANGGCCGAAN GGANGTANCA GAANGACGTC CCGCGCAGAA TCCAGGTGGC
  721 AACACAGGCG AGCAGCCATG GAAAGGACGT CAGCTTNNNC GACNACACCA CGGAATTGTC
  781 AGTGCCCNAC AGCCGANCCC CTGTCCNGCA NCGGGCAAGG CANGCGGCGA TGANTTCCGC
  841 CGNGNNNATA NGGANGGGGA AAGCGAAAGT CCCNGAAANG ANCTGANNNG NGGNGGCANN
  901 GCCCNNNNCN GNGGGGGGTT GCGNCANCAA ACNCAGNGNN NNNCACGNCN NCNNNGCNNG
961 NNNNNNGNNN NNNANNNNNN NTNAAGNNNN NGNNNNNNNN NN
//
Primer: SV40pro_ori_F
Region: SV40pro_ori, EM7 pro, Zeo
Sequence file: RDB17719_A9L9n.seq check
>D06794A2-1_A9L9_SV40pro_ori_F_G07_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCTGCCTC TGNNCTATTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
  121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC
  181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC
  241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG
  301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG
  361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG
  421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG
  481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC
  541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC
  601 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGNTGATC
  661 CTCCAGCGCG GGGNTCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT
  721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
  781 CTGCATTCTA GTTGTGGTTT GTCCNAACTC ATCAATGTAT CTTATCATGT CTGTATANNN
  841 NCNANCTCTA NCTNGAGCTT GGNGNAATCN NNGNCATANC NGTTTTCCNN NNNGAAATNG
901 NTATCCNCNC ACNANTTNCN CNCNACNNNC NANNNNGNNN CATNAANNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656.

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl