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CSIV-TRE-HPV-E6/E7-UbC-KT

Lentivirus vector HPV16 E6 and E7 with Tet-responsive promoter.

Catalog number RDB17719
Resource name CSIV-TRE-HPV-E6/E7-UbC-KT
Clone info. Lentivirus vector construct of transforming protein E6 and E7 region of human papillomavirus type 16 (HPV16) driven by Tet-responsive promoter and hKO1-2A-rtTA marker driven by UbC promoter.
The HPV16-E6/E7 genomic DNA subcloned into pENTR201 was a kind gift of Dr. Kiyono (National Cancer Center Research Institute, Japan). Expression was confirmed by the depositor with flowcytometer or fluorescence microscopy. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-TRE-RfA-UbC-KT (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Growth remarks Use host strain suitable for plasmid clones containing repeat elements and unstable inserts.
Gene/insert name Hum_Papillomavirus E6 (HPV16gp1) genomic DNA Hum_Papillomavirus E7 (HPV16gp2) genomic DNA
Originator Nakamura, Yukio |
 
Other clones in our bank Hum_Papillomavirus E6 (KEGG orthology K21807) | Hum_Papillomavirus E7 (KEGG orthology K21809) |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kurita R. et al. PLos One, 8(3): e59890, 2013)
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
Ordering Please visit Ordering instruction. 
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Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


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RDB17719 CSIV-TRE-HPV-E6/E7-UbC-KT DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles


2020.02.21

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