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CP525-4D

Expression vector of human CRMP2 constructs CP525 (residues 1-525 aa) with the phospho-mimic mutation (S522D-T509D-T514D-S518D).

Catalog number RDB17508
Resource name CP525-4D
Alternative name pET21b_hCRMP2 constructs CP525 (S522D-T509D-T514D-S518D)
Clone info. Expression vector of human collapsin response mediator protein 2 (CRMP2) constructs CP525 (residues 1-525 aa) with the phospho-mimic mutations (S522D-T509D-T514D-S518D), fused with 7×His-tag at C-terminus see Fig. 1A and 1B of Sumi, T. et al. Cell Struct. Funct. 43 (1): 15-23, 2018.
Vector backbone pET 21b (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Gene/insert name human DPYSL2 cDNA
Depositor Nitta, Ryo |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sumi, T et al. Cell Struct. Funct. 43(1): 15-23, 2018)
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17508 CP525-4D Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Sumi, T., Structural Insights into the Altering Function of CRMP2 by Phosphorylation. Cell Struct. Funct. 43 (1): 15-23 (2018). PMID 29479005.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

Original, user report and related articles

original Sumi, T., Structural Insights into the Altering Function of CRMP2 by Phosphorylation. Cell Struct. Funct. 43 (1): 15-23 (2018). PMID 29479005.

2019.11.05

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