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CP525-S522D (#RDB17507)

Expression vector of human CRMP2 constructs CP525 (residues 1-525 aa) with the phospho-mimic mutation (S522D).

Alternative name pET21b_hCRMP2 constructs CP525 (S522D)
Clone info. Expression vector of human collapsin response mediator protein 2 (CRMP2) constructs CP525 (residues 1-525 aa) with the phospho-mimic mutation (S522D), fused with 7×His-tag at C-terminus see Fig. 1A and 1B of Sumi, T. et al. Cell Struct. Funct. 43 (1): 15-23, 2018.
Vector backbone pET 21b (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Gene/insert name human DPYSL2 cDNA
Depositor|Developer Nitta, Ryo |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sumi, T et al. Cell Struct. Funct. 43(1): 15-23, 2018)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sumi, T et al. Cell Struct. Funct. 43(1): 15-23, 2018)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17507 CP525-S522D Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The CP525-S522D was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17507).

Reference section:

Sumi, T., Imasaki, T., Aoki, M., Sakai, N., Nitta, E., Shirouzu, M., Nitta, R., Structural Insights into the Altering Function of CRMP2 by Phosphorylation. Cell Struct. Funct. 43 (1): 15-23 (2018). PMID 29479005. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Sumi, T., Structural Insights into the Altering Function of CRMP2 by Phosphorylation. Cell Struct. Funct. 43 (1): 15-23 (2018). PMID 29479005. [link to RRC of NBRP]