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CP525-S522D

Expression vector of human CRMP2 constructs CP525 (residues 1-525 aa) with the phospho-mimic mutation (S522D).

Catalog number RDB17507
Resource name CP525-S522D
Alternative name pET21b_hCRMP2 constructs CP525 (S522D)
Clone info. Expression vector of human collapsin response mediator protein 2 (CRMP2) constructs CP525 (residues 1-525 aa) with the phospho-mimic mutation (S522D), fused with 7×His-tag at C-terminus see Fig. 1A and 1B of Sumi, T. et al. Cell Struct. Funct. 43 (1): 15-23, 2018.
Vector backbone pET 21b (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Gene/insert name human DPYSL2 cDNA
Depositor|Developer Nitta, Ryo |
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External Database
human DPYSL2

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sumi, T et al. Cell Struct. Funct. 43(1): 15-23, 2018)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sumi, T et al. Cell Struct. Funct. 43(1): 15-23, 2018)。
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          RDB17507 CP525-S522D Under QC test. Please contact us. DNA solution

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          How to cite this biological resource

          Materials & Methods section:

          The CP525-S522D was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17507).

          Reference section:

          Sumi, T., Imasaki, T., Aoki, M., Sakai, N., Nitta, E., Shirouzu, M., Nitta, R., Structural Insights into the Altering Function of CRMP2 by Phosphorylation. Cell Struct. Funct. 43 (1): 15-23 (2018). PMID 29479005. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

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          References

          Original, user report and related articles

          original Sumi, T., Structural Insights into the Altering Function of CRMP2 by Phosphorylation. Cell Struct. Funct. 43 (1): 15-23 (2018). PMID 29479005. [link to RRC of NBRP]

          2023.07.31

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