Plasmid vector for blunt-end cloning of PCR-products.
Catalog number | RDB17481 |
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Resource name | pCRZero |
Clone info. | Plasmid vector for blunt-end cloning of PCR-products using ZERO-background cloning system. Map and cloning site region, see Fig. 1 of Motohashi, K. Sci. Rep. 9 (1): 6417, 2019. |
Vector backbone | pUC18 (plasmid) |
Size of vector backbone | 2.7 kb |
Selectable markers | Ampicillin |
Growth remarks | Use XL10-Gold to amplify this clone as mentioned in Motohashi, K. Sci. Rep. 9 (1): 6417, 2019. LB+Ampicillin, add final concentration of 1% glucose, 37oC. |
Gene/insert name | E. coli ccdB genomic DNA |
Depositor|Developer | Motohashi, Ken | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Motohashi, K. Sci. Rep. 9(1): 6417, 2019) and an acknowledgment to the DEPOSITOR are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA) [Word ![]() |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB17481 | pCRZero | DNA solution |
Electronic file
Electronic file | Map RDB17481z.png
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Original reference
original | Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513. |
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Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Empty Backbone (English text) |
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Featured content | Empty Backbone (Japanese text) |
Featured content | A series of high-efficiency PCR-cloning vectors and their useful application [link] (Japanese text) |
Featured content | A series of high-efficiency PCR-cloning vectors and their useful application [link] (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB17481_A9Hqp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pUC-CAPbs_up_F Region: CAP site, lac pro, lac op, lacZalpha-ccdB(EcoRV) 5' Sequence file: RDB17481_A9Hqa.seq ![]() |
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>D06545A3_A9Hq_1_pUC-CAPbs_up_F_E12_15_ABI24.ab1 1 NNNNNNNNNN NNNGNNNNNN NNNCGCNNNG CAATNAATGT GAGTTAGCTC ACTCATTAGG 61 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT 121 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTATTT AGGTGACACT 181 ATAGAATACT CAAGCTATGC ATCAAGCTTG GTACCGAGCT CGGATCCACT AGTAACGGCC 241 GCCAGTGTGC TGGAATTCTG CAGATATCCA TCACACTGGC GGCCGCTCGA GCATGCATCT 301 AGAGGGCCCA ATTCGCCCTA TTCGAAGTCG TATTACAATT CACTGGCCGT CGTTTTACAA 361 CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT 421 TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC 481 AGCCTATACG TACGGCAGTT TAAGGTTTAC ACCTATAAAA GAGAGAGCCG TTATCGTCTG 541 TTTGTGGATG TACAGAGTGA TATTATTGAC ACGCCGGGGC GACGGATGGT GATCCCCCTG 601 GCCAGTGCAC GTCTGCTGTC AGATAAAGTC TCCCGTGAAC TTTACCCGGT GGTGCATATC 661 GGGGATGAAA GCTGGCGCAT GATGACCACC GATATGGCCA GTGTGCCGGT CTCCGTTATC 721 GGGGAAGAAG TGGCTGATCT CAGCCACCGC GAAAATGACA TCAAAAACGC CATTANCCTG 781 ATGTTCTGGG GAATATAACT TGNCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA 841 NCCTGGCGTT ACNCAACTTA NTCGCCTTGC AGCACATCCC CNTTTCGCCA GCTGGNGTAN 901 TANCGAANAN GNCCGCACNN ATCGCCCTTC CCNACANTTG CGCANCCNNA ATGGCGAANN 961 GNNGCCNNAN NNNGNATTTN NNNCTTNNNN NNNNNN // |
Primer: tetpro-B_R Region: lacZalpha-ccdB(EcoRV) 3' Sequence file: RDB17481_A9Hqb.seq ![]() |
>D06545A3_A9Hq_1_tetpro-B_R_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTGCAGCTC CCGGNNNNGG TCACAGCTTG 61 TCTGTAAGCG GATGCCGGGN NCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG 121 GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT 181 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC 241 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA 301 GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA 361 GTCACGACGT TGTAAAACGA CGGCCAGTGC CAAGTTATAT TCCCCAGAAC ATCAGGTTAA 421 TGGCGTTTTT GATGTCATTT TCGCGGTGGC TGAGATCAGC CACTTCTTCC CCGATAACGG 481 AGACCGGCAC ACTGGCCATA TCGGTGGTCA TCATGCGCCA GCTTTCATCC CCGATATGCA 541 CCACCGGGTA AAGTTCACGG GAGACTTTAT CTGACAGCAG ACGTGCACTG GCCAGGGGGA 601 TCACCATCCG TCGCCCCGGC GTGTCAATAA TATCACTCTG TACATCCACA AACAGACGAT 661 AACGGCTCTC TCTTTTATAG GTGTAAACCT TAAACTGCCG TACGTATAGG CTGCGCAACT 721 GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCNGCTGGCG AAAGGGGGAT 781 GTGCTGCAAG GCGATTAAGT TGGGTAACGC CNGGGTTTTC CCAGTCACGA CGTTGTAAAA 841 CGACNGCCAG TGANNTGTAA TACNACTTCN AATANGGCGA ANTGNGCCCT CTAGATGCAT 901 GCTCGAGCGG NCNNCNGNGT GANGGATATC TGCANAATNN CNNCANACTN NNNGNNNNTA 961 CTAGNGGNNN CNNANCTCNG NNNCNNAGCT TGANGNNNAN CTTGNNNANN NNANNNNGNN 1021 NCCNNAATNN CNNNNNNNNN N // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513. |
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2020.10.02
GNP_filter3_RDBDEP_html_200316.pl