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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCRZero

Plasmid vector for blunt-end cloning of PCR-products.

Catalog number RDB17481
Resource name pCRZero
Clone info. Plasmid vector for blunt-end cloning of PCR-products using ZERO-background cloning system. Map and cloning site region, see Fig. 1 of Motohashi, K. Sci. Rep. 9 (1): 6417, 2019.
Vector backbone pUC18 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Growth remarks Use XL10-Gold to amplify this clone as mentioned in Motohashi, K. Sci. Rep. 9 (1): 6417, 2019. LB+Ampicillin, add final concentration of 1% glucose, 37oC.
Gene/insert name E. coli ccdB genomic DNA
Depositor|Developer Motohashi, Ken |
Other clones in our bank
External Database
E. coli ccdB
            Reference sequence
              
             
            Remarks, protocol and/or map (png) RDB17481z.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Motohashi, K. Sci. Rep. 9(1): 6417, 2019) and an acknowledgment to the DEPOSITOR are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Motohashi, K. Sci. Rep. 9(1): 6417, 2019) を引用し、謝辞の表明を必要とする。学術機関の学術研究に限る。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB17481 pCRZero DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pCRZero was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17481).

            Reference section:

            Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513.

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            Featured content A series of high-efficiency PCR-cloning vectors and their useful application [link] (English text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)
            Featured content A series of high-efficiency PCR-cloning vectors and their useful application [link] (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17481_A9Hqp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-CAPbs_up_F (Pr0536)
            Region: CAP site, lac pro, lac op, lacZalpha-ccdB(EcoRV) 5'
            Sequence file: RDB17481_A9Hqa.seq check
            >D06545A3_A9Hq_1_pUC-CAPbs_up_F_E12_15_ABI24.ab1
                1 NNNNNNNNNN NNNGNNNNNN NNNCGCNNNG CAATNAATGT GAGTTAGCTC ACTCATTAGG
               61 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT
              121 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTATTT AGGTGACACT
              181 ATAGAATACT CAAGCTATGC ATCAAGCTTG GTACCGAGCT CGGATCCACT AGTAACGGCC
              241 GCCAGTGTGC TGGAATTCTG CAGATATCCA TCACACTGGC GGCCGCTCGA GCATGCATCT
              301 AGAGGGCCCA ATTCGCCCTA TTCGAAGTCG TATTACAATT CACTGGCCGT CGTTTTACAA
              361 CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT
              421 TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC
              481 AGCCTATACG TACGGCAGTT TAAGGTTTAC ACCTATAAAA GAGAGAGCCG TTATCGTCTG
              541 TTTGTGGATG TACAGAGTGA TATTATTGAC ACGCCGGGGC GACGGATGGT GATCCCCCTG
              601 GCCAGTGCAC GTCTGCTGTC AGATAAAGTC TCCCGTGAAC TTTACCCGGT GGTGCATATC
              661 GGGGATGAAA GCTGGCGCAT GATGACCACC GATATGGCCA GTGTGCCGGT CTCCGTTATC
              721 GGGGAAGAAG TGGCTGATCT CAGCCACCGC GAAAATGACA TCAAAAACGC CATTANCCTG
              781 ATGTTCTGGG GAATATAACT TGNCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA
              841 NCCTGGCGTT ACNCAACTTA NTCGCCTTGC AGCACATCCC CNTTTCGCCA GCTGGNGTAN
              901 TANCGAANAN GNCCGCACNN ATCGCCCTTC CCNACANTTG CGCANCCNNA ATGGCGAANN
            961 GNNGCCNNAN NNNGNATTTN NNNCTTNNNN NNNNNN
            //
            Primer: tetpro-B_R (Pr0732)
            Region: lacZalpha-ccdB(EcoRV) 3'
            Sequence file: RDB17481_A9Hqb.seq check
            >D06545A3_A9Hq_1_tetpro-B_R_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTGCAGCTC CCGGNNNNGG TCACAGCTTG
               61 TCTGTAAGCG GATGCCGGGN NCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG
              121 GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT
              181 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC
              241 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA
              301 GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA
              361 GTCACGACGT TGTAAAACGA CGGCCAGTGC CAAGTTATAT TCCCCAGAAC ATCAGGTTAA
              421 TGGCGTTTTT GATGTCATTT TCGCGGTGGC TGAGATCAGC CACTTCTTCC CCGATAACGG
              481 AGACCGGCAC ACTGGCCATA TCGGTGGTCA TCATGCGCCA GCTTTCATCC CCGATATGCA
              541 CCACCGGGTA AAGTTCACGG GAGACTTTAT CTGACAGCAG ACGTGCACTG GCCAGGGGGA
              601 TCACCATCCG TCGCCCCGGC GTGTCAATAA TATCACTCTG TACATCCACA AACAGACGAT
              661 AACGGCTCTC TCTTTTATAG GTGTAAACCT TAAACTGCCG TACGTATAGG CTGCGCAACT
              721 GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCNGCTGGCG AAAGGGGGAT
              781 GTGCTGCAAG GCGATTAAGT TGGGTAACGC CNGGGTTTTC CCAGTCACGA CGTTGTAAAA
              841 CGACNGCCAG TGANNTGTAA TACNACTTCN AATANGGCGA ANTGNGCCCT CTAGATGCAT
              901 GCTCGAGCGG NCNNCNGNGT GANGGATATC TGCANAATNN CNNCANACTN NNNGNNNNTA
              961 CTAGNGGNNN CNNANCTCNG NNNCNNAGCT TGANGNNNAN CTTGNNNANN NNANNNNGNN
            1021 NCCNNAATNN CNNNNNNNNN N
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513.

            2023.05.22

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