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pCRZero (#RDB17481)

Plasmid vector for blunt-end cloning of PCR-products.

Clone info. Plasmid vector for blunt-end cloning of PCR-products using ZERO-background cloning system. Map and cloning site region, see Fig. 1 of Motohashi, K. Sci. Rep. 9 (1): 6417, 2019.
Vector backbone pUC18 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Growth conditions LB+Ampicillin, add final concentration of 1% glucose, 37oC
Growth remarks Use XL10-Gold to amplify this clone as mentioned in Motohashi, K. Sci. Rep. 9 (1): 6417, 2019. LB+Ampicillin, add final concentration of 1% glucose, 37oC.
Gene/insert name E. coli ccdB genomic DNA
Depositor|Developer Motohashi, Ken |
 
Sequence (full) RDB17481hts01.seq checkAssembled from experimentally sequenced data.
Additional map
Publication Motohashi, K., Sci. Rep. 9 (1): 6417 (2019). [link to RRC of NBRP]
Test sheet RDB17481_B0H4p1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Motohashi, K. Sci. Rep. 9(1): 6417, 2019) and an acknowledgment to the DEPOSITOR are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Motohashi, K. Sci. Rep. 9(1): 6417, 2019) を引用し、謝辞の表明を必要とする。学術機関の学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17481 pCRZero DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCRZero was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17481).

Reference section:

Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513. [link to RRC of NBRP]