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pCRZero

Plasmid vector for blunt-end cloning of PCR-products.

Catalog number RDB17481
Resource name pCRZero
Clone info. Plasmid vector for blunt-end cloning of PCR-products using ZERO-background cloning system. Map and cloning site region, see Fig. 1 of Motohashi, K. Sci. Rep. 9 (1): 6417, 2019.
Vector backbone pUC18 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Growth remarks Use XL10-Gold to amplify this clone as mentioned in Motohashi, K. Sci. Rep. 9 (1): 6417, 2019. LB+Ampicillin, add final concentration of 1% glucose, 37oC.
Gene/insert name E. coli ccdB genomic DNA
Depositor Motohashi, Ken |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Motohashi, K. Sci. Rep. 9(1): 6417, 2019) and an acknowledgment to the DEPOSITOR are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
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RDB17481 pCRZero DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB17481z.png

Original reference

original Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB17481_A9Hqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: CAP site, lac pro, lac op, lacZalpha-ccdB(EcoRV) 5' | Primer: pUC-CAPbs_up_F | Sequence: RDB17481_A9Hqa.seq
>D06545A3_A9Hq_1_pUC-CAPbs_up_F_E12_15_ABI24.ab1
    1 NNNNNNNNNN NNNGNNNNNN NNNCGCNNNG CAATNAATGT GAGTTAGCTC ACTCATTAGG
   61 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT
  121 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTATTT AGGTGACACT
  181 ATAGAATACT CAAGCTATGC ATCAAGCTTG GTACCGAGCT CGGATCCACT AGTAACGGCC
  241 GCCAGTGTGC TGGAATTCTG CAGATATCCA TCACACTGGC GGCCGCTCGA GCATGCATCT
  301 AGAGGGCCCA ATTCGCCCTA TTCGAAGTCG TATTACAATT CACTGGCCGT CGTTTTACAA
  361 CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT
  421 TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC
  481 AGCCTATACG TACGGCAGTT TAAGGTTTAC ACCTATAAAA GAGAGAGCCG TTATCGTCTG
  541 TTTGTGGATG TACAGAGTGA TATTATTGAC ACGCCGGGGC GACGGATGGT GATCCCCCTG
  601 GCCAGTGCAC GTCTGCTGTC AGATAAAGTC TCCCGTGAAC TTTACCCGGT GGTGCATATC
  661 GGGGATGAAA GCTGGCGCAT GATGACCACC GATATGGCCA GTGTGCCGGT CTCCGTTATC
  721 GGGGAAGAAG TGGCTGATCT CAGCCACCGC GAAAATGACA TCAAAAACGC CATTANCCTG
  781 ATGTTCTGGG GAATATAACT TGNCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA
  841 NCCTGGCGTT ACNCAACTTA NTCGCCTTGC AGCACATCCC CNTTTCGCCA GCTGGNGTAN
  901 TANCGAANAN GNCCGCACNN ATCGCCCTTC CCNACANTTG CGCANCCNNA ATGGCGAANN
  961 GNNGCCNNAN NNNGNATTTN NNNCTTNNNN NNNNNNNNNG NTANTNNNNN NNNNNNNNNN
 1021 NGNNNNNNNN NNNNNANNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNN
//
Region: lacZalpha-ccdB(EcoRV) 3' | Primer: tetpro-B_R | Sequence: RDB17481_A9Hqb.seq
>D06545A3_A9Hq_1_tetpro-B_R_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTGCAGCTC CCGGNNNNGG TCACAGCTTG
   61 TCTGTAAGCG GATGCCGGGN NCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG
  121 GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT
  181 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC
  241 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA
  301 GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA
  361 GTCACGACGT TGTAAAACGA CGGCCAGTGC CAAGTTATAT TCCCCAGAAC ATCAGGTTAA
  421 TGGCGTTTTT GATGTCATTT TCGCGGTGGC TGAGATCAGC CACTTCTTCC CCGATAACGG
  481 AGACCGGCAC ACTGGCCATA TCGGTGGTCA TCATGCGCCA GCTTTCATCC CCGATATGCA
  541 CCACCGGGTA AAGTTCACGG GAGACTTTAT CTGACAGCAG ACGTGCACTG GCCAGGGGGA
  601 TCACCATCCG TCGCCCCGGC GTGTCAATAA TATCACTCTG TACATCCACA AACAGACGAT
  661 AACGGCTCTC TCTTTTATAG GTGTAAACCT TAAACTGCCG TACGTATAGG CTGCGCAACT
  721 GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCNGCTGGCG AAAGGGGGAT
  781 GTGCTGCAAG GCGATTAAGT TGGGTAACGC CNGGGTTTTC CCAGTCACGA CGTTGTAAAA
  841 CGACNGCCAG TGANNTGTAA TACNACTTCN AATANGGCGA ANTGNGCCCT CTAGATGCAT
  901 GCTCGAGCGG NCNNCNGNGT GANGGATATC TGCANAATNN CNNCANACTN NNNGNNNNTA
  961 CTAGNGGNNN CNNANCTCNG NNNCNNAGCT TGANGNNNAN CTTGNNNANN NNANNNNGNN
 1021 NCCNNAATNN CNNNNNNNNN NNNNNNNNNN NNNNNNTNNN NNNNNNNNAN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513.

2019.11.06

GNP_filter3_RDBDEP_html_191106.pl