Bifunctional plasmid vector for TA-cloning and blunt-end cloning of PCR-products.
Catalog number | RDB17480 |
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Resource name | pCRZeroT |
Clone info. | Bifunctional plasmid vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-background cloning system. Map and cloning site region, see Fig. 1 of Motohashi, K. Sci. Rep. 9 (1): 6417, 2019. |
Vector backbone | pUC18 (plasmid) |
Size of vector backbone | 2.7 kb |
Selectable markers | Ampicillin |
Growth remarks | Use XL10-Gold to amplify this clone as mentioned in Motohashi, K. Sci. Rep. 9 (1): 6417, 2019. LB+Ampicillin, add final concentration of 1% glucose, 37oC. |
Gene/insert name | E. coli ccdB genomic DNA |
Depositor|Developer | Motohashi, Ken | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Motohashi, K. Sci. Rep. 9(1): 6417, 2019) and an acknowledgment to the DEPOSITOR are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA) [Word ![]() |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB17480 | pCRZeroT | DNA solution |
Electronic file
Electronic file | Map RDB17480z.png
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Original reference
original | Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513. |
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Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Empty Backbone (English text) |
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Featured content | Empty Backbone (Japanese text) |
Featured content | A series of high-efficiency PCR-cloning vectors and their useful application [link] (Japanese text) |
Featured content | A series of high-efficiency PCR-cloning vectors and their useful application [link] (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB17480_A9Hqp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pUC-CAPbs_up_F Region: CAP site, lac pro, lac op, XcmI, lacZ alpha N-ccdB, lacZ alpha C,XcmI Sequence file: RDB17480_A9Hqa.seq ![]() |
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>D06545A2_A9Hq_2_pUC-CAPbs_up_F_C12_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNCGNACGC AATTAATGTG AGTTAGCTCN CTCATTAGGC 61 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA 121 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGCCAATA CTTGTATGGC 181 AGTTTAAGGT TTACACCTAT AAAAGAGAGA GCCGTTATCG TCTGTTTGTG GATGTACAGA 241 GTGATATTAT TGACACGCCC GGGCGACGGA TGGTGATCCC CCTGGCCAGT GCACGTCTGC 301 TGTCAGATAA AGTCTCCCGT GAACTTTACC CGGTGGTGCA TATCGGGGAT GAAAGCTGGC 361 GCATGATGAC CACCGATATG GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG 421 ATCTCAGCCA CCGCGAAAAT GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT 481 AACCATACAA GTATTGGAAG CTTGGCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA 541 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT 601 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA 661 TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG 721 TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA 781 ACACCCGCTG ANGCGCCCTG ACGGGCTNGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT 841 GTGACCGTCT CCGGGANCTG CATGTGTCAG ANGTTTTCAC CGTCATCACC NAAACGCGCG 901 ANANNAAAGG GCCTCNNGAT ACGCCNATTT TTNATAGGTT NATGNCATGA TAATANTGNN 961 TTCTTANNNN NCNNNNNNNN NTTTTTCGGG NNANNNNNNN NNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513. |
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2020.10.02
GNP_filter3_RDBDEP_html_200316.pl