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pCRZeroT

Bifunctional plasmid vector for TA-cloning and blunt-end cloning of PCR-products.

Catalog number RDB17480
Resource name pCRZeroT
Clone info. Bifunctional plasmid vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-background cloning system. Map and cloning site region, see Fig. 1 of Motohashi, K. Sci. Rep. 9 (1): 6417, 2019.
Vector backbone pUC18 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Growth remarks Use XL10-Gold to amplify this clone as mentioned in Motohashi, K. Sci. Rep. 9 (1): 6417, 2019. LB+Ampicillin, add final concentration of 1% glucose, 37oC.
Gene/insert name E. coli ccdB genomic DNA
Depositor Motohashi, Ken |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Motohashi, K. Sci. Rep. 9(1): 6417, 2019) and an acknowledgment to the DEPOSITOR are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
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RDB17480 pCRZeroT DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB17480z.png

Original reference

original Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB17480_A9Hqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: CAP site, lac pro, lac op, XcmI, lacZ alpha N-ccdB, lacZ alpha C,XcmI | Primer: pUC-CAPbs_up_F | Sequence: RDB17480_A9Hqa.seq
>D06545A2_A9Hq_2_pUC-CAPbs_up_F_C12_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCGNACGC AATTAATGTG AGTTAGCTCN CTCATTAGGC
   61 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA
  121 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGCCAATA CTTGTATGGC
  181 AGTTTAAGGT TTACACCTAT AAAAGAGAGA GCCGTTATCG TCTGTTTGTG GATGTACAGA
  241 GTGATATTAT TGACACGCCC GGGCGACGGA TGGTGATCCC CCTGGCCAGT GCACGTCTGC
  301 TGTCAGATAA AGTCTCCCGT GAACTTTACC CGGTGGTGCA TATCGGGGAT GAAAGCTGGC
  361 GCATGATGAC CACCGATATG GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG
  421 ATCTCAGCCA CCGCGAAAAT GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT
  481 AACCATACAA GTATTGGAAG CTTGGCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA
  541 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT
  601 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA
  661 TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG
  721 TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA
  781 ACACCCGCTG ANGCGCCCTG ACGGGCTNGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT
  841 GTGACCGTCT CCGGGANCTG CATGTGTCAG ANGTTTTCAC CGTCATCACC NAAACGCGCG
  901 ANANNAAAGG GCCTCNNGAT ACGCCNATTT TTNATAGGTT NATGNCATGA TAATANTGNN
  961 TTCTTANNNN NCNNNNNNNN NTTTTTCGGG NNANNNNNNN NNNNANNNCC NNNNTNNNTN
 1021 NNTTTTNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513.

2019.11.06

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