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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCRZeroT

Bifunctional plasmid vector for TA-cloning and blunt-end cloning of PCR-products.

Catalog number RDB17480
Resource name pCRZeroT
Clone info. Bifunctional plasmid vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-background cloning system. Map and cloning site region, see Fig. 1 of Motohashi, K. Sci. Rep. 9 (1): 6417, 2019.
Vector backbone pUC18 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Growth remarks Use XL10-Gold to amplify this clone as mentioned in Motohashi, K. Sci. Rep. 9 (1): 6417, 2019. LB+Ampicillin, add final concentration of 1% glucose, 37oC.
Gene/insert name E. coli ccdB genomic DNA
Depositor|Developer Motohashi, Ken |
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External Database
E. coli ccdB
            Reference sequence
              
             
            Remarks, protocol and/or map (png) RDB17480z.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Motohashi, K. Sci. Rep. 9(1): 6417, 2019) and an acknowledgment to the DEPOSITOR are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Motohashi, K. Sci. Rep. 9(1): 6417, 2019) を引用し、謝辞の表明を必要とする。学術機関の学術研究に限る。
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            RDB17480 pCRZeroT DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pCRZeroT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17480).

            Reference section:

            Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17480_A9Hqp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-CAPbs_up_F (Pr0536)
            Region: CAP site, lac pro, lac op, XcmI, lacZ alpha N-ccdB, lacZ alpha C,XcmI
            Sequence file: RDB17480_A9Hqa.seq check
            >D06545A2_A9Hq_2_pUC-CAPbs_up_F_C12_09_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNCGNACGC AATTAATGTG AGTTAGCTCN CTCATTAGGC
               61 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA
              121 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGCCAATA CTTGTATGGC
              181 AGTTTAAGGT TTACACCTAT AAAAGAGAGA GCCGTTATCG TCTGTTTGTG GATGTACAGA
              241 GTGATATTAT TGACACGCCC GGGCGACGGA TGGTGATCCC CCTGGCCAGT GCACGTCTGC
              301 TGTCAGATAA AGTCTCCCGT GAACTTTACC CGGTGGTGCA TATCGGGGAT GAAAGCTGGC
              361 GCATGATGAC CACCGATATG GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG
              421 ATCTCAGCCA CCGCGAAAAT GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT
              481 AACCATACAA GTATTGGAAG CTTGGCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA
              541 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT
              601 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA
              661 TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG
              721 TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA
              781 ACACCCGCTG ANGCGCCCTG ACGGGCTNGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT
              841 GTGACCGTCT CCGGGANCTG CATGTGTCAG ANGTTTTCAC CGTCATCACC NAAACGCGCG
              901 ANANNAAAGG GCCTCNNGAT ACGCCNATTT TTNATAGGTT NATGNCATGA TAATANTGNN
            961 TTCTTANNNN NCNNNNNNNN NTTTTTCGGG NNANNNNNNN NNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513. [link to RRC of NBRP]

            2024.03.26

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