Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCRT

Plasmid vector for TA-cloning of PCR-products.

Catalog number RDB17479
Resource name pCRT
Clone info. Plasmid vector for TA-cloning of PCR-products. Map and cloning site region, see Fig. 1 of Motohashi, K. Sci. Rep. 9 (1): 6417, 2019.
Vector backbone pUC18 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Depositor|Developer Motohashi, Ken |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Motohashi, K. Sci. Rep. 9(1): 6417, 2019) and an acknowledgment to the DEPOSITOR are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17479 pCRT DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Map RDB17479z.png

Original reference

original Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)
Featured content A series of high-efficiency PCR-cloning vectors and their useful application [link] (Japanese text)
Featured content A series of high-efficiency PCR-cloning vectors and their useful application [link] (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17479_A9Hqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F
Region: CAP site, lac pro, lac op, lacZ alpha(Xcml-NheI-XcmI)
Sequence file: RDB17479_A9Hqa.seq check
>D06545A1_A9Hq_3_pUC-CAPbs_up_F_A12_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCNNNNNC AATTAATGTG AGTTAGCTCA CTCATTAGGC
   61 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA
  121 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGAGCTCG GTACCCCAAT
  181 ACTTGTATGG AGACGCTAGC GTCTCCATAC AAGTATTGGG GGATCCTCTA GAGTCGACCT
  241 GCAGGCATGC AAGCTTGGCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG
  301 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG
  361 AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGCGCC
  421 TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC
  481 TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG
  541 CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG
  601 TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA
  661 AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA
  721 CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA
  781 TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG NTAAATGCTT CAATAATATT
  841 GAAAAAGGAA GAGTATGANT ATTCNACATT TCCGTGTCGC CCNTATTCCC NTTTTTNGNG
  901 GCATTTNGCC TTNCNGNTTT TTGCTCACCC AGAANNGCTN GNGAANNTAA AAGANGCTGA
961 AGANCAGNTN NGNNGCNNNN NNGGNNNNNN TCGNANNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513.

2020.10.02

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