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pBAD33Ts-Tu-beta-3

Bacterial expression vector of E. coli EF-Tu and EF-Ts, and beta-subunit of Q-beta replicase.

Catalog number RDB17459
Resource name pBAD33Ts-Tu-beta-3
Clone info. Bacterial expression vector of E. coli EF-Tu and EF-Ts, and beta-subunit of Q-beta replicase with His-tag. Schematic of this clone, see Fig.2A and B of Kita, H. et al., J. Biosci. Bioeng., 101 (5) :421-426, 2006.
The plasmids pBAD33 was kindly provided by Dr. S. Yasuda of the National Institute of Genetics, Japan. The plasmid ZpQbeta32 containing cDNA of the Qbeta phage genome was kindly provided by Dr. Y. Inokuchi. Expression was confirmed by the depositor with SDS-PAGE and CBB staining.
Vector backbone pBAD33 (plasmid)
Selectable markers Chloramphenicol
Gene/insert name E. coli EF-Tu, EF-Ts genomic
Depositor Ichihashi, Norikazu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biosci. Bioeng. 101(5), 421-426, 2006)
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RDB17459 pBAD33Ts-Tu-beta-3 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kita, H., Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit. J. Biosci. Bioeng. 101 (5): 421-426 (2006). PMID 16781472.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17459_A9Fop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: araBADpro_F2
Region: araBADpro, insert 5' (tsf)
Sequence file: RDB17459_A9Foa.seq check
>D06471A6_A9Fo_4_araBADpro_F2_G06_21_ABI24.ab1
    1 NNNNNNNNNN NNCNGNNNNN NNNCNNCNTT GNNTATTTGC ACGGCGTCNC ACTTTGCTAT
   61 GCCATAGCAT TTTTATCCNT AAGATTAGCG GATCCTACCT GACGCTTTTT ATCGCAACTC
  121 TCTACTGTTT CTCCATACCC GTTTTTTTGG GCTAGCAGGA GGAATTCACC ATGGCTGAAA
  181 TTACCGCATC CCTGGTAAAA GAGCTGCGTG AGCGTACTGG CGCAGGCATG ATGGATTGCA
  241 AAAAAGCACT GACTGAAGCT AACGGCGACA TCGAGCTGGC AATCGAAAAC ATGCGTAAGT
  301 CCGGTGCTAT TAAAGCAGCG AAAAAAGCAG GCAACGTTGC TGCTGACGGC GTGATCAAAA
  361 CCAAAATCGA CGGCAACTAC GGCATCATTC TGGAAGTTAA CTGCCAGACT GACTTCGTTG
  421 CAAAAGACGC TGGTTTCCAG GCGTTCGCAG ACAAAGTTCT GGACGCAGCT GTTGCTGGCA
  481 AAATCACTGA CGTTGAAGTT CTGAAAGCAC AGTTCGAAGA AGAACGTGTT GCGCTGGTAG
  541 CGAAAATTGG TGAAAACATC AACATTCGCC GCGTTGCTGC GCTGGAAGGC GACGTTCTGG
  601 GTTCTTATCA GCACGGTGCG CGTATCGGCG TTCTGGTTGC TGCTAAAGGC GCTGACGAAG
  661 AGCTGGTTAA ACACATCGCT ATGCACGTTG CTGCAAGCAA GCCAGAATTC ATCAAACCGG
  721 AAGACGTATC CGCTGAAGTG GTAGAAAAAG AATACCAGGT ACAGCTGGAT ATCGCGATGC
  781 AGTCTGGTAA GCCGAAAGAA ATCGCAGANA AAATGGNTGA AGGNCGCATG AAGANATTCA
  841 CCGGCGAANT TTCTCTGACC GGTCAGCCGN TCGTTATGGA ANCNAGCAAA ACTGTTGGTC
  901 AGCTGCTGAA AGANCATAAC GCTNAAGTGN NNGNNTTCNT CCNCTTCNAA NTGGGNGNAN
  961 NNNATCNANA ANNTTGANNN TGNNTTTGCN NCANANNNNG CNNCNNNNNN CNNNNNNNNN
 1021 NNNNNNNNNN NANNNAANNN TNNNNNNNNN NNANNNNNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NN
//
Primer: rrnBT1ter_F2
Region: 6xHis, insert 3' (Qbeta)
Sequence file: RDB17459_A9Fob.seq check
>D06471A6_A9Fo_4_rrnBT1ter_F2_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NCNNNNNNTT TGATGCCTGG CANGTTCCCT
   61 ACTCTCGCAT GGGGAGACCC CACNNNNNNA TCGGCGCTAC GGCGTTTCAC TTCTGAGTTC
  121 GGCATGGGGT CAGGTGGGAC CACCGCGCTA CTGCCGCCAG GCAAATTCTG TTTTATCAGA
  181 CCGCTTCTGC GTTCTGATTT AATCTGTATC AGGCTGAAAA TCTTCTCTCA TCCGCCAAAA
  241 CAGCCAAGCT TCGTAAATTC TATACAAAAT TAGTGGTGGT GGTGGTGGTG CGCCTCGTGT
  301 AGAGACGCAA CCTTCGTGCC CTGGAAGACC CCGTAGGGTG CCAGAACACG GCTACTGCAC
  361 GCGATATACT GTATATCGAA TTTGCCGGTA GACCTGCTTA TCTTCGTAGG ATTACTCCTA
  421 CAGATAAGCT GATCGATGGC AAATAGATCC GCAGAATCGC AACCCGATGG ACCCCTAAGA
  481 GGCAACCCAT CGTTACTTTC CGAGAGACAA CGCGAGAAGA GGTCGTAGAG ATACGACCCC
  541 AACTCAGCGC GCTCTCGGTC CCTTGTATGG TCCGTAATCA CCGGTACGTA CCGGATCCAC
  601 CCGCGGTTTT TCGCGAAAGG ATTGATTAGG ACCGATCCGA CGAGGNNNNN NTCACCGTAA
  661 CCATCAGGTA TAGTATTACG TNGNNNNTGT TTAGGCAGCA ACTTACGATA CTTGAGGTAC
  721 ACAGAATGGG CCCTAGGNNC CCATACGCCG TCAATTGTGG CCCACCGATA TAGGTTATTC
  781 NAAACCAGTA TTAAATCGGC AGGACTCACT ATACGGTGAN GTATGTANAA NGGAGTAACN
  841 TCTACNCCNN AATAGTANTG NTTGCCGCAC GACTCTCNNN NCGGCCCNTN NGGAAAAAGN
  901 CTTTTTNAGT ANNNGNNCGN NAAANNCAAC NTNCTNNAAA AACTNNCCNG NANGGGNNNG
  961 GACTGCNCAG GANNGGTAAA ANTANNATCG TNNNNNNNTA AANNNGNGAN NNCNNNNNNA
 1021 GTCTNAANNN NCAGTNNNNN NACAAACNGN AACGANGCNN ANNANNANNN NNAAANNANN
 1081 NNNNNNNNNN NNNNNNNNAN NNNNNNNNNN NNNNTNNNNN NNNNNNNNNA NNNNNNNNNN
 1141 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1201 NNNNNNNNNN N
//
Primer: RDB17459_F3
Region: insert (tsf, tuf)
Sequence file: RDB17459_A9Foc.seq check
>D06471A6_A9Fo_4_RDB17459_F3_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTG ANNNCGCATG NNAAATTCNC CGGCGAAGTT TCTCTGACCG
   61 GTCAGCCGTT CGTTATGGAA CCAAGCAAAA CTGTTGGTCA GCTGCTGAAA GAGCATAACG
  121 CTGAAGTGAC TGGCTTCATC CGCTTCGAAG TGGGTGAAGG CATCGAGAAA GTTGAGACTG
  181 ACTTTGCAGC AGAAGTTGCT GCGATGTCCA AGCAGTCTCA TATGTCTAAA GAAAAGTTTG
  241 AACGTACAAA ACCGCACGTT AACGTCGGTA CTATCGGCCA CGTTGACCAT GGTAAAACAA
  301 CGCTGACCGC TGCAATCACT ACCGTACTGG CTAAAACCTA CGGCGGTGCT GCTCGCGCAT
  361 TCGACCAGAT CGATAACGCG CCGGAAGAAA AAGCTCGTGG TATCACCATC AACACTTCTC
  421 ACGTTGAATA CGACACCCCG ACCCGTCACT ACGCACACGT AGACTGCCCG GGGCACGCCG
  481 ACTATGTTAA AAACATGATC ACCGGTGCTG CGCAGATGGA CGGCGCGATC CTGGTAGTTG
  541 CTGCGACTGA CGGCCCGATG CCGCAGACTC GTGAGCACAT CCTGCTGGGT CGTCAGGTAG
  601 GCGTTCCGTA CATCATCGTG TTCCTGAACA AATGCGACAT GGTTGATGAC NAAGAGCTGC
  661 TGGAACTGGT TGAAATGGAA GTTCGTGAAC TTCTGTCTCA NTACGACTTC NCGGGCGACN
  721 ACACTCCGAT CGTTCGNGNN NNNGCNCTGA AAGCGCTGGA NNGCGACGCN NAGTGGGAAN
  781 NNNNNNNNNN GNAACTGGCT GGGCTNCNNN NATNCTTANN NNTNCNNGAN NNNNANCGTG
  841 NNNNNNNNNN NNNNNTTNNT NNNNNNNNNN NNNNANNNNN NNNCNNNNNN NNNNNNNNNN
901 NNNNNNNNNN NNNN
//
Primer: RDB17459_F2
Region: insert (tuf, Qbeta)
Sequence file: RDB17459_A9Fod.seq check
>D06471A6_A9Fo_4_RDB17459_F2_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNNC TGTNCTGGCG TTGANTGTTC CGCAAACTGC
   61 TGGACGAAGG CCGTGCTGGT GAGAACGTAG GTGTTCTGCT GCGTGGTATC AAACGTGAAG
  121 AAATCGAACG TGGTCAGGTA CTGGCTAAGC CGGGCACCAT CAAGCCGCAC ACCAAGTTCG
  181 AATCTGAAGT GTACATTCTG TCCAAAGATG AAGGCGGCCG TCATACTCCG TTCTTCAAAG
  241 GCTACCGTCC GCAGTTCTAC TTCCGTACTA CTGACGTGAC TGGTACCATC GAACTGCCGG
  301 AAGGCGTAGA GATGGTAATG CCGGGCGACA ACATCAAAAT GGTTGTTACC CTGATCCACC
  361 CGATCGCGAT GGACGACGGT CTGCGTTTCG CAATCCGTGA AGGCGGCCGT ACCGTTGGCG
  421 CGGGCGTTGT AGCAAAAGTT CTGAGCGGGG CCTCTGGGGC CGCAGGTGGA GGCGGTTCAG
  481 GCGGAGGTGG CTCTATGTCT AAGACAGCAT CTTCGCGTAA CTCTCTCAGC GCACAATTGC
  541 GCCGAGCCGC GAACACAAGA ATTGAGGTTG AAGGTAACCT CGCACTTTCC ATTGCCAACG
  601 ATTTACTGTT GGCCTATGGT CAGTCGCCAT TTAACTCTGA GGCTGAGTGT ATTTCATTCA
  661 GCCCGAGATT CGACGGGACC CCGGATGACT TTAGGATAAA TTATCTTAAA GCCGAGATCA
  721 TGTCGAAGTA TGACGACTTC AGCCTANGTA TTGATACCGA AGCTGTTGCC TGGGAGAAGT
  781 TCCTGGCAGC AGAGGCTGAA TGTGCTTTAA CGAACGCTCG TCTCTATAGG CCTGACTACA
  841 GNGAGGANTT CNATTTCTCA CTGGGCGANT CNTGTATACA CATGGCTCGN ANAAAAATAG
  901 CCAAGCTAAT ANGANATGTT CCGNNNGNNG ANGGTATGNT GCGNCACTGN CNATTTTCTG
  961 GCGGNGCTNN NNCNNNNAAT NANNNNNNNN TACNGNNCAT CCGNNNCTNN NNGNTNNNNN
 1021 NNNNCNNNNN NNNNNNNNNN NNNNNNGNNN TNNNNNNNNN NNNNNNNTNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN
//
Primer: pBR_BamHI_R2
Region: araC
Sequence file: RDB17459_A9Foe.seq check
>D06471A6_A9Fo_4_pBR_BamHI_R2_H06_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNANGCGTCC GGCGTANAGG NNCTGCTCAT
   61 GTTTGACAGC TTATCATCGA TGCATAATGT GCCTGTCAAA TGGACGAAGC AGGGATTCTG
  121 CAAACCCTAT GCTACTCCGT CAAGCCGTCA ATTGTCTGAT TCGTTACCAA TTATGACAAC
  181 TTGACGGCTA CATCATTCAC TTTTTCTTCA CAACCGGCAC GGAACTCGCT CGGGCTGGCC
  241 CCGGTGCATT TTTTAAATAC CCGCGAGAAA TAGAGTTGAT CGTCAAAACC AACATTGCGA
  301 CCGACGGTGG CGATAGGCAT CCGGGTGGTG CTCAAAAGCA GCTTCGCCTG GCTGATACGT
  361 TGGTCCTCGC GCCAGCTTAA GACGCTAATC CCTAACTGCT GGCGGAAAAG ATGTGACAGA
  421 CGCGACGGCG ACAAGCAAAC ATGCTGTGCG ACGCTGGCGA TATCAAAATT GCTGTCTGCC
  481 AGGTGATCGC TGATGTACTG ACAAGCCTCG CGTACCCGAT TATCCATCGG TGGATGGAGC
  541 GACTCGTTAA TCGCTTCCAT GCGCCGCAGT AACAATTGCT CAAGCAGATT TATCGCCAGC
  601 AGCTCCGAAT AGCGCCCTTC CCCTTGCCCG GCGTTAATGA TTTGCCCAAA CAGGTCGCTG
  661 AAATGCGGCT GGTGCGCTTC ATCCGGGCGA AAGAACCCCG TATTGGCAAA TATTGACGGC
  721 CAGTTAAGCC ATTCATGCCA GTAGGCGCGC GGACGAAAGT AAACCCACTG GTGATACCAT
  781 TCGCGAGCCT CCGGATGACG ACCGNANTGA TGAATCTCTC CTGNCGGGAA CAGCAAAATA
  841 TCACCCNGGN CNGCAAACAA ATTCTCGTCC CTGANTTTTT CACCACCCCC CNGNNCGCNA
  901 ANGGNGANAT TGANAATATA ANCTTTTNNT TCCCAGCNGN NNGGNNGATA AAAAAAAANC
  961 NAGNNNANNN NNTNNNNCNN NNNNNNNNNN NNTNNNNNNN NNNNNNNNNN NNNNGNNNNN
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kita, H., Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit. J. Biosci. Bioeng. 101 (5): 421-426 (2006). PMID 16781472.

2019.12.20

GNP_filter3_RDBDEP_html_191130.pl