Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBAD33Ts-Tu-beta-3 (#RDB17459)

Bacterial expression vector of E. coli EF-Tu and EF-Ts, and beta-subunit of Q-beta replicase.

Clone info.	Bacterial expression vector of E. coli EF-Tu and EF-Ts, and beta-subunit of Q-beta replicase with His-tag. Schematic of this clone, see Fig.2A and B of Kita, H. et al., J. Biosci. Bioeng., 101 (5) :421-426, 2006.
Comment	The plasmids pBAD33 was kindly provided by Dr. S. Yasuda of the National Institute of Genetics, Japan. The plasmid ZpQbeta32 containing cDNA of the Qbeta phage genome was kindly provided by Dr. Y. Inokuchi. Expression was confirmed by the depositor with SDS-PAGE and CBB staining.
Vector backbone	pBAD33 (plasmid)
Selectable markers	Chloramphenicol
Gene/insert name	E. coli EF-Tu, EF-Ts genomic Bacteriophage Qbeta DNA polymerase genomic DNA
Depositor|Developer	Ichihashi, Norikazu |

 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biosci. Bioeng. 101(5), 421-426, 2006)

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB17459	pBAD33Ts-Tu-beta-3	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pBAD33Ts-Tu-beta-3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17459).

Reference section:

Kita, H., Cho, J., Matsuura, T., Nakaishi, T., Taniguchi, I., Ichikawa, T., Shima, Y., Urabe, I., Yomo, T., Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit. J. Biosci. Bioeng. 101 (5): 421-426 (2006). PMID 16781472. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17459_A9Fop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: araBADpro_F2 (Pr0801) Region: araBADpro, insert 5' (tsf) Sequence file: RDB17459_A9Foa.seq
>D06471A6_A9Fo_4_araBADpro_F2_G06_21_ABI24.ab1     1 NNNNNNNNNN NNCNGNNNNN NNNCNNCNTT GNNTATTTGC ACGGCGTCNC ACTTTGCTAT    61 GCCATAGCAT TTTTATCCNT AAGATTAGCG GATCCTACCT GACGCTTTTT ATCGCAACTC   121 TCTACTGTTT CTCCATACCC GTTTTTTTGG GCTAGCAGGA GGAATTCACC ATGGCTGAAA   181 TTACCGCATC CCTGGTAAAA GAGCTGCGTG AGCGTACTGG CGCAGGCATG ATGGATTGCA   241 AAAAAGCACT GACTGAAGCT AACGGCGACA TCGAGCTGGC AATCGAAAAC ATGCGTAAGT   301 CCGGTGCTAT TAAAGCAGCG AAAAAAGCAG GCAACGTTGC TGCTGACGGC GTGATCAAAA   361 CCAAAATCGA CGGCAACTAC GGCATCATTC TGGAAGTTAA CTGCCAGACT GACTTCGTTG   421 CAAAAGACGC TGGTTTCCAG GCGTTCGCAG ACAAAGTTCT GGACGCAGCT GTTGCTGGCA   481 AAATCACTGA CGTTGAAGTT CTGAAAGCAC AGTTCGAAGA AGAACGTGTT GCGCTGGTAG   541 CGAAAATTGG TGAAAACATC AACATTCGCC GCGTTGCTGC GCTGGAAGGC GACGTTCTGG   601 GTTCTTATCA GCACGGTGCG CGTATCGGCG TTCTGGTTGC TGCTAAAGGC GCTGACGAAG   661 AGCTGGTTAA ACACATCGCT ATGCACGTTG CTGCAAGCAA GCCAGAATTC ATCAAACCGG   721 AAGACGTATC CGCTGAAGTG GTAGAAAAAG AATACCAGGT ACAGCTGGAT ATCGCGATGC   781 AGTCTGGTAA GCCGAAAGAA ATCGCAGANA AAATGGNTGA AGGNCGCATG AAGANATTCA   841 CCGGCGAANT TTCTCTGACC GGTCAGCCGN TCGTTATGGA ANCNAGCAAA ACTGTTGGTC   901 AGCTGCTGAA AGANCATAAC GCTNAAGTGN NNGNNTTCNT CCNCTTCNAA NTGGGNGNAN   961 NNNATCNANA ANNTTGANNN TGNNTTTGCN NCANANNNNG CNNCNNNNNN CNNNNNNNNN 1021 N //
Primer: rrnBT1ter_F2 (Pr0531) Region: 6xHis, insert 3' (Qbeta) Sequence file: RDB17459_A9Fob.seq
>D06471A6_A9Fo_4_rrnBT1ter_F2_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NCNNNNNNTT TGATGCCTGG CANGTTCCCT    61 ACTCTCGCAT GGGGAGACCC CACNNNNNNA TCGGCGCTAC GGCGTTTCAC TTCTGAGTTC   121 GGCATGGGGT CAGGTGGGAC CACCGCGCTA CTGCCGCCAG GCAAATTCTG TTTTATCAGA   181 CCGCTTCTGC GTTCTGATTT AATCTGTATC AGGCTGAAAA TCTTCTCTCA TCCGCCAAAA   241 CAGCCAAGCT TCGTAAATTC TATACAAAAT TAGTGGTGGT GGTGGTGGTG CGCCTCGTGT   301 AGAGACGCAA CCTTCGTGCC CTGGAAGACC CCGTAGGGTG CCAGAACACG GCTACTGCAC   361 GCGATATACT GTATATCGAA TTTGCCGGTA GACCTGCTTA TCTTCGTAGG ATTACTCCTA   421 CAGATAAGCT GATCGATGGC AAATAGATCC GCAGAATCGC AACCCGATGG ACCCCTAAGA   481 GGCAACCCAT CGTTACTTTC CGAGAGACAA CGCGAGAAGA GGTCGTAGAG ATACGACCCC   541 AACTCAGCGC GCTCTCGGTC CCTTGTATGG TCCGTAATCA CCGGTACGTA CCGGATCCAC   601 CCGCGGTTTT TCGCGAAAGG ATTGATTAGG ACCGATCCGA CGAGGNNNNN NTCACCGTAA   661 CCATCAGGTA TAGTATTACG TNGNNNNTGT TTAGGCAGCA ACTTACGATA CTTGAGGTAC   721 ACAGAATGGG CCCTAGGNNC CCATACGCCG TCAATTGTGG CCCACCGATA TAGGTTATTC   781 NAAACCAGTA TTAAATCGGC AGGACTCACT ATACGGTGAN GTATGTANAA NGGAGTAACN   841 TCTACNCCNN AATAGTANTG NTTGCCGCAC GACTCTCNNN NCGGCCCNTN NGGAAAAAGN   901 CTTTTTNAGT ANNNGNNCGN NAAANNCAAC NTNCTNNAAA AACTNNCCNG NANGGGNNNG   961 GACTGCNCAG GANNGGTAAA ANTANNATCG TNNNNNNNTA AANNNGNGAN NNCNNNNNNA  1021 GTCTNAANNN NCAGTNNNNN NACAAACNGN AACGANGCNN ANNANNANNN NNAAANNANN 1081 NNNNNNNN //
Primer: RDB17459_F3 (Pr0728) Region: insert (tsf, tuf) Sequence file: RDB17459_A9Foc.seq
>D06471A6_A9Fo_4_RDB17459_F3_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTTG ANNNCGCATG NNAAATTCNC CGGCGAAGTT TCTCTGACCG    61 GTCAGCCGTT CGTTATGGAA CCAAGCAAAA CTGTTGGTCA GCTGCTGAAA GAGCATAACG   121 CTGAAGTGAC TGGCTTCATC CGCTTCGAAG TGGGTGAAGG CATCGAGAAA GTTGAGACTG   181 ACTTTGCAGC AGAAGTTGCT GCGATGTCCA AGCAGTCTCA TATGTCTAAA GAAAAGTTTG   241 AACGTACAAA ACCGCACGTT AACGTCGGTA CTATCGGCCA CGTTGACCAT GGTAAAACAA   301 CGCTGACCGC TGCAATCACT ACCGTACTGG CTAAAACCTA CGGCGGTGCT GCTCGCGCAT   361 TCGACCAGAT CGATAACGCG CCGGAAGAAA AAGCTCGTGG TATCACCATC AACACTTCTC   421 ACGTTGAATA CGACACCCCG ACCCGTCACT ACGCACACGT AGACTGCCCG GGGCACGCCG   481 ACTATGTTAA AAACATGATC ACCGGTGCTG CGCAGATGGA CGGCGCGATC CTGGTAGTTG   541 CTGCGACTGA CGGCCCGATG CCGCAGACTC GTGAGCACAT CCTGCTGGGT CGTCAGGTAG   601 GCGTTCCGTA CATCATCGTG TTCCTGAACA AATGCGACAT GGTTGATGAC NAAGAGCTGC   661 TGGAACTGGT TGAAATGGAA GTTCGTGAAC TTCTGTCTCA NTACGACTTC NCGGGCGACN   721 ACACTCCGAT CGTTCGNGNN NNNGCNCTGA AAGCGCTGGA NNGCGACGCN NAGTGGGAAN 781 NNNNNNNNN //
Primer: RDB17459_F2 (Pr0670) Region: insert (tuf, Qbeta) Sequence file: RDB17459_A9Fod.seq
>D06471A6_A9Fo_4_RDB17459_F2_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNNC TGTNCTGGCG TTGANTGTTC CGCAAACTGC    61 TGGACGAAGG CCGTGCTGGT GAGAACGTAG GTGTTCTGCT GCGTGGTATC AAACGTGAAG   121 AAATCGAACG TGGTCAGGTA CTGGCTAAGC CGGGCACCAT CAAGCCGCAC ACCAAGTTCG   181 AATCTGAAGT GTACATTCTG TCCAAAGATG AAGGCGGCCG TCATACTCCG TTCTTCAAAG   241 GCTACCGTCC GCAGTTCTAC TTCCGTACTA CTGACGTGAC TGGTACCATC GAACTGCCGG   301 AAGGCGTAGA GATGGTAATG CCGGGCGACA ACATCAAAAT GGTTGTTACC CTGATCCACC   361 CGATCGCGAT GGACGACGGT CTGCGTTTCG CAATCCGTGA AGGCGGCCGT ACCGTTGGCG   421 CGGGCGTTGT AGCAAAAGTT CTGAGCGGGG CCTCTGGGGC CGCAGGTGGA GGCGGTTCAG   481 GCGGAGGTGG CTCTATGTCT AAGACAGCAT CTTCGCGTAA CTCTCTCAGC GCACAATTGC   541 GCCGAGCCGC GAACACAAGA ATTGAGGTTG AAGGTAACCT CGCACTTTCC ATTGCCAACG   601 ATTTACTGTT GGCCTATGGT CAGTCGCCAT TTAACTCTGA GGCTGAGTGT ATTTCATTCA   661 GCCCGAGATT CGACGGGACC CCGGATGACT TTAGGATAAA TTATCTTAAA GCCGAGATCA   721 TGTCGAAGTA TGACGACTTC AGCCTANGTA TTGATACCGA AGCTGTTGCC TGGGAGAAGT   781 TCCTGGCAGC AGAGGCTGAA TGTGCTTTAA CGAACGCTCG TCTCTATAGG CCTGACTACA   841 GNGAGGANTT CNATTTCTCA CTGGGCGANT CNTGTATACA CATGGCTCGN ANAAAAATAG   901 CCAAGCTAAT ANGANATGTT CCGNNNGNNG ANGGTATGNT GCGNCACTGN CNATTTTCTG   961 GCGGNGCTNN NNCNNNNAAT NANNNNNNNN TACNGNNCAT CCGNNNCTNN NNGNTNNNNN 1021 NNNNCNNNNN NNNNN //
Primer: pBR_BamHI_R2 (Pr0141) Region: araC Sequence file: RDB17459_A9Foe.seq
>D06471A6_A9Fo_4_pBR_BamHI_R2_H06_24_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNANGCGTCC GGCGTANAGG NNCTGCTCAT    61 GTTTGACAGC TTATCATCGA TGCATAATGT GCCTGTCAAA TGGACGAAGC AGGGATTCTG   121 CAAACCCTAT GCTACTCCGT CAAGCCGTCA ATTGTCTGAT TCGTTACCAA TTATGACAAC   181 TTGACGGCTA CATCATTCAC TTTTTCTTCA CAACCGGCAC GGAACTCGCT CGGGCTGGCC   241 CCGGTGCATT TTTTAAATAC CCGCGAGAAA TAGAGTTGAT CGTCAAAACC AACATTGCGA   301 CCGACGGTGG CGATAGGCAT CCGGGTGGTG CTCAAAAGCA GCTTCGCCTG GCTGATACGT   361 TGGTCCTCGC GCCAGCTTAA GACGCTAATC CCTAACTGCT GGCGGAAAAG ATGTGACAGA   421 CGCGACGGCG ACAAGCAAAC ATGCTGTGCG ACGCTGGCGA TATCAAAATT GCTGTCTGCC   481 AGGTGATCGC TGATGTACTG ACAAGCCTCG CGTACCCGAT TATCCATCGG TGGATGGAGC   541 GACTCGTTAA TCGCTTCCAT GCGCCGCAGT AACAATTGCT CAAGCAGATT TATCGCCAGC   601 AGCTCCGAAT AGCGCCCTTC CCCTTGCCCG GCGTTAATGA TTTGCCCAAA CAGGTCGCTG   661 AAATGCGGCT GGTGCGCTTC ATCCGGGCGA AAGAACCCCG TATTGGCAAA TATTGACGGC   721 CAGTTAAGCC ATTCATGCCA GTAGGCGCGC GGACGAAAGT AAACCCACTG GTGATACCAT   781 TCGCGAGCCT CCGGATGACG ACCGNANTGA TGAATCTCTC CTGNCGGGAA CAGCAAAATA   841 TCACCCNGGN CNGCAAACAA ATTCTCGTCC CTGANTTTTT CACCACCCCC CNGNNCGCNA   901 ANGGNGANAT TGANAATATA ANCTTTTNNT TCCCAGCNGN NNGGNNGATA AAAAAAAANC 961 NAGNNNANNN NNTNNNNCNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Kita, H., Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit. J. Biosci. Bioeng. 101 (5): 421-426 (2006). PMID 16781472. [link to RRC of NBRP]

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Bacteriophage Qbeta DNA polymerase

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