Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBAD33betaH (#RDB17458)

Bacterial expression vector of beta-subunit of Q-beta replicase.

Clone info. Bacterial expression vector of beta-subunit of Q-beta replicase tagged with His at C-terminus.
Comment The plasmids pBAD33 was kindly provided by Dr. S. Yasuda of the National Institute of Genetics, Japan. The plasmid ZpQbeta32 containing cDNA of the Qbeta phage genome was kindly provided by Dr. Y. Inokuchi. Expression was confirmed by the depositor with SDS-PAGE and CBB staining.
Vector backbone pBAD33 (plasmid)
Selectable markers Chloramphenicol
Gene/insert name Bacteriophage Qbeta DNA polymerase genomic
Depositor|Developer Ichihashi, Norikazu |
 
Sequence (full) RDB17458zzk02.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biosci. Bioeng. 101(5), 421-426, 2006)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biosci. Bioeng. 101(5), 421-426, 2006)。

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RDB17458 pBAD33betaH DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBAD33betaH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17458).

Reference section:

Kita, H., Cho, J., Matsuura, T., Nakaishi, T., Taniguchi, I., Ichikawa, T., Shima, Y., Urabe, I., Yomo, T., Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit. J. Biosci. Bioeng. 101 (5): 421-426 (2006). PMID 16781472. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17458_A9Fop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: araBADpro_F1 (Pr0654)
Region: araBAD pro, insert 5'
Sequence file: RDB17458_A9Foa.seq check
>D06471A5_A9Fo_1_araBADpro_F1_F05_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCTTTT NNTCGCAACT CTCTACTGTT
   61 TCTCCNTACC CGTTTTTTTG GGCTAGCAGG AGGAATTCCA TATGTCTAAG ACAGCATCTT
  121 CGCGTAACTC TCTCAGCGCA CAATTGCGCC GAGCCGCGAA CACAAGAATT GAGGTTGAAG
  181 GTAACCTCGC ACTTTCCATT GCCAACGATT TACTGTTGGC CTATGGTCAG TCGCCATTTA
  241 ACTCTGAGGC TGAGTGTATT TCATTCAGCC CGAGATTCGA CGGGACCCCG GATGACTTTA
  301 GGATAAATTA TCTTAAAGCC GAGATCATGT CGAAGTATGA CGACTTCAGC CTAGGTATTG
  361 ATACCGAAGC TGTTGCCTGG GAGAAGTTCC TGGCAGCAGA GGCTGAATGT GCTTTAACGA
  421 ACGCTCGTCT CTATAGGCCT GACTACAGTG AGGATTTCAA TTTCTCACTG GGCGAGTCAT
  481 GTATACACAT GGCTCGTAGA AAAATAGCCA AGCTAATAGG AGATGTTCCG TCCGTTGAGG
  541 GTATGTTGCG TCACTGCCGA TTTTCTGGCG GTGCTACAAC AACGAATAAC CGTTCGTACG
  601 GTCATCCGTC CTTCAAGTTT GCGCTTCCGC AAGCGTGTAC GCCTCGGGCT TTGAAGTATG
  661 TTTTAGCTCT CAGAGCTTCT ACACATTTCG ATATCAGAAT TTCTGATATT AGCCCTTTTA
  721 ATAAAGCAGT TACTGTACCT AAGAACAGTA AGACAGATCG TTGTATTGCT ATCGAACCTG
  781 GTTGGAATAT GTTTTTCCNA CTNGGTATCG GTGGCATTCT ACGCGATCGG NTGCGTTGCT
  841 GGGGNTATCG ATCTGAATGA TCAGACGATA AATCAGCGCC GCGCTCACGA NNGNNNCNNT
  901 ACNNNTAACN TANCAANNGN NGATCTNNCA NNNGNNNGCN ATTCNATATC NCTNNNCNNN
961 NNNNNNN
//
Primer: rrnBT1ter_F2 (Pr0531)
Region: rrnBT1 ter, 6xHis, insert 3'
Sequence file: RDB17458_A9Fob.seq check
>D06471A5_A9Fo_1_rrnB1ter_F2_H05_23_ABI24.ab1
    1 NNNNNNNNNN TNNNNNNNNN NCTTTCGTTT NNTTTGATGC CTGGCAGTTC CCTACTCTCG
   61 CATGGGGAGA CCCCACACTA CCATCGGCGC TACGGCGTTT CACTTCTGAG TTCGGCATGG
  121 GGTCAGGTGG GACCACCGCG CTACTGCCGC CAGGCAAATT CTGTTTTATC AGACCGCTTC
  181 TGCGTTCTGA TTTAATCTGT ATCAGGCTGA AAATCTTCTC TCATCCGCCA AAACAGCCAA
  241 GCTTCGTAAA TTCTATACAA AATTAGTGGT GGTGGTGGTG GTGAGATCTC GCCTCGTGTA
  301 GAGACGCAAC CTTCGTGCCC TGGAAGACCC CGTAGGGTGC CAGAACACGG CTACTGCACG
  361 CGATATACTG TATATCGAAT TTGCCGGTAG ACCTGCTTAT CTTCGTAGGA TTACTCCTAC
  421 AGATAAGCTG ATCGATGGCA AATAGATCCG CAGAATCGCA ACCCGATGGA CCCCTAAGAG
  481 GCAACCCATC GTTACTTTCC GAGAGACAAC GCGAGAAGAG GTCGTAGAGA TACGACCCCA
  541 ACTCAGCGCG CTCTCGGTCC CTTGTATGGT CCGTAATCAC CGGTACGTAC CGGATCCACC
  601 CGCGGTTTTT CGCGAAAGGA TTGATTAGGA CCGATCCGAC GAGGGCACCA TCACCGTAAC
  661 CATCAGGTAT AGTATTACGT TGCAGCTGTT TAGGCAGCAA CTTACGATAC TTGAGGTACA
  721 CAGAATGGGC CCTANGATCC CATACGCCGT CAATTGTGGC CCACCGATAT AGGTTATTCA
  781 AAACCAGTAT TAAATCGGCA GGACTCACTA TACGGTGACG TATGTAAAAG GGAGTAACAT
  841 CTACGCCNGA NTAGTAGTGC TTGCCGCACG ACTCTCTGAA CGGCCCCTCG GAAAAAGNCT
  901 TTTTTAGTAT TGGNCGNNAA NNNNACNTAC TTNAAANNTT CCNNGNANGN NAGGNNNTGC
961 NNNNGNANNG NAAANTNNTA TCGNNNNNNN NANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kita, H., Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit. J. Biosci. Bioeng. 101 (5): 421-426 (2006). PMID 16781472. [link to RRC of NBRP]