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pBAD33betaH

Bacterial expression vector of beta-subunit of Q-beta replicase.

Catalog number RDB17458
Resource name pBAD33betaH
Clone info. Bacterial expression vector of beta-subunit of Q-beta replicase tagged with His at C-terminus.
The plasmids pBAD33 was kindly provided by Dr. S. Yasuda of the National Institute of Genetics, Japan. The plasmid ZpQbeta32 containing cDNA of the Qbeta phage genome was kindly provided by Dr. Y. Inokuchi. Expression was confirmed by the depositor with SDS-PAGE and CBB staining.
Vector backbone pBAD33 (plasmid)
Selectable markers Chloramphenicol
Gene/insert name Bacteriophage DNA polymerase genomic
Depositor Ichihashi, Norikazu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biosci. Bioeng. 101(5), 421-426, 2006)
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17458 pBAD33betaH Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles


2019.08.01

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