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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pUC-phi29DNAP_clone6

Plasmid clone of the transcription and translation-coupled DNA replication (TTcDR) system, harboring phi29 DNA polymerase coding region and loxP site.

Catalog number RDB17455
Resource name pUC-phi29DNAP_clone6
Clone info. Plasmid clone of the transcription and translation-coupled DNA replication (TTcDR) system, harboring phi29 DNA polymerase coding region and loxP site. Please refer Fig.1 of Sakatani, Y. et al., Sci. Rep., 8 (1) :13089, 2018. Clone 6 contains mutations contributing to improved DNA replication and mutated loxP (C2022T).
Vector backbone pUC19 (plasmid)
Selectable markers Amp
Gene/insert name Bacteriophage phi29 DNA polymerase genomic
Depositor|Developer Ichihashi, Norikazu |
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External Database
Bacteriophage phi29 DNA polymerase

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sakatani, Y. et al., Sci. Rep. 8(1), 13089, 2018)
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sakatani, Y. et al., Sci. Rep. 8(1), 13089, 2018)。
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            RDB17455 pUC-phi29DNAP_clone6 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pUC-phi29DNAP_clone6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17455).

            Reference section:

            Sakatani, Y., Yomo, T., Ichihashi, N., Self-replication of circular DNA by a self-encoded DNA polymerase through rolling-circle replication and recombination. Sci. Rep. 8 (1): 13089 (2018). PMID 30166584.

            Further references such as user reports and related articles (go to bottom)


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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17455_A9Fop1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40 (Pr0410)
            Region: insert 5'(T7 pro,lac op, RBS,phi29 DNAP 5')
            Sequence file: RDB17455_A9Foa.seq check
            >D06471A2_A9Fo_1_M13_-40_D04_10_ABI24.ab1
                1 CGGCGGGTAA TCGAGCTCGG TACCCGGGGA TCCTCTAGAG TCGACCTGCA GGCATGCTAA
               61 TACGACTCAC TATAGGGGGG TTGTGAGCGG ATAACAATCC CCCCCTAGAA ATAATTTTGT
              121 TTAACTTTAA GAAGGAGATA TACATATGCC GAGAAAGATG TATAGTTGTG ACTTTGAGAC
              181 AACTACTAAA GTGGAAGACT GTAGGGTATG GGCGTATGGT TATATGAATA TAGAAGATCA
              241 CAGTGAGTAC AAAATAGGTA ATAGCCTGGA TGAGTTTATG GCGTGGGTGT TGAAGGTACA
              301 AGCTGATCTA TATTTCCATA ACCTCAAATT TGACGGAGCT TTTATCATTA ACTGGTTGGA
              361 ACGTAGTGGT TTTAAGTGGT CGGCTGACGG ATTGCCAAAC ACATATAATA CGATCATATC
              421 TCGCATGGGA CAATGGTACA TGATTGATAT ATGTTTAGGC TACAAAGGGA AACGTAAGAT
              481 ACATACAGTG ATATATGACA GCTTAAAGAA ACTACCGTTT CCTGTTAAGA AGATAGCTAA
              541 AGACTTTAAA CTAACTGTTC TTAAAGGTGA TATTGATTAC CACAAAGAAA GACCAGTCGG
              601 CTATAAGATA ACACCCGAAG AATACGCCTA TATTAAAAAC GATATTCAGA TTATTGCGGA
              661 AGCTCTGTTA ATTCAATTCA AGCAAGGTTT AGACCGGATG ACAGCAGGCA GTGACAGTCT
              721 AAAAGGTTTC AAGGATATTA TAACCACTAA GAAATTCAAA AAGGTATTTC CTACATTGAG
              781 TCTTGGACTC GATAAGGAAG TGAGATACGC CTATAGAGGT GGTTTTACAT GGTTAAATGA
              841 TAGGTTCAAA GAAAAGAAAT CGGAGAAGGC ATGGTCTTCG ATGTTAATAG TCTATATCCT
              901 GCACAGATGT ATAGCCGTCT CCTTCCATAC GGTGAACCTA TAGTATTCGA GGGTAAATAC
              961 GTTTGGGACG AAGATTACCC ACTACACATA CAGCATATCA GATGTGAGTT CGAAATTGAA
             1021 AGAGGGCTAT ATACCCACTA TACAGAATAA AAAGAAGTAA GGTTTTTTAT AAATGGTTAT
            1081 TGGAGTTACA TAAAAAGCAG CGGTCGGGGG GAGAATTAGG TCG
            //
            Primer: pBAC_lac_R (Pr0545)
            Region: insert (loxP,phi29 DNAP 3')
            Sequence file: RDB17455_A9Fob.seq check
            >D06471A2_A9Fo_1_pBAC_lac_R_E04_13_ABI24.ab1
                1 GCAGGCAGCG CTAGTTATTG CTCAGCGGAT AACTTCATAT AATGTATGCT ATACGAAGTT
               61 ATTGACAGCA GCCAACTCAG CTTCCTTTCG GGCTTCGTTA GCAGCCGGAT CTCAGTGGTG
              121 GTGGTGGTGC TCGAGTGCGG CCGCAAGCTT GTTGACGGAG CTCGAATTCA TTATTTGATT
              181 GTGAATGTGT TATCAACCAG AACCACCCCG CCCGGCACTT GCACAGGCTT AGGCGTCATT
              241 TTCCGACTGA ATCCGACTTT GAAATTCTCA AACGTAACCT CTTTCTTAAT CTTGTCAGTC
              301 ATTCCCGCAC ATTTAACACT AAATTTTATA TCAGTGTAAT CATCTGGACT ACCTTCTACT
              361 AACTTACCAT CTACTTCTTT CATATAGATG TCTTGTATAT AGGTCTTCTG TCTCAAATAT
              421 TTAGCTCTTT TGAATGTACT TTCATGTGCC CAGTATCCCA ATTTCTTAGG GTCAACTATA
              481 TCTTTTATTA CATCAGGTAT CTCTGTACCC GTTAAATGTA TGCTGTCAGT ATCACAGTAT
              541 ATTATCCGAT CATAACAAGC CTGTGCCGCT GTAATTGTCG TGTATCTAGC CCATGCAGTG
              601 ATGAAAACGC CCATAGGTGT ATAAACAGGG TCTTTTGTTT CCTCTTCTCC AAGTCTGAAA
              661 CCTAGCGCCC CATTCTCTTT TAAATAAGGG ACTTTCCCTG TAACATCAGG GTTACTAGCG
              721 AATTTACCGT ATAGACTGTT TAACATCAGT TTTGCTAGTT GCTTGATCGC TCCTTCTGAT
              781 GTCGTCTTGA TGTACGTCCA TTTATCTATA AAATCTTTAA ACAAACCTGT AGTTGCTTTA
              841 AATTTTAAGC CGCTGATATA TTCAACGTTA TATAAATCAT AGTGTTCTTT CATTAATTCT
              901 AGGTCTACAT TTGACAACCA GAGGTCGGCT ATCTCCCCGC CGCTACTTTT TTAGGTACTC
              961 ATTACCTTTA TAAAACCTAC TTCTTTTTAT CTGTATAGTG GGTATATAGC CCTCTTTCAA
             1021 TTCGAACTCA CATCTGATAT GCTGTATGTG TTAGTGGTAA TCTTCGTCCC CAAACGGTAT
            1081 TAACCCTCGG AATACCTATA GGATTCACCG TTATTGGAAG GGGAGAC
            //
            Primer: pUC-CAPbs_up_F (Pr0536)
            Region: insert (loxP 5',phi29 DNAP 3')
            Sequence file: RDB17455_A9Foc.seq check
            >D06471A2_A9Fo_1_pUC-CAPbs_up_F_F04_16_ABI24.ab1
                1 ACTGGAGGGG AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG
               61 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GCGAGCGGAT
              121 AATAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCGCTAGTT
              181 ATTGCTCAGC GGATAACTTC ATATAATGTA TGCTATACGA AGTTATTGAC AGCAGCCAAC
              241 TCAGCTTCCT TTCGGGCTTC GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGCTCGAGT
              301 GCGGCCGCAA GCTTGTTGAC GGAGCTCGAA TTCATTATTT GATTGTGAAT GTGTTATCAA
              361 CCAGAACCAC CCCGCCCGGC ACTTGCACAG GCTTAGGCGT CATTTTCCGA CTGAATCCGA
              421 CTTTGAAATT CTCAAACGTA ACCTCTTTCT TAATCTTGTC AGTCATTCCC CCCCCATTTA
              481 ACACTAAATT TTATATCGTG GAATTATCTG GAATACCTTT CTCCTAACTT ACCATCTACT
              541 TCTTTCAAAA AAGGTCTGGA AAAGGTCTTC TGCCCCAAAA TTTAACCCTT TTGAAGGGCT
              601 TTTCGGGGCC CGTTCCCCAA TTTCTAGGGG CAACAATTTT TTTTTTACAT CAGGGATCTC
              661 TGCACCCGTT AAAGGGTGCT GCCGATACCC CATATATTAT CGATATAAAC CGCCCGGCCC
              721 GTTGATTTGT GGGTTTTCCC CGGGGAGAAA ACCCCCATGT GTAAACGGCT TTTTTTCCTT
              781 CCCAATAAAC CGCCCCTTTT TTAAAAGGGA TCTCTCGGGA CGGGGAAAAA AAGATCTGAC
              841 ACAGACGAAT ATATCATCGG TGTCAGATGC TGTTCCCGCG TCATGGGGAC GTCTTCTTGT
              901 GTGAGTCACT ACTATAATAC GAAAACTTGG AGCAGCGACG AGTTGATACG CTCAAATAAT
            961 TAAG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Sakatani, Y., Self-replication of circular DNA by a self-encoded DNA polymerase through rolling-circle replication and recombination. Sci. Rep. 8 (1): 13089 (2018). PMID 30166584.

            2023.05.01

            GNP_filter3_RDBDEP_html_230501.pl