Please review the QC test results indicated by icon below as well as clone information before placing your order.

pUC-phi29DNAP_clone6

Plasmid clone of the transcription and translation-coupled DNA replication (TTcDR) system, harboring phi29 DNA polymerase coding region and loxP site.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sakatani, Y. et al., Sci. Rep. 8(1), 13089, 2018)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sakatani, Y. et al., Sci. Rep. 8(1), 13089, 2018)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17455_A9Fop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Region: insert 5'(T7 pro,lac op, RBS,phi29 DNAP 5') Sequence file: RDB17455_A9Foa.seq
>D06471A2_A9Fo_1_M13_-40_D04_10_ABI24.ab1     1 CGGCGGGTAA TCGAGCTCGG TACCCGGGGA TCCTCTAGAG TCGACCTGCA GGCATGCTAA    61 TACGACTCAC TATAGGGGGG TTGTGAGCGG ATAACAATCC CCCCCTAGAA ATAATTTTGT   121 TTAACTTTAA GAAGGAGATA TACATATGCC GAGAAAGATG TATAGTTGTG ACTTTGAGAC   181 AACTACTAAA GTGGAAGACT GTAGGGTATG GGCGTATGGT TATATGAATA TAGAAGATCA   241 CAGTGAGTAC AAAATAGGTA ATAGCCTGGA TGAGTTTATG GCGTGGGTGT TGAAGGTACA   301 AGCTGATCTA TATTTCCATA ACCTCAAATT TGACGGAGCT TTTATCATTA ACTGGTTGGA   361 ACGTAGTGGT TTTAAGTGGT CGGCTGACGG ATTGCCAAAC ACATATAATA CGATCATATC   421 TCGCATGGGA CAATGGTACA TGATTGATAT ATGTTTAGGC TACAAAGGGA AACGTAAGAT   481 ACATACAGTG ATATATGACA GCTTAAAGAA ACTACCGTTT CCTGTTAAGA AGATAGCTAA   541 AGACTTTAAA CTAACTGTTC TTAAAGGTGA TATTGATTAC CACAAAGAAA GACCAGTCGG   601 CTATAAGATA ACACCCGAAG AATACGCCTA TATTAAAAAC GATATTCAGA TTATTGCGGA   661 AGCTCTGTTA ATTCAATTCA AGCAAGGTTT AGACCGGATG ACAGCAGGCA GTGACAGTCT   721 AAAAGGTTTC AAGGATATTA TAACCACTAA GAAATTCAAA AAGGTATTTC CTACATTGAG   781 TCTTGGACTC GATAAGGAAG TGAGATACGC CTATAGAGGT GGTTTTACAT GGTTAAATGA   841 TAGGTTCAAA GAAAAGAAAT CGGAGAAGGC ATGGTCTTCG ATGTTAATAG TCTATATCCT   901 GCACAGATGT ATAGCCGTCT CCTTCCATAC GGTGAACCTA TAGTATTCGA GGGTAAATAC   961 GTTTGGGACG AAGATTACCC ACTACACATA CAGCATATCA GATGTGAGTT CGAAATTGAA  1021 AGAGGGCTAT ATACCCACTA TACAGAATAA AAAGAAGTAA GGTTTTTTAT AAATGGTTAT 1081 TGGAGTTACA TAAAAAGCAG CGGTCGGGGG GAGAATTAGG TCG //
Primer: pBAC_lac_R Region: insert (loxP,phi29 DNAP 3') Sequence file: RDB17455_A9Fob.seq
>D06471A2_A9Fo_1_pBAC_lac_R_E04_13_ABI24.ab1     1 GCAGGCAGCG CTAGTTATTG CTCAGCGGAT AACTTCATAT AATGTATGCT ATACGAAGTT    61 ATTGACAGCA GCCAACTCAG CTTCCTTTCG GGCTTCGTTA GCAGCCGGAT CTCAGTGGTG   121 GTGGTGGTGC TCGAGTGCGG CCGCAAGCTT GTTGACGGAG CTCGAATTCA TTATTTGATT   181 GTGAATGTGT TATCAACCAG AACCACCCCG CCCGGCACTT GCACAGGCTT AGGCGTCATT   241 TTCCGACTGA ATCCGACTTT GAAATTCTCA AACGTAACCT CTTTCTTAAT CTTGTCAGTC   301 ATTCCCGCAC ATTTAACACT AAATTTTATA TCAGTGTAAT CATCTGGACT ACCTTCTACT   361 AACTTACCAT CTACTTCTTT CATATAGATG TCTTGTATAT AGGTCTTCTG TCTCAAATAT   421 TTAGCTCTTT TGAATGTACT TTCATGTGCC CAGTATCCCA ATTTCTTAGG GTCAACTATA   481 TCTTTTATTA CATCAGGTAT CTCTGTACCC GTTAAATGTA TGCTGTCAGT ATCACAGTAT   541 ATTATCCGAT CATAACAAGC CTGTGCCGCT GTAATTGTCG TGTATCTAGC CCATGCAGTG   601 ATGAAAACGC CCATAGGTGT ATAAACAGGG TCTTTTGTTT CCTCTTCTCC AAGTCTGAAA   661 CCTAGCGCCC CATTCTCTTT TAAATAAGGG ACTTTCCCTG TAACATCAGG GTTACTAGCG   721 AATTTACCGT ATAGACTGTT TAACATCAGT TTTGCTAGTT GCTTGATCGC TCCTTCTGAT   781 GTCGTCTTGA TGTACGTCCA TTTATCTATA AAATCTTTAA ACAAACCTGT AGTTGCTTTA   841 AATTTTAAGC CGCTGATATA TTCAACGTTA TATAAATCAT AGTGTTCTTT CATTAATTCT   901 AGGTCTACAT TTGACAACCA GAGGTCGGCT ATCTCCCCGC CGCTACTTTT TTAGGTACTC   961 ATTACCTTTA TAAAACCTAC TTCTTTTTAT CTGTATAGTG GGTATATAGC CCTCTTTCAA  1021 TTCGAACTCA CATCTGATAT GCTGTATGTG TTAGTGGTAA TCTTCGTCCC CAAACGGTAT 1081 TAACCCTCGG AATACCTATA GGATTCACCG TTATTGGAAG GGGAGAC //
Primer: pUC-CAPbs_up_F Region: insert (loxP 5',phi29 DNAP 3') Sequence file: RDB17455_A9Foc.seq
>D06471A2_A9Fo_1_pUC-CAPbs_up_F_F04_16_ABI24.ab1     1 ACTGGAGGGG AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG    61 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GCGAGCGGAT   121 AATAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCGCTAGTT   181 ATTGCTCAGC GGATAACTTC ATATAATGTA TGCTATACGA AGTTATTGAC AGCAGCCAAC   241 TCAGCTTCCT TTCGGGCTTC GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGCTCGAGT   301 GCGGCCGCAA GCTTGTTGAC GGAGCTCGAA TTCATTATTT GATTGTGAAT GTGTTATCAA   361 CCAGAACCAC CCCGCCCGGC ACTTGCACAG GCTTAGGCGT CATTTTCCGA CTGAATCCGA   421 CTTTGAAATT CTCAAACGTA ACCTCTTTCT TAATCTTGTC AGTCATTCCC CCCCCATTTA   481 ACACTAAATT TTATATCGTG GAATTATCTG GAATACCTTT CTCCTAACTT ACCATCTACT   541 TCTTTCAAAA AAGGTCTGGA AAAGGTCTTC TGCCCCAAAA TTTAACCCTT TTGAAGGGCT   601 TTTCGGGGCC CGTTCCCCAA TTTCTAGGGG CAACAATTTT TTTTTTACAT CAGGGATCTC   661 TGCACCCGTT AAAGGGTGCT GCCGATACCC CATATATTAT CGATATAAAC CGCCCGGCCC   721 GTTGATTTGT GGGTTTTCCC CGGGGAGAAA ACCCCCATGT GTAAACGGCT TTTTTTCCTT   781 CCCAATAAAC CGCCCCTTTT TTAAAAGGGA TCTCTCGGGA CGGGGAAAAA AAGATCTGAC   841 ACAGACGAAT ATATCATCGG TGTCAGATGC TGTTCCCGCG TCATGGGGAC GTCTTCTTGT   901 GTGAGTCACT ACTATAATAC GAAAACTTGG AGCAGCGACG AGTTGATACG CTCAAATAAT 961 TAAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.10.08

GNP_filter3_RDBDEP_html_210618.pl