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pUC-phi29DNAP_clone6 (#RDB17455)

Plasmid clone of the transcription and translation-coupled DNA replication (TTcDR) system, harboring phi29 DNA polymerase coding region and loxP site.

Clone info. Plasmid clone of the transcription and translation-coupled DNA replication (TTcDR) system, harboring phi29 DNA polymerase coding region and loxP site. Please refer Fig.1 of Sakatani, Y. et al., Sci. Rep., 8 (1) :13089, 2018. Clone 6 contains mutations contributing to improved DNA replication and mutated loxP (C2022T).
Vector backbone pUC19 (plasmid)
Selectable markers Amp
Gene/insert name Bacteriophage phi29 DNA polymerase genomic
Depositor|Developer Ichihashi, Norikazu |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sakatani, Y. et al., Sci. Rep. 8(1), 13089, 2018)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sakatani, Y. et al., Sci. Rep. 8(1), 13089, 2018)。

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RDB17455 pUC-phi29DNAP_clone6 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pUC-phi29DNAP_clone6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17455).

Reference section:

Sakatani, Y., Yomo, T., Ichihashi, N., Self-replication of circular DNA by a self-encoded DNA polymerase through rolling-circle replication and recombination. Sci. Rep. 8 (1): 13089 (2018). PMID 30166584. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17455_A9Fop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410)
Region: insert 5'(T7 pro,lac op, RBS,phi29 DNAP 5')
Sequence file: RDB17455_A9Foa.seq check
>D06471A2_A9Fo_1_M13_-40_D04_10_ABI24.ab1
    1 CGGCGGGTAA TCGAGCTCGG TACCCGGGGA TCCTCTAGAG TCGACCTGCA GGCATGCTAA
   61 TACGACTCAC TATAGGGGGG TTGTGAGCGG ATAACAATCC CCCCCTAGAA ATAATTTTGT
  121 TTAACTTTAA GAAGGAGATA TACATATGCC GAGAAAGATG TATAGTTGTG ACTTTGAGAC
  181 AACTACTAAA GTGGAAGACT GTAGGGTATG GGCGTATGGT TATATGAATA TAGAAGATCA
  241 CAGTGAGTAC AAAATAGGTA ATAGCCTGGA TGAGTTTATG GCGTGGGTGT TGAAGGTACA
  301 AGCTGATCTA TATTTCCATA ACCTCAAATT TGACGGAGCT TTTATCATTA ACTGGTTGGA
  361 ACGTAGTGGT TTTAAGTGGT CGGCTGACGG ATTGCCAAAC ACATATAATA CGATCATATC
  421 TCGCATGGGA CAATGGTACA TGATTGATAT ATGTTTAGGC TACAAAGGGA AACGTAAGAT
  481 ACATACAGTG ATATATGACA GCTTAAAGAA ACTACCGTTT CCTGTTAAGA AGATAGCTAA
  541 AGACTTTAAA CTAACTGTTC TTAAAGGTGA TATTGATTAC CACAAAGAAA GACCAGTCGG
  601 CTATAAGATA ACACCCGAAG AATACGCCTA TATTAAAAAC GATATTCAGA TTATTGCGGA
  661 AGCTCTGTTA ATTCAATTCA AGCAAGGTTT AGACCGGATG ACAGCAGGCA GTGACAGTCT
  721 AAAAGGTTTC AAGGATATTA TAACCACTAA GAAATTCAAA AAGGTATTTC CTACATTGAG
  781 TCTTGGACTC GATAAGGAAG TGAGATACGC CTATAGAGGT GGTTTTACAT GGTTAAATGA
  841 TAGGTTCAAA GAAAAGAAAT CGGAGAAGGC ATGGTCTTCG ATGTTAATAG TCTATATCCT
  901 GCACAGATGT ATAGCCGTCT CCTTCCATAC GGTGAACCTA TAGTATTCGA GGGTAAATAC
  961 GTTTGGGACG AAGATTACCC ACTACACATA CAGCATATCA GATGTGAGTT CGAAATTGAA
 1021 AGAGGGCTAT ATACCCACTA TACAGAATAA AAAGAAGTAA GGTTTTTTAT AAATGGTTAT
1081 TGGAGTTACA TAAAAAGCAG CGGTCGGGGG GAGAATTAGG TCG
//
Primer: pBAC_lac_R (Pr0545)
Region: insert (loxP,phi29 DNAP 3')
Sequence file: RDB17455_A9Fob.seq check
>D06471A2_A9Fo_1_pBAC_lac_R_E04_13_ABI24.ab1
    1 GCAGGCAGCG CTAGTTATTG CTCAGCGGAT AACTTCATAT AATGTATGCT ATACGAAGTT
   61 ATTGACAGCA GCCAACTCAG CTTCCTTTCG GGCTTCGTTA GCAGCCGGAT CTCAGTGGTG
  121 GTGGTGGTGC TCGAGTGCGG CCGCAAGCTT GTTGACGGAG CTCGAATTCA TTATTTGATT
  181 GTGAATGTGT TATCAACCAG AACCACCCCG CCCGGCACTT GCACAGGCTT AGGCGTCATT
  241 TTCCGACTGA ATCCGACTTT GAAATTCTCA AACGTAACCT CTTTCTTAAT CTTGTCAGTC
  301 ATTCCCGCAC ATTTAACACT AAATTTTATA TCAGTGTAAT CATCTGGACT ACCTTCTACT
  361 AACTTACCAT CTACTTCTTT CATATAGATG TCTTGTATAT AGGTCTTCTG TCTCAAATAT
  421 TTAGCTCTTT TGAATGTACT TTCATGTGCC CAGTATCCCA ATTTCTTAGG GTCAACTATA
  481 TCTTTTATTA CATCAGGTAT CTCTGTACCC GTTAAATGTA TGCTGTCAGT ATCACAGTAT
  541 ATTATCCGAT CATAACAAGC CTGTGCCGCT GTAATTGTCG TGTATCTAGC CCATGCAGTG
  601 ATGAAAACGC CCATAGGTGT ATAAACAGGG TCTTTTGTTT CCTCTTCTCC AAGTCTGAAA
  661 CCTAGCGCCC CATTCTCTTT TAAATAAGGG ACTTTCCCTG TAACATCAGG GTTACTAGCG
  721 AATTTACCGT ATAGACTGTT TAACATCAGT TTTGCTAGTT GCTTGATCGC TCCTTCTGAT
  781 GTCGTCTTGA TGTACGTCCA TTTATCTATA AAATCTTTAA ACAAACCTGT AGTTGCTTTA
  841 AATTTTAAGC CGCTGATATA TTCAACGTTA TATAAATCAT AGTGTTCTTT CATTAATTCT
  901 AGGTCTACAT TTGACAACCA GAGGTCGGCT ATCTCCCCGC CGCTACTTTT TTAGGTACTC
  961 ATTACCTTTA TAAAACCTAC TTCTTTTTAT CTGTATAGTG GGTATATAGC CCTCTTTCAA
 1021 TTCGAACTCA CATCTGATAT GCTGTATGTG TTAGTGGTAA TCTTCGTCCC CAAACGGTAT
1081 TAACCCTCGG AATACCTATA GGATTCACCG TTATTGGAAG GGGAGAC
//
Primer: pUC-CAPbs_up_F (Pr0536)
Region: insert (loxP 5',phi29 DNAP 3')
Sequence file: RDB17455_A9Foc.seq check
>D06471A2_A9Fo_1_pUC-CAPbs_up_F_F04_16_ABI24.ab1
    1 ACTGGAGGGG AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG
   61 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GCGAGCGGAT
  121 AATAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCGCTAGTT
  181 ATTGCTCAGC GGATAACTTC ATATAATGTA TGCTATACGA AGTTATTGAC AGCAGCCAAC
  241 TCAGCTTCCT TTCGGGCTTC GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGCTCGAGT
  301 GCGGCCGCAA GCTTGTTGAC GGAGCTCGAA TTCATTATTT GATTGTGAAT GTGTTATCAA
  361 CCAGAACCAC CCCGCCCGGC ACTTGCACAG GCTTAGGCGT CATTTTCCGA CTGAATCCGA
  421 CTTTGAAATT CTCAAACGTA ACCTCTTTCT TAATCTTGTC AGTCATTCCC CCCCCATTTA
  481 ACACTAAATT TTATATCGTG GAATTATCTG GAATACCTTT CTCCTAACTT ACCATCTACT
  541 TCTTTCAAAA AAGGTCTGGA AAAGGTCTTC TGCCCCAAAA TTTAACCCTT TTGAAGGGCT
  601 TTTCGGGGCC CGTTCCCCAA TTTCTAGGGG CAACAATTTT TTTTTTACAT CAGGGATCTC
  661 TGCACCCGTT AAAGGGTGCT GCCGATACCC CATATATTAT CGATATAAAC CGCCCGGCCC
  721 GTTGATTTGT GGGTTTTCCC CGGGGAGAAA ACCCCCATGT GTAAACGGCT TTTTTTCCTT
  781 CCCAATAAAC CGCCCCTTTT TTAAAAGGGA TCTCTCGGGA CGGGGAAAAA AAGATCTGAC
  841 ACAGACGAAT ATATCATCGG TGTCAGATGC TGTTCCCGCG TCATGGGGAC GTCTTCTTGT
  901 GTGAGTCACT ACTATAATAC GAAAACTTGG AGCAGCGACG AGTTGATACG CTCAAATAAT
961 TAAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sakatani, Y., Self-replication of circular DNA by a self-encoded DNA polymerase through rolling-circle replication and recombination. Sci. Rep. 8 (1): 13089 (2018). PMID 30166584. [link to RRC of NBRP]