Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CDCA7 px330 (#RDB17380)

Expression vector of guide RNA for targeting human CDCA7 exon 7.

Alternative name CDCA7 exon7 pX330-U6-Chimeric_BB-CBh-hSpCas9
Clone info. Expression vector of guide RNA for targeting human cell division cycle associated 7 (CDCA7) exon 7 (5'-TCAGAAGACTATTGATACCA-3') and human codon-optimized SpCas9.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r (E. coli)
Gene/insert name human CDCA7 dsOligo
Depositor|Developer Unoki, Motoko |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17380 CDCA7 px330 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CDCA7 px330 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17380).

Reference section:

Unoki, M., Funabiki, H., Velasco, G., Francastel, C., Sasaki, H., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17380_A9Fhp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'element reverse (Pr0108)
Region: U6 pro, gRNA (CDCA7) gRNA scaffold, CBh pro 5'
Sequence file: RDB17380_A9Fha.seq check
>D06399A7_A9Fh_2_SV40pA3'element reverse_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG CNNGCAACGC GGCCTTTTTA CGGTTCCTGG
   61 CCTTTTGCTG GCCTTTTGCT CACATGTGAG GGCCTATTTC CCATGATTCC TTCATATTTG
  121 CATATACGAT ACAAGGCTGT TAGAGAGATA ATTGGAATTA ATTTGACTGT AAACACAAAG
  181 ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA
  241 AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC
  301 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGTGG TATCAATAGT CTTCTGAGTT
  361 TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC
  421 ACCGAGTCGG TGCTTTTTTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC
  481 GTTTTTAGCG CGTGCGCCAA TTCTGCAGAC AAATGGCTCT AGAGGTACCC GTTACATAAC
  541 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAG
  601 TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC
  661 ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG
  721 GTAAATGGCC CGCCTGGCAT TGTGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC
  781 AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTCGA NGTGAGCCCC ACGTTCTGCT
  841 TCACTCTCCC CATCTCCCCC CCCCNNCCCA CCCCCAATTT TGTATTTATT TATTTTTTTA
901 ATTATTTTGT GCAGCGATGG GGGCGGGGGG GGGGGGGGGG GGNNNNNNNN NN
//
Primer: Cas9-N_R1 (Pr0650)
Region: Cas9 5', SV40 NLS, 3xFLAG, CBh pro 3'
Sequence file: RDB17380_A9Fhb.seq check
>D06399A7_A9Fh_2_Cas9-N_R1_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNGNNNNGN NNNTTGNNNT CGTCGGTGNN CACGGCCCAG CCCACAGAGT
   61 TGGTGCCGAT GTCCAGGCCG ATGCTGTACT TCTTGTCGGC TGCTGGGACT CCGTGGATAC
  121 CGACCTTCCG CTTCTTCTTT GGGGCCATCT TATCGTCATC GTCTTTGTAA TCAATATCAT
  181 GATCCTTGTA GTCTCCGTCG TGGTCCTTAT AGTCCATGGT GGCACCGGTC CAACCTGAAA
  241 AAAAGTGATT TCAGGCAGGT GCTCCAGGTA ATTAAACATT AATACCCCAC CAACCAACCA
  301 TCCCTTAAAC CCTTACCTCT TGCTCAGCTA ATTACAGCCC GGAGGAGAAG GGCCGTCCCG
361 CCCGCTCACC TGTGGGAGTA ACGCGGTCAG TCAGAGCCGG GGCGGGCGGC GCGAGGNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH p(A), nucleoplasmin NLS, Cas9 3'
Sequence file: RDB17380_A9Fhc.seq check
>D06399A7_A9Fh_2_BGH_rev2_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN GGNNNNNNNA CAGATGGCTG GCAACTAGAA
   61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GGAATTCTTA CTTTTTCTTT TTTGCCTGGC
  121 CGGCCTTTTT CGTGGCCGCC GGCCTTTTGT CGCCTCCCAG CTGAGACAGG TCGATCCGTG
  181 TCTCGTACAG GCCGGTGATG CTCTGGTGGA TCAGGGTGGC GTCCAGCACC TCTTTGGTGC
  241 TGGTGTACCT CTTCCGGTCG ATGGTGGTGT CAAAGTACTT GAAGGCGGCA GGGGCTCCCA
  301 GATTGGTCAG GGTAAACAGG TGGATGATAT TCTCGGCCTG CTCTCTGATG GGCTTATCCC
  361 GGTGCTTGTT GTAGGCGGAC AGCACTTTGT CCAGATTAGC GTCGGCCAGG ATCACTCTCT
  421 TGGAGAACTC GCTGATCTGC TCGATGATCT CGTCCAGGTA GTGCTTGTGC TGTTCCACAA
  481 ACAGCTGTTT CTGCTCATTA TCCTCGGGGG AGCCCTTCAG CTTCTCATAG TGGCTGGCCA
  541 GGTACAGGAA GTTCACATAT TTGGAGGGCA GGGCCAGTTC GTTTCCCTTC TGCAGTTCGC
  601 CGGCAGAGGC CAGCATTCTC TTCCGGCCGT TTTCCAGCTC GAACAGGGAG TACTTAGGCA
  661 GCTTGATGAT CAGGTCCTTT TTCACTTCTT TGTAGCCCTT GGCTTCCAGA AAGTCGATGG
  721 GANTCTTCTC GAAGCTGCTT CTTTCCATGA TGGTGATCCC CAGCAGCTCT TTCACACTCT
  781 TCAGTTTCTT GGACTTGCCC TTTTCCACTT TGGCCACCAC CAGCACAGAA TANGCCACGG
  841 NGGGGCTGTC GAANCCGCCG NACTTCTTAN GGTCCCAGTC CTTCTTTCTG GCNANCAGCN
  901 TATCGCTGTN CCNCNNNGGC ANGATAGANN CNTTNNCTNA AGCCNNNNGN NNGCNNCNNN
961 GGNCNTTTTT CNCNANNNTN NNNNTNNNGG NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]