icon below as well as clone information before placing your order.
Expression vector of guide RNA for targeting human CDCA7 exon 7.
| Catalog number | RDB17380 |
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| Resource name | CDCA7 px330 |
| Alternative name | CDCA7 exon7 pX330-U6-Chimeric_BB-CBh-hSpCas9 |
| Clone info. | Expression vector of guide RNA for targeting human cell division cycle associated 7 (CDCA7) exon 7 (5'-TCAGAAGACTATTGATACCA-3') and human codon-optimized SpCas9. |
| Vector backbone | pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid) |
| Size of vector backbone | 8.5 kb |
| Selectable markers | Amp^r (E. coli) |
| Gene/insert name | human CDCA7 dsOligo |
| Depositor|Developer | Unoki, Motoko | |
| Other clones in our bank | human CDCA7 (NCBI Gene 83879) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested. |
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| Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment  ] [Bank Transfer or Check Payment]Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word ] |
| Remarks | The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose). |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB17380 | CDCA7 px330 | DNA solution |
icon below as well as clone information before placing your order.Electronic file
Original reference
| original | Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. |
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Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB17380_A9Fhp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: SV40pA3'element reverse Region: U6 pro, gRNA (CDCA7) gRNA scaffold, CBh pro 5' Sequence file: RDB17380_A9Fha.seq
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>D06399A7_A9Fh_2_SV40pA3'element reverse_C03_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG CNNGCAACGC GGCCTTTTTA CGGTTCCTGG 61 CCTTTTGCTG GCCTTTTGCT CACATGTGAG GGCCTATTTC CCATGATTCC TTCATATTTG 121 CATATACGAT ACAAGGCTGT TAGAGAGATA ATTGGAATTA ATTTGACTGT AAACACAAAG 181 ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA 241 AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC 301 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGTGG TATCAATAGT CTTCTGAGTT 361 TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC 421 ACCGAGTCGG TGCTTTTTTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC 481 GTTTTTAGCG CGTGCGCCAA TTCTGCAGAC AAATGGCTCT AGAGGTACCC GTTACATAAC 541 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAG 601 TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC 661 ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG 721 GTAAATGGCC CGCCTGGCAT TGTGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC 781 AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTCGA NGTGAGCCCC ACGTTCTGCT 841 TCACTCTCCC CATCTCCCCC CCCCNNCCCA CCCCCAATTT TGTATTTATT TATTTTTTTA 901 ATTATTTTGT GCAGCGATGG GGGCGGGGGG GGGGGGGGGG GGNNNNNNNN NN // |
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Primer: Cas9-N_R1 Region: Cas9 5', SV40 NLS, 3xFLAG, CBh pro 3' Sequence file: RDB17380_A9Fhb.seq
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>D06399A7_A9Fh_2_Cas9-N_R1_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNGNNNNGN NNNTTGNNNT CGTCGGTGNN CACGGCCCAG CCCACAGAGT 61 TGGTGCCGAT GTCCAGGCCG ATGCTGTACT TCTTGTCGGC TGCTGGGACT CCGTGGATAC 121 CGACCTTCCG CTTCTTCTTT GGGGCCATCT TATCGTCATC GTCTTTGTAA TCAATATCAT 181 GATCCTTGTA GTCTCCGTCG TGGTCCTTAT AGTCCATGGT GGCACCGGTC CAACCTGAAA 241 AAAAGTGATT TCAGGCAGGT GCTCCAGGTA ATTAAACATT AATACCCCAC CAACCAACCA 301 TCCCTTAAAC CCTTACCTCT TGCTCAGCTA ATTACAGCCC GGAGGAGAAG GGCCGTCCCG 361 CCCGCTCACC TGTGGGAGTA ACGCGGTCAG TCAGAGCCGG GGCGGGCGGC GCGAGGNN // |
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Primer: BGH_rev2 Region: bGH p(A), nucleoplasmin NLS, Cas9 3' Sequence file: RDB17380_A9Fhc.seq
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>D06399A7_A9Fh_2_BGH_rev2_B02_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN GGNNNNNNNA CAGATGGCTG GCAACTAGAA 61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GGAATTCTTA CTTTTTCTTT TTTGCCTGGC 121 CGGCCTTTTT CGTGGCCGCC GGCCTTTTGT CGCCTCCCAG CTGAGACAGG TCGATCCGTG 181 TCTCGTACAG GCCGGTGATG CTCTGGTGGA TCAGGGTGGC GTCCAGCACC TCTTTGGTGC 241 TGGTGTACCT CTTCCGGTCG ATGGTGGTGT CAAAGTACTT GAAGGCGGCA GGGGCTCCCA 301 GATTGGTCAG GGTAAACAGG TGGATGATAT TCTCGGCCTG CTCTCTGATG GGCTTATCCC 361 GGTGCTTGTT GTAGGCGGAC AGCACTTTGT CCAGATTAGC GTCGGCCAGG ATCACTCTCT 421 TGGAGAACTC GCTGATCTGC TCGATGATCT CGTCCAGGTA GTGCTTGTGC TGTTCCACAA 481 ACAGCTGTTT CTGCTCATTA TCCTCGGGGG AGCCCTTCAG CTTCTCATAG TGGCTGGCCA 541 GGTACAGGAA GTTCACATAT TTGGAGGGCA GGGCCAGTTC GTTTCCCTTC TGCAGTTCGC 601 CGGCAGAGGC CAGCATTCTC TTCCGGCCGT TTTCCAGCTC GAACAGGGAG TACTTAGGCA 661 GCTTGATGAT CAGGTCCTTT TTCACTTCTT TGTAGCCCTT GGCTTCCAGA AAGTCGATGG 721 GANTCTTCTC GAAGCTGCTT CTTTCCATGA TGGTGATCCC CAGCAGCTCT TTCACACTCT 781 TCAGTTTCTT GGACTTGCCC TTTTCCACTT TGGCCACCAC CAGCACAGAA TANGCCACGG 841 NGGGGCTGTC GAANCCGCCG NACTTCTTAN GGTCCCAGTC CTTCTTTCTG GCNANCAGCN 901 TATCGCTGTN CCNCNNNGGC ANGATAGANN CNTTNNCTNA AGCCNNNNGN NNGCNNCNNN 961 GGNCNTTTTT CNCNANNNTN NNNNTNNNGG NNNNNNNNNN // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. |
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2021.03.11
GNP_filter3_RDBDEP_html_210308.pl