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ZBTB24 px330 (#RDB17379)

Expression vector of guide RNA for targeting human ZBTB24 exon 4.

Alternative name ZBTB24 exon4 pX330-U6-Chimeric_BB-CBh-hSpCas9
Clone info. Expression vector of guide RNA for targeting human zinc finger and BTB domain containing 24 (ZBTB24) exon 4 (5'-GTGATCAATGCGGAAAATAT-3') and human codon-optimized SpCas9.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r (E. coli)
Gene/insert name human ZBTB24 dsOligo
Depositor|Developer Unoki, Motoko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
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必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

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RDB17379 ZBTB24 px330 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The ZBTB24 px330 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17379).

Reference section:

Unoki, M., Funabiki, H., Velasco, G., Francastel, C., Sasaki, H., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17379_A9Fhp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'element reverse (Pr0108)
Region: U6 pro, gRNA (ZBTB24) gRNA scaffold, CBh pro 5'
Sequence file: RDB17379_A9Fha.seq check
>D06399A6_A9Fh_2_SV40pA3'element reverse_H02_23_ABI24.ab1
    1 NNNNNNNNNN GNNGGNNNNN TGGNANNNGC NNGCAACGCG GCCTTTTTNC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTGAGG GCCTATTTCC CATGATTCCT TCATATTTGC
  121 ATATACGATA CAAGGCTGTT AGAGAGATAA TTGGAATTAA TTTGACTGTA AACACAAAGA
  181 TATTAGTACA AAATACGTGA CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA
  241 AATTATGTTT TAAAATGGAC TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT
  301 TGGCTTTATA TATCTTGTGG AAAGGACGAA ACACCGATAT TTTCCGCATT GATCACGTTT
  361 TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA AAAAGTGGCA
  421 CCGAGTCGGT GCTTTTTTGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG
  481 TTTTTAGCGC GTGCGCCAAT TCTGCAGACA AATGGCTCTA GAGGTACCCG TTACATAACT
  541 TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAGT
  601 AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA
  661 CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG
  721 TAAATGGCCC GCCTGGCATT GTGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA
  781 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTCGAN GTGANCCCCA CGTTCTGCTT
  841 CACTCTCCCC ATCTCCCCCC CCCTCCCCAC CCCCNATTTT GTATTTATTT ATTTTTTAAT
901 TATTTTGTGC AGCGANGGGG NNNNNNNNNN
//
Primer: Cas9-N_R1 (Pr0650)
Region: Cas9 5', SV40 NLS, 3xFLAG, CBh pro 3'
Sequence file: RDB17379_A9Fhb.seq check
>D06399A6_A9Fh_2_Cas9-N_R1_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTNGTNN TCGTCGGTGN NCNCGGCCCN GCCCNCAGAG
   61 TTGGTGCCGA TGTCCAGGCC GATGCTGTAC TTCTTGTCGG CTGCTGGGAC TCCGTGGATA
  121 CCGACCTTCC GCTTCTTCTT TGGGGCCATC TTATCGTCAT CGTCTTTGTA ATCAATATCA
  181 TGATCCTTGT AGTCTCCGTC GTGGTCCTTA TAGTCCATGG TGGCACCGGT CCAACCTGAA
  241 AAAAAGTGAT TTCAGGCAGG TGCTCCAGGT AATTAAACAT TAATACCCCA CCAACCAACC
  301 ATCCCTTAAA CCCTTACCTC TTGCTCAGCT AATTACAGCC CGGAGGAGAA GGGCCGTCCC
361 GCCCGCTCAC CTGTGGGAGT AACGCGGTCA GTCAGAGCCG GGGCGGGCGG CGCGAGGNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH p(A), nucleoplasmin NLS, Cas9 3'
Sequence file: RDB17379_A9Fhc.seq check
>D06399A6_A9Fh_2_BGH_rev2_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGNNGNNGG GCNAACNACA GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GAGCTCTAGG AATTCTTACT TTTTCTTTTT TGCCTGGCCG
  121 GCCTTTTTCG TGGCCGCCGG CCTTTTGTCG CCTCCCAGCT GAGACAGGTC GATCCGTGTC
  181 TCGTACAGGC CGGTGATGCT CTGGTGGATC AGGGTGGCGT CCAGCACCTC TTTGGTGCTG
  241 GTGTACCTCT TCCGGTCGAT GGTGGTGTCA AAGTACTTGA AGGCGGCAGG GGCTCCCAGA
  301 TTGGTCAGGG TAAACAGGTG GATGATATTC TCGGCCTGCT CTCTGATGGG CTTATCCCGG
  361 TGCTTGTTGT AGGCGGACAG CACTTTGTCC AGATTAGCGT CGGCCAGGAT CACTCTCTTG
  421 GAGAACTCGC TGATCTGCTC GATGATCTCG TCCAGGTAGT GCTTGTGCTG TTCCACAAAC
  481 AGCTGTTTCT GCTCATTATC CTCGGGGGAG CCCTTCAGCT TCTCATAGTG GCTGGCCAGG
  541 TACAGGAAGT TCACATATTT GGAGGGCAGG GCCAGTTCGT TTCCCTTCTG CAGTTCGCCG
  601 GCAGAGGCCA GCATTCTCTT CCGGCCGTTT TCCAGCTCGA ACAGGGAGTA CTTAGGCAGC
  661 TTGATGATCA GGTCCTTTTT CACTTCTTTG TAGCCCTTGG CTTCCAGAAA GTCGATGGGA
  721 TTCTTCTCGA AGCTGCTTCT TTCCATGATG GTGATCCCCA GCAGCTCTTT CNCACTCTTC
  781 AGTTTCTTGG ACTTGCCCTT TTCCACTTTG GCCACCACCA GCACAGAATA NGCCACGGNN
  841 NGGCTGTCGA AGCCGCCGTA CTTCTTANGG NCCCAGNNCT TCTTTCTGGC GATCAGCTTA
  901 TCGCNNTNCC TCTTGGGCAG GATAGANTCT TTGCTGAAGC CGNCTGNCTG CNNNNCGGNC
  961 TTTTTCNCNA NATNCACTNN GGGGCANGNN CAGCNNTTNN CNGNNCNGNN NNNNAANNNN
1021 NNGNNNNNTN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]