RDB17378 - DNMT3B px330

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DNMT3B px330

Expression vector of guide RNA for targeting human DNMT3B exon 6.


Catalog number	RDB17378
Resource name	DNMT3B px330
Alternative name	DNMT3B exon6 pX330-U6-Chimeric_BB-CBh-hSpCas9
Clone info.	Expression vector of guide RNA for targeting human DNA methyltransferase 3 beta (DNMT3B) exon 6 (5'-GAGACGGCGGGCAACAGCAT-3') and human codon-optimized SpCas9.
Vector backbone	pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone	8.5 kb
Selectable markers	Amp^r (E. coli)
Gene/insert name	human DNMT3B dsOligo
Depositor\|Developer	Unoki, Motoko \|
Other clones in our bank	human DNMT3B (NCBI Gene 1789) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.

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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]

Remarks
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用非営利学術目的）をお使いください [Word]
備考	本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA（CRISPR/Cas9内包遺伝子材料専用非営利学術目的）をご参照ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB17378	DNMT3B px330	DNA solution

Ordering Information [in Japanese] [in English]
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References and tips

Electronic file

Original reference

original	Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17378_A9Fhp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'element reverse Region: U6 pro, gRNA (DNMT3B) gRNA scaffold, CBh pro 5' Sequence file: RDB17378_A9Fha.seq
>D06399A5_A9Fh_2_SV40pA3'element reverse_H01_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGGNNNNNN CNNGCAACGC GGCCTTTTTA CGGTTCCTGG 61 CCTTTTGCTG GCCTTTTGCT CACATGTGAG GGCCTATTTC CCATGATTCC TTCATATTTG 121 CATATACGAT ACAAGGCTGT TAGAGAGATA ATTGGAATTA ATTTGACTGT AAACACAAAG 181 ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA 241 AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC 301 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGATG CTGTTGCCCG CCGTCTCGTT 361 TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC 421 ACCGAGTCGG TGCTTTTTTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC 481 GTTTTTAGCG CGTGCGCCAA TTCTGCAGAC AAATGGCTCT AGAGGTACCC GTTACATAAC 541 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAG 601 TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC 661 ACTTGGCAGT ACATCAAGTG TATCATATGC CNAGTACGCC CCCTATTGAC GTCAATGACG 721 GTAAATGNNC CGCCTGGCAT TGTGCCCAGT ACATGACCTT ATGNNNCTNT CCTACTTGGC 781 AGTACATCTA CGTATTAGTC ATCNCTATTA CCATGGTCNA NGTGAGCCCC ACGTTCTGCT 841 TCACTCTCCC NNTCNCCCCC CCCNTCCCNN CCCCCNATTT TGNATTTATT TATTTTTTTA 901 ATTATTTTGT GCNGCGANGG GNGNNNNNNN NNN //
Primer: Cas9-N_R1 Region: Cas9 5', SV40 NLS, 3xFLAG, CBh pro 3' Sequence file: RDB17378_A9Fhb.seq
>D06399A5_A9Fh_2_Cas9-N_R1_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNTGNNCTC GTCGGTGNNC ACGGCCCNGC CCACAGAGTT 61 GGTGCCGATG TCCAGGCCGA TGCTGTACTT CTTGTCGGCT GCTGGGACTC CGTGGATACC 121 GACCTTCCGC TTCTTCTTTG GGGCCATCTT ATCGTCATCG TCTTTGTAAT CAATATCATG 181 ATCCTTGTAG TCTCCGTCGT GGTCCTTATA GTCCATGGTG GCACCGGTCC AACCTGAAAA 241 AAAGTGATTT CAGGCAGGTG CTCCAGGTAA TTAAACATTA ATACCCCACC AACCAACCAT 301 CCCTTAAACC CTTACCTCTT GCTCAGCTAA TTACAGCCCG GAGGAGAAGG GCCGTCCCGC 361 CCGCTCACCT GTGGGAGTAA CGCGGTCAGT CAGAGCCGGG GCGGGCGGCG CGAGGCN //
Primer: BGH_rev2 Region: bGH p(A), nucleoplasmin NLS, Cas9 3' Sequence file: RDB17378_A9Fhc.seq
>D06399A5_A9Fh_2_BGH_rev2_G02_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GGCNNACNAC AGATGGCTGG CAACTAGAAG 61 GCACAGTCGA GGCTGATCAG CGAGCTCTAG GAATTCTTAC TTTTTCTTTT TTGCCTGGCC 121 GGCCTTTTTC GTGGCCGCCG GCCTTTTGTC GCCTCCCAGC TGAGACAGGT CGATCCGTGT 181 CTCGTACAGG CCGGTGATGC TCTGGTGGAT CAGGGTGGCG TCCAGCACCT CTTTGGTGCT 241 GGTGTACCTC TTCCGGTCGA TGGTGGTGTC AAAGTACTTG AAGGCGGCAG GGGCTCCCAG 301 ATTGGTCAGG GTAAACAGGT GGATGATATT CTCGGCCTGC TCTCTGATGG GCTTATCCCG 361 GTGCTTGTTG TAGGCGGACA GCACTTTGTC CAGATTAGCG TCGGCCAGGA TCACTCTCTT 421 GGAGAACTCG CTGATCTGCT CGATGATCTC GTCCAGGTAG TGCTTGTGCT GTTCCACAAA 481 CAGCTGTTTC TGCTCATTAT CCTCGGGGGA GCCCTTCAGC TTCTCATAGT GGCTGGCCAG 541 GTACAGGAAG TTCACATATT TGGAGGGCAG GGCCAGTTCG TTTCCCTTCT GCAGTTCGCC 601 GGCAGAGGCC AGCATTCTCT TCCGGCCGTT TTCCAGCTCG AACAGGGAGT ACTTAGGCAG 661 CTTGATGATC AGGTCCTTTT TCACTTCTTT GTAGCCCTTG GCTTCCAGAA AGTCGATGGG 721 ANTCTTCTCG AAGCTGCTTC TTTCCATGAT GGTGATCCCC AGCAGCTCTT TCACACTCTT 781 CAGTTTCTTG GANTTGCCCT TTTCCACTTT GGCCACCACC AGCACAGAAT ANGCCACGGN 841 GGGGCTGTCG AANCCGCCNT ACTTCTTANG GTCCCAGTCC TTCTTTNTGG CGATCAGCTT 901 ATCNCTGTTN NNCNNGGGNA NGANAGANTC TTTGCTGAAN CCGNCNGNCT GCNNNNNGGN 961 CTTTTTCNCN ATATTCNNNN GGGGNANGNN CNNNNNNTNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408.

2021.03.11

GNP_filter3_RDBDEP_html_210308.pl