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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

DNMT3B px330 (#RDB17378)

Expression vector of guide RNA for targeting human DNMT3B exon 6.

Alternative name DNMT3B exon6 pX330-U6-Chimeric_BB-CBh-hSpCas9
Clone info. Expression vector of guide RNA for targeting human DNA methyltransferase 3 beta (DNMT3B) exon 6 (5'-GAGACGGCGGGCAACAGCAT-3') and human codon-optimized SpCas9.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r (E. coli)
Gene/insert name human DNMT3B dsOligo
Depositor|Developer Unoki, Motoko |
 

Distribution information

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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
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必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

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RDB17378 DNMT3B px330 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The DNMT3B px330 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17378).

Reference section:

Unoki, M., Funabiki, H., Velasco, G., Francastel, C., Sasaki, H., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17378_A9Fhp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'element reverse (Pr0108)
Region: U6 pro, gRNA (DNMT3B) gRNA scaffold, CBh pro 5'
Sequence file: RDB17378_A9Fha.seq check
>D06399A5_A9Fh_2_SV40pA3'element reverse_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGNNNNNN CNNGCAACGC GGCCTTTTTA CGGTTCCTGG
   61 CCTTTTGCTG GCCTTTTGCT CACATGTGAG GGCCTATTTC CCATGATTCC TTCATATTTG
  121 CATATACGAT ACAAGGCTGT TAGAGAGATA ATTGGAATTA ATTTGACTGT AAACACAAAG
  181 ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA
  241 AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC
  301 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGATG CTGTTGCCCG CCGTCTCGTT
  361 TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC
  421 ACCGAGTCGG TGCTTTTTTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC
  481 GTTTTTAGCG CGTGCGCCAA TTCTGCAGAC AAATGGCTCT AGAGGTACCC GTTACATAAC
  541 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAG
  601 TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC
  661 ACTTGGCAGT ACATCAAGTG TATCATATGC CNAGTACGCC CCCTATTGAC GTCAATGACG
  721 GTAAATGNNC CGCCTGGCAT TGTGCCCAGT ACATGACCTT ATGNNNCTNT CCTACTTGGC
  781 AGTACATCTA CGTATTAGTC ATCNCTATTA CCATGGTCNA NGTGAGCCCC ACGTTCTGCT
  841 TCACTCTCCC NNTCNCCCCC CCCNTCCCNN CCCCCNATTT TGNATTTATT TATTTTTTTA
901 ATTATTTTGT GCNGCGANGG GNGNNNNNNN NNN
//
Primer: Cas9-N_R1 (Pr0650)
Region: Cas9 5', SV40 NLS, 3xFLAG, CBh pro 3'
Sequence file: RDB17378_A9Fhb.seq check
>D06399A5_A9Fh_2_Cas9-N_R1_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTGNNCTC GTCGGTGNNC ACGGCCCNGC CCACAGAGTT
   61 GGTGCCGATG TCCAGGCCGA TGCTGTACTT CTTGTCGGCT GCTGGGACTC CGTGGATACC
  121 GACCTTCCGC TTCTTCTTTG GGGCCATCTT ATCGTCATCG TCTTTGTAAT CAATATCATG
  181 ATCCTTGTAG TCTCCGTCGT GGTCCTTATA GTCCATGGTG GCACCGGTCC AACCTGAAAA
  241 AAAGTGATTT CAGGCAGGTG CTCCAGGTAA TTAAACATTA ATACCCCACC AACCAACCAT
  301 CCCTTAAACC CTTACCTCTT GCTCAGCTAA TTACAGCCCG GAGGAGAAGG GCCGTCCCGC
361 CCGCTCACCT GTGGGAGTAA CGCGGTCAGT CAGAGCCGGG GCGGGCGGCG CGAGGCN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH p(A), nucleoplasmin NLS, Cas9 3'
Sequence file: RDB17378_A9Fhc.seq check
>D06399A5_A9Fh_2_BGH_rev2_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GGCNNACNAC AGATGGCTGG CAACTAGAAG
   61 GCACAGTCGA GGCTGATCAG CGAGCTCTAG GAATTCTTAC TTTTTCTTTT TTGCCTGGCC
  121 GGCCTTTTTC GTGGCCGCCG GCCTTTTGTC GCCTCCCAGC TGAGACAGGT CGATCCGTGT
  181 CTCGTACAGG CCGGTGATGC TCTGGTGGAT CAGGGTGGCG TCCAGCACCT CTTTGGTGCT
  241 GGTGTACCTC TTCCGGTCGA TGGTGGTGTC AAAGTACTTG AAGGCGGCAG GGGCTCCCAG
  301 ATTGGTCAGG GTAAACAGGT GGATGATATT CTCGGCCTGC TCTCTGATGG GCTTATCCCG
  361 GTGCTTGTTG TAGGCGGACA GCACTTTGTC CAGATTAGCG TCGGCCAGGA TCACTCTCTT
  421 GGAGAACTCG CTGATCTGCT CGATGATCTC GTCCAGGTAG TGCTTGTGCT GTTCCACAAA
  481 CAGCTGTTTC TGCTCATTAT CCTCGGGGGA GCCCTTCAGC TTCTCATAGT GGCTGGCCAG
  541 GTACAGGAAG TTCACATATT TGGAGGGCAG GGCCAGTTCG TTTCCCTTCT GCAGTTCGCC
  601 GGCAGAGGCC AGCATTCTCT TCCGGCCGTT TTCCAGCTCG AACAGGGAGT ACTTAGGCAG
  661 CTTGATGATC AGGTCCTTTT TCACTTCTTT GTAGCCCTTG GCTTCCAGAA AGTCGATGGG
  721 ANTCTTCTCG AAGCTGCTTC TTTCCATGAT GGTGATCCCC AGCAGCTCTT TCACACTCTT
  781 CAGTTTCTTG GANTTGCCCT TTTCCACTTT GGCCACCACC AGCACAGAAT ANGCCACGGN
  841 GGGGCTGTCG AANCCGCCNT ACTTCTTANG GTCCCAGTCC TTCTTTNTGG CGATCAGCTT
  901 ATCNCTGTTN NNCNNGGGNA NGANAGANTC TTTGCTGAAN CCGNCNGNCT GCNNNNNGGN
961 CTTTTTCNCN ATATTCNNNN GGGGNANGNN CNNNNNNTNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]