Please review the QC test results indicated by icon below as well as clone information before placing your order.

p3XFLAG-CDCA7 R274C

Expression vector of human CDCA7 mutant, R274C.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17375_A9Fhp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Region: CMV pro, 3xFLAG, insert 5' Sequence file: RDB17375_A9Fha.seq
>D06399A2_A9Fh_2_CMV_Forward_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNNGCNNTG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT    61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG AATTAACCAT GGACTACAAA GACCATGACG   121 GTGATTATAA AGATCATGAC ATCGATTACA AGGATGACGA TGACAAGCTT GCGGCCGCGA   181 ATTCATCGAT AGATCTGATA TCGGTACCAA TGGACGCTCG CCGCGTGCCG CAGAAAGATC   241 TCAGAGTAAA GAAGAACTTA AAGAAATTCA GATATGTGAA GTTGATTTCC ATGGAAACCT   301 CGTCATCCTC TGATGACAGT TGTGACAGCT TTGCTTCTGA TAATTTTGCA AACACGAGGC   361 TGCAGTCAGT TCGGGAAGGC TGTAGGACCC GCAGCCAGTG CAGGCACTCT GGACCTCTCA   421 GGGTGGCGAT GAAGTTTCCA GCGCGGAGTA CCAGGGGAGC AACCAACAAA AAAGCAGAGT   481 CCCGCCAGCC CTCAGAGAAT TCTGTGACTG ATTCCAACTC CGATTCAGAA GATGAAAGTG   541 GAATGAATTT TTTGGAGAAA AGGGCTTTAA ATATAAAGCA AAACAAAGCA ATGCTTGCAA   601 AACTCATGTC TGAATTAGAA AGCTTCCCTG GCTCGTTCCG TGGAAGACAT CCCCTCCCAG   661 GCTCCGACTC ACAATCAAGG AGACCGCGAA GGCGTACATT CCCGGGTGTT GCTTCCAGGA   721 GAAACCCTGA ACGGAGAGCT CGTCCTCTTA CCAGGTCAAG GTCCCGGATC CTCGGGTCCC   781 TTGACGCTCT ACCCATGGAG GAGGANGANG AAGANGATAA GTACATGTTG GTGANAAAGA   841 NGAAGANCGT GGATGGCTAC NTGAATGAAG ATGANCTGCC CAGAAGCCGN CGCTCCNGAT   901 CATCCGTGAC CCTTNCGCAT ATAATTCGCC CANNGNAAGA AATTACAGAG GANGANTTNG 961 ANAACGNNTG CNNCNNNTNT CGANANNNGA NNTATNNNNN NNNNN //
Primer: hGHpolyA-R Region: hGHp(A), insert 3' Sequence file: RDB17375_A9Fhb.seq
>D06399A2_A9Fh_2_hGHpolyA-R_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNANNNN NNAAGGNNAC CTAGTCAGAC AAAATGATGC    61 AACTTAATTT TATTAGGACA AGGCTGGTGG GCACTGGAGT GGCAACTTCC AGGGCCAGGA   121 GAGGCACTGG GGAGGGGTCA CAGGGATGCC ACCCGGGATC CTCTAGATTA TGCTTGCATT   181 TCAAATTCCT GTTTCAGGCT TTTCAAGTAG GCATGCACAT TCCCAAAGCC ATGATATTTG   241 GCTAAATACA CAAGGACCCC AGTCGCACAC CGTCCATCTC GCTGCCGGCA GAAACTGCAG   301 TTGCAGATTC CTCGACAAGG CGGGCAATGC CAGTTCGGAT CCAGCAGAGC ATCCCTGACC   361 TCTTCACCAT AACGGTTTCG AAGGCAGGGG CCACAGAACT GGCCTCGAAC GCCCCAGCAG   421 TCTGGGTTTC TGCAGTTTGT TTTGGTATCA ATAGTCTTCT GACAGCATTG ATGACAAGTA   481 GAGCCCAGTG AACGGTTATA TATCTTCTCT CGAGAATTGC TGCAGACGTT CTCCAACTCC   541 TCCTCTGTAA TTTCTTCCAC TGGGCGAATT ATATGCGGAA GGGTCACGGA TGATCTGGAG   601 CGACGGCTTC TGGGCAGGTC ATCTTCATTC ATGTAGCCAT CCACGGTCTT CCTCTTTCTC   661 ACCAACATGT ACTTATCCTC TTCCTCCTCC TCCTCCATGG GTAGAGCGTC AAGGGACCCG   721 AGGATCCGGG ACCTTGANCT GNTAAGAGGA CGAGCTCTCC GTTCANGGTT TCTCCTGGAA   781 GCAACACCCG GGAATGTACG CCTTCGCGGT CTCCTTGATT GTGANTCNGA NCCTGGGANG   841 GGANGTCTTC CACNGAACGA NCCNNGGAAG CTTTCTANTT CNNANATGAN TTTTGCNAGC   901 NTTGCTTTGT TTTGCTTTAT ATTTNAAGCC CTTTTCTCCN AAAAANNTNN TTNNNNTTTN 961 NTCTTCTGAN NCNGNANNNN GNNANNNNNC ACAGNNNTNN NNNGANGNNN NNNNNNN //
Primer: SV40pro_F Region: NeoR/KanR Sequence file: RDB17375_A9Fhc.seq
>D06399A2_A9Fh_2_SV40pro_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNAGGANGCT TTTTTGGANG ANNNNNNNNN    61 GCTTGGGATC TGATCNAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT   121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC   181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC   241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC   301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG   361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC   421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG   481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC   541 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC   601 CAGCCGAACT GTTCGCCNGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA   661 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCN   721 TCGACTGTGG CCGGCTGGGT GTGGCNGANC GCTATCAGGN NNNNNCGTTG GCTACNCGTG   781 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCNTGCTT TACNNNATCG   841 CCGCTCCCNA NTCNCNNCGC ATCNCCTTCT ATCGCNNTNT TGANNANNNC NTCTGANCGN   901 GACTCTGGGG TTNNAANNGA CNNNNCANNN NACGCCCNNN NCNNGNCNTC NCNAGANTTN 961 CNATNCCNNN GNNNNNNTCN ATNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl