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p3XFLAG-CDCA7 R274C (#RDB17375)

Expression vector of human CDCA7 mutant, R274C.

Alternative name p3XFLAG-CMV10-CDCA7 R274C
Clone info. Expression vector of human cell division cycle associated 7 (CDCA7) mutant, R274C, tagged with 3 x FLAG at N-terminus (cloning sites: KpnI, XbaI). This mutation corresponds to an amino acid substitution identified in an ICF3 patient and is located in the 4-CXXC zinc finger domain. Structure, see Fig. S1 of Unoki, M. et al., J. Clin. Invest., 129 (1): 78-92, 2019.
Comment Expression was confirmed by the depositor with western blottingand RT-PCR.
Vector backbone p3xFLAG-CMV-10 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human CDCA7 cDNA
Depositor|Developer Unoki, Motoko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。

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RDB17375 p3XFLAG-CDCA7 R274C DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The p3XFLAG-CDCA7 R274C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17375).

Reference section:

Unoki, M., Funabiki, H., Velasco, G., Francastel, C., Sasaki, H., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17375_A9Fhp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Region: CMV pro, 3xFLAG, insert 5'
Sequence file: RDB17375_A9Fha.seq check
>D06399A2_A9Fh_2_CMV_Forward_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNGCNNTG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG AATTAACCAT GGACTACAAA GACCATGACG
  121 GTGATTATAA AGATCATGAC ATCGATTACA AGGATGACGA TGACAAGCTT GCGGCCGCGA
  181 ATTCATCGAT AGATCTGATA TCGGTACCAA TGGACGCTCG CCGCGTGCCG CAGAAAGATC
  241 TCAGAGTAAA GAAGAACTTA AAGAAATTCA GATATGTGAA GTTGATTTCC ATGGAAACCT
  301 CGTCATCCTC TGATGACAGT TGTGACAGCT TTGCTTCTGA TAATTTTGCA AACACGAGGC
  361 TGCAGTCAGT TCGGGAAGGC TGTAGGACCC GCAGCCAGTG CAGGCACTCT GGACCTCTCA
  421 GGGTGGCGAT GAAGTTTCCA GCGCGGAGTA CCAGGGGAGC AACCAACAAA AAAGCAGAGT
  481 CCCGCCAGCC CTCAGAGAAT TCTGTGACTG ATTCCAACTC CGATTCAGAA GATGAAAGTG
  541 GAATGAATTT TTTGGAGAAA AGGGCTTTAA ATATAAAGCA AAACAAAGCA ATGCTTGCAA
  601 AACTCATGTC TGAATTAGAA AGCTTCCCTG GCTCGTTCCG TGGAAGACAT CCCCTCCCAG
  661 GCTCCGACTC ACAATCAAGG AGACCGCGAA GGCGTACATT CCCGGGTGTT GCTTCCAGGA
  721 GAAACCCTGA ACGGAGAGCT CGTCCTCTTA CCAGGTCAAG GTCCCGGATC CTCGGGTCCC
  781 TTGACGCTCT ACCCATGGAG GAGGANGANG AAGANGATAA GTACATGTTG GTGANAAAGA
  841 NGAAGANCGT GGATGGCTAC NTGAATGAAG ATGANCTGCC CAGAAGCCGN CGCTCCNGAT
  901 CATCCGTGAC CCTTNCGCAT ATAATTCGCC CANNGNAAGA AATTACAGAG GANGANTTNG
961 ANAACGNNTG CNNCNNNTNT CGANANNNGA NNTATNNNNN NNNNN
//
Primer: hGHpolyA-R ()
Region: hGHp(A), insert 3'
Sequence file: RDB17375_A9Fhb.seq check
>D06399A2_A9Fh_2_hGHpolyA-R_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNANNNN NNAAGGNNAC CTAGTCAGAC AAAATGATGC
   61 AACTTAATTT TATTAGGACA AGGCTGGTGG GCACTGGAGT GGCAACTTCC AGGGCCAGGA
  121 GAGGCACTGG GGAGGGGTCA CAGGGATGCC ACCCGGGATC CTCTAGATTA TGCTTGCATT
  181 TCAAATTCCT GTTTCAGGCT TTTCAAGTAG GCATGCACAT TCCCAAAGCC ATGATATTTG
  241 GCTAAATACA CAAGGACCCC AGTCGCACAC CGTCCATCTC GCTGCCGGCA GAAACTGCAG
  301 TTGCAGATTC CTCGACAAGG CGGGCAATGC CAGTTCGGAT CCAGCAGAGC ATCCCTGACC
  361 TCTTCACCAT AACGGTTTCG AAGGCAGGGG CCACAGAACT GGCCTCGAAC GCCCCAGCAG
  421 TCTGGGTTTC TGCAGTTTGT TTTGGTATCA ATAGTCTTCT GACAGCATTG ATGACAAGTA
  481 GAGCCCAGTG AACGGTTATA TATCTTCTCT CGAGAATTGC TGCAGACGTT CTCCAACTCC
  541 TCCTCTGTAA TTTCTTCCAC TGGGCGAATT ATATGCGGAA GGGTCACGGA TGATCTGGAG
  601 CGACGGCTTC TGGGCAGGTC ATCTTCATTC ATGTAGCCAT CCACGGTCTT CCTCTTTCTC
  661 ACCAACATGT ACTTATCCTC TTCCTCCTCC TCCTCCATGG GTAGAGCGTC AAGGGACCCG
  721 AGGATCCGGG ACCTTGANCT GNTAAGAGGA CGAGCTCTCC GTTCANGGTT TCTCCTGGAA
  781 GCAACACCCG GGAATGTACG CCTTCGCGGT CTCCTTGATT GTGANTCNGA NCCTGGGANG
  841 GGANGTCTTC CACNGAACGA NCCNNGGAAG CTTTCTANTT CNNANATGAN TTTTGCNAGC
  901 NTTGCTTTGT TTTGCTTTAT ATTTNAAGCC CTTTTCTCCN AAAAANNTNN TTNNNNTTTN
961 NTCTTCTGAN NCNGNANNNN GNNANNNNNC ACAGNNNTNN NNNGANGNNN NNNNNNN
//
Primer: SV40pro_F ()
Region: NeoR/KanR
Sequence file: RDB17375_A9Fhc.seq check
>D06399A2_A9Fh_2_SV40pro_F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNAGGANGCT TTTTTGGANG ANNNNNNNNN
   61 GCTTGGGATC TGATCNAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT
  121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
  181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
  241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC
  301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
  361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
  421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
  481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC
  541 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC
  601 CAGCCGAACT GTTCGCCNGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA
  661 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCN
  721 TCGACTGTGG CCGGCTGGGT GTGGCNGANC GCTATCAGGN NNNNNCGTTG GCTACNCGTG
  781 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCNTGCTT TACNNNATCG
  841 CCGCTCCCNA NTCNCNNCGC ATCNCCTTCT ATCGCNNTNT TGANNANNNC NTCTGANCGN
  901 GACTCTGGGG TTNNAANNGA CNNNNCANNN NACGCCCNNN NCNNGNCNTC NCNAGANTTN
961 CNATNCCNNN GNNNNNNTCN ATNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]