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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

p3XFLAG-CDCA7 WT (#RDB17374)

Expression vector of human CDCA7 wild-type.

Alternative name p3XFLAG-CMV10-CDCA7 WT
Clone info. Expression vector of human cell division cycle associated 7 (CDCA7) wild-type, tagged with 3 x FLAG at N-terminus. Structure, see Fig. S1 of Unoki, M. et al., J. Clin. Invest., 129 (1): 78-92, 2019.
Comment Expression was confirmed by the depositor with western blottingand RT-PCR.
Vector backbone p3xFLAG-CMV-10 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human CDCA7 cDNA
Depositor|Developer Unoki, Motoko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。

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RDB17374 p3XFLAG-CDCA7 WT DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The p3XFLAG-CDCA7 WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17374).

Reference section:

Unoki, M., Funabiki, H., Velasco, G., Francastel, C., Sasaki, H., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17374_A9Fhp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Region: CMV pro, 3xFLAG, insert 5'
Sequence file: RDB17374_A9Fha.seq check
>D06399A1_A9Fh_3_CMV_Forward_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCN NNGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGAATTAAC CATGGACTAC AAAGACCATG
  121 ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGACAAG CTTGCGGCCG
  181 CGAATTCATC GATAGATCTG ATATCGGTAC CAATGGACGC TCGCCGCGTG CCGCAGAAAG
  241 ATCTCAGAGT AAAGAAGAAC TTAAAGAAAT TCAGATATGT GAAGTTGATT TCCATGGAAA
  301 CCTCGTCATC CTCTGATGAC AGTTGTGACA GCTTTGCTTC TGATAATTTT GCAAACACGA
  361 GGCTGCAGTC AGTTCGGGAA GGCTGTAGGA CCCGCAGCCA GTGCAGGCAC TCTGGACCTC
  421 TCAGGGTGGC GATGAAGTTT CCAGCGCGGA GTACCAGGGG AGCAACCAAC AAAAAAGCAG
  481 AGTCCCGCCA GCCCTCAGAG AATTCTGTGA CTGATTCCAA CTCCGATTCA GAAGATGAAA
  541 GTGGAATGAA TTTTTTGGAG AAAAGGGCTT TAAATATAAA GCAAAACAAA GCAATGCTTG
  601 CAAAACTCAT GTCTGAATTA GAAAGCTTCC CTGGCTCGTT CCGTGGAAGA CATCCCCTCC
  661 CAGGCTCCGA CTCACAATCA AGGAGACCGC GAAGGCGTAC ATTCCCGGGT GTTGCTTCCA
  721 NGAGAAACCC TGAACGGAGA GCTCGTCCTC TTACCANGTC AAGGTCNCGG ATCCTCGGGT
  781 CCNTTGACGC TCTACCCNTG GAGGANGANG ANGAAGAGGA NAAGTACNTG TTGGTGAGAA
  841 AGANGAAGAN NGTGGNTGGN TACNTGAATG AANATGACCT GNCCNNAAGC CNNCGCTCCA
901 NATCATCCGN NANNNNTTNN NNNTNTNNNT NNNNNNNNNN
//
Primer: hGHpolyA-R ()
Region: hGHp(A), insert 3'
Sequence file: RDB17374_A9Fhb.seq check
>D06399A1_A9Fh_3_hGHpolyA-R_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNAGGA NACCTAGTCN GACAAAATGA
   61 TGCAACTTAA TTTTATTAGG ACAAGGCTGG TGGGCACTGG AGTGGCAACT TCCAGGGCCA
  121 GGAGAGGCAC TGGGGAGGGG TCACAGGGAT GCCACCCGGG ATCCTCTAGA TTATGCTTGC
  181 ATTTCAAATT CCTGTTTCAG GCTTTTCAAG TAGGCATGCA CATTCCCAAA GCCATGATAT
  241 TTGGCTAAAT ACACAAGGAC CCCAGTCGCA CACCGTCCAT CTCGCTGCCG GCAGAAACTG
  301 CAGTTGCAGA TTCCTCGACA AGGCGGGCAA TGCCAGTTCG GATCCAGCAG AGCATCCCTG
  361 ACCTCTTCAC CATAACGGTT TCGAAGGCAG GGGCCACAGA ACTGGCCTCG AACGCCCCAG
  421 CAGTCTGGGT TTCTGCAGTT TGTTTTGGTA TCAATAGTCT TCTGACGGCA TTGATGACAA
  481 GTAGAGCCCA GTGAACGGTT ATATATCTTC TCTCGAGAAT TGCTGCAGAC GTTCTCCAAC
  541 TCCTCCTCTG TAATTTCTTC CACTGGGCGA ATTATATGCG GAAGGGTCAC GGATGATCTG
  601 GAGCGACGGC TTCTGGGCAG GTCATCTTCA TTCATGTAGC CNTCCACGGT CTTCCTCTTT
  661 CTCACCAACA TGTACTTATC CTCTTCCTCC TCCTCCTCCA TGGGTAGAGC GTCAAGGGAC
  721 CCGAGGATCC GGGACCTTGA CCTGGNAAGA GGACGAGCTC TCCGTTCAGG GTTTCTCCTG
  781 NAAGCAACAC CCGGNNATGT ACGCCTTCNN GGNCTCNTNG ATTGNGAGTC GGANCCTNNN
  841 ANGGGANGTC TTCCACGGAA CGANCCNGGG AANCTTTCTA ANTNNANANA TGANTTTTGN
  901 NANCATNGCT TTGTTTTGCT TTANATTTAA AGCCCNTTTN NCCAAAAAAT NNATTNNNNT
  961 TTNNTNTTCT GAATNNGANT TGGNATCNNN CACNNNNNNN NCNNANGNNT GNNGGNNNNN
1021 NGCNTTTTTT NNNNGNNNNN NNNCNNNNNN NNNN
//
Primer: SV40pro_F ()
Region: NeoR/KanR
Sequence file: RDB17374_A9Fhc.seq check
>D06399A1_A9Fh_3_SV40pro_F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGNNNGANG NTTTTTTGGA GGANNTGATC
   61 NGCTTGGGAN CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA
  121 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA
  181 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT
  241 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG
  301 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA
  361 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC
  421 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT
  481 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT
  541 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG
  601 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG
  661 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC
  721 NTCGACTGTG GNCGGCTGGG TGTGGCGGAN CNCTATCANG ACATAGCGTT GGCTACCCGN
  781 GATATTGCTG AAGAGCTTGN CGGCGANNGG GCTGANCGCT TCCTCNTGCT TTACNNNATC
  841 NNCGCTCCCN ANTCNNNNNG NATCNNCTTC TATCNNNTNC TTGANNANNN CNTCTGANCG
  901 GGACTCTGGG GGNTCNAAAN TGACCGANNN NNCGACGNNN NNNNCNNNNN NTNNNNNNNN
961 TNNNANNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]