RDB17331 - pCAG-EGFP-NpF2164g5

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pCAG-EGFP-NpF2164g5_BV4

Expression vector of engineered molecules biliverdin (BV)-binding molecules (NpF2164g5_BV4).

Catalog number	RDB17331
Resource name	pCAG-EGFP-NpF2164g5_BV4
Alternative name	GFP-NpF2164g5_BV4 in pcDNA3
Clone info.	Expression vector of engineered molecules biliverdin (BV)-binding molecules (NpF2164g5_BV4), CAG promoter, tagged with GFP at N-terminus. NpF2164g5_BV4, can function as a near-infrared (NIR) fluorescent probe in vivo, see Fig. 4 of Fushimi, K. et al., Proc. Natl. Acad. Sci. U S A, 116 (17): 8301-8309, 2019. This molecule could be a platform for the further development of brighter NIR fluorescent probes. The GFP-NpF2164g5_BV4 was cloned into HindIII and XhoI sites of a pcDNA3 (Invitrogen) in which the CMV promoter was replaced with CAG promoter. Expression was confirmed by the depositor with fluorescence imaging.
Vector backbone	ocDNA3.1 (modified) (plasmid)
Selectable markers	E. coli, Ampicillin; mammalian cell, Neomycin
Gene/insert name	Nostoc punctiforme npf2164g5 cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Narikawa, Rei \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fushimi, K et al., Proc. Natl. Acad. Sci. USA, 116(17), 8301-8309, 2019)

Ordering Please visit Information of Request for Distribution.[link]
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]

Remarks
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fushimi, K et al., Proc. Natl. Acad. Sci. USA, 116(17), 8301-8309, 2019)
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fushimi, K et al., Proc. Natl. Acad. Sci. USA, 116(17), 8301-8309, 2019)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB17331	pCAG-EGFP-NpF2164g5_BV4	Under QC test. Please contact us.	DNA solution	Please contact us.

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original	Fushimi, K., Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin. Proc. Natl. Acad. Sci. U.S.A. 116 (17): 8301-8309 (2019). PMID 30948637.

Further references such as user reports and related articles (go to bottom)

Featured content	Fluorescent protein resources (English text)
Featured content	Fluorescent protein resources (Japanese text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Fushimi, K., Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin. Proc. Natl. Acad. Sci. U.S.A. 116 (17): 8301-8309 (2019). PMID 30948637.

2020.10.02

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