Expression vector of engineered molecules biliverdin (BV)-binding molecules (NpF2164g5_BV4).
Alternative name | GFP-NpF2164g5_BV4 in pcDNA3 |
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Clone info. | Expression vector of engineered molecules biliverdin (BV)-binding molecules (NpF2164g5_BV4), CAG promoter, tagged with GFP at N-terminus. NpF2164g5_BV4, can function as a near-infrared (NIR) fluorescent probe in vivo, see Fig. 4 of Fushimi, K. et al., Proc. Natl. Acad. Sci. U S A, 116 (17): 8301-8309, 2019. This molecule could be a platform for the further development of brighter NIR fluorescent probes. |
Comment | The GFP-NpF2164g5_BV4 was cloned into HindIII and XhoI sites of a pcDNA3 (Invitrogen) in which the CMV promoter was replaced with CAG promoter. Expression was confirmed by the depositor with fluorescence imaging. |
Vector backbone | ocDNA3.1 (modified) (plasmid) |
Selectable markers | E. coli, Ampicillin; mammalian cell, Neomycin |
Gene/insert name | Nostoc punctiforme npf2164g5 cDNA Aequorea victoria GFP cDNA |
Depositor|Developer | Narikawa, Rei | |
Sequence (full) ![]() |
RDB17331zzk01.seq provided by the depositor |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fushimi, K et al., Proc. Natl. Acad. Sci. USA, 116(17), 8301-8309, 2019) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fushimi, K et al., Proc. Natl. Acad. Sci. USA, 116(17), 8301-8309, 2019)。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB17331 | pCAG-EGFP-NpF2164g5_BV4 | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pCAG-EGFP-NpF2164g5_BV4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17331). |
Reference section:
Fushimi, K., Miyazaki, T., Kuwasaki, Y., Nakajima, T., Yamamoto, T., Suzuki, K., Ueda, Y., Miyake, K., Takeda, Y., Choi, J.H., Kawagishi, H., Park, E.Y., Ikeuchi, M., Sato, M., Narikawa, R., Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin. Proc. Natl. Acad. Sci. U.S.A. 116 (17): 8301-8309 (2019). PMID 30948637. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Fushimi, K., Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin. Proc. Natl. Acad. Sci. U.S.A. 116 (17): 8301-8309 (2019). PMID 30948637. [link to RRC of NBRP] |
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