Resource data sheet
Prev.

pCAG-EGFP-NpF2164g5_BV4

Expression vector of engineered molecules biliverdin (BV)-binding molecules (NpF2164g5_BV4).

Catalog number RDB17331
Resource name pCAG-EGFP-NpF2164g5_BV4
Alternative name GFP-NpF2164g5_BV4 in pcDNA3
Clone info. Expression vector of engineered molecules biliverdin (BV)-binding molecules (NpF2164g5_BV4), CAG promoter, tagged with GFP at N-terminus.
NpF2164g5_BV4, can function as a near-infrared (NIR) fluorescent probe in vivo, see Fig. 4 of Fushimi, K. et al., Proc. Natl. Acad. Sci. U S A, 116 (17): 8301-8309, 2019. This molecule could be a platform for the further development of brighter NIR fluorescent probes.
The GFP-NpF2164g5_BV4 was cloned into HindIII and XhoI sites of a pcDNA3 (Invitrogen) in which the CMV promoter was replaced with CAG promoter. Expression was confirmed by the depositor with fluorescence imaging.
Vector backbone ocDNA3.1 (modified) (plasmid)
Selectable markers E. coli, Ampicillin; mammalian cell, Neomycin
Gene/insert name Nostoc punctiforme npf2164g5 cDNA Aequorea victoria GFP cDNA
Depositor Narikawa, Rei |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fushimi, K et al., Proc. Natl. Acad. Sci. USA, 116(17), 8301-8309, 2019)
Ordering Please visit Information of Request for Distribution. 
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17331 pCAG-EGFP-NpF2164g5_BV4 Under QC test. Please contact us. DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Fushimi, K., Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin. Proc. Natl. Acad. Sci. U.S.A. 116 (17): 8301-8309 (2019). PMID 30948637.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

Original, user report and related articles

original Fushimi, K., Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin. Proc. Natl. Acad. Sci. U.S.A. 116 (17): 8301-8309 (2019). PMID 30948637.

2019.10.29

GNP_filter3_RDBDEP_html_190927.pl