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pCAG-EGFP-NpF2164g5_BV4

Expression vector of engineered molecules biliverdin (BV)-binding molecules (NpF2164g5_BV4).

Catalog number RDB17331
Resource name pCAG-EGFP-NpF2164g5_BV4
Alternative name GFP-NpF2164g5_BV4 in pcDNA3
Clone info. Expression vector of engineered molecules biliverdin (BV)-binding molecules (NpF2164g5_BV4), CAG promoter, tagged with GFP at N-terminus.
NpF2164g5_BV4, can function as a near-infrared (NIR) fluorescent probe in vivo, see Fig. 4 of Fushimi, K. et al., Proc. Natl. Acad. Sci. U S A, 116 (17): 8301-8309, 2019. This molecule could be a platform for the further development of brighter NIR fluorescent probes.
Comment The GFP-NpF2164g5_BV4 was cloned into HindIII and XhoI sites of a pcDNA3 (Invitrogen) in which the CMV promoter was replaced with CAG promoter. Expression was confirmed by the depositor with fluorescence imaging.
Vector backbone ocDNA3.1 (modified) (plasmid)
Selectable markers E. coli, Ampicillin; mammalian cell, Neomycin
Gene/insert name Nostoc punctiforme npf2164g5 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Narikawa, Rei |
Other clones in our bank
External Database
Nostoc punctiforme npf2164g5
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            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fushimi, K et al., Proc. Natl. Acad. Sci. USA, 116(17), 8301-8309, 2019)
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fushimi, K et al., Proc. Natl. Acad. Sci. USA, 116(17), 8301-8309, 2019)。
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            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB17331 pCAG-EGFP-NpF2164g5_BV4 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Sequence (full) RDB17331zzk01.seq provided by the depositor

            Original reference

            original Fushimi, K., Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin. Proc. Natl. Acad. Sci. U.S.A. 116 (17): 8301-8309 (2019). PMID 30948637.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Fushimi, K., Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin. Proc. Natl. Acad. Sci. U.S.A. 116 (17): 8301-8309 (2019). PMID 30948637.

            2022.05.17

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