Expression vector of engineered molecules biliverdin (BV)-binding molecules (NpF2164g5_BV4).
Catalog number | RDB17331 |
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Resource name | pCAG-EGFP-NpF2164g5_BV4 |
Alternative name | GFP-NpF2164g5_BV4 in pcDNA3 |
Clone info. | Expression vector of engineered molecules biliverdin (BV)-binding molecules (NpF2164g5_BV4), CAG promoter, tagged with GFP at N-terminus. NpF2164g5_BV4, can function as a near-infrared (NIR) fluorescent probe in vivo, see Fig. 4 of Fushimi, K. et al., Proc. Natl. Acad. Sci. U S A, 116 (17): 8301-8309, 2019. This molecule could be a platform for the further development of brighter NIR fluorescent probes. The GFP-NpF2164g5_BV4 was cloned into HindIII and XhoI sites of a pcDNA3 (Invitrogen) in which the CMV promoter was replaced with CAG promoter. Expression was confirmed by the depositor with fluorescence imaging. |
Vector backbone | ocDNA3.1 (modified) (plasmid) |
Selectable markers | E. coli, Ampicillin; mammalian cell, Neomycin |
Gene/insert name | Nostoc punctiforme npf2164g5 cDNA Aequorea victoria GFP cDNA |
Depositor|Developer | Narikawa, Rei | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fushimi, K et al., Proc. Natl. Acad. Sci. USA, 116(17), 8301-8309, 2019) |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA) [Word ![]() Schedule A (GFP-A) [Link] |
Remarks | Please use Schedule A (GFP-A) additionally. |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB17331 | pCAG-EGFP-NpF2164g5_BV4 | Under QC test. Please contact us. | DNA solution | Please contact us. |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Fluorescent protein resources (English text) |
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Featured content | Fluorescent protein resources (Japanese text) |
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
2020.10.02
GNP_filter3_RDBDEP_html_200316.pl