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pCMV-GRWD1 (#RDB17328)

Expression vector of human GRWD1.

Clone info. Expression vector of human glutamate rich WD repeat containing 1 (GRWD1), CMV promoter. Insert sequence was synthesized, so the sequence is different from RefSeq.
Comment Please note that this clone has non-tag. Expression was confirmed by the depositor with immunoblotting.
Vector backbone pFLAG-CMV-5b (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name human GRWD1 cDNA
Depositor|Developer Fujita, Masatoshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Watanabe, S. et al. J. Cell Sci. 131(15), 2018).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Watanabe, S. et al. J. Cell Sci. 131(15), 2018)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17328 pCMV-GRWD1 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCMV-GRWD1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17328).

Reference section:

Watanabe, S., Fujiyama, H., Takafuji, T., Kayama, K., Matsumoto, M., Nakayama, K.I., Yoshida, K., Sugimoto, N., Fujita, M., GRWD1 regulates ribosomal protein L23 levels via the ubiquitin-proteasome system. J. Cell Sci. 131 (15). pii: jcs213009 (2018). PMID 29991511. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Watanabe, S., GRWD1 regulates ribosomal protein L23 levels via the ubiquitin-proteasome system. J. Cell Sci. 131 (15). pii: jcs213009 (2018). PMID 29991511. [link to RRC of NBRP]