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CSII-EF-IRES2-Venus_hFoxP3

Lentivirus expression vector of human FOXP3.

Catalog number RDB17320
Resource name CSII-EF-IRES2-Venus_hFoxP3
Clone info. Lentivirus expression vector of human forkhead box P3 (FOXP3), EF-1 alpha promoter, Venus.
Comment Expression was confirmed by the deposotor with the observation of Venus expression and western blotting.
Vector backbone CSIIR-EF-MCS-IRES2-Venus (plasmid)
Size of vector backbone 10.0 kb
Selectable markers Amp^r.
Gene/insert name human FOXP3 cDNA
Depositor|Developer Yoshitomi, Hiroyuki |
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External Database
human FOXP3

          Reference sequence
            
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kobayashi S. et al. Eur. J. Immunol. 46(2), 360-371, 2016). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
          Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
          Ordering Please visit Ordering instruction.[link] 
          Order form 
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          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kobayashi S. et al. Eur. J. Immunol. 46(2), 360-371, 2016)。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
          付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          宮脇敦史博士による提供承諾書 [Word]
          備考
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB17320 CSII-EF-IRES2-Venus_hFoxP3 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
          Ordering instruction of plasmids [in Japanese] [in English]

          References and tips

          Electronic file

          Original reference

          original Kobayashi, S., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Kobayashi, S., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894.

          2022.05.17

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