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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-IRES2-Venus_hMAF

Lentivirus expression vector of human MAF, transcript variant 1.

Catalog number RDB17306
Resource name CSII-EF-IRES2-Venus_hMAF
Clone info. Lentivirus expression vector of human MAF bZIP transcription factor (MAF), transcript variant 1, EF-1 alpha promoter, Venus.
Comment Expression was confirmed by the deposotor with the observation of Venus expression and western blotting.
Vector backbone CSII-EF-RfA-IRES2-Venus (plasmid)
Size of vector backbone 10 kb
Selectable markers Amp^r.
Gene/insert name human MAF cDNA
Depositor|Developer Yoshitomi, Hiroyuki |
Other clones in our bank

External Database
human MAF

          Reference sequence
            
           
          Sequence (full) RDB17306zzk01.seq provided by the depositor
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yoshitomi H. et al. Nat. Commun. 9(1), 3762, 2018). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a teaching and academic research purpose.
          Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
          Ordering Please visit Ordering instruction.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yoshitomi H. et al. Nat. Commun. 9(1), 3762, 2018)。リソースの使用は、非営利機関での非営利学術・研究目的に限定する。
          付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          宮脇敦史博士による提供承諾書 [Word]
          備考
          宮脇敦史博士の書面による提供承諾が必要です。
          Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB17306 CSII-EF-IRES2-Venus_hMAF DNA solution

          Ordering instruction of plasmids [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Yoshitomi, H., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17306_B2Hfp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Region: insert 5'(attB1,hMAF 5')
          Sequence file: RDB17306_B2Hfa.seq check
          >D06220B9_B2Hf_1_EF1a-C_F_A09_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN TNNCCTTTTT NANTTTGGNN CTTGGTTCNT TCTNNAAGCC
             61 TCAGACAGTG GTTCAAANNN NNNNTCTTCC ATTTCAGGTG TCGTGAACAC GCTACCGGTC
            121 NCNAGAATTC TGCAGCGGCC ATCACAAGTT TGTACAAAAA AGCAGGCTCC ACCATGGGAA
            181 CCAATTCAGT CGACTGGATC CGCCACCATG GCATCAGAAC TGGCAATGAG CAACTCCGAC
            241 CTGCCCACCA GTCCCCTGGC CATGGAATAT GTTAATGACT TCGATCTGAT GAAGTTTGAA
            301 GTGAAAAAGG AACCGGTGGA GACCGACCGC ATCATCAGCC AGTGCGGCCG TCTCATCGCC
            361 GGGGGCTCGC TGTCCTCCAC CCCCATGAGC ACGCCGTGCA GCTCGGTGCC CCCTTCCCCC
            421 AGCTTCTCGG CGCCCAGCCC GGGCTCGGGC AGCGAGCAGA AGGCGCACCT GGAAGACTAC
            481 TACTGGATGA CCGGCTACCC GCAGCAGCTG AACCCCGAGG CGCTGGGCTT CAGCCCCGAG
            541 GACGCGGTCG AGGCGCTCAT CAGCAACAGC CACCAGCTCC AGGGCGGCTT CGATGGCTAC
            601 GCGCGCGGGG CGCAGCAGCT GGCCGCGGCG GCCGGGGCCG GTGCCGGCGC CTCCTTGGGC
            661 GGCAGCGGCG AGGAGATGGG CCCCGCCGCC GCCGTGGTGT CCGCCGTGAT CGCCGCGGCC
            721 GCCGCGCAGA GCGGCGCGGG CCCGCACTAC CACCACCACC ACCACCACGC CGCCGGCCAC
            781 CACCACCACC CGACGGCCGG CGCGCCCGGC GCCGCGGGCA NCGCGGCCGC CTCGGCCGGT
            841 GGCGCTGGGG GCGCGGGCGG CGGTGGCCCN GCCAGCGCTG GGGGCGGCGG CGGCGGCGGC
            901 NGCGGCNNAN GCGGCGGGGG CNCGGCGGGG GCGGGGGGGC NCCCTGCACC CNCACCACGC
            961 CGCCNGCGGC CNGCACNTNC GNNNANNNNN TNNNNGANNN NCANCNNGNG NNCNNNGNNN
          1021 NNNCGCNANC NNNANNNNGN NNNCNNCNCN GGNNNNNCNN NNNNNNNN
          //
          Primer: hMAF-B_F
          Region: insert 3'(hMAF 3',attB2),IRES2
          Sequence file: RDB17306_B2Hfb.seq check
          >D06220B9_B2Hf_1_hMAF-B_F_C03_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN CCTGCCGCTT CAAGAGGGTG CAGCAGAGAC ACGTCCTGGA
             61 GTCGGAGAAN ANCNNNNTGC TGCAGCAAGT CGACCACCTC AAGCAGGAGA TCTCCAGGCT
            121 GGTGCGCGAG AGGGACGCGT ACAAGGAGAA ATACGAGAAG TTGGTGAGCA GCGGCTTCCG
            181 AGAAAACGGC TCGAGCAGCG ACAACCCGTC CTCTCCCGAG TTTTTCATAA CTGAGCCCAC
            241 TCGCAAGTTG GAGCCATCAG TGGGATACGC CACATTTTGG AAGCCCCAGC ATCGTGTACT
            301 TACCAGTGTG TTCACAAAAT GAAATTTGTG TGAGAGCTGT ACATTAAAAA AAATCATCAT
            361 TATTATTATT ATTTGCAGTC ATGGAGAACC ACCTACCCCT CTAGACCCAG CTTTCTTGTA
            421 CAAAGTGGTG ATGGCCGCGG ATCCGCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC
            481 GAAGCCGCTT GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC
            541 CGTCTTTTGG CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCNTTCCTA
            601 GGGGTCTTTN CCCTCTCGCC AAAGGAATGC AAGGTCTGNT GAATGTCGTG ANNNAAGCAG
            661 TTCCTCTGGN ANCNTCTNGA NNNNAACANN NTNNGNANCG ACCNNTTGNN NNNNCNNNNN
          721 CCCCNNNNNN NNNN
          //
          Primer: PRE_rev
          Region: mVenus 3'
          Sequence file: RDB17306_B2Hfc.seq check
          >D06220B9_B2Hf_1_PRE_rev_B09_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNANCAACA TAGTTAAGAA NACCNGTCAA TCTTTCNCNA
             61 ATTTTGTAAT NNNNNNNNNN NNNANCNATA AGCTTGATCC CTCGATGTTN NCNNNNNAGG
            121 CTCGAGAGGC CTTGAATTCT TACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG
            181 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG
            241 CGGACTGGTA GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
            301 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCCGCCGTC CTCGATGTTG TGGCGGATCT
            361 TGAAGTTGGC CTTGATGCCG TTCTTCTGCT TGTCGGCGGT GATATAGACG TTGTGGCTGT
            421 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT
            481 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT
            541 AGTTGCCGTC GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG
            601 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GGCGAAGCAC TGCAGGCCGT
            661 AGCCCAGGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATCA
            721 GCTTCAGGGT CAGCTTGCCG TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT
            781 TGTGGCCGTT TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT
            841 CCTCGCCCTT GCTCACCATG GTTGTGGNCA TATTATCATC GTGTTTTTTC AAAGGAAAAN
            901 CACGTCCCCG TGGTTCNGGG GGGCCTANAC GTTTTTTTTN NCCTCGACTN NNNCATGTAA
            961 AGCATGNGTA CCNNNGNCCC CNNNNNNNNT NCCNTANNNN GGGGGNNNNN NNNGGGNNNN
          1021 NNNNNN
          //
          Primer: CMV_Forward
          Region: 5'LTR,HIV-1 psi
          Sequence file: RDB17306_B2Hfd.seq check
          >D06220B9_B2Hf_1_CMV_Forward_E09_15_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
             61 NNTNNNNNNG CAGCNCNNTT TGNCTGTACT GGGTCTCTCT GGTTAGACCA GATNNNNNNC
            121 TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA
            181 GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA
            241 CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA
            301 AAGGGAAACC AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA
            361 GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTANAANG
            421 AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA
            481 AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA
            541 GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA
            601 GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT
            661 TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA
            721 AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG
            781 GGCCGCTGAT CTTCAGACCT GGAGGANGAN ATATGANGGA CAATTGGANA AGTGAATTAT
            841 ATAAATATAA AGTAGTAAAA ATTGAACCAT TANGANTAGC ACCCACCAAG GCAAANAGAA
            901 GAGTGGTGCA GANNGAAAAA AAGANCAGTG GGAATAGGAN CTTTGNTCCN NGGGNTCNTN
          961 GGGAGCAGCN NNNNNCNNTA NNGGNNGCNN CGNNNNTNNN NCNNNNNGNN NNNNNNNN
          //
          Primer: HIVgp_R1
          Region: HIV-1 psi,5'LTR
          Sequence file: RDB17306_B2Hfe.seq check
          >D06220B9_B2Hf_1_HIVgp_R1_F09_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGCT CTTTTTTCTC TCTGCNCCAC TCTTCTCTTT GCCTTGGTGG
             61 GTGCTNNNNN NNNNGGTTNA ATTTTTACTA CTTTATATTT ATATAATTCN NNTCTCCAAT
            121 TGTCCCTCAT ATCTCCTCCT CCAGGTCTGA AGATCAGCGG CCCTTGCTGT GCGGTGGTCT
            181 TACTTTTGTT TTGCTCTTCC TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA
            241 TCCTTTGATG CACACAATAG AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG
            301 ATCCTGTCTG AAGGGATGGT TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT
            361 CTAACAGGCC AGGATTAACT GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT
            421 TTAATTTATA TTTTTTCTTT CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA
            481 TTCTCCCCCG CTTAATACTG ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG
            541 TCAAAATTTT TGGCGTACTC ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG
            601 CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC TTTCGCTTTC AAGTCCCTGT
            661 TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGAGGG ATCTCTAGTT
            721 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG
            781 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCANA TCTGGTCTAA
            841 CCAGAGAGAN CCAGTACAGG CAAAACGCGC TGCTTATATA GACCTCCCAC CGNACACGCC
            901 TACCGCCCAT TTGCGTCNAT GGGGCGGNNT TGTTANNANA TTTTGGAAAN TCCCGTTNAN
          961 TTTGGTGCNN AAACAAACNN NNNNNNNN
          //
          Primer: EF1a-N_R
          Region: cPPT_CTS
          Sequence file: RDB17306_B2Hff.seq check
          >D06220B9_B2Hf_1_EF1a-N_R_G09_21_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GCGNNGTGCG CTCTGCCCAC TGACGGGCAC CGGANCCTCA
             61 CGAAGCNNNN NNNNNNTCAN AGTGAATTTC TGCTGTCCCT GTAATAAACC CNNNNATTTT
            121 GAATTTTTGT AATTTGTTTT TGTAATTCTT TAGTTTGTAT GTCTGTTGCT ATTATGTCTA
            181 CTATTCTTTC CCCTGCACTG TACCCCCCAA TCCCCCCTTT TCTTTTAAAA TTGTGGATGA
            241 ATACTGCCAT TTGTAGATCC GTTCACTAAT CGAATGGATC TGTCTCTGTC TCTCTCTCCA
            301 CCTTCTTCTT CTATTCCTTC GGGCCTGTCG GGTCCCCTCG GGGTTGGGAG GTGGGTCTGA
            361 AACGATAATG GTGAATATCC CTGCCTAACT CTATTCACTA TAGAAAGTAC NNCNNAAACT
            421 ATTCTTAAAC CTACCAAGCC TCCTACTATC ATTATGAATA ATTTTATATA CCACAGCCAA
            481 TTTGTTATGT TAAACCAATT CCACAAACTT GCCCATTTAT CTAATTCCAA TAATTCTTGT
            541 TCATTCTTTT CTTGCTGGTT TTGCGATTCT TCAATTAAGG AGTGTATTAA GCTTGTGTAA
            601 TTGTTAATTT CTCTGTCCCA CTCCATCCAG GTCGTGTGAT TCCAAATCTG TTCCAGAGAT
            661 TTATTACTCC AACTAGCATT CCAAGGCACA GCAGTGGTGC AAATGAGTTT TCCAGAGCAA
            721 CCCCAAATCC CCAGGAGCTG TTGATCCTTT ANGTATCTTT CCACAGCCAG GATTCTTGCC
            781 TGGANCTGCT TGATGCCCCA GANTGTGANT TGCAACAGAT GCTGTTGCGC CTCAATANCC
            841 CTCANCAAAT TGTTCTGCTG CTGCACTATA NCANACAATA ATTGTCTGGN CNGTACCGTC
            901 AGCGTCATNG ACGCTGCNCC CATAGTGCTT CCNGCTGCTC CCNANANNCN NNNNNNNAAA
            961 GCTCNNNNTN CCACNNNNNN TTTTTNNTCT CTNNNNNNAC NNNNNNNNTN NNNNTNNNNN
          1021 NNNNN
          //
          Primer: EGFP-M_R
          Region: mVenus 5',IRES2
          Sequence file: RDB17306_B2Hfg.seq check
          >D06220B9_B2Hf_1_EGFP-M_R_non_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNG NGGNGGNNCT TGNAGTTGGC CTTGNTGCCG TTCTTCTGCT
             61 TGTCGGCNNN NNNNNNNNNN NTTGTGGCTG TTGTAGTTGT ACTCCAGCTT GTGCCCNNNG
            121 ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG
            181 CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG
            241 CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG
            301 TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG TAGCCCAGGG TGGTCACGAG GGTGGGCCAG
            361 GGCACGGGCA GCTTGCCGGT GGTGCAGATC AGCTTCAGGG TCAGCTTGCC GTAGGTGGCA
            421 TCGCCCTCGC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG
            481 ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT GGTTGTGGCC
            541 ATATTATCAT CGTGTTTTTC ANANGAAAAC CACGTCCCCG TGGTTCGGGG GGCCTAGACG
            601 TTTTTTTAAC CTCGACTAAA CACATGTAAA GCATGTGTAC CGAGGCCCCN NATCNNATCC
            661 CATACAATGG GGTACCTTCT GGGCATCCTT CANCCCCTTG TTGAATACGC TTGAGGANAN
            721 NNATTTGACT CNTTTCCACA ACTATCCNAC TCACAACGTG GCACTGGGGT TGTGCCGCCT
            781 TTGCANGTGT ATCTTATACA CGTGGCTTTT GGCCNCNNNN GCACCTGTCG CCNGGTGGGG
            841 GGGTTCCGCT NNNNTGNNAA GGGTCGCTAC NNACNTTNNT TNNTNNTTCN ANAANNNNTT
            901 NNNNNANGAN CTGCTTNCCT TNNNCNANNT TCNNNANNNN NNTNGNNTTC CNTTNGNNNA
            961 NAGGGGNAAA NANCNNCTNN GNANNGCNNN NNNNNNANNN NNNNGGNNNN NNGNTTNCNG
          1021 GGNNCNNNNN NNNTTNNNNN AAANNANNGG NNNNNNNNGN NNGNNN
          //
          Primer: bGH_rev3
          Region: 3'LTR
          Sequence file: RDB17306_B2Hfh.seq check
          >D06220B9_B2Hf_1_bGH_rev3_H09_24_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN NGNNGGNNNN NNACNGNNGG CTGGCAACTA GAAGGCACAG
             61 TCGAGGNNNN NNNNNGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCANNN NNNNTAAAAG
            121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC
            181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC
            241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC
            301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA
            361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGNNTTCT
            421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT
            481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG
            541 ATCCACAGAT CAANGATATC TTGTCTTCNT NNGGANTGAA TTANCCCTTC CNNTCCCCCC
            601 TTTTCTTTTA AAAAGTGGCT NANANCTACA GCTGCCNTGT ANGTCNTNGG TCTTNAANNN
          661 NACNTGNNNG NNACTNNNAA NNCCANNTNN NNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: EM7 pro,Zeo
          Sequence file: RDB17306_B2Hfi.seq check
          >D06220B9_B2Hf_1_SV40pro_ori_F_B08_05_ABI24.ab1
              1 NNNNNNNNNN NNNNGCCGCC TCTGCCTCTG ANCTATTCNN GAAGTAGTGA GGAGGCTTTT
             61 TTGNNNNNNN NNGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCNTT TTCGGATCTG
            121 ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA
            181 GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA
            241 CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA
            301 GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA
            361 CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA
            421 CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC
            481 CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG
            541 CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC
            601 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT
            661 CCAGCGCGGG GNTCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA
            721 TAATGNTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT
            781 GCATTCTANT TGTGGTTTGT CCAAACTCAT CNATGTATCT TATCATGTCT GTATACCGTC
            841 NANCTCTANC TAGANCTNNN NNTAATCATG GTCATANCNG NTNNCTGNGN GAAATTGNTA
            901 TCCNCTCNNA ATTCCNCNCN NNNTACNANC NNGNNNNNNN AAGTGNAAAN NCNNGGGNNN
          961 NNTNNNGANN NNNCNNNNNN NNNN
          //
          Primer: hMAF-B_F3
          Region: insert 3'(hMAF 3',attB2),IRES2
          Sequence file: RDB17306_B2Hfj.seq check
          >D06220B9_B2Hf_1_hMAF-B_F3_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNCCGNN NGCCGGCGCG CCCGGCGCCG CGGGCAGCGC GGCCGCCTCG
             61 GCCGGTGGCG CTGGGGGCGC GGGCGGCGGT GGCCCGGCCA GCGCTGGGGG CGGCGGCGGC
            121 GGCGGCGGCG GCGGAGGCGG CGGGGGCGCG GCGGGGGCGG GGGGCGCCCT GCACCCGCAC
            181 CACGCCGCCG GCGGCCTGCA CTTCGACGAC CGCTTCTCCG ACGAGCAGCT GGTGACCATG
            241 TCTGTGCGCG AGCTGAACCG GCAGCTGCGC GGGGTCAGCA AGGAGGAGGT GATCCGGCTG
            301 AAGCAGAAGA GGCGGACCCT GAAAAACCGC GGCTATGCCC AGTCCTGCCG CTTCAAGAGG
            361 GTGCAGCAGA GACACGTCCT GGAGTCGGAG AAGAACCAGC TGCTGCAGCA AGTCGACCAC
            421 CTCAAGCAGG AGATCTCCAG GCTGGTGCGC GAGAGGGACG CGTACAAGGA GAAATACGAG
            481 AAGTTGGTGA GCAGCGGCTT CCGAGAAAAC GGCTCGAGCA GCGACAACCC GTCCTCTCCC
            541 GAGTTTTTCA TAACTGAGCC CACTCGCAAG TTGGAGCCAT CAGTGGGATA CGCCACATTT
            601 TGGAAGCCCC AGCATCGTGT ACTTACCAGT GTGTTCACAA AATGAAATTT GTGTGAGAGC
            661 TGTACATTAA AAAAAATCAT CATTATTATT ATTATTTGCA GTCATGGAGA ACCACCTACC
            721 CCTCTAGACC CAGCTTTCTT GTACAAAGTG GTGATGGCCG CGGATCCGCC CCTCTCCCTC
            781 CCCCCCCCCT AACGTTACTG GCCGAAGCCG CTTGGAATAA GGCCGGTGTG CGTTTGTCTA
            841 TATGTTATTT TCCACCATAT TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AANCTGGCCC
            901 TGNCTTCTTG ACNANCATTC CNANGGGTCT TTCCCCNNNC GCCNAANGNA TGCNNGGNCT
          961 GTTGANTGNC NNNNNNGANG CANTNNNNNT NGNNNNCNTN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yoshitomi, H., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328.

          2022.09.29

          GNP_filter3_RDBDEP_html_220812.pl