RDB17306 - CSII-EF-IRES2-Venus

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CSII-EF-IRES2-Venus_hMAF (#RDB17306)

Lentivirus expression vector of human MAF, transcript variant 1.

Clone info.	Lentivirus expression vector of human MAF bZIP transcription factor (MAF), transcript variant 1, EF-1 alpha promoter, Venus.
Comment	Expression was confirmed by the deposotor with the observation of Venus expression and western blotting.
Vector backbone	CSII-EF-RfA-IRES2-Venus (RDB04389) (plasmid)
Size of vector backbone	10 kb
Selectable markers	Amp^r.
Gene/insert name	human MAF cDNA
Depositor\|Developer	Yoshitomi, Hiroyuki \|

Sequence (full)	RDB17306zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yoshitomi H. et al. Nat. Commun. 9(1), 3762, 2018). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a teaching and academic research purpose.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.

Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yoshitomi H. et al. Nat. Commun. 9(1), 3762, 2018). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a teaching and academic research purpose. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks	Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yoshitomi H. et al. Nat. Commun. 9(1), 3762, 2018)。リソースの使用は、非営利機関での非営利学術・研究目的に限定する。 MTAに書く付加的使用条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考	Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB17306	CSII-EF-IRES2-Venus_hMAF	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

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How to cite this biological resource

Materials & Methods section:

The CSII-EF-IRES2-Venus_hMAF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17306).

Reference section:

Yoshitomi, H., Kobayashi, S., Miyagawa-Hayashino, A., Okahata, A., Doi, K., Nishitani, K., Murata, K., Ito, H., Tsuruyama, T., Haga, H., Matsuda, S., Toguchida, J., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17306_B2Hfp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: insert 5'(attB1,hMAF 5') Sequence file: RDB17306_B2Hfa.seq
>D06220B9_B2Hf_1_EF1a-C_F_A09_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN TNNCCTTTTT NANTTTGGNN CTTGGTTCNT TCTNNAAGCC 61 TCAGACAGTG GTTCAAANNN NNNNTCTTCC ATTTCAGGTG TCGTGAACAC GCTACCGGTC 121 NCNAGAATTC TGCAGCGGCC ATCACAAGTT TGTACAAAAA AGCAGGCTCC ACCATGGGAA 181 CCAATTCAGT CGACTGGATC CGCCACCATG GCATCAGAAC TGGCAATGAG CAACTCCGAC 241 CTGCCCACCA GTCCCCTGGC CATGGAATAT GTTAATGACT TCGATCTGAT GAAGTTTGAA 301 GTGAAAAAGG AACCGGTGGA GACCGACCGC ATCATCAGCC AGTGCGGCCG TCTCATCGCC 361 GGGGGCTCGC TGTCCTCCAC CCCCATGAGC ACGCCGTGCA GCTCGGTGCC CCCTTCCCCC 421 AGCTTCTCGG CGCCCAGCCC GGGCTCGGGC AGCGAGCAGA AGGCGCACCT GGAAGACTAC 481 TACTGGATGA CCGGCTACCC GCAGCAGCTG AACCCCGAGG CGCTGGGCTT CAGCCCCGAG 541 GACGCGGTCG AGGCGCTCAT CAGCAACAGC CACCAGCTCC AGGGCGGCTT CGATGGCTAC 601 GCGCGCGGGG CGCAGCAGCT GGCCGCGGCG GCCGGGGCCG GTGCCGGCGC CTCCTTGGGC 661 GGCAGCGGCG AGGAGATGGG CCCCGCCGCC GCCGTGGTGT CCGCCGTGAT CGCCGCGGCC 721 GCCGCGCAGA GCGGCGCGGG CCCGCACTAC CACCACCACC ACCACCACGC CGCCGGCCAC 781 CACCACCACC CGACGGCCGG CGCGCCCGGC GCCGCGGGCA NCGCGGCCGC CTCGGCCGGT 841 GGCGCTGGGG GCGCGGGCGG CGGTGGCCCN GCCAGCGCTG GGGGCGGCGG CGGCGGCGGC 901 NGCGGCNNAN GCGGCGGGGG CNCGGCGGGG GCGGGGGGGC NCCCTGCACC CNCACCACGC 961 CGCCNGCGGC CNGCACNTNC GNNNANNNNN TNNNNGANNN NCANCNNGNG NNCNNNGNNN 1021 NNNCGCNANC NNNANNNNGN NNNCNNCNCN GGNNNNNCNN NNNNNNNN //
Primer: hMAF-B_F (Pr1240) Region: insert 3'(hMAF 3',attB2),IRES2 Sequence file: RDB17306_B2Hfb.seq
>D06220B9_B2Hf_1_hMAF-B_F_C03_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN CCTGCCGCTT CAAGAGGGTG CAGCAGAGAC ACGTCCTGGA 61 GTCGGAGAAN ANCNNNNTGC TGCAGCAAGT CGACCACCTC AAGCAGGAGA TCTCCAGGCT 121 GGTGCGCGAG AGGGACGCGT ACAAGGAGAA ATACGAGAAG TTGGTGAGCA GCGGCTTCCG 181 AGAAAACGGC TCGAGCAGCG ACAACCCGTC CTCTCCCGAG TTTTTCATAA CTGAGCCCAC 241 TCGCAAGTTG GAGCCATCAG TGGGATACGC CACATTTTGG AAGCCCCAGC ATCGTGTACT 301 TACCAGTGTG TTCACAAAAT GAAATTTGTG TGAGAGCTGT ACATTAAAAA AAATCATCAT 361 TATTATTATT ATTTGCAGTC ATGGAGAACC ACCTACCCCT CTAGACCCAG CTTTCTTGTA 421 CAAAGTGGTG ATGGCCGCGG ATCCGCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC 481 GAAGCCGCTT GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC 541 CGTCTTTTGG CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCNTTCCTA 601 GGGGTCTTTN CCCTCTCGCC AAAGGAATGC AAGGTCTGNT GAATGTCGTG ANNNAAGCAG 661 TTCCTCTGGN ANCNTCTNGA NNNNAACANN NTNNGNANCG ACCNNTTGNN NNNNCNNNNN 721 CCCCNNNNNN NNNN //
Primer: PRE_rev (Pr0250) Region: mVenus 3' Sequence file: RDB17306_B2Hfc.seq
>D06220B9_B2Hf_1_PRE_rev_B09_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNANCAACA TAGTTAAGAA NACCNGTCAA TCTTTCNCNA 61 ATTTTGTAAT NNNNNNNNNN NNNANCNATA AGCTTGATCC CTCGATGTTN NCNNNNNAGG 121 CTCGAGAGGC CTTGAATTCT TACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG 181 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG 241 CGGACTGGTA GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG 301 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCCGCCGTC CTCGATGTTG TGGCGGATCT 361 TGAAGTTGGC CTTGATGCCG TTCTTCTGCT TGTCGGCGGT GATATAGACG TTGTGGCTGT 421 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT 481 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT 541 AGTTGCCGTC GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG 601 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GGCGAAGCAC TGCAGGCCGT 661 AGCCCAGGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATCA 721 GCTTCAGGGT CAGCTTGCCG TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT 781 TGTGGCCGTT TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT 841 CCTCGCCCTT GCTCACCATG GTTGTGGNCA TATTATCATC GTGTTTTTTC AAAGGAAAAN 901 CACGTCCCCG TGGTTCNGGG GGGCCTANAC GTTTTTTTTN NCCTCGACTN NNNCATGTAA 961 AGCATGNGTA CCNNNGNCCC CNNNNNNNNT NCCNTANNNN GGGGGNNNNN NNNGGGNNNN 1021 NNNNNN //
Primer: CMV_Forward (Pr0016) Region: 5'LTR,HIV-1 psi Sequence file: RDB17306_B2Hfd.seq
>D06220B9_B2Hf_1_CMV_Forward_E09_15_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 61 NNTNNNNNNG CAGCNCNNTT TGNCTGTACT GGGTCTCTCT GGTTAGACCA GATNNNNNNC 121 TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA 181 GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA 241 CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA 301 AAGGGAAACC AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA 361 GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTANAANG 421 AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA 481 AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA 541 GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA 601 GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT 661 TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA 721 AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG 781 GGCCGCTGAT CTTCAGACCT GGAGGANGAN ATATGANGGA CAATTGGANA AGTGAATTAT 841 ATAAATATAA AGTAGTAAAA ATTGAACCAT TANGANTAGC ACCCACCAAG GCAAANAGAA 901 GAGTGGTGCA GANNGAAAAA AAGANCAGTG GGAATAGGAN CTTTGNTCCN NGGGNTCNTN 961 GGGAGCAGCN NNNNNCNNTA NNGGNNGCNN CGNNNNTNNN NCNNNNNGNN NNNNNNNN //
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi,5'LTR Sequence file: RDB17306_B2Hfe.seq
>D06220B9_B2Hf_1_HIVgp_R1_F09_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGCT CTTTTTTCTC TCTGCNCCAC TCTTCTCTTT GCCTTGGTGG 61 GTGCTNNNNN NNNNGGTTNA ATTTTTACTA CTTTATATTT ATATAATTCN NNTCTCCAAT 121 TGTCCCTCAT ATCTCCTCCT CCAGGTCTGA AGATCAGCGG CCCTTGCTGT GCGGTGGTCT 181 TACTTTTGTT TTGCTCTTCC TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA 241 TCCTTTGATG CACACAATAG AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG 301 ATCCTGTCTG AAGGGATGGT TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT 361 CTAACAGGCC AGGATTAACT GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT 421 TTAATTTATA TTTTTTCTTT CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA 481 TTCTCCCCCG CTTAATACTG ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG 541 TCAAAATTTT TGGCGTACTC ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG 601 CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC TTTCGCTTTC AAGTCCCTGT 661 TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGAGGG ATCTCTAGTT 721 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG 781 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCANA TCTGGTCTAA 841 CCAGAGAGAN CCAGTACAGG CAAAACGCGC TGCTTATATA GACCTCCCAC CGNACACGCC 901 TACCGCCCAT TTGCGTCNAT GGGGCGGNNT TGTTANNANA TTTTGGAAAN TCCCGTTNAN 961 TTTGGTGCNN AAACAAACNN NNNNNNNN //
Primer: EF1a-N_R (Pr0024) Region: cPPT_CTS Sequence file: RDB17306_B2Hff.seq
>D06220B9_B2Hf_1_EF1a-N_R_G09_21_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GCGNNGTGCG CTCTGCCCAC TGACGGGCAC CGGANCCTCA 61 CGAAGCNNNN NNNNNNTCAN AGTGAATTTC TGCTGTCCCT GTAATAAACC CNNNNATTTT 121 GAATTTTTGT AATTTGTTTT TGTAATTCTT TAGTTTGTAT GTCTGTTGCT ATTATGTCTA 181 CTATTCTTTC CCCTGCACTG TACCCCCCAA TCCCCCCTTT TCTTTTAAAA TTGTGGATGA 241 ATACTGCCAT TTGTAGATCC GTTCACTAAT CGAATGGATC TGTCTCTGTC TCTCTCTCCA 301 CCTTCTTCTT CTATTCCTTC GGGCCTGTCG GGTCCCCTCG GGGTTGGGAG GTGGGTCTGA 361 AACGATAATG GTGAATATCC CTGCCTAACT CTATTCACTA TAGAAAGTAC NNCNNAAACT 421 ATTCTTAAAC CTACCAAGCC TCCTACTATC ATTATGAATA ATTTTATATA CCACAGCCAA 481 TTTGTTATGT TAAACCAATT CCACAAACTT GCCCATTTAT CTAATTCCAA TAATTCTTGT 541 TCATTCTTTT CTTGCTGGTT TTGCGATTCT TCAATTAAGG AGTGTATTAA GCTTGTGTAA 601 TTGTTAATTT CTCTGTCCCA CTCCATCCAG GTCGTGTGAT TCCAAATCTG TTCCAGAGAT 661 TTATTACTCC AACTAGCATT CCAAGGCACA GCAGTGGTGC AAATGAGTTT TCCAGAGCAA 721 CCCCAAATCC CCAGGAGCTG TTGATCCTTT ANGTATCTTT CCACAGCCAG GATTCTTGCC 781 TGGANCTGCT TGATGCCCCA GANTGTGANT TGCAACAGAT GCTGTTGCGC CTCAATANCC 841 CTCANCAAAT TGTTCTGCTG CTGCACTATA NCANACAATA ATTGTCTGGN CNGTACCGTC 901 AGCGTCATNG ACGCTGCNCC CATAGTGCTT CCNGCTGCTC CCNANANNCN NNNNNNNAAA 961 GCTCNNNNTN CCACNNNNNN TTTTTNNTCT CTNNNNNNAC NNNNNNNNTN NNNNTNNNNN 1021 NNNNN //
Primer: EGFP-M_R (Pr0743) Region: mVenus 5',IRES2 Sequence file: RDB17306_B2Hfg.seq
>D06220B9_B2Hf_1_EGFP-M_R_non_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNG NGGNGGNNCT TGNAGTTGGC CTTGNTGCCG TTCTTCTGCT 61 TGTCGGCNNN NNNNNNNNNN NTTGTGGCTG TTGTAGTTGT ACTCCAGCTT GTGCCCNNNG 121 ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG 181 CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG 241 CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG 301 TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG TAGCCCAGGG TGGTCACGAG GGTGGGCCAG 361 GGCACGGGCA GCTTGCCGGT GGTGCAGATC AGCTTCAGGG TCAGCTTGCC GTAGGTGGCA 421 TCGCCCTCGC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG 481 ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT GGTTGTGGCC 541 ATATTATCAT CGTGTTTTTC ANANGAAAAC CACGTCCCCG TGGTTCGGGG GGCCTAGACG 601 TTTTTTTAAC CTCGACTAAA CACATGTAAA GCATGTGTAC CGAGGCCCCN NATCNNATCC 661 CATACAATGG GGTACCTTCT GGGCATCCTT CANCCCCTTG TTGAATACGC TTGAGGANAN 721 NNATTTGACT CNTTTCCACA ACTATCCNAC TCACAACGTG GCACTGGGGT TGTGCCGCCT 781 TTGCANGTGT ATCTTATACA CGTGGCTTTT GGCCNCNNNN GCACCTGTCG CCNGGTGGGG 841 GGGTTCCGCT NNNNTGNNAA GGGTCGCTAC NNACNTTNNT TNNTNNTTCN ANAANNNNTT 901 NNNNNANGAN CTGCTTNCCT TNNNCNANNT TCNNNANNNN NNTNGNNTTC CNTTNGNNNA 961 NAGGGGNAAA NANCNNCTNN GNANNGCNNN NNNNNNANNN NNNNGGNNNN NNGNTTNCNG 1021 GGNNCNNNNN NNNTTNNNNN AAANNANNGG NNNNNNNNGN NNGNNN //
Primer: bGH_rev3 (Pr0885) Region: 3'LTR Sequence file: RDB17306_B2Hfh.seq
>D06220B9_B2Hf_1_bGH_rev3_H09_24_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGN NGNNGGNNNN NNACNGNNGG CTGGCAACTA GAAGGCACAG 61 TCGAGGNNNN NNNNNGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCANNN NNNNTAAAAG 121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC 181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC 241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC 301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA 361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGNNTTCT 421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT 481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG 541 ATCCACAGAT CAANGATATC TTGTCTTCNT NNGGANTGAA TTANCCCTTC CNNTCCCCCC 601 TTTTCTTTTA AAAAGTGGCT NANANCTACA GCTGCCNTGT ANGTCNTNGG TCTTNAANNN 661 NACNTGNNNG NNACTNNNAA NNCCANNTNN NNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: EM7 pro,Zeo Sequence file: RDB17306_B2Hfi.seq
>D06220B9_B2Hf_1_SV40pro_ori_F_B08_05_ABI24.ab1 1 NNNNNNNNNN NNNNGCCGCC TCTGCCTCTG ANCTATTCNN GAAGTAGTGA GGAGGCTTTT 61 TTGNNNNNNN NNGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCNTT TTCGGATCTG 121 ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA 181 GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA 241 CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA 301 GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA 361 CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA 421 CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC 481 CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG 541 CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC 601 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT 661 CCAGCGCGGG GNTCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA 721 TAATGNTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 781 GCATTCTANT TGTGGTTTGT CCAAACTCAT CNATGTATCT TATCATGTCT GTATACCGTC 841 NANCTCTANC TAGANCTNNN NNTAATCATG GTCATANCNG NTNNCTGNGN GAAATTGNTA 901 TCCNCTCNNA ATTCCNCNCN NNNTACNANC NNGNNNNNNN AAGTGNAAAN NCNNGGGNNN 961 NNTNNNGANN NNNCNNNNNN NNNN //
Primer: hMAF-B_F3 (Pr1246) Region: insert 3'(hMAF 3',attB2),IRES2 Sequence file: RDB17306_B2Hfj.seq
>D06220B9_B2Hf_1_hMAF-B_F3_B03_06_ABI24.ab1 1 NNNNNNNNNN NNNNNCCGNN NGCCGGCGCG CCCGGCGCCG CGGGCAGCGC GGCCGCCTCG 61 GCCGGTGGCG CTGGGGGCGC GGGCGGCGGT GGCCCGGCCA GCGCTGGGGG CGGCGGCGGC 121 GGCGGCGGCG GCGGAGGCGG CGGGGGCGCG GCGGGGGCGG GGGGCGCCCT GCACCCGCAC 181 CACGCCGCCG GCGGCCTGCA CTTCGACGAC CGCTTCTCCG ACGAGCAGCT GGTGACCATG 241 TCTGTGCGCG AGCTGAACCG GCAGCTGCGC GGGGTCAGCA AGGAGGAGGT GATCCGGCTG 301 AAGCAGAAGA GGCGGACCCT GAAAAACCGC GGCTATGCCC AGTCCTGCCG CTTCAAGAGG 361 GTGCAGCAGA GACACGTCCT GGAGTCGGAG AAGAACCAGC TGCTGCAGCA AGTCGACCAC 421 CTCAAGCAGG AGATCTCCAG GCTGGTGCGC GAGAGGGACG CGTACAAGGA GAAATACGAG 481 AAGTTGGTGA GCAGCGGCTT CCGAGAAAAC GGCTCGAGCA GCGACAACCC GTCCTCTCCC 541 GAGTTTTTCA TAACTGAGCC CACTCGCAAG TTGGAGCCAT CAGTGGGATA CGCCACATTT 601 TGGAAGCCCC AGCATCGTGT ACTTACCAGT GTGTTCACAA AATGAAATTT GTGTGAGAGC 661 TGTACATTAA AAAAAATCAT CATTATTATT ATTATTTGCA GTCATGGAGA ACCACCTACC 721 CCTCTAGACC CAGCTTTCTT GTACAAAGTG GTGATGGCCG CGGATCCGCC CCTCTCCCTC 781 CCCCCCCCCT AACGTTACTG GCCGAAGCCG CTTGGAATAA GGCCGGTGTG CGTTTGTCTA 841 TATGTTATTT TCCACCATAT TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AANCTGGCCC 901 TGNCTTCTTG ACNANCATTC CNANGGGTCT TTCCCCNNNC GCCNAANGNA TGCNNGGNCT 961 GTTGANTGNC NNNNNNGANG CANTNNNNNT NGNNNNCNTN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Yoshitomi, H., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328. [link to RRC of NBRP]

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human MAF

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human MAF

Reference sequence