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pHISMBP-eIF4A1-NTD (Phe163Leu-Ile199Met)

Gateway based destinatin vector of human N-terminal region (NTD, 1-237) of eIF4A1 substitutions (F163L, I199M) tagged with 6xHis and MBP at N-terminus.

Catalog number RDB17305
Resource name pHISMBP-eIF4A1-NTD (Phe163Leu-Ile199Met)
Clone info. Gateway based destinatin vector of human N-terminal region (NTD, 1-237) of eukaryotic translation initiation factor 4A1 (eIF4A1) substitutions (F163L, I199M) tagged with 6xHis and MBP at N-terminus.
Expression was confirmed by the depositor with CBB staining.
Vector backbone pET-28a (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r.
Gene/insert name human EIF4A1 cDNA
Depositor Iwasaki, Shintaro |
 
Other clones in our bank human EIF4A1 (NCBI Gene 1973) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019).
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17305 pHISMBP-eIF4A1-NTD (Phe163Leu-Ile199Met) Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437.

2019.07.17

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