Gateway based destinatin vector of human N-terminal region (NTD, 1-237) of eIF4A1 substitutions (F163L, I199M) tagged with 6xHis and MBP at N-terminus.
Clone info. | Gateway based destinatin vector of human N-terminal region (NTD, 1-237) of eukaryotic translation initiation factor 4A1 (eIF4A1) substitutions (F163L, I199M) tagged with 6xHis and MBP at N-terminus. |
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Comment | Expression was confirmed by the depositor with CBB staining. |
Vector backbone | pET-28a (plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Kan^r. |
Gene/insert name | human EIF4A1 cDNA |
Depositor|Developer | Iwasaki, Shintaro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019). |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019)。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB17305 | pHISMBP-eIF4A1-NTD (Phe163Leu-Ile199Met) | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pHISMBP-eIF4A1-NTD (Phe163Leu-Ile199Met) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17305). |
Reference section:
Iwasaki, S., Iwasaki, W., Takahashi, M., Sakamoto, A., Watanabe, C., Shichino, Y., Floor, S.N., Fujiwara, K., Mito, M., Dodo, K., Sodeoka, M., Imataka, H., Honma, T., Fukuzawa, K., Ito, T., Ingolia, N.T., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437. [link to RRC of NBRP] |
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