Mammalian inducible expression vector of SBP-tagged human eIF4A1 substitutions (F163L, I199M) using with the Flp-InTM T-RExTM system.
Catalog number | RDB17298 |
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Resource name | pcDNA5/FRT/TO-SBP-eIF4A1 Phe163Leu-Ile199Met |
Clone info. | Mammalian inducible expression vector of streptavidin binding peptide (SBP)-tagged human eukaryotic translation initiation factor 4A1 (eIF4A1) substitutions (F163L, I199M) using with the Flp-InTM T-RExTM system. Expression was confirmed by the depositor with western blotting. |
Vector backbone | pcDNA5/FRT/TO (plasmid) |
Size of vector backbone | 5.1 kb |
Selectable markers | Amp^r (E. coli). |
Gene/insert name | human EIF4A1 cDNA |
Depositor | Iwasaki, Shintaro | |
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Other clones in our bank | human EIF4A1 (NCBI Gene 1973) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019). |
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Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB17298 | pcDNA5/FRT/TO-SBP-eIF4A1 Phe163Leu-Ile199Met | Under QC test. Please contact us. | DNA solution |
Electronic file
Original reference
original | Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437. |
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Further references such as user reports and related articles (go to bottom)
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This clone will be sequenced a portion and digested by restriction enzyme for examination.
Original, user report and related articles
original | Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437. |
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2019.07.17
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