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pcDNA5/FRT/TO-SBP-eIF4A1 Phe163Leu-Ile199Met (#RDB17298)

Mammalian inducible expression vector of SBP-tagged human eIF4A1 substitutions (F163L, I199M) using with the Flp-InTM T-RExTM system.

Clone info. Mammalian inducible expression vector of streptavidin binding peptide (SBP)-tagged human eukaryotic translation initiation factor 4A1 (eIF4A1) substitutions (F163L, I199M) using with the Flp-InTM T-RExTM system.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pcDNA5/FRT/TO (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli).
Gene/insert name human EIF4A1 cDNA
Depositor|Developer Iwasaki, Shintaro |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17298 pcDNA5/FRT/TO-SBP-eIF4A1 Phe163Leu-Ile199Met Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pcDNA5/FRT/TO-SBP-eIF4A1 Phe163Leu-Ile199Met was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17298).

Reference section:

Iwasaki, S., Iwasaki, W., Takahashi, M., Sakamoto, A., Watanabe, C., Shichino, Y., Floor, S.N., Fujiwara, K., Mito, M., Dodo, K., Sodeoka, M., Imataka, H., Honma, T., Fukuzawa, K., Ito, T., Ingolia, N.T., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437. [link to RRC of NBRP]