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pcDNA5/FRT/TO-SBP-eIF4A1 Phe163Leu-Ile199Met

Mammalian inducible expression vector of SBP-tagged human eIF4A1 substitutions (F163L, I199M) using with the Flp-InTM T-RExTM system.

Catalog number RDB17298
Resource name pcDNA5/FRT/TO-SBP-eIF4A1 Phe163Leu-Ile199Met
Clone info. Mammalian inducible expression vector of streptavidin binding peptide (SBP)-tagged human eukaryotic translation initiation factor 4A1 (eIF4A1) substitutions (F163L, I199M) using with the Flp-InTM T-RExTM system.
Expression was confirmed by the depositor with western blotting.
Vector backbone pcDNA5/FRT/TO (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli).
Gene/insert name human EIF4A1 cDNA
Depositor Iwasaki, Shintaro |
 
Other clones in our bank human EIF4A1 (NCBI Gene 1973) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019).
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17298 pcDNA5/FRT/TO-SBP-eIF4A1 Phe163Leu-Ile199Met Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

Original, user report and related articles

original Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437.

2019.07.17

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