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pcDNA5/FRT/TO-SBP-eIF4A1 Phe163Leu-Ile199Met

Mammalian inducible expression vector of SBP-tagged human eIF4A1 substitutions (F163L, I199M) using with the Flp-InTM T-RExTM system.

Catalog number RDB17298
Resource name pcDNA5/FRT/TO-SBP-eIF4A1 Phe163Leu-Ile199Met
Clone info. Mammalian inducible expression vector of streptavidin binding peptide (SBP)-tagged human eukaryotic translation initiation factor 4A1 (eIF4A1) substitutions (F163L, I199M) using with the Flp-InTM T-RExTM system.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pcDNA5/FRT/TO (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli).
Gene/insert name human EIF4A1 cDNA
Depositor|Developer Iwasaki, Shintaro |
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External Database
human EIF4A1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019)。
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          RDB17298 pcDNA5/FRT/TO-SBP-eIF4A1 Phe163Leu-Ile199Met Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437.

          2022.05.17

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