Mammalian inducible expression vector of SBP-tagged human eIF4A1, wild type using with the Flp-InTM T-RExTM system.
Clone info. | Mammalian inducible expression vector of streptavidin binding peptide (SBP)-tagged human eukaryotic translation initiation factor 4A1 (eIF4A1), wild type using with the Flp-InTM T-RExTM system. |
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Comment | Expression was confirmed by the depositor with western blotting. |
Vector backbone | pcDNA5/FRT/TO (plasmid) |
Size of vector backbone | 5.1 kb |
Selectable markers | Amp^r (E. coli). |
Gene/insert name | human EIF4A1 cDNA |
Depositor|Developer | Iwasaki, Shintaro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019). |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019)。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB17295 | pcDNA5/FRT/TO-SBP-eIF4A1 WT | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pcDNA5/FRT/TO-SBP-eIF4A1 WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17295). |
Reference section:
Iwasaki, S., Floor, S.N., Ingolia, N.T., Rocaglates convert DEAD-box protein eIF4A into a sequence-selective translational repressor. Nature 534 (7608): 558- 561 (2016). PMID 27309803. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Iwasaki, S., Rocaglates convert DEAD-box protein eIF4A into a sequence-selective translational repressor. Nature 534 (7608): 558- 561 (2016). PMID 27309803. [link to RRC of NBRP] |
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