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Lifeact-DsRed/pCAGEN (#RDB17291)

Expression vector of Lifeact fused with DsRed1, a versatile marker to visualize F-actin.

Clone info. Expression vector of Lifeact fused with DsRed1. Lifeact (MGVADLIKKFESISKEE), a versatile marker to visualize F-actin.
Vector backbone pCAGEN (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Depositor|Developer Kurabayashi, Nobuhiro |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kurabayashi N. et al. Development, 145(17), pii: dev162529, 2018.)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kurabayashi N. et al. Development, 145(17), pii: dev162529, 2018.)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17291 Lifeact-DsRed/pCAGEN DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Lifeact-DsRed/pCAGEN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17291).

Reference section:

Kurabayashi, N., Tanaka, A., Nguyen, M.D., Sanada, K., The LPA-LPA4 axis is required for establishment of bipolar morphology and radial migration of newborn cortical neurons. Development 145 (17). pii: dev162529 (2018). PMID 30217809. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17291_A9Dbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164)
Region: Lifeact,DsRed1 5'
Sequence file: RDB17291_A9Dba.seq check
>D06232A3_A9Db_3_pAxCA_F1_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTGCCTTC TTCTTTTTCC NACNGCTCCT GGGCAACGTG
   61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGAGCTCAA GCTTCGAATT
  121 ATGGGTGTCG CAGATTTGAT CAAGAAATTC GAAAGCATCT CAAAGGAAGA AGGGGATCCA
  181 CCGGTCGCCA CCATGGTGCG CTCCTCCAAG AACGTCATCA AGGAGTTCAT GCGCTTCAAG
  241 GTGCGCATGG AGGGCACCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC
  301 CGCCCCTACG AGGGCCACAA CACCGTGAAG CTGAAGGTGA CCAAGGGCGG CCCCCTGCCC
  361 TTCGCCTGGG ACATCCTGTC CCCCCAGTTC CAGTACGGCT CCAAGGTGTA CGTGAAGCAC
  421 CCCGCCGACA TCCCCGACTA CAAGAAGCTG TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC
  481 GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC CCTGCAGGAC
  541 GGCTGCTTCA TCTACAAGGT GAAGTTCATC GGCGTGAACT TCCCCTCCGA CGGCCCCGTA
  601 ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCACCGAGC GCCTGTACCC CCGCGACGGC
  661 GTGCTGAAGG GCGAGATCCA CAAGGCCCTG AAGCTGAAGG ACGGCGGCCA CTACCTGGTG
  721 GAGTTCAAGT CCATCTACAT GGCCAAGAAG CCCGTGCAGC TGCCCGGCTA CTACTACGTG
  781 GACTCCAAGC TGGACATCAC CTCCCACAAC GANGANTACA CCATCGTGGA NCAGTACGAG
  841 CGCACCGAGG GCCGCCACCA CCTGTTCCTG TANCGGCCGC ACTCCTCANG NGCANGCTGC
  901 CTATCANAAN GNGGTGNNTG GTGTGGNCAA TGNCCTNNNT CACAAATACN ACTGANATNT
  961 TTTTCCCNNT GNNNAAANTT ATGGGGNNNN TCATGNANNC NNNNTNNANC NTNNGNNNTN
1021 NNNGNNNNNN NNNN
//
Primer: pCAGGS_R1 (Pr0614)
Region: beta-globin pA,DsRed1 3'
Sequence file: RDB17291_A9Dbb.seq check
>D06232A3_A9Db_3_pCAGGS_R1_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN TTGCNNTGAA AATAAATTTC CTTTATTAGC CAGAAGTCAG
   61 ATGCTCAAGG GGCTTCATGA TGTCCCCATA ATTTTTGGCA GAGGGAAAAA GATCTCAGTG
  121 GTATTTGTGA GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC
  181 CTGAGGAGTG CGGCCGCTAC AGGAACAGGT GGTGGCGGCC CTCGGTGCGC TCGTACTGCT
  241 CCACGATGGT GTAGTCCTCG TTGTGGGAGG TGATGTCCAG CTTGGAGTCC ACGTAGTAGT
  301 AGCCGGGCAG CTGCACGGGC TTCTTGGCCA TGTAGATGGA CTTGAACTCC ACCAGGTAGT
  361 GGCCGCCGTC CTTCAGCTTC AGGGCCTTGT GGATCTCGCC CTTCAGCACG CCGTCGCGGG
  421 GGTACAGGCG CTCGGTGGAG GCCTCCCAGC CCATGGTCTT CTTCTGCATT ACGGGGCCGT
  481 CGGAGGGGAA GTTCACGCCG ATGAACTTCA CCTTGTAGAT GAAGCAGCCG TCCTGCAGGG
  541 AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA GTTCATCACG CGCTCCCACT
  601 TGAAGCCCTC GGGGAAGGAC AGCTTCTTGT AGTCGGGGAT GTCGGCGGGG TGCTTCACGT
  661 ACACCTTGGA GCCGTACTGG AACTGGGGGG ACAGGATGTC CCAGGCGAAG GGCAGGGGGC
  721 CGCCCTTGGT CACCTTCAGC TTCACGGTGT TGTGNNCCTC GTANGGGCGG CCCTCGCCCT
  781 CGCCCTCGAT CTCGAACTCG TGGCCGTTCA CGGTGCCCTC CNTGCGCACC TTGAAGCGCA
  841 TGAACTCCTT GATGANGTTC TTGGANGANC GCACCATGGT GGCGNNCGGN GGNTNCCCTT
  901 CTTCCTTTGA NANGCTTTCG AATTTCTTGA TNAANTCTGC NNNNCCNNTA ANTCNAAGCT
  961 NNAGCTCNAN GNANNTCTTT NNCNNNANTG NTGANNNAGC NNNNNAANCN GCANNNTNNN
1021 NNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori,SV40pA,ori
Sequence file: RDB17291_A9Dbc.seq check
>D06232A3_A9Db_3_SV40pro_F_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGTGAG GNNGNTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA
  121 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA
  181 TGTATCTTAT CATGTCTGGA TCCGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC
  241 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT
  301 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA
  361 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA
  421 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
  481 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
  541 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC
  601 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT
  661 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC
  721 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG
  781 CCACTGGCAG CAGCCACTGG TAACAGGANT AGCAGANCGA GGTATGTANG CGGTGCTACA
  841 GAGTTCTTGA AGTGGTGGCC TAACTACNGC TACNCTAGAA GAACAGTANT TGGTATCTGC
  901 GCTCTGCTGA ANCCNGTTAC NTTNNGNAAA ANAGTTGGTA NCTCTTGATC CGGNNAANNN
961 NNANNGCNNG TANCGGNGGN TTTTTTTGNT TNNNNNCNGC NNNNTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kurabayashi, N., The LPA-LPA4 axis is required for establishment of bipolar morphology and radial migration of newborn cortical neurons. Development 145 (17). pii: dev162529 (2018). PMID 30217809. [link to RRC of NBRP]